Restriction map for
pF1KB4614
(Linear) (Six-base) MAPSORT of: KIBB4614.seq Check: 1240 from: 1 to: 1788
KIBB4614.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 914 1788
Size: 824 90 874
Fragments arranged by size:
874 824 90
AfeI AGC'GCT
Cuts at: 0 585 736 1137 1788
Size: 585 151 401 651
Fragments arranged by size:
651 585 401 151
AflIII A'CryG_T
Cuts at: 0 1646 1788
Size: 1646 142
AleI CACnn'nnGTG
Cuts at: 0 631 1210 1788
Size: 631 579 578
Fragments arranged by size:
631 579 578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 560 1788
Size: 560 1228
ApoI r'AATT_y
Cuts at: 0 1327 1417 1624 1782 1788
Size: 1327 90 207 158 6
Fragments arranged by size:
1327 207 158 90 6
AvaI C'yCGr_G
Cuts at: 0 979 1788
Size: 979 809
BamHI G'GATC_C
Cuts at: 0 679 1057 1788
Size: 679 378 731
Fragments arranged by size:
731 679 378
BanI G'GyrC_C
Cuts at: 0 381 1158 1316 1788
Size: 381 777 158 472
Fragments arranged by size:
777 472 381 158
BanII G_rGCy'C
Cuts at: 0 1392 1788
Size: 1392 396
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 812 1788
Size: 398 414 976
Fragments arranged by size:
976 414 398
BciVI GTATCCnnnnn_n'
Cuts at: 0 792 1788
Size: 792 996
BlpI GC'TnA_GC
Cuts at: 0 1223 1788
Size: 1223 565
Bme1580I G_kGCm'C
Cuts at: 0 323 1788
Size: 323 1465
BmrI ACTGGGnnnn_n'
Cuts at: 0 878 1312 1788
Size: 878 434 476
Fragments arranged by size:
878 476 434
BmtI G_CTAG'C
Cuts at: 0 661 1788
Size: 661 1127
Bpu10I CC'TnA_GC
Cuts at: 0 269 1788
Size: 269 1519
BsaHI Gr'CG_yC
Cuts at: 0 813 1067 1788
Size: 813 254 721
Fragments arranged by size:
813 721 254
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 1393 1788
Size: 500 893 395
Fragments arranged by size:
893 500 395
BseYI C'CCAG_C
Cuts at: 0 30 453 1187 1788
Size: 30 423 734 601
Fragments arranged by size:
734 601 423 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 560 1788
Size: 242 318 1228
Fragments arranged by size:
1228 318 242
BsiHKAI G_wGCw'C
Cuts at: 0 1229 1788
Size: 1229 559
Bsp1286I G_dGCh'C
Cuts at: 0 323 1229 1392 1788
Size: 323 906 163 396
Fragments arranged by size:
906 396 323 163
BsrFI r'CCGG_y
Cuts at: 0 273 1788
Size: 273 1515
BsrGI T'GTAC_A
Cuts at: 0 217 1788
Size: 217 1571
BstAPI GCAn_nnn'nTGC
Cuts at: 0 256 1788
Size: 256 1532
BstEII G'GTnAC_C
Cuts at: 0 1017 1788
Size: 1017 771
BstXI CCAn_nnnn'nTGG
Cuts at: 0 38 868 1788
Size: 38 830 920
Fragments arranged by size:
920 830 38
BstYI r'GATC_y
Cuts at: 0 679 1057 1454 1788
Size: 679 378 397 334
Fragments arranged by size:
679 397 378 334
BtgI C'CryG_G
Cuts at: 0 385 494 610 1614 1788
Size: 385 109 116 1004 174
Fragments arranged by size:
1004 385 174 116 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 155 1788
Size: 155 1633
BtsI GCAGTG_nn'
Cuts at: 0 1341 1788
Size: 1341 447
DraIII CAC_nnn'GTG
Cuts at: 0 729 1788
Size: 729 1059
EaeI y'GGCC_r
Cuts at: 0 1093 1788
Size: 1093 695
EarI CTCTTCn'nnn_
Cuts at: 0 669 778 1788
Size: 669 109 1010
Fragments arranged by size:
1010 669 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 914 1788
Size: 824 90 874
Fragments arranged by size:
874 824 90
EcoRI G'AATT_C
Cuts at: 0 1327 1788
Size: 1327 461
HaeII r_GCGC'y
Cuts at: 0 587 738 1139 1788
Size: 587 151 401 649
Fragments arranged by size:
649 587 401 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1454 1486 1788
Size: 1454 32 302
Fragments arranged by size:
1454 302 32
MslI CAynn'nnrTG
Cuts at: 0 542 631 770 1210 1619 1788
Size: 542 89 139 440 409 169
Fragments arranged by size:
542 440 409 169 139 89
MspA1I CmG'CkG
Cuts at: 0 1039 1788
Size: 1039 749
NcoI C'CATG_G
Cuts at: 0 1614 1788
Size: 1614 174
NheI G'CTAG_C
Cuts at: 0 657 1788
Size: 657 1131
NspI r_CATG'y
Cuts at: 0 78 481 541 978 1498 1650 1771 1788
Size: 78 403 60 437 520 152 121 17
Fragments arranged by size:
520 437 403 152 121 78 60 17
PasI CC'CwG_GG
Cuts at: 0 684 1788
Size: 684 1104
PciI A'CATG_T
Cuts at: 0 1646 1788
Size: 1646 142
PflMI CCAn_nnn'nTGG
Cuts at: 0 823 1003 1788
Size: 823 180 785
Fragments arranged by size:
823 785 180
PfoI T'CCnGG_A
Cuts at: 0 1652 1788
Size: 1652 136
PshAI GACnn'nnGTC
Cuts at: 0 812 1788
Size: 812 976
PstI C_TGCA'G
Cuts at: 0 56 600 1038 1788
Size: 56 544 438 750
Fragments arranged by size:
750 544 438 56
PvuII CAG'CTG
Cuts at: 0 1039 1788
Size: 1039 749
SexAI A'CCwGG_T
Cuts at: 0 1271 1788
Size: 1271 517
SfcI C'TryA_G
Cuts at: 0 9 52 596 1034 1052 1788
Size: 9 43 544 438 18 736
Fragments arranged by size:
736 544 438 43 18 9
SphI G_CATG'C
Cuts at: 0 541 978 1788
Size: 541 437 810
Fragments arranged by size:
810 541 437
StyI C'CwwG_G
Cuts at: 0 37 817 1101 1458 1614 1788
Size: 37 780 284 357 156 174
Fragments arranged by size:
780 357 284 174 156 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 276 1334 1788
Size: 276 1058 454
Fragments arranged by size:
1058 454 276
TatI w'GTAC_w
Cuts at: 0 217 1788
Size: 217 1571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 1173 1788
Size: 363 810 615
Fragments arranged by size:
810 615 363
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI ApoI AvaI BamHI
BanI BanII BbsI BciVI BlpI Bme1580I BmrI BmtI
Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrFI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI Eco57MI EcoRI HaeII Hin4I MslI
MspA1I NcoI NheI NspI PasI PciI PflMI PfoI
PshAI PstI PvuII SexAI SfcI SphI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BfrBI BglI BglII
BmgBI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI