(Linear) (Six-base) MAPSORT of: KIBB4653.seq  Check: 3975  from: 1  to: 600



KIBB4653.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     600 
   Size:        521      79
 
BanII G_rGCy'C

Cuts at:      0     536     600 
   Size:        536      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      16      70     600 
   Size:         16      54     530

  Fragments arranged by size: 

                 530      54      16
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     183     600 
   Size:        183     417
 
BsaWI w'CCGG_w

Cuts at:      0      52     600 
   Size:         52     548
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     557     587     600 
   Size:        557      30      13

  Fragments arranged by size: 

                 557      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     581     600 
   Size:        581      19
 
BsiWI C'GTAC_G

Cuts at:      0      14     600 
   Size:         14     586
 
Bsp1286I G_dGCh'C

Cuts at:      0     536     600 
   Size:        536      64
 
BspEI T'CCGG_A

Cuts at:      0      52     600 
   Size:         52     548
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      29      76     600 
   Size:         29      47     524

  Fragments arranged by size: 

                 524      47      29
 
BsrDI GCAATG_nn'

Cuts at:      0     251     600 
   Size:        251     349
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     596     600 
   Size:        596       4
 
BstBI TT'CG_AA

Cuts at:      0     513     600 
   Size:        513      87
 
BtsI GCAGTG_nn'

Cuts at:      0     567     600 
   Size:        567      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     580     600 
   Size:        580      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     600 
   Size:        521      79
 
EcoRV GAT'ATC

Cuts at:      0     507     555     600 
   Size:        507      48      45

  Fragments arranged by size: 

                 507      48      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     526     600 
   Size:        526      74
 
MslI CAynn'nnrTG

Cuts at:      0     332     600 
   Size:        332     268
 
NspI r_CATG'y

Cuts at:      0     331     600 
   Size:        331     269
 
PshAI GACnn'nnGTC

Cuts at:      0      70     600 
   Size:         70     530
 
SapI GCTCTTCn'nnn_

Cuts at:      0     580     600 
   Size:        580      20
 
SfcI C'TryA_G

Cuts at:      0     178     234     600 
   Size:        178      56     366

  Fragments arranged by size: 

                 366     178      56
 
SpeI A'CTAG_T

Cuts at:      0     451     600 
   Size:        451     149
 
SphI G_CATG'C

Cuts at:      0     331     600 
   Size:        331     269
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     557     589     600 
   Size:        557      32      11

  Fragments arranged by size: 

                 557      32      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     339     600 
   Size:        339     261
 

 Enzymes that do cut:

     AcuI    BanII     BbsI    BciVI    BsaWI    BsaXI    BseRI    BsiWI
 Bsp1286I    BspEI    BspMI    BsrDI   BstAPI    BstBI     BtsI     EarI
  Eco57MI    EcoRV     FalI     MslI     NspI    PshAI     SapI     SfcI
     SpeI     SphI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI