Restriction map for
pF1KB4636
(Linear) (Six-base) MAPSORT of: KIBB4636.seq Check: 2869 from: 1 to: 3378
KIBB4636.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 771 1052 1443 1992 3378
Size: 771 281 391 549 1386
Fragments arranged by size:
1386 771 549 391 281
Acc65I G'GTAC_C
Cuts at: 0 1576 3378
Size: 1576 1802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1780 2731 2975 3378
Size: 199 1581 951 244 403
Fragments arranged by size:
1581 951 403 244 199
AfeI AGC'GCT
Cuts at: 0 868 3340 3378
Size: 868 2472 38
Fragments arranged by size:
2472 868 38
AleI CACnn'nnGTG
Cuts at: 0 1328 3378
Size: 1328 2050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 155 3157 3378
Size: 155 3002 221
Fragments arranged by size:
3002 221 155
AlwNI CAG_nnn'CTG
Cuts at: 0 837 1549 1684 1812 1846 2908 3178 3378
Size: 837 712 135 128 34 1062 270 200
Fragments arranged by size:
1062 837 712 270 200 135 128 34
ApaI G_GGCC'C
Cuts at: 0 105 566 757 1541 2223 2571 3157 3378
Size: 105 461 191 784 682 348 586 221
Fragments arranged by size:
784 682 586 461 348 221 191 105
ApoI r'AATT_y
Cuts at: 0 2500 2675 3378
Size: 2500 175 703
Fragments arranged by size:
2500 703 175
AvaI C'yCGr_G
Cuts at: 0 350 485 671 1302 1481 2135 2936 2969
Size: 350 135 186 631 179 654 801 33
Cuts at: 2969 3226 3378
Size: 257 152
Fragments arranged by size:
801 654 631 350 257 186 179 152
135 33
BamHI G'GATC_C
Cuts at: 0 334 2185 3378
Size: 334 1851 1193
Fragments arranged by size:
1851 1193 334
BanI G'GyrC_C
Cuts at: 0 1185 1455 1564 1576 1708 3252 3378
Size: 1185 270 109 12 132 1544 126
Fragments arranged by size:
1544 1185 270 132 126 109 12
BanII G_rGCy'C
Cuts at: 0 105 264 485 566 634 680 731 757
Size: 105 159 221 81 68 46 51 26
Cuts at: 757 1263 1541 2125 2136 2223 2378 2571 2968
Size: 506 278 584 11 87 155 193 397
Cuts at: 2968 3157 3210 3226 3378
Size: 189 53 16 152
Fragments arranged by size:
584 506 397 278 221 193 189 159
155 152 105 87 81 68 53 51
46 26 16 11
BbeI G_GCGC'C
Cuts at: 0 1568 3378
Size: 1568 1810
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 1220 3323 3378
Size: 70 1150 2103 55
Fragments arranged by size:
2103 1150 70 55
BbvCI CC'TCA_GC
Cuts at: 0 315 1248 1253 2882 3378
Size: 315 933 5 1629 496
Fragments arranged by size:
1629 933 496 315 5
BclI T'GATC_A
Cuts at: 0 515 947 2414 3378
Size: 515 432 1467 964
Fragments arranged by size:
1467 964 515 432
BglI GCCn_nnn'nGGC
Cuts at: 0 1591 1836 3358 3378
Size: 1591 245 1522 20
Fragments arranged by size:
1591 1522 245 20
BlpI GC'TnA_GC
Cuts at: 0 506 2107 3378
Size: 506 1601 1271
Fragments arranged by size:
1601 1271 506
Bme1580I G_kGCm'C
Cuts at: 0 105 566 711 757 1541 2223 2571 3157
Size: 105 461 145 46 784 682 348 586
Cuts at: 3157 3378
Size: 221
Fragments arranged by size:
784 682 586 461 348 221 145 105
46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1537 2378 3378
Size: 1537 841 1000
Fragments arranged by size:
1537 1000 841
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2982 3378
Size: 2982 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 835 859 1075 1553 1868 2066 2228
Size: 805 30 24 216 478 315 198 162
Cuts at: 2228 2308 2599 2779 3268 3378
Size: 80 291 180 489 110
Fragments arranged by size:
805 489 478 315 291 216 198 180
162 110 80 30 24
Bpu10I CC'TnA_GC
Cuts at: 0 315 1248 1253 1649 2876 2882 3194 3235
Size: 315 933 5 396 1227 6 312 41
Cuts at: 3235 3378
Size: 143
Fragments arranged by size:
1227 933 396 315 312 143 41 6
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 2080 3378
Size: 648 1432 1298
Fragments arranged by size:
1432 1298 648
BsaHI Gr'CG_yC
Cuts at: 0 1565 3378
Size: 1565 1813
BsaWI w'CCGG_w
Cuts at: 0 377 1313 2073 2393 2486 3287 3378
Size: 377 936 760 320 93 801 91
Fragments arranged by size:
936 801 760 377 320 93 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1935 1965 2306 2336 3378
Size: 1935 30 341 30 1042
Fragments arranged by size:
1935 1042 341 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 254 257 572 715 946 1496 1958
Size: 49 205 3 315 143 231 550 462
Cuts at: 1958 2851 3378
Size: 893 527
Fragments arranged by size:
893 550 527 462 315 231 205 143
49 3
BseYI C'CCAG_C
Cuts at: 0 711 741 747 978 1757 2118 2330 2822
Size: 711 30 6 231 779 361 212 492
Cuts at: 2822 2901 3158 3335 3353 3378
Size: 79 257 177 18 25
Fragments arranged by size:
779 711 492 361 257 231 212 177
79 30 25 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 2116 2479 2608 3378
Size: 1011 1105 363 129 770
Fragments arranged by size:
1105 1011 770 363 129
BsiHKAI G_wGCw'C
Cuts at: 0 134 634 731 821 1091 1404 2108 2765
Size: 134 500 97 90 270 313 704 657
Cuts at: 2765 3210 3378
Size: 445 168
Fragments arranged by size:
704 657 500 445 313 270 168 134
97 90
BsmI GAATG_Cn'
Cuts at: 0 387 2611 2898 3378
Size: 387 2224 287 480
Fragments arranged by size:
2224 480 387 287
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3123 3378
Size: 3123 255
Bsp1286I G_dGCh'C
Cuts at: 0 105 134 264 485 566 634 680 711
Size: 105 29 130 221 81 68 46 31
Cuts at: 711 731 757 821 1091 1263 1404 1541 2108
Size: 20 26 64 270 172 141 137 567
Cuts at: 2108 2125 2136 2223 2378 2571 2765 2968 3157
Size: 17 11 87 155 193 194 203 189
Cuts at: 3157 3210 3226 3378
Size: 53 16 152
Fragments arranged by size:
567 270 221 203 194 193 189 172
155 152 141 137 130 105 87 81
68 64 53 46 31 29 26 20
17 16 11
BspEI T'CCGG_A
Cuts at: 0 1313 2393 2486 3378
Size: 1313 1080 93 892
Fragments arranged by size:
1313 1080 892 93
BspHI T'CATG_A
Cuts at: 0 372 1346 3378
Size: 372 974 2032
Fragments arranged by size:
2032 974 372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 771 1016 1052 1443 1829 1992 2451 2630
Size: 771 245 36 391 386 163 459 179
Cuts at: 2630 3378
Size: 748
Fragments arranged by size:
771 748 459 391 386 245 179 163
36
BsrBI CCG'CTC
Cuts at: 0 1607 1741 3378
Size: 1607 134 1637
Fragments arranged by size:
1637 1607 134
BsrDI GCAATG_nn'
Cuts at: 0 134 2525 2544 3378
Size: 134 2391 19 834
Fragments arranged by size:
2391 834 134 19
BsrFI r'CCGG_y
Cuts at: 0 600 1567 3378
Size: 600 967 1811
Fragments arranged by size:
1811 967 600
BssSI C'ACGA_G
Cuts at: 0 919 2690 3378
Size: 919 1771 688
Fragments arranged by size:
1771 919 688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2004 2103 2541 3378
Size: 2004 99 438 837
Fragments arranged by size:
2004 837 438 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1083 1881 2733 3378
Size: 1083 798 852 645
Fragments arranged by size:
1083 852 798 645
BstYI r'GATC_y
Cuts at: 0 334 2185 3378
Size: 334 1851 1193
Fragments arranged by size:
1851 1193 334
Bsu36I CC'TnA_GG
Cuts at: 0 2834 2957 3378
Size: 2834 123 421
Fragments arranged by size:
2834 421 123
BtgI C'CryG_G
Cuts at: 0 680 899 1082 1217 1340 1784 2295 3378
Size: 680 219 183 135 123 444 511 1083
Fragments arranged by size:
1083 680 511 444 219 183 135 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1900 3378
Size: 1900 1478
BtsI GCAGTG_nn'
Cuts at: 0 1555 1688 2154 2625 3378
Size: 1555 133 466 471 753
Fragments arranged by size:
1555 753 471 466 133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1466 1501 3378
Size: 1466 35 1877
Fragments arranged by size:
1877 1466 35
DraIII CAC_nnn'GTG
Cuts at: 0 252 3378
Size: 252 3126
DrdI GACnn_nn'nnGTC
Cuts at: 0 1427 3378
Size: 1427 1951
EaeI y'GGCC_r
Cuts at: 0 1434 2670 3378
Size: 1434 1236 708
Fragments arranged by size:
1434 1236 708
EarI CTCTTCn'nnn_
Cuts at: 0 2255 2423 2918 3378
Size: 2255 168 495 460
Fragments arranged by size:
2255 495 460 168
EcoICRI GAG'CTC
Cuts at: 0 632 729 3208 3378
Size: 632 97 2479 170
Fragments arranged by size:
2479 632 170 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 805 835 859 1075 1553 1780 1868
Size: 199 606 30 24 216 478 227 88
Cuts at: 1868 2066 2228 2308 2599 2731 2779 2975 3268
Size: 198 162 80 291 132 48 196 293
Cuts at: 3268 3378
Size: 110
Fragments arranged by size:
606 478 293 291 227 216 199 198
196 162 132 110 88 80 48 30
24
EcoO109I rG'GnC_Cy
Cuts at: 0 101 355 362 465 562 563 1420 1537
Size: 101 254 7 103 97 1 857 117
Cuts at: 1537 1538 1655 1750 1828 2219 2220 2237 2567
Size: 1 117 95 78 391 1 17 330
Cuts at: 2567 2776 3036 3153 3154 3243 3378
Size: 209 260 117 1 89 135
Fragments arranged by size:
857 391 330 260 254 209 135 117
117 117 103 101 97 95 89 78
17 7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 2675 3378
Size: 2675 703
FspI TGC'GCA
Cuts at: 0 2534 3378
Size: 2534 844
HaeII r_GCGC'y
Cuts at: 0 870 1568 3342 3378
Size: 870 698 1774 36
Fragments arranged by size:
1774 870 698 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 1935 1967 2053 2085 2917 2949
Size: 537 32 1366 32 86 32 832 32
Cuts at: 2949 3378
Size: 429
Fragments arranged by size:
1366 832 537 429 86 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 211 3378
Size: 211 3167
KasI G'GCGC_C
Cuts at: 0 1564 3378
Size: 1564 1814
KpnI G_GTAC'C
Cuts at: 0 1580 3378
Size: 1580 1798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1501 3378
Size: 384 1117 1877
Fragments arranged by size:
1877 1117 384
MscI TGG'CCA
Cuts at: 0 1436 3378
Size: 1436 1942
MslI CAynn'nnrTG
Cuts at: 0 454 1046 1081 1328 1879 2395 2731 3378
Size: 454 592 35 247 551 516 336 647
Fragments arranged by size:
647 592 551 516 454 336 247 35
MspA1I CmG'CkG
Cuts at: 0 28 751 834 942 1252 1732 2334 2886
Size: 28 723 83 108 310 480 602 552
Cuts at: 2886 2995 3016 3069 3162 3357 3378
Size: 109 21 53 93 195 21
Fragments arranged by size:
723 602 552 480 310 195 109 108
93 83 53 28 21 21
NarI GG'CG_CC
Cuts at: 0 1565 3378
Size: 1565 1813
NcoI C'CATG_G
Cuts at: 0 680 1082 1784 2295 3378
Size: 680 402 702 511 1083
Fragments arranged by size:
1083 702 680 511 402
NspI r_CATG'y
Cuts at: 0 1051 2539 2769 3378
Size: 1051 1488 230 609
Fragments arranged by size:
