Restriction map for
pF1KB4590
(Linear) (Six-base) MAPSORT of: KIBB4590.seq Check: 7609 from: 1 to: 2019
KIBB4590.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 174 2019
Size: 174 1845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 47 407 928 935 2019
Size: 29 18 360 521 7 1084
Fragments arranged by size:
1084 521 360 29 18 7
AflII C'TTAA_G
Cuts at: 0 244 2019
Size: 244 1775
AflIII A'CryG_T
Cuts at: 0 1958 2019
Size: 1958 61
AleI CACnn'nnGTG
Cuts at: 0 1972 2019
Size: 1972 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 977 2019
Size: 977 1042
AlwNI CAG_nnn'CTG
Cuts at: 0 1917 2019
Size: 1917 102
ApoI r'AATT_y
Cuts at: 0 1078 1340 1540 1847 2019
Size: 1078 262 200 307 172
Fragments arranged by size:
1078 307 262 200 172
BamHI G'GATC_C
Cuts at: 0 1009 2019
Size: 1009 1010
BanI G'GyrC_C
Cuts at: 0 679 2019
Size: 679 1340
BanII G_rGCy'C
Cuts at: 0 1662 2019
Size: 1662 357
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 551 646 2019
Size: 104 447 95 1373
Fragments arranged by size:
1373 447 104 95
BbvCI CC'TCA_GC
Cuts at: 0 2003 2019
Size: 2003 16
BclI T'GATC_A
Cuts at: 0 1880 1902 2019
Size: 1880 22 117
Fragments arranged by size:
1880 117 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1170 2019
Size: 1170 849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1481 1626 2019
Size: 721 760 145 393
Fragments arranged by size:
760 721 393 145
Bpu10I CC'TnA_GC
Cuts at: 0 374 800 1922 2003 2019
Size: 374 426 1122 81 16
Fragments arranged by size:
1122 426 374 81 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 571 1644 2019
Size: 571 1073 375
Fragments arranged by size:
1073 571 375
BsaBI GATnn'nnATC
Cuts at: 0 1901 2019
Size: 1901 118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 302 575 2019
Size: 139 163 273 1444
Fragments arranged by size:
1444 273 163 139
BseYI C'CCAG_C
Cuts at: 0 140 1260 1711 2019
Size: 140 1120 451 308
Fragments arranged by size:
1120 451 308 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1117 2019
Size: 610 507 902
Fragments arranged by size:
902 610 507
BsiHKAI G_wGCw'C
Cuts at: 0 1662 2019
Size: 1662 357
Bsp1286I G_dGCh'C
Cuts at: 0 1662 2019
Size: 1662 357
BspHI T'CATG_A
Cuts at: 0 1905 2019
Size: 1905 114
BsrDI GCAATG_nn'
Cuts at: 0 959 1748 2019
Size: 959 789 271
Fragments arranged by size:
959 789 271
BsrFI r'CCGG_y
Cuts at: 0 1380 2019
Size: 1380 639
BstYI r'GATC_y
Cuts at: 0 819 1009 2019
Size: 819 190 1010
Fragments arranged by size:
1010 819 190
BtgI C'CryG_G
Cuts at: 0 238 859 1754 2019
Size: 238 621 895 265
Fragments arranged by size:
895 621 265 238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1958 1993 2019
Size: 1958 35 26
Fragments arranged by size:
1958 35 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 1533 1963 2019
Size: 1533 430 56
Fragments arranged by size:
1533 430 56
EaeI y'GGCC_r
Cuts at: 0 275 969 1324 1554 2019
Size: 275 694 355 230 465
Fragments arranged by size:
694 465 355 275 230
EarI CTCTTCn'nnn_
Cuts at: 0 181 515 1109 2019
Size: 181 334 594 910
Fragments arranged by size:
910 594 334 181
EcoICRI GAG'CTC
Cuts at: 0 1660 2019
Size: 1660 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 47 407 721 928 935 1481 1626
Size: 29 18 360 314 207 7 546 145
Cuts at: 1626 2019
Size: 393
Fragments arranged by size:
546 393 360 314 207 145 29 18
7
EcoRI G'AATT_C
Cuts at: 0 1078 2019
Size: 1078 941
EcoRV GAT'ATC
Cuts at: 0 921 2019
Size: 921 1098
HaeII r_GCGC'y
Cuts at: 0 1709 2019
Size: 1709 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 949 981 984 1016 1292 1324
Size: 604 32 313 32 3 32 276 32
Cuts at: 1324 2019
Size: 695
Fragments arranged by size:
695 604 313 276 32 32 32 32
3
HincII GTy'rAC
Cuts at: 0 1218 2019
Size: 1218 801
MfeI C'AATT_G
Cuts at: 0 907 2019
Size: 907 1112
MscI TGG'CCA
Cuts at: 0 277 2019
Size: 277 1742
MslI CAynn'nnrTG
Cuts at: 0 1753 1972 2019
Size: 1753 219 47
Fragments arranged by size:
1753 219 47
MspA1I CmG'CkG
Cuts at: 0 157 692 2019
Size: 157 535 1327
Fragments arranged by size:
1327 535 157
NcoI C'CATG_G
Cuts at: 0 238 859 2019
Size: 238 621 1160
Fragments arranged by size:
1160 621 238
NspI r_CATG'y
Cuts at: 0 712 1962 2019
Size: 712 1250 57
Fragments arranged by size:
1250 712 57
PciI A'CATG_T
Cuts at: 0 1958 2019
Size: 1958 61
PflMI CCAn_nnn'nTGG
Cuts at: 0 1917 2019
Size: 1917 102
PfoI T'CCnGG_A
Cuts at: 0 1642 2019
Size: 1642 377
PshAI GACnn'nnGTC
Cuts at: 0 1682 2019
Size: 1682 337
PstI C_TGCA'G
Cuts at: 0 156 1025 2019
Size: 156 869 994
Fragments arranged by size:
994 869 156
PvuII CAG'CTG
Cuts at: 0 157 692 2019
Size: 157 535 1327
Fragments arranged by size:
1327 535 157
SacI G_AGCT'C
Cuts at: 0 1662 2019
Size: 1662 357
SbfI CC_TGCA'GG
Cuts at: 0 1025 2019
Size: 1025 994
SfcI C'TryA_G
Cuts at: 0 152 1021 1482 1548 2019
Size: 152 869 461 66 471
Fragments arranged by size:
869 471 461 152 66
SmlI C'TyrA_G
Cuts at: 0 244 2019
Size: 244 1775
StyI C'CwwG_G
Cuts at: 0 68 238 581 713 859 1232 1529 2019
Size: 68 170 343 132 146 373 297 490
Fragments arranged by size:
490 373 343 297 170 146 132 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1576 1953 2019
Size: 1576 377 66
Fragments arranged by size:
1576 377 66
XbaI T'CTAG_A
Cuts at: 0 1650 2019
Size: 1650 369
XmnI GAAnn'nnTTC
Cuts at: 0 350 2019
Size: 350 1669
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AlwNI ApoI
BamHI BanI BanII BbsI BbvCI BclI BmrI BpmI
Bpu10I BsaI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrDI BsrFI BstYI BtgI CspCI DrdI EaeI
EarI EcoICRI Eco57MI EcoRI EcoRV HaeII Hin4I HincII
MfeI MscI MslI MspA1I NcoI NspI PciI PflMI
PfoI PshAI PstI PvuII SacI SbfI SfcI SmlI
StyI TaqII XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BpuEI BsaAI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII EagI EciI EcoNI
EcoO109I FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XcmI XhoI XmaI ZraI