Restriction map for
pF1KB4629
(Linear) (Six-base) MAPSORT of: KIBB4629.seq Check: 3713 from: 1 to: 1323
KIBB4629.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1160 1323
Size: 1160 163
AclI AA'CG_TT
Cuts at: 0 1097 1323
Size: 1097 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 792 954 1323
Size: 544 248 162 369
Fragments arranged by size:
544 369 248 162
AflIII A'CryG_T
Cuts at: 0 1316 1323
Size: 1316 7
AleI CACnn'nnGTG
Cuts at: 0 257 757 1323
Size: 257 500 566
Fragments arranged by size:
566 500 257
ApaLI G'TGCA_C
Cuts at: 0 727 750 856 1323
Size: 727 23 106 467
Fragments arranged by size:
727 467 106 23
ApoI r'AATT_y
Cuts at: 0 997 1144 1323
Size: 997 147 179
Fragments arranged by size:
997 179 147
BanI G'GyrC_C
Cuts at: 0 624 1323
Size: 624 699
BanII G_rGCy'C
Cuts at: 0 78 86 145 779 1323
Size: 78 8 59 634 544
Fragments arranged by size:
634 544 78 59 8
BbeI G_GCGC'C
Cuts at: 0 628 1323
Size: 628 695
BbsI GAAGACnn'nnnn_
Cuts at: 0 726 1270 1323
Size: 726 544 53
Fragments arranged by size:
726 544 53
BfrBI ATG'CAT
Cuts at: 0 268 1323
Size: 268 1055
BglI GCCn_nnn'nGGC
Cuts at: 0 880 1323
Size: 880 443
BglII A'GATC_T
Cuts at: 0 1304 1323
Size: 1304 19
Bme1580I G_kGCm'C
Cuts at: 0 731 754 860 1140 1323
Size: 731 23 106 280 183
Fragments arranged by size:
731 280 183 106 23
BmgBI CAC'GTC
Cuts at: 0 732 1323
Size: 732 591
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 1170 1323
Size: 415 755 153
Fragments arranged by size:
755 415 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1282 1323
Size: 337 945 41
Fragments arranged by size:
945 337 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1323
Size: 829 494
BsaI GGTCTCn'nnnn_
Cuts at: 0 757 1070 1270 1323
Size: 757 313 200 53
Fragments arranged by size:
757 313 200 53
BsaHI Gr'CG_yC
Cuts at: 0 625 1323
Size: 625 698
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 58 135 1323
Size: 55 3 77 1188
Fragments arranged by size:
1188 77 55 3
BseYI C'CCAG_C
Cuts at: 0 802 1323
Size: 802 521
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1323
Size: 1270 53
BsiHKAI G_wGCw'C
Cuts at: 0 86 145 731 754 779 860 1238 1323
Size: 86 59 586 23 25 81 378 85
Fragments arranged by size:
586 378 86 85 81 59 25 23
BsiWI C'GTAC_G
Cuts at: 0 298 1323
Size: 298 1025
Bsp1286I G_dGCh'C
Cuts at: 0 78 86 145 731 754 779 860 1140
Size: 78 8 59 586 23 25 81 280
Cuts at: 1140 1238 1323
Size: 98 85
Fragments arranged by size:
586 280 98 85 81 78 59 25
23 8
BspHI T'CATG_A
Cuts at: 0 1091 1323
Size: 1091 232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1160 1223 1323
Size: 1160 63 100
Fragments arranged by size:
1160 100 63
BsrDI GCAATG_nn'
Cuts at: 0 1052 1323
Size: 1052 271
BsrFI r'CCGG_y
Cuts at: 0 604 1079 1323
Size: 604 475 244
Fragments arranged by size:
604 475 244
BssSI C'ACGA_G
Cuts at: 0 859 1323
Size: 859 464
BstAPI GCAn_nnn'nTGC
Cuts at: 0 371 1293 1323
Size: 371 922 30
Fragments arranged by size:
922 371 30
BstEII G'GTnAC_C
Cuts at: 0 834 1227 1323
Size: 834 393 96
Fragments arranged by size:
834 393 96
BstYI r'GATC_y
Cuts at: 0 1304 1323
Size: 1304 19
Bsu36I CC'TnA_GG
Cuts at: 0 26 1323
Size: 26 1297
BtgI C'CryG_G
Cuts at: 0 1102 1323
Size: 1102 221
BtsI GCAGTG_nn'
Cuts at: 0 157 930 1323
Size: 157 773 393
Fragments arranged by size:
773 393 157
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 906 941 1323
Size: 906 35 382
Fragments arranged by size:
906 382 35
EaeI y'GGCC_r
Cuts at: 0 515 1058 1081 1323
Size: 515 543 23 242
Fragments arranged by size:
543 515 242 23
EarI CTCTTCn'nnn_
Cuts at: 0 32 451 823 1189 1323
Size: 32 419 372 366 134
Fragments arranged by size:
419 372 366 134 32
EcoICRI GAG'CTC
Cuts at: 0 84 143 777 1323
Size: 84 59 634 546
Fragments arranged by size:
634 546 84 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 544 792 954 1282 1323
Size: 337 207 248 162 328 41
Fragments arranged by size:
337 328 248 207 162 41
EcoO109I rG'GnC_Cy
Cuts at: 0 191 1323
Size: 191 1132
EcoRI G'AATT_C
Cuts at: 0 1144 1323
Size: 1144 179
EcoRV GAT'ATC
Cuts at: 0 978 1323
Size: 978 345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 1323
Size: 306 1017
FspI TGC'GCA
Cuts at: 0 952 1323
Size: 952 371
HaeII r_GCGC'y
Cuts at: 0 628 1323
Size: 628 695
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 226 258 1323
Size: 178 32 16 32 1065
Fragments arranged by size:
1065 178 32 32 16
HincII GTy'rAC
Cuts at: 0 894 1323
Size: 894 429
KasI G'GCGC_C
Cuts at: 0 624 1323
Size: 624 699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1323
Size: 418 905
MslI CAynn'nnrTG
Cuts at: 0 257 269 757 1169 1323
Size: 257 12 488 412 154
Fragments arranged by size:
488 412 257 154 12
MspA1I CmG'CkG
Cuts at: 0 341 743 1254 1323
Size: 341 402 511 69
Fragments arranged by size:
511 402 341 69
NarI GG'CG_CC
Cuts at: 0 625 1323
Size: 625 698
NsiI A_TGCA'T
Cuts at: 0 270 1323
Size: 270 1053
NspI r_CATG'y
Cuts at: 0 106 272 414 1320 1323
Size: 106 166 142 906 3
Fragments arranged by size:
906 166 142 106 3
PciI A'CATG_T
Cuts at: 0 1316 1323
Size: 1316 7
PfoI T'CCnGG_A
Cuts at: 0 32 136 1118 1298 1323
Size: 32 104 982 180 25
Fragments arranged by size:
982 180 104 32 25
PstI C_TGCA'G
Cuts at: 0 176 1157 1202 1323
Size: 176 981 45 121
Fragments arranged by size:
981 176 121 45
PvuII CAG'CTG
Cuts at: 0 341 1323
Size: 341 982
SacI G_AGCT'C
Cuts at: 0 86 145 779 1323
Size: 86 59 634 544
Fragments arranged by size:
634 544 86 59
SbfI CC_TGCA'GG
Cuts at: 0 1157 1323
Size: 1157 166
ScaI AGT'ACT
Cuts at: 0 379 1323
Size: 379 944
SexAI A'CCwGG_T
Cuts at: 0 830 1323
Size: 830 493
SfcI C'TryA_G
Cuts at: 0 172 1153 1198 1323
Size: 172 981 45 125
Fragments arranged by size:
981 172 125 45
SfoI GGC'GCC
Cuts at: 0 626 1323
Size: 626 697
SmlI C'TyrA_G
Cuts at: 0 844 1323
Size: 844 479
SphI G_CATG'C
Cuts at: 0 106 1323
Size: 106 1217
StuI AGG'CCT
Cuts at: 0 529 1088 1323
Size: 529 559 235
Fragments arranged by size:
559 529 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1282 1323
Size: 1282 41
TatI w'GTAC_w
Cuts at: 0 377 500 1323
Size: 377 123 823
Fragments arranged by size:
823 377 123
XmnI GAAnn'nnTTC
Cuts at: 0 529 565 1323
Size: 529 36 758
Fragments arranged by size:
758 529 36
Enzymes that do cut:
AarI AclI AcuI AflIII AleI ApaLI ApoI BanI
BanII BbeI BbsI BfrBI BglI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI
BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI
BstAPI BstEII BstYI Bsu36I BtgI BtsI CspCI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI MmeI MslI MspA1I NarI
NsiI NspI PciI PfoI PstI PvuII SacI SbfI
ScaI SexAI SfcI SfoI SmlI SphI StuI TaqII
TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BlpI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiEI BsmI
BsmBI BspEI BsrBI BsrGI BssHII BstBI BstXI BstZ17I
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoNI
FseI FspAI HindIII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI