(Linear) (Six-base) MAPSORT of: KIBB4662.seq  Check: 1120  from: 1  to: 1182



KIBB4662.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AccI GT'mk_AC

Cuts at:      0     470     919    1182 
   Size:        470     449     263

  Fragments arranged by size: 

                 470     449     263
 
AleI CACnn'nnGTG

Cuts at:      0     136    1182 
   Size:        136    1046
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      77     109    1182 
   Size:         77      32    1073

  Fragments arranged by size: 

                1073      77      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     696    1182 
   Size:        696     486
 
ApoI r'AATT_y

Cuts at:      0     886    1015    1182 
   Size:        886     129     167

  Fragments arranged by size: 

                 886     167     129
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1114    1147    1182 
   Size:       1114      33      35

  Fragments arranged by size: 

                1114      35      33
 
BanI G'GyrC_C

Cuts at:      0      40    1182 
   Size:         40    1142
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     117    1040    1182 
   Size:        117     923     142

  Fragments arranged by size: 

                 923     142     117
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     595     938    1182 
   Size:        595     343     244

  Fragments arranged by size: 

                 595     343     244
 
BclI T'GATC_A

Cuts at:      0     809    1182 
   Size:        809     373
 
BglI GCCn_nnn'nGGC

Cuts at:      0     421    1182 
   Size:        421     761
 
BglII A'GATC_T

Cuts at:      0     880    1182 
   Size:        880     302
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     496    1182 
   Size:        496     686
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     932    1182 
   Size:        497     435     250

  Fragments arranged by size: 

                 497     435     250
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    1182 
   Size:        428     754
 
BsaAI yAC'GTr

Cuts at:      0     666    1182 
   Size:        666     516
 
BsaWI w'CCGG_w

Cuts at:      0     400    1182 
   Size:        400     782
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     318     348     470     500     815     845    1182 
   Size:        318      30     122      30     315      30     337

  Fragments arranged by size: 

                 337     318     315     122      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0      96     553    1182 
   Size:         96     457     629

  Fragments arranged by size: 

                 629     457      96
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1094    1182 
   Size:       1094      88
 
BspHI T'CATG_A

Cuts at:      0    1091    1182 
   Size:       1091      91
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     138     543    1182 
   Size:        138     405     639

  Fragments arranged by size: 

                 639     405     138
 
BstYI r'GATC_y

Cuts at:      0     159     880    1022    1182 
   Size:        159     721     142     160

  Fragments arranged by size: 

                 721     160     159     142
 
DraIII CAC_nnn'GTG

Cuts at:      0     903    1182 
   Size:        903     279
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     932    1182 
   Size:        497     435     250

  Fragments arranged by size: 

                 497     435     250
 
EcoRI G'AATT_C

Cuts at:      0     886    1182 
   Size:        886     296
 
EcoRV GAT'ATC

Cuts at:      0     574    1089    1182 
   Size:        574     515      93

  Fragments arranged by size: 

                 574     515      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     563     595     737     769     865     897 
   Size:        104      32     427      32     142      32      96      32

Cuts at:    897    1182 
   Size:        285

  Fragments arranged by size: 

                 427     285     142     104      96      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     210    1182 
   Size:        210     972
 
HindIII A'AGCT_T

Cuts at:      0     788    1182 
   Size:        788     394
 
HpaI GTT'AAC

Cuts at:      0     210    1182 
   Size:        210     972
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     929    1182 
   Size:         87     842     253

  Fragments arranged by size: 

                 842     253      87
 
MslI CAynn'nnrTG

Cuts at:      0     136    1182 
   Size:        136    1046
 
MspA1I CmG'CkG

Cuts at:      0     865    1182 
   Size:        865     317
 
PvuII CAG'CTG

Cuts at:      0     865    1182 
   Size:        865     317
 
SmlI C'TyrA_G

Cuts at:      0     443    1182 
   Size:        443     739
 
SspI AAT'ATT

Cuts at:      0     678    1182 
   Size:        678     504
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1057    1182 
   Size:       1057     125
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     318     350     470     502    1182 
   Size:        318      32     120      32     680

  Fragments arranged by size: 

                 680     318     120      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     635    1182 
   Size:        635     547
 

 Enzymes that do cut:

     AccI     AleI     AloI    AlwNI     ApoI     BaeI     BanI     BbsI
    BciVI     BclI     BglI    BglII     BmrI     BpmI    BpuEI    BsaAI
    BsaWI    BsaXI    BseYI     BsgI    BspHI    BstXI    BstYI   DraIII
  Eco57MI    EcoRI    EcoRV    Hin4I   HincII  HindIII     HpaI     MmeI
     MslI   MspA1I    PvuII     SmlI     SspI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI    BfrBI
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaBI
    BsaHI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI