Restriction map for
pF1KB4594
(Linear) (Six-base) MAPSORT of: KIBB4594.seq Check: 352 from: 1 to: 2118
KIBB4594.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1242 1952 2118
Size: 1242 710 166
Fragments arranged by size:
1242 710 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1909 1954 2118
Size: 1909 45 164
Fragments arranged by size:
1909 164 45
AgeI A'CCGG_T
Cuts at: 0 1705 2118
Size: 1705 413
AhdI GACnn_n'nnGTC
Cuts at: 0 1275 2118
Size: 1275 843
AleI CACnn'nnGTG
Cuts at: 0 824 1850 1874 2062 2118
Size: 824 1026 24 188 56
Fragments arranged by size:
1026 824 188 56 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1479 2118
Size: 1479 639
AlwNI CAG_nnn'CTG
Cuts at: 0 715 1932 2086 2118
Size: 715 1217 154 32
Fragments arranged by size:
1217 715 154 32
ApaI G_GGCC'C
Cuts at: 0 1743 2118
Size: 1743 375
ApaLI G'TGCA_C
Cuts at: 0 1422 2118
Size: 1422 696
ApoI r'AATT_y
Cuts at: 0 1781 2118
Size: 1781 337
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6 39 2118
Size: 6 33 2079
Fragments arranged by size:
2079 33 6
BanI G'GyrC_C
Cuts at: 0 1906 1919 2044 2118
Size: 1906 13 125 74
Fragments arranged by size:
1906 125 74 13
BanII G_rGCy'C
Cuts at: 0 135 953 1000 1099 1369 1743 2105 2118
Size: 135 818 47 99 270 374 362 13
Fragments arranged by size:
818 374 362 270 135 99 47 13
BbeI G_GCGC'C
Cuts at: 0 2048 2118
Size: 2048 70
BbvCI CC'TCA_GC
Cuts at: 0 833 858 2118
Size: 833 25 1260
Fragments arranged by size:
1260 833 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 34 2118
Size: 34 2084
BclI T'GATC_A
Cuts at: 0 212 2118
Size: 212 1906
BglI GCCn_nnn'nGGC
Cuts at: 0 10 1225 2118
Size: 10 1215 893
Fragments arranged by size:
1215 893 10
BlpI GC'TnA_GC
Cuts at: 0 1193 2118
Size: 1193 925
Bme1580I G_kGCm'C
Cuts at: 0 687 1426 1743 1857 2118
Size: 687 739 317 114 261
Fragments arranged by size:
739 687 317 261 114
BmgBI CAC'GTC
Cuts at: 0 1427 2118
Size: 1427 691
BmrI ACTGGGnnnn_n'
Cuts at: 0 1506 1737 2118
Size: 1506 231 381
Fragments arranged by size:
1506 381 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 933 1410 1608 2118
Size: 933 477 198 510
Fragments arranged by size:
933 510 477 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1070 1258 1811 2118
Size: 1051 19 188 553 307
Fragments arranged by size:
1051 553 307 188 19
Bpu10I CC'TnA_GC
Cuts at: 0 833 858 1184 2118
Size: 833 25 326 934
Fragments arranged by size:
934 833 326 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1582 2118
Size: 1582 536
BsaI GGTCTCn'nnnn_
Cuts at: 0 906 1501 2118
Size: 906 595 617
Fragments arranged by size:
906 617 595
BsaHI Gr'CG_yC
Cuts at: 0 2045 2118
Size: 2045 73
BsaWI w'CCGG_w
Cuts at: 0 74 647 971 1705 2097 2118
Size: 74 573 324 734 392 21
Fragments arranged by size:
734 573 392 324 74 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1353 1383 2118
Size: 1353 30 735
Fragments arranged by size:
1353 735 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 404 522 607 646 909 1510 1600 2118
Size: 404 118 85 39 263 601 90 518
Fragments arranged by size:
601 518 404 263 118 90 85 39
BseYI C'CCAG_C
Cuts at: 0 544 1197 2118
Size: 544 653 921
