Restriction map for
pF1KB4609
(Linear) (Six-base) MAPSORT of: KIBB4609.seq Check: 456 from: 1 to: 834
KIBB4609.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 561 786 834
Size: 304 257 225 48
Fragments arranged by size:
304 257 225 48
AfeI AGC'GCT
Cuts at: 0 92 424 834
Size: 92 332 410
Fragments arranged by size:
410 332 92
AhdI GACnn_n'nnGTC
Cuts at: 0 49 834
Size: 49 785
AleI CACnn'nnGTG
Cuts at: 0 58 834
Size: 58 776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 700 834
Size: 700 134
AlwNI CAG_nnn'CTG
Cuts at: 0 164 486 834
Size: 164 322 348
Fragments arranged by size:
348 322 164
ApaLI G'TGCA_C
Cuts at: 0 589 619 834
Size: 589 30 215
Fragments arranged by size:
589 215 30
AseI AT'TA_AT
Cuts at: 0 269 834
Size: 269 565
BamHI G'GATC_C
Cuts at: 0 198 834
Size: 198 636
BanI G'GyrC_C
Cuts at: 0 406 834
Size: 406 428
BanII G_rGCy'C
Cuts at: 0 137 834
Size: 137 697
BbeI G_GCGC'C
Cuts at: 0 410 834
Size: 410 424
BbvCI CC'TCA_GC
Cuts at: 0 216 834
Size: 216 618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 765 799 834
Size: 765 34 35
Fragments arranged by size:
765 35 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 222 834
Size: 222 612
BfrBI ATG'CAT
Cuts at: 0 630 834
Size: 630 204
BglI GCCn_nnn'nGGC
Cuts at: 0 314 368 834
Size: 314 54 466
Fragments arranged by size:
466 314 54
Bme1580I G_kGCm'C
Cuts at: 0 593 623 834
Size: 593 30 211
Fragments arranged by size:
593 211 30
Bpu10I CC'TnA_GC
Cuts at: 0 10 216 834
Size: 10 206 618
Fragments arranged by size:
618 206 10
BsaAI yAC'GTr
Cuts at: 0 618 834
Size: 618 216
BsaHI Gr'CG_yC
Cuts at: 0 407 834
Size: 407 427
BseYI C'CCAG_C
Cuts at: 0 667 834
Size: 667 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 834
Size: 445 389
BsiEI CG_ry'CG
Cuts at: 0 234 539 834
Size: 234 305 295
Fragments arranged by size:
305 295 234
BsiHKAI G_wGCw'C
Cuts at: 0 137 593 623 834
Size: 137 456 30 211
Fragments arranged by size:
456 211 137 30
BsiWI C'GTAC_G
Cuts at: 0 614 834
Size: 614 220
BsmBI CGTCTCn'nnnn_
Cuts at: 0 604 834
Size: 604 230
Bsp1286I G_dGCh'C
Cuts at: 0 137 593 623 834
Size: 137 456 30 211
Fragments arranged by size:
456 211 137 30
BsrFI r'CCGG_y
Cuts at: 0 538 834
Size: 538 296
BstAPI GCAn_nnn'nTGC
Cuts at: 0 627 834
Size: 627 207
BstBI TT'CG_AA
Cuts at: 0 494 834
Size: 494 340
BstEII G'GTnAC_C
Cuts at: 0 51 834
Size: 51 783
BstYI r'GATC_y
Cuts at: 0 119 198 834
Size: 119 79 636
Fragments arranged by size:
636 119 79
Bsu36I CC'TnA_GG
Cuts at: 0 365 834
Size: 365 469
BtgI C'CryG_G
Cuts at: 0 56 834
Size: 56 778
BtsI GCAGTG_nn'
Cuts at: 0 10 834
Size: 10 824
EaeI y'GGCC_r
Cuts at: 0 155 231 540 807 834
Size: 155 76 309 267 27
Fragments arranged by size:
309 267 155 76 27
EagI C'GGCC_G
Cuts at: 0 231 834
Size: 231 603
EarI CTCTTCn'nnn_
Cuts at: 0 228 496 748 834
Size: 228 268 252 86
Fragments arranged by size:
268 252 228 86
EcoICRI GAG'CTC
Cuts at: 0 135 834
Size: 135 699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 561 786 834
Size: 304 257 225 48
Fragments arranged by size:
304 257 225 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 817 834
Size: 817 17
EcoO109I rG'GnC_Cy
Cuts at: 0 76 189 300 343 834
Size: 76 113 111 43 491
Fragments arranged by size:
491 113 111 76 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 444 834
Size: 444 390
HaeII r_GCGC'y
Cuts at: 0 94 410 426 753 834
Size: 94 316 16 327 81
Fragments arranged by size:
327 316 94 81 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 834
Size: 35 32 767
Fragments arranged by size:
767 35 32
KasI G'GCGC_C
Cuts at: 0 406 834
Size: 406 428
MscI TGG'CCA
Cuts at: 0 157 834
Size: 157 677
MslI CAynn'nnrTG
Cuts at: 0 58 834
Size: 58 776
MspA1I CmG'CkG
Cuts at: 0 161 467 596 806 834
Size: 161 306 129 210 28
Fragments arranged by size:
306 210 161 129 28
NarI GG'CG_CC
Cuts at: 0 407 834
Size: 407 427
NcoI C'CATG_G
Cuts at: 0 56 834
Size: 56 778
NsiI A_TGCA'T
Cuts at: 0 632 834
Size: 632 202
PasI CC'CwG_GG
Cuts at: 0 72 834
Size: 72 762
PpuMI rG'GwC_Cy
Cuts at: 0 76 189 300 343 834
Size: 76 113 111 43 491
Fragments arranged by size:
491 113 111 76 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 754 786 834
Size: 754 32 48
Fragments arranged by size:
754 48 32
PvuII CAG'CTG
Cuts at: 0 596 834
Size: 596 238
SacI G_AGCT'C
Cuts at: 0 137 834
Size: 137 697
SanDI GG'GwC_CC
Cuts at: 0 76 300 343 834
Size: 76 224 43 491
Fragments arranged by size:
491 224 76 43
SapI GCTCTTCn'nnn_
Cuts at: 0 496 748 834
Size: 496 252 86
Fragments arranged by size:
496 252 86
SfcI C'TryA_G
Cuts at: 0 771 834
Size: 771 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 314 834
Size: 314 520
SfoI GGC'GCC
Cuts at: 0 408 834
Size: 408 426
StyI C'CwwG_G
Cuts at: 0 56 348 834
Size: 56 292 486
Fragments arranged by size:
486 292 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 509 723 834
Size: 509 214 111
Fragments arranged by size:
509 214 111
Tth111I GACn'n_nGTC
Cuts at: 0 727 834
Size: 727 107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 355 834
Size: 355 479
XmnI GAAnn'nnTTC
Cuts at: 0 424 834
Size: 424 410
Enzymes that do cut:
AcuI AfeI AhdI AleI AlfI AlwNI ApaLI AseI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI
BglI Bme1580I Bpu10I BsaAI BsaHI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BsrFI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MscI
MslI MspA1I NarI NcoI NsiI PasI PpuMI PsrI
PvuII SacI SanDI SapI SfcI SfiI SfoI StyI
TaqII Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AloI ApaI ApoI AscI AsiSI AvaI AvrII BaeI
BbsI BclI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI RsrII SacII SalI SbfI ScaI SexAI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI XbaI XhoI XmaI ZraI