Restriction map for
pF1KB4640
(Linear) (Six-base) MAPSORT of: KIBB4640.seq Check: 5493 from: 1 to: 1842
KIBB4640.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1117 1315 1562 1842
Size: 416 701 198 247 280
Fragments arranged by size:
701 416 280 247 198
AflII C'TTAA_G
Cuts at: 0 388 1842
Size: 388 1454
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1721 1753 1842
Size: 1721 32 89
Fragments arranged by size:
1721 89 32
AlwNI CAG_nnn'CTG
Cuts at: 0 728 1842
Size: 728 1114
ApaI G_GGCC'C
Cuts at: 0 530 1842
Size: 530 1312
ApoI r'AATT_y
Cuts at: 0 482 1842
Size: 482 1360
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1023 1056 1842
Size: 1023 33 786
Fragments arranged by size:
1023 786 33
BanII G_rGCy'C
Cuts at: 0 530 1226 1842
Size: 530 696 616
Fragments arranged by size:
696 616 530
BbsI GAAGACnn'nnnn_
Cuts at: 0 1550 1673 1842
Size: 1550 123 169
Fragments arranged by size:
1550 169 123
BbvCI CC'TCA_GC
Cuts at: 0 1645 1842
Size: 1645 197
BclI T'GATC_A
Cuts at: 0 882 1842
Size: 882 960
BglII A'GATC_T
Cuts at: 0 336 1842
Size: 336 1506
BlpI GC'TnA_GC
Cuts at: 0 266 1842
Size: 266 1576
Bme1580I G_kGCm'C
Cuts at: 0 530 1842
Size: 530 1312
BmrI ACTGGGnnnn_n'
Cuts at: 0 382 1623 1842
Size: 382 1241 219
Fragments arranged by size:
1241 382 219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 337 385 556 1682 1842
Size: 15 322 48 171 1126 160
Fragments arranged by size:
1126 322 171 160 48 15
Bpu10I CC'TnA_GC
Cuts at: 0 488 1343 1645 1842
Size: 488 855 302 197
Fragments arranged by size:
855 488 302 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1842
Size: 769 1073
BsaI GGTCTCn'nnnn_
Cuts at: 0 470 1842
Size: 470 1372
BsaWI w'CCGG_w
Cuts at: 0 580 1842
Size: 580 1262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1264 1294 1842
Size: 1264 30 548
Fragments arranged by size:
1264 548 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 772 1216 1820 1842
Size: 259 513 444 604 22
Fragments arranged by size:
604 513 444 259 22
BseYI C'CCAG_C
Cuts at: 0 658 1144 1842
Size: 658 486 698
Fragments arranged by size:
698 658 486
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1212 1230 1842
Size: 1212 18 612
Fragments arranged by size:
1212 612 18
BsiHKAI G_wGCw'C
Cuts at: 0 494 1842
Size: 494 1348
Bsp1286I G_dGCh'C
Cuts at: 0 494 530 1226 1842
Size: 494 36 696 616
Fragments arranged by size:
696 616 494 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 636 1842
Size: 636 1206
BsrDI GCAATG_nn'
Cuts at: 0 1433 1842
Size: 1433 409
BstBI TT'CG_AA
Cuts at: 0 1683 1842
Size: 1683 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 852 1071 1842
Size: 852 219 771
Fragments arranged by size:
852 771 219
BstYI r'GATC_y
Cuts at: 0 336 1842
Size: 336 1506
DraI TTT'AAA
Cuts at: 0 1569 1842
Size: 1569 273
EarI CTCTTCn'nnn_
Cuts at: 0 535 1842
Size: 535 1307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 337 385 416 556 1117 1315 1562
Size: 15 322 48 31 140 561 198 247
Cuts at: 1562 1682 1842
Size: 120 160
Fragments arranged by size:
561 322 247 198 160 140 120 48
31 15
EcoO109I rG'GnC_Cy
Cuts at: 0 527 1185 1499 1617 1842
Size: 527 658 314 118 225
Fragments arranged by size:
658 527 314 225 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 714 1723 1842
Size: 714 1009 119
Fragments arranged by size:
1009 714 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 536 556 568 588 1842
Size: 536 20 12 20 1254
Fragments arranged by size:
1254 536 20 20 12
HindIII A'AGCT_T
Cuts at: 0 1233 1842
Size: 1233 609
MfeI C'AATT_G
Cuts at: 0 297 1842
Size: 297 1545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1463 1842
Size: 843 620 379
Fragments arranged by size:
843 620 379
MslI CAynn'nnrTG
Cuts at: 0 850 1842
Size: 850 992
MspA1I CmG'CkG
Cuts at: 0 1609 1842
Size: 1609 233
PfoI T'CCnGG_A
Cuts at: 0 533 1842
Size: 533 1309
PpuMI rG'GwC_Cy
Cuts at: 0 1185 1617 1842
Size: 1185 432 225
Fragments arranged by size:
1185 432 225
PspOMI G'GGCC_C
Cuts at: 0 526 1842
Size: 526 1316
PstI C_TGCA'G
Cuts at: 0 1767 1842
Size: 1767 75
PvuII CAG'CTG
Cuts at: 0 1609 1842
Size: 1609 233
SfcI C'TryA_G
Cuts at: 0 1720 1763 1834 1842
Size: 1720 43 71 8
Fragments arranged by size:
1720 71 43 8
SmlI C'TyrA_G
Cuts at: 0 388 784 1842
Size: 388 396 1058
Fragments arranged by size:
1058 396 388
SpeI A'CTAG_T
Cuts at: 0 832 1842
Size: 832 1010
SspI AAT'ATT
Cuts at: 0 522 1842
Size: 522 1320
StyI C'CwwG_G
Cuts at: 0 615 698 1523 1842
Size: 615 83 825 319
Fragments arranged by size:
825 615 319 83
Tth111I GACn'n_nGTC
Cuts at: 0 1615 1842
Size: 1615 227
XbaI T'CTAG_A
Cuts at: 0 1438 1842
Size: 1438 404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 19 1146 1657 1842
Size: 19 1127 511 185
Fragments arranged by size:
1127 511 185 19
XmnI GAAnn'nnTTC
Cuts at: 0 7 1842
Size: 7 1835
Enzymes that do cut:
AcuI AflII AloI AlwNI ApaI ApoI BaeI BanII
BbsI BbvCI BclI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BstBI BstXI BstYI DraI
EarI Eco57MI EcoO109I FalI Hin4I HindIII MfeI MmeI
MslI MspA1I PfoI PpuMI PspOMI PstI PvuII SfcI
SmlI SpeI SspI StyI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanI BbeI BcgI BciVI BfrBI BglI
BmgBI BmtI BplI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI SwaI TaqII TatI
TstI XhoI XmaI ZraI