Fragments arranged by size:
921 653 544
BsiEI CG_ry'CG
Cuts at: 0 733 2118
Size: 733 1385
BsiHKAI G_wGCw'C
Cuts at: 0 1148 1426 2105 2118
Size: 1148 278 679 13
Fragments arranged by size:
1148 679 278 13
BsmI GAATG_Cn'
Cuts at: 0 1059 1170 2118
Size: 1059 111 948
Fragments arranged by size:
1059 948 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 76 2118
Size: 76 2042
Bsp1286I G_dGCh'C
Cuts at: 0 135 687 953 1000 1099 1148 1369 1426
Size: 135 552 266 47 99 49 221 57
Cuts at: 1426 1743 1857 2105 2118
Size: 317 114 248 13
Fragments arranged by size:
552 317 266 248 221 135 114 99
57 49 47 13
BspEI T'CCGG_A
Cuts at: 0 74 647 2097 2118
Size: 74 573 1450 21
Fragments arranged by size:
1450 573 74 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 506 855 1242 1825 1952 2000 2099
Size: 341 165 349 387 583 127 48 99
Cuts at: 2099 2118
Size: 19
Fragments arranged by size:
583 387 349 341 165 127 99 48
19
BsrBI CCG'CTC
Cuts at: 0 681 2118
Size: 681 1437
BsrDI GCAATG_nn'
Cuts at: 0 1566 2118
Size: 1566 552
BsrFI r'CCGG_y
Cuts at: 0 9 728 1331 1705 1751 2118
Size: 9 719 603 374 46 367
Fragments arranged by size:
719 603 374 367 46 9
BsrGI T'GTAC_A
Cuts at: 0 269 551 590 1833 2118
Size: 269 282 39 1243 285
Fragments arranged by size:
1243 285 282 269 39
BssSI C'ACGA_G
Cuts at: 0 416 1214 2118
Size: 416 798 904
Fragments arranged by size:
904 798 416
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1430 1611 2118
Size: 1430 181 507
Fragments arranged by size:
1430 507 181
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1075 1477 2041 2118
Size: 1075 402 564 77
Fragments arranged by size:
1075 564 402 77
BstYI r'GATC_y
Cuts at: 0 222 2118
Size: 222 1896
Bsu36I CC'TnA_GG
Cuts at: 0 239 1493 2118
Size: 239 1254 625
Fragments arranged by size:
1254 625 239
BtgI C'CryG_G
Cuts at: 0 320 1250 2118
Size: 320 930 868
Fragments arranged by size:
930 868 320
BtsI GCAGTG_nn'
Cuts at: 0 165 2118
Size: 165 1953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1716 1751 2118
Size: 1716 35 367
Fragments arranged by size:
1716 367 35
DraIII CAC_nnn'GTG
Cuts at: 0 811 2118
Size: 811 1307
EaeI y'GGCC_r
Cuts at: 0 317 659 730 1753 2118
Size: 317 342 71 1023 365
Fragments arranged by size:
1023 365 342 317 71
EagI C'GGCC_G
Cuts at: 0 730 2118
Size: 730 1388
EarI CTCTTCn'nnn_
Cuts at: 0 416 625 794 902 1930 2118
Size: 416 209 169 108 1028 188
Fragments arranged by size:
1028 416 209 188 169 108
EcoICRI GAG'CTC
Cuts at: 0 2103 2118
Size: 2103 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1070 1258 1811 1909 1954 2118
Size: 1051 19 188 553 98 45 164
Fragments arranged by size:
1051 553 188 164 98 45 19
EcoO109I rG'GnC_Cy
Cuts at: 0 837 1180 1547 1739 2118
Size: 837 343 367 192 379
Fragments arranged by size:
837 379 367 343 192
EcoRI G'AATT_C
Cuts at: 0 1781 2118
Size: 1781 337
HaeII r_GCGC'y
Cuts at: 0 515 2048 2118
Size: 515 1533 70
Fragments arranged by size:
1533 515 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 1261 1293 1353 1385 1498 1530
Size: 236 32 993 32 60 32 113 32
Cuts at: 1530 2118
Size: 588
Fragments arranged by size:
993 588 236 113 60 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1350 2118
Size: 1350 768
HpaI GTT'AAC
Cuts at: 0 1350 2118
Size: 1350 768
KasI G'GCGC_C
Cuts at: 0 2044 2118
Size: 2044 74
MscI TGG'CCA
Cuts at: 0 319 661 2118
Size: 319 342 1457
Fragments arranged by size:
1457 342 319
MslI CAynn'nnrTG
Cuts at: 0 44 109 310 325 690 824 1706 1850
Size: 44 65 201 15 365 134 882 144
Cuts at: 1850 1874 2062 2118
Size: 24 188 56
Fragments arranged by size:
882 365 201 188 144 134 65 56
44 24 15
MspA1I CmG'CkG
Cuts at: 0 20 1301 1477 1649 2016 2118
Size: 20 1281 176 172 367 102
Fragments arranged by size:
1281 367 176 172 102 20
NaeI GCC'GGC
Cuts at: 0 11 730 1333 2118
Size: 11 719 603 785
Fragments arranged by size:
785 719 603 11
NarI GG'CG_CC
Cuts at: 0 2045 2118
Size: 2045 73
NcoI C'CATG_G
Cuts at: 0 1250 2118
Size: 1250 868
NgoMIV G'CCGG_C
Cuts at: 0 9 728 1331 2118
Size: 9 719 603 787
Fragments arranged by size:
787 719 603 9
NspI r_CATG'y
Cuts at: 0 349 1410 1456 2118
Size: 349 1061 46 662
Fragments arranged by size:
1061 662 349 46
PasI CC'CwG_GG
Cuts at: 0 517 2118
Size: 517 1601
PflMI CCAn_nnn'nTGG
Cuts at: 0 715 2118
Size: 715 1403
PfoI T'CCnGG_A
Cuts at: 0 257 2118
Size: 257 1861
PspOMI G'GGCC_C
Cuts at: 0 1739 2118
Size: 1739 379
PstI C_TGCA'G
Cuts at: 0 160 338 1045 1616 2118
Size: 160 178 707 571 502
Fragments arranged by size:
707 571 502 178 160
PvuII CAG'CTG
Cuts at: 0 20 2016 2118
Size: 20 1996 102
Fragments arranged by size:
1996 102 20
SacI G_AGCT'C
Cuts at: 0 2105 2118
Size: 2105 13
SapI GCTCTTCn'nnn_
Cuts at: 0 1930 2118
Size: 1930 188
ScaI AGT'ACT
Cuts at: 0 1522 2118
Size: 1522 596
SfcI C'TryA_G
Cuts at: 0 156 171 334 822 1041 1612 2118
Size: 156 15 163 488 219 571 506
Fragments arranged by size:
571 506 488 219 163 156 15
SfoI GGC'GCC
Cuts at: 0 2046 2118
Size: 2046 72
SmlI C'TyrA_G
Cuts at: 0 1597 2118
Size: 1597 521
SphI G_CATG'C
Cuts at: 0 1456 2118
Size: 1456 662
StuI AGG'CCT
Cuts at: 0 1591 1657 2118
Size: 1591 66 461
Fragments arranged by size:
1591 461 66
StyI C'CwwG_G
Cuts at: 0 674 782 1250 2060 2118
Size: 674 108 468 810 58
Fragments arranged by size:
810 674 468 108 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 509 795 1263 2118
Size: 509 286 468 855
Fragments arranged by size:
855 509 468 286
TatI w'GTAC_w
Cuts at: 0 269 551 590 722 1520 1833 2118
Size: 269 282 39 132 798 313 285
Fragments arranged by size:
798 313 285 282 269 132 39
Tth111I GACn'n_nGTC
Cuts at: 0 1512 2118
Size: 1512 606
XbaI T'CTAG_A
Cuts at: 0 219 2118
Size: 219 1899
Enzymes that do cut:
AarI AcuI AgeI AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI BaeI BanI BanII BbeI BbvCI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HpaI KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PspOMI PstI PvuII SacI SapI
ScaI SfcI SfoI SmlI SphI StuI StyI TaqII
TatI Tth111I XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AloI
AscI AseI AsiSI AvaI AvrII BamHI BbsI BcgI
BfrBI BglII BmtI BsaAI BsaBI BsgI BsiWI BspHI
BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DrdI
EciI EcoNI EcoRV FalI FseI FspI FspAI HindIII
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TstI XcmI XhoI XmaI XmnI
ZraI