1488 1051 609 230
PasI CC'CwG_GG
Cuts at: 0 1416 1443 2127 3378
Size: 1416 27 684 1251
Fragments arranged by size:
1416 1251 684 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1332 1443 1549 1846 2908 3378
Size: 252 1080 111 106 297 1062 470
Fragments arranged by size:
1080 1062 470 297 252 111 106
PfoI T'CCnGG_A
Cuts at: 0 541 821 1530 2043 3378
Size: 541 280 709 513 1335
Fragments arranged by size:
1335 709 541 513 280
PpuMI rG'GwC_Cy
Cuts at: 0 1420 1655 3036 3243 3378
Size: 1420 235 1381 207 135
Fragments arranged by size:
1420 1381 235 207 135
PspOMI G'GGCC_C
Cuts at: 0 101 562 753 1537 2219 2567 3153 3378
Size: 101 461 191 784 682 348 586 225
Fragments arranged by size:
784 682 586 461 348 225 191 101
PstI C_TGCA'G
Cuts at: 0 1562 1836 3021 3314 3378
Size: 1562 274 1185 293 64
Fragments arranged by size:
1562 1185 293 274 64
PvuII CAG'CTG
Cuts at: 0 1252 1732 2334 2995 3016 3162 3378
Size: 1252 480 602 661 21 146 216
Fragments arranged by size:
1252 661 602 480 216 146 21
SacI G_AGCT'C
Cuts at: 0 634 731 3210 3378
Size: 634 97 2479 168
Fragments arranged by size:
2479 634 168 97
SanDI GG'GwC_CC
Cuts at: 0 1420 1655 3036 3378
Size: 1420 235 1381 342
Fragments arranged by size:
1420 1381 342 235
SbfI CC_TGCA'GG
Cuts at: 0 1836 3378
Size: 1836 1542
SexAI A'CCwGG_T
Cuts at: 0 248 3378
Size: 248 3130
SfcI C'TryA_G
Cuts at: 0 829 1558 1832 2771 3017 3273 3310 3327
Size: 829 729 274 939 246 256 37 17
Cuts at: 3327 3378
Size: 51
Fragments arranged by size:
939 829 729 274 256 246 51 37
17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1836 3378
Size: 1836 1542
SfoI GGC'GCC
Cuts at: 0 1566 3378
Size: 1566 1812
SmaI CCC'GGG
Cuts at: 0 487 673 1483 2971 3378
Size: 487 186 810 1488 407
Fragments arranged by size:
1488 810 487 407 186
SmlI C'TyrA_G
Cuts at: 0 627 2059 3378
Size: 627 1432 1319
Fragments arranged by size:
1432 1319 627
StuI AGG'CCT
Cuts at: 0 1210 2051 2597 3378
Size: 1210 841 546 781
Fragments arranged by size:
1210 841 781 546
StyI C'CwwG_G
Cuts at: 0 68 680 1082 1784 1911 2223 2295 2453
Size: 68 612 402 702 127 312 72 158
Cuts at: 2453 2592 2708 3007 3378
Size: 139 116 299 371
Fragments arranged by size:
702 612 402 371 312 299 158 139
127 116 72 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 798 874 1782 2353 2397 3378
Size: 798 76 908 571 44 981
Fragments arranged by size:
981 908 798 571 76 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1439 1471 3378
Size: 1118 32 289 32 1907
Fragments arranged by size:
1907 1118 289 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 306 3378
Size: 306 3072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 27 1413 1587 1878 3378
Size: 27 1386 174 291 1500
Fragments arranged by size:
1500 1386 291 174 27
XmaI C'CCGG_G
Cuts at: 0 485 671 1481 2969 3378
Size: 485 186 810 1488 409
Fragments arranged by size:
1488 810 485 409 186
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HindIII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SbfI SexAI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BfrBI BglII BmgBI BmtI BsaI BsaAI BsaBI BsiEI
BsiWI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI
EagI EciI EcoNI EcoRV FalI FseI FspAI HincII
HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XhoI XmnI ZraI