Restriction map for
pF1KB4658
(Linear) (Six-base) MAPSORT of: KIBB4658.seq Check: 7955 from: 1 to: 2418
KIBB4658.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1445 2418
Size: 1445 973
AatII G_ACGT'C
Cuts at: 0 1730 2418
Size: 1730 688
AccI GT'mk_AC
Cuts at: 0 2360 2418
Size: 2360 58
AclI AA'CG_TT
Cuts at: 0 1847 2418
Size: 1847 571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 1711 2256 2418
Size: 15 1696 545 162
Fragments arranged by size:
1696 545 162 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 481 1295 2418
Size: 481 814 1123
Fragments arranged by size:
1123 814 481
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1627 1659 2418
Size: 1627 32 759
Fragments arranged by size:
1627 759 32
AlwNI CAG_nnn'CTG
Cuts at: 0 303 327 603 631 1819 2022 2406 2418
Size: 303 24 276 28 1188 203 384 12
Fragments arranged by size:
1188 384 303 276 203 28 24 12
ApaI G_GGCC'C
Cuts at: 0 751 1380 1676 2418
Size: 751 629 296 742
Fragments arranged by size:
751 742 629 296
ApoI r'AATT_y
Cuts at: 0 246 2418
Size: 246 2172
AvaI C'yCGr_G
Cuts at: 0 99 1509 2418
Size: 99 1410 909
Fragments arranged by size:
1410 909 99
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 399 432 2418
Size: 399 33 1986
Fragments arranged by size:
1986 399 33
BamHI G'GATC_C
Cuts at: 0 389 2208 2418
Size: 389 1819 210
Fragments arranged by size:
1819 389 210
BanI G'GyrC_C
Cuts at: 0 1549 2418
Size: 1549 869
BanII G_rGCy'C
Cuts at: 0 292 751 1279 1317 1380 1405 1654 1676
Size: 292 459 528 38 63 25 249 22
Cuts at: 1676 2418
Size: 742
Fragments arranged by size:
742 528 459 292 249 63 38 25
22
BbsI GAAGACnn'nnnn_
Cuts at: 0 558 2115 2418
Size: 558 1557 303
Fragments arranged by size:
1557 558 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 969 1003 2418
Size: 969 34 1415
Fragments arranged by size:
1415 969 34
BclI T'GATC_A
Cuts at: 0 809 1094 2418
Size: 809 285 1324
Fragments arranged by size:
1324 809 285
BglI GCCn_nnn'nGGC
Cuts at: 0 373 755 2418
Size: 373 382 1663
Fragments arranged by size:
1663 382 373
BlpI GC'TnA_GC
Cuts at: 0 1316 1868 2418
Size: 1316 552 550
Fragments arranged by size:
1316 552 550
Bme1580I G_kGCm'C
Cuts at: 0 751 1380 1552 1676 2238 2418
Size: 751 629 172 124 562 180
Fragments arranged by size:
751 629 562 180 172 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 885 1548 2188 2418
Size: 885 663 640 230
Fragments arranged by size:
885 663 640 230
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 311 1293 1524 2418
Size: 311 982 231 894
Fragments arranged by size:
982 894 311 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1580 1638 1699 1796 1847 2418
Size: 241 1339 58 61 97 51 571
Fragments arranged by size:
1339 571 241 97 61 58 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 553 2344 2418
Size: 553 1791 74
Fragments arranged by size:
1791 553 74
BsaAI yAC'GTr
Cuts at: 0 966 2418
Size: 966 1452
BsaBI GATnn'nnATC
Cuts at: 0 811 2418
Size: 811 1607
BsaHI Gr'CG_yC
Cuts at: 0 38 236 1727 2418
Size: 38 198 1491 691
Fragments arranged by size:
1491 691 198 38
BsaWI w'CCGG_w
Cuts at: 0 1796 2418
Size: 1796 622
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 548 578 1109 1139 1445 1475 2418
Size: 548 30 531 30 306 30 943
Fragments arranged by size:
943 548 531 306 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 463 1469 1472 1477 1696 2395 2418
Size: 463 1006 3 5 219 699 23
Fragments arranged by size:
1006 699 463 219 23 5 3
BseYI C'CCAG_C
Cuts at: 0 266 352 687 2131 2285 2289 2418
Size: 266 86 335 1444 154 4 129
Fragments arranged by size:
1444 335 266 154 129 86 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 441 451 535 655 946 1045 1570
Size: 289 152 10 84 120 291 99 525
Cuts at: 1570 2418
Size: 848
Fragments arranged by size:
848 525 291 289 152 120 99 84
10
BsiHKAI G_wGCw'C
Cuts at: 0 292 1046 1317 1712 2375 2418
Size: 292 754 271 395 663 43
Fragments arranged by size:
754 663 395 292 271 43
Bsp1286I G_dGCh'C
Cuts at: 0 292 751 1046 1279 1317 1380 1405 1552
Size: 292 459 295 233 38 63 25 147
Cuts at: 1552 1654 1676 1712 2238 2375 2418
Size: 102 22 36 526 137 43
Fragments arranged by size:
526 459 295 292 233 147 137 102
63 43 38 36 25 22
BspEI T'CCGG_A
Cuts at: 0 1796 2418
Size: 1796 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1445 2418
Size: 1445 973
BsrDI GCAATG_nn'
Cuts at: 0 732 2418
Size: 732 1686
BsrFI r'CCGG_y
Cuts at: 0 1769 1776 2418
Size: 1769 7 642
Fragments arranged by size:
1769 642 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2178 2418
Size: 2178 240
BstYI r'GATC_y
Cuts at: 0 389 497 833 1356 2000 2208 2418
Size: 389 108 336 523 644 208 210
Fragments arranged by size:
644 523 389 336 210 208 108
Bsu36I CC'TnA_GG
Cuts at: 0 296 1855 2418
Size: 296 1559 563
Fragments arranged by size:
1559 563 296
BtgI C'CryG_G
Cuts at: 0 944 1014 1035 1223 1254 1265 1598 2418
Size: 944 70 21 188 31 11 333 820
Fragments arranged by size:
944 820 333 188 70 31 21 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1476 2418
Size: 1476 942
BtsI GCAGTG_nn'
Cuts at: 0 632 2418
Size: 632 1786
DraIII CAC_nnn'GTG
Cuts at: 0 199 2418
Size: 199 2219
DrdI GACnn_nn'nnGTC
Cuts at: 0 2088 2418
Size: 2088 330
EaeI y'GGCC_r
Cuts at: 0 12 86 773 1067 1517 1577 1967 2150
Size: 12 74 687 294 450 60 390 183
Cuts at: 2150 2418
Size: 268
Fragments arranged by size:
687 450 390 294 268 183 74 60
12
EarI CTCTTCn'nnn_
Cuts at: 0 217 1798 2418
Size: 217 1581 620
Fragments arranged by size:
1581 620 217
EcoICRI GAG'CTC
Cuts at: 0 290 1315 2418
Size: 290 1025 1103
Fragments arranged by size:
1103 1025 290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 241 1580 1638 1699 1711 1796 1847
Size: 15 226 1339 58 61 12 85 51
Cuts at: 1847 2256 2418
Size: 409 162
Fragments arranged by size:
1339 409 226 162 85 61 58 51
15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 640 2418
Size: 640 1778
EcoO109I rG'GnC_Cy
Cuts at: 0 300 315 748 1101 1260 1527 1658 1672
Size: 300 15 433 353 159 267 131 14
Cuts at: 1672 1673 2019 2090 2418
Size: 1 346 71 328
Fragments arranged by size:
433 353 346 328 300 267 159 131
71 15 14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1221 2418
Size: 1221 1197
FspI TGC'GCA
Cuts at: 0 979 2418
Size: 979 1439
HaeII r_GCGC'y
Cuts at: 0 1288 1947 2418
Size: 1288 659 471
Fragments arranged by size:
1288 659 471
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 194 226 548 580 1194 1226
Size: 82 32 80 32 322 32 614 32
Cuts at: 1226 2418
Size: 1192
Fragments arranged by size:
1192 614 322 82 80 32 32 32
32
HincII GTy'rAC
Cuts at: 0 180 1851 2337 2418
Size: 180 1671 486 81
Fragments arranged by size:
1671 486 180 81
HpaI GTT'AAC
Cuts at: 0 1851 2418
Size: 1851 567
MfeI C'AATT_G
Cuts at: 0 432 2418
Size: 432 1986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 231 1956 2418
Size: 206 25 1725 462
Fragments arranged by size:
1725 462 206 25
MscI TGG'CCA
Cuts at: 0 1069 1519 1579 1969 2152 2418
Size: 1069 450 60 390 183 266
Fragments arranged by size:
1069 450 390 266 183 60
MslI CAynn'nnrTG
Cuts at: 0 532 817 1717 2418
Size: 532 285 900 701
Fragments arranged by size:
900 701 532 285
MspA1I CmG'CkG
Cuts at: 0 129 219 270 356 425 669 687 946
Size: 129 90 51 86 69 244 18 259
Cuts at: 946 1037 1059 1267 1884 1910 2289 2403 2418
Size: 91 22 208 617 26 379 114 15
Fragments arranged by size:
617 379 259 244 208 129 114 91
90 86 69 51 26 22 18 15
NaeI GCC'GGC
Cuts at: 0 1778 2418
Size: 1778 640
NcoI C'CATG_G
Cuts at: 0 1598 2418
Size: 1598 820
NgoMIV G'CCGG_C
Cuts at: 0 1776 2418
Size: 1776 642
NspI r_CATG'y
Cuts at: 0 906 1611 1620 2418
Size: 906 705 9 798
Fragments arranged by size:
906 798 705 9
PasI CC'CwG_GG
Cuts at: 0 2076 2141 2418
Size: 2076 65 277
Fragments arranged by size:
2076 277 65
PfoI T'CCnGG_A
Cuts at: 0 1823 1949 2418
Size: 1823 126 469
Fragments arranged by size:
1823 469 126
PmlI CAC'GTG
Cuts at: 0 966 2418
Size: 966 1452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1775 1807 2418
Size: 1775 32 611
Fragments arranged by size:
1775 611 32
PpuMI rG'GwC_Cy
Cuts at: 0 300 315 1260 1527 2019 2090 2418
Size: 300 15 945 267 492 71 328
Fragments arranged by size:
945 492 328 300 267 71 15
PshAI GACnn'nnGTC
Cuts at: 0 1726 2356 2418
Size: 1726 630 62
Fragments arranged by size:
1726 630 62
PspOMI G'GGCC_C
Cuts at: 0 747 1376 1672 2418
Size: 747 629 296 746
Fragments arranged by size:
747 746 629 296
PspXI vC'TCGA_Gb
Cuts at: 0 99 2418
Size: 99 2319
PstI C_TGCA'G
Cuts at: 0 323 608 1284 2418
Size: 323 285 676 1134
Fragments arranged by size:
1134 676 323 285
PvuII CAG'CTG
Cuts at: 0 129 219 270 356 425 687 1884 2289
Size: 129 90 51 86 69 262 1197 405
Cuts at: 2289 2403 2418
Size: 114 15
Fragments arranged by size:
1197 405 262 129 114 90 86 69
51 15
SacI G_AGCT'C
Cuts at: 0 292 1317 2418
Size: 292 1025 1101
Fragments arranged by size:
1101 1025 292
SacII CC_GC'GG
Cuts at: 0 947 1038 1268 2418
Size: 947 91 230 1150
Fragments arranged by size:
1150 947 230 91
SanDI GG'GwC_CC
Cuts at: 0 1260 2418
Size: 1260 1158
ScaI AGT'ACT
Cuts at: 0 520 2269 2418
Size: 520 1749 149
Fragments arranged by size:
1749 520 149
SexAI A'CCwGG_T
Cuts at: 0 641 2418
Size: 641 1777
SfcI C'TryA_G
Cuts at: 0 319 604 1280 1351 2418
Size: 319 285 676 71 1067
Fragments arranged by size:
1067 676 319 285 71
SmlI C'TyrA_G
Cuts at: 0 99 2418
Size: 99 2319
SphI G_CATG'C
Cuts at: 0 906 2418
Size: 906 1512
StuI AGG'CCT
Cuts at: 0 1469 1507 1860 2418
Size: 1469 38 353 558
Fragments arranged by size:
1469 558 353 38
StyI C'CwwG_G
Cuts at: 0 612 654 1523 1598 1739 2171 2418
Size: 612 42 869 75 141 432 247
Fragments arranged by size:
869 612 432 247 141 75 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 122 346 1263 1530 2011 2368 2418
Size: 122 224 917 267 481 357 50
Fragments arranged by size:
917 481 357 267 224 122 50
TatI w'GTAC_w
Cuts at: 0 518 1619 2009 2267 2418
Size: 518 1101 390 258 151
Fragments arranged by size:
1101 518 390 258 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1109 1141 1445 1477 1579 1611 2418
Size: 1109 32 304 32 102 32 807
Fragments arranged by size:
1109 807 304 102 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 169 1761 2418
Size: 169 1592 657
Fragments arranged by size:
1592 657 169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 2418
Size: 360 2058
XhoI C'TCGA_G
Cuts at: 0 99 2418
Size: 99 2319
ZraI GAC'GTC
Cuts at: 0 1728 2418
Size: 1728 690
Enzymes that do cut:
AarI AatII AccI AclI AcuI AlfI AloI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BbsI
BcgI BclI BglI BlpI Bme1580I BmrI BplI BpmI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII HpaI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SanDI ScaI SexAI SfcI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AgeI AhdI AleI ApaLI
AscI AseI AsiSI AvrII BbeI BbvCI BciVI BfrBI
BglII BmgBI BmtI Bpu10I BpuEI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraI EagI EciI EcoRI
EcoRV FseI FspAI HindIII KasI KpnI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PsiI PsrI PvuI RsrII SalI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XmaI XmnI