Restriction map for
pF1KB4659
(Linear) (Six-base) MAPSORT of: KIBB4659.seq Check: 1347 from: 1 to: 1440
KIBB4659.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 835 1440
Size: 835 605
AccI GT'mk_AC
Cuts at: 0 227 1440
Size: 227 1213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1440
Size: 565 875
AleI CACnn'nnGTG
Cuts at: 0 1145 1159 1440
Size: 1145 14 281
Fragments arranged by size:
1145 281 14
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1440
Size: 1081 32 327
Fragments arranged by size:
1081 327 32
AlwNI CAG_nnn'CTG
Cuts at: 0 192 278 329 591 801 1086 1248 1440
Size: 192 86 51 262 210 285 162 192
Fragments arranged by size:
285 262 210 192 192 162 86 51
ApaI G_GGCC'C
Cuts at: 0 82 131 456 1017 1440
Size: 82 49 325 561 423
Fragments arranged by size:
561 423 325 82 49
AvaI C'yCGr_G
Cuts at: 0 57 75 122 181 457 1440
Size: 57 18 47 59 276 983
Fragments arranged by size:
983 276 59 57 47 18
BanI G'GyrC_C
Cuts at: 0 281 332 687 996 1440
Size: 281 51 355 309 444
Fragments arranged by size:
444 355 309 281 51
BanII G_rGCy'C
Cuts at: 0 35 82 111 131 456 581 917 993
Size: 35 47 29 20 325 125 336 76
Cuts at: 993 1017 1440
Size: 24 423
Fragments arranged by size:
423 336 325 125 76 47 35 29
24 20
BbeI G_GCGC'C
Cuts at: 0 285 336 1440
Size: 285 51 1104
Fragments arranged by size:
1104 285 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 553 956 1040 1306 1440
Size: 553 403 84 266 134
Fragments arranged by size:
553 403 266 134 84
BbvCI CC'TCA_GC
Cuts at: 0 897 1440
Size: 897 543
BglI GCCn_nnn'nGGC
Cuts at: 0 352 685 1440
Size: 352 333 755
Fragments arranged by size:
755 352 333
Bme1580I G_kGCm'C
Cuts at: 0 82 131 456 1017 1440
Size: 82 49 325 561 423
Fragments arranged by size:
561 423 325 82 49
BmgBI CAC'GTC
Cuts at: 0 771 1440
Size: 771 669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 125 1007 1440
Size: 125 882 433
Fragments arranged by size:
882 433 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 886 931 1270 1350 1399 1440
Size: 220 666 45 339 80 49 41
Fragments arranged by size:
666 339 220 80 49 45 41
Bpu10I CC'TnA_GC
Cuts at: 0 897 992 1440
Size: 897 95 448
Fragments arranged by size:
897 448 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 1246 1440
Size: 19 1227 194
Fragments arranged by size:
1227 194 19
BsaAI yAC'GTr
Cuts at: 0 1362 1440
Size: 1362 78
BsaHI Gr'CG_yC
Cuts at: 0 65 282 333 832 1440
Size: 65 217 51 499 608
Fragments arranged by size:
608 499 217 65 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 604 634 1440
Size: 604 30 806
Fragments arranged by size:
806 604 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 166 612 856 1440
Size: 103 63 446 244 584
Fragments arranged by size:
584 446 244 103 63
BseYI C'CCAG_C
Cuts at: 0 130 438 1440
Size: 130 308 1002
Fragments arranged by size:
1002 308 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 271 286 1440
Size: 238 33 15 1154
Fragments arranged by size:
1154 238 33 15
BsiHKAI G_wGCw'C
Cuts at: 0 35 560 581 1166 1440
Size: 35 525 21 585 274
Fragments arranged by size:
585 525 274 35 21
Bsp1286I G_dGCh'C
Cuts at: 0 35 82 111 131 456 560 581 917
Size: 35 47 29 20 325 104 21 336
Cuts at: 917 993 1017 1166 1440
Size: 76 24 149 274
Fragments arranged by size:
336 325 274 149 104 76 47 35
29 24 21 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 302 1440
Size: 53 249 1138
Fragments arranged by size:
1138 249 53
BsrBI CCG'CTC
Cuts at: 0 1254 1399 1440
Size: 1254 145 41
Fragments arranged by size:
1254 145 41
BsrDI GCAATG_nn'
Cuts at: 0 341 1440
Size: 341 1099
BsrFI r'CCGG_y
Cuts at: 0 550 1440
Size: 550 890
BsrGI T'GTAC_A
Cuts at: 0 1316 1440
Size: 1316 124
BssHII G'CGCG_C
Cuts at: 0 1384 1440
Size: 1384 56
BstAPI GCAn_nnn'nTGC
Cuts at: 0 294 801 1440
Size: 294 507 639
Fragments arranged by size:
639 507 294
BstEII G'GTnAC_C
Cuts at: 0 421 888 1440
Size: 421 467 552
Fragments arranged by size:
552 467 421
Bsu36I CC'TnA_GG
Cuts at: 0 653 1070 1440
Size: 653 417 370
Fragments arranged by size:
653 417 370
BtgI C'CryG_G
Cuts at: 0 146 508 1038 1440
Size: 146 362 530 402
Fragments arranged by size:
530 402 362 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1116 1440
Size: 1116 324
BtsI GCAGTG_nn'
Cuts at: 0 245 606 1191 1440
Size: 245 361 585 249
Fragments arranged by size:
585 361 249 245
EaeI y'GGCC_r
Cuts at: 0 320 398 505 572 1440
Size: 320 78 107 67 868
Fragments arranged by size:
868 320 107 78 67
EarI CTCTTCn'nnn_
Cuts at: 0 1330 1440
Size: 1330 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 1330 1440
Size: 25 1305 110
Fragments arranged by size:
1305 110 25
EcoICRI GAG'CTC
Cuts at: 0 33 579 1440
Size: 33 546 861
Fragments arranged by size:
861 546 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 565 886 931 1270 1350 1399 1440
Size: 220 345 321 45 339 80 49 41
Fragments arranged by size:
345 339 321 220 80 49 45 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 652 1440
Size: 163 489 788
Fragments arranged by size:
788 489 163
EcoO109I rG'GnC_Cy
Cuts at: 0 79 118 127 156 453 729 1440
Size: 79 39 9 29 297 276 711
Fragments arranged by size:
711 297 276 79 39 29 9
FspI TGC'GCA
Cuts at: 0 469 1440
Size: 469 971
HaeII r_GCGC'y
Cuts at: 0 137 285 336 781 1440
Size: 137 148 51 445 659
Fragments arranged by size:
659 445 148 137 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 1440
Size: 817 32 591
Fragments arranged by size:
817 591 32
KasI G'GCGC_C
Cuts at: 0 281 332 1440
Size: 281 51 1108
Fragments arranged by size:
1108 281 51
MscI TGG'CCA
Cuts at: 0 322 1440
Size: 322 1118
MslI CAynn'nnrTG
Cuts at: 0 145 308 1135 1145 1159 1440
Size: 145 163 827 10 14 281
Fragments arranged by size:
827 281 163 145 14 10
MspA1I CmG'CkG
Cuts at: 0 801 901 1059 1083 1440
Size: 801 100 158 24 357
Fragments arranged by size:
801 357 158 100 24
NarI GG'CG_CC
Cuts at: 0 282 333 1440
Size: 282 51 1107
Fragments arranged by size:
1107 282 51
NcoI C'CATG_G
Cuts at: 0 508 1038 1440
Size: 508 530 402
Fragments arranged by size:
530 508 402
NspI r_CATG'y
Cuts at: 0 795 936 1392 1440
Size: 795 141 456 48
Fragments arranged by size:
795 456 141 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 278 329 1248 1440
Size: 278 51 919 192
Fragments arranged by size:
919 278 192 51
PfoI T'CCnGG_A
Cuts at: 0 908 1247 1376 1440
Size: 908 339 129 64
Fragments arranged by size:
908 339 129 64
PmlI CAC'GTG
Cuts at: 0 1362 1440
Size: 1362 78
PpuMI rG'GwC_Cy
Cuts at: 0 156 729 1440
Size: 156 573 711
Fragments arranged by size:
711 573 156
PspOMI G'GGCC_C
Cuts at: 0 78 127 452 1013 1440
Size: 78 49 325 561 427
Fragments arranged by size:
561 427 325 78 49
PspXI vC'TCGA_Gb
Cuts at: 0 122 181 1440
Size: 122 59 1259
Fragments arranged by size:
1259 122 59
PstI C_TGCA'G
Cuts at: 0 413 806 1440
Size: 413 393 634
Fragments arranged by size:
634 413 393
PvuII CAG'CTG
Cuts at: 0 801 901 1083 1440
Size: 801 100 182 357
Fragments arranged by size:
801 357 182 100
SacI G_AGCT'C
Cuts at: 0 35 581 1440
Size: 35 546 859
Fragments arranged by size:
859 546 35
SanDI GG'GwC_CC
Cuts at: 0 729 1440
Size: 729 711
SexAI A'CCwGG_T
Cuts at: 0 380 1440
Size: 380 1060
SfcI C'TryA_G
Cuts at: 0 409 802 1110 1440
Size: 409 393 308 330
Fragments arranged by size:
409 393 330 308
SfoI GGC'GCC
Cuts at: 0 283 334 1440
Size: 283 51 1106
Fragments arranged by size:
1106 283 51
SmaI CCC'GGG
Cuts at: 0 59 1440
Size: 59 1381
SmlI C'TyrA_G
Cuts at: 0 34 122 181 1261 1440
Size: 34 88 59 1080 179
Fragments arranged by size:
1080 179 88 59 34
SphI G_CATG'C
Cuts at: 0 795 1392 1440
Size: 795 597 48
Fragments arranged by size:
795 597 48
StuI AGG'CCT
Cuts at: 0 538 1440
Size: 538 902
StyI C'CwwG_G
Cuts at: 0 317 508 521 1038 1440
Size: 317 191 13 517 402
Fragments arranged by size:
517 402 317 191 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 619 941 1440
Size: 619 322 499
Fragments arranged by size:
619 499 322
TatI w'GTAC_w
Cuts at: 0 1316 1440
Size: 1316 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4 36 297 329 550 582 604 636
Size: 4 32 261 32 221 32 22 32
Cuts at: 636 1440
Size: 804
Fragments arranged by size:
804 261 221 32 32 32 32 22
4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1198 1440
Size: 1198 242
XhoI C'TCGA_G
Cuts at: 0 122 181 1440
Size: 122 59 1259
Fragments arranged by size:
1259 122 59
XmaI C'CCGG_G
Cuts at: 0 57 1440
Size: 57 1383
ZraI GAC'GTC
Cuts at: 0 833 1440
Size: 833 607
Enzymes that do cut:
AatII AccI AcuI AleI AloI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstEII Bsu36I BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI
NspI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII SacI SanDI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BsaI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BssSI BstBI BstXI BstYI BstZ17I ClaI
CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PpiI PshAI PsiI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XmnI