Restriction map for
pF1KB4667
(Linear) (Six-base) MAPSORT of: KIBB4667.seq Check: 3385 from: 1 to: 1065
KIBB4667.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 74 982 1065
Size: 74 908 83
Fragments arranged by size:
908 83 74
AatII G_ACGT'C
Cuts at: 0 1028 1065
Size: 1028 37
AccI GT'mk_AC
Cuts at: 0 905 1065
Size: 905 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1065
Size: 405 660
AflIII A'CryG_T
Cuts at: 0 884 1065
Size: 884 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 338 432 1065
Size: 338 94 633
Fragments arranged by size:
633 338 94
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 1065
Size: 550 32 483
Fragments arranged by size:
550 483 32
ApaI G_GGCC'C
Cuts at: 0 261 337 1065
Size: 261 76 728
Fragments arranged by size:
728 261 76
AvaI C'yCGr_G
Cuts at: 0 364 533 865 1065
Size: 364 169 332 200
Fragments arranged by size:
364 332 200 169
BamHI G'GATC_C
Cuts at: 0 635 1065
Size: 635 430
BanI G'GyrC_C
Cuts at: 0 118 1065
Size: 118 947
BanII G_rGCy'C
Cuts at: 0 261 337 584 683 1065
Size: 261 76 247 99 382
Fragments arranged by size:
382 261 247 99 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 932 999 1065
Size: 932 67 66
Fragments arranged by size:
932 67 66
BglII A'GATC_T
Cuts at: 0 956 1065
Size: 956 109
BlpI GC'TnA_GC
Cuts at: 0 682 1065
Size: 682 383
Bme1580I G_kGCm'C
Cuts at: 0 121 261 337 411 1065
Size: 121 140 76 74 654
Fragments arranged by size:
654 140 121 76 74
BmgBI CAC'GTC
Cuts at: 0 603 1065
Size: 603 462
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 978 1065
Size: 978 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 414 466 499 1065
Size: 208 206 52 33 566
Fragments arranged by size:
566 208 206 52 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1065
Size: 1030 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 940 1065
Size: 940 125
BsaAI yAC'GTr
Cuts at: 0 887 1065
Size: 887 178
BsaBI GATnn'nnATC
Cuts at: 0 160 1065
Size: 160 905
BsaHI Gr'CG_yC
Cuts at: 0 653 1025 1065
Size: 653 372 40
Fragments arranged by size:
653 372 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1065
Size: 550 30 485
Fragments arranged by size:
550 485 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 907 1065
Size: 907 158
BseYI C'CCAG_C
Cuts at: 0 114 372 1065
Size: 114 258 693
Fragments arranged by size:
693 258 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1065
Size: 334 731
BsiEI CG_ry'CG
Cuts at: 0 297 1065
Size: 297 768
BsiHKAI G_wGCw'C
Cuts at: 0 142 584 683 1065
Size: 142 442 99 382
Fragments arranged by size:
442 382 142 99
BsiWI C'GTAC_G
Cuts at: 0 887 1065
Size: 887 178
Bsp1286I G_dGCh'C
Cuts at: 0 121 142 261 337 411 584 683 1065
Size: 121 21 119 76 74 173 99 382
Fragments arranged by size:
382 173 121 119 99 76 74 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 827 982 1065
Size: 74 753 155 83
Fragments arranged by size:
753 155 83 74
BsrBI CCG'CTC
Cuts at: 0 428 849 1065
Size: 428 421 216
Fragments arranged by size:
428 421 216
BstYI r'GATC_y
Cuts at: 0 635 833 956 1065
Size: 635 198 123 109
Fragments arranged by size:
635 198 123 109
Bsu36I CC'TnA_GG
Cuts at: 0 271 1065
Size: 271 794
BtgI C'CryG_G
Cuts at: 0 545 1065
Size: 545 520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 471 1065
Size: 471 594
BtsI GCAGTG_nn'
Cuts at: 0 639 1065
Size: 639 426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 534 569 1065
Size: 534 35 496
Fragments arranged by size:
534 496 35
EaeI y'GGCC_r
Cuts at: 0 263 1065
Size: 263 802
EarI CTCTTCn'nnn_
Cuts at: 0 805 1065
Size: 805 260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 826 1065
Size: 826 239
EcoICRI GAG'CTC
Cuts at: 0 582 681 1065
Size: 582 99 384
Fragments arranged by size:
582 384 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 405 414 466 499 1065
Size: 208 197 9 52 33 566
Fragments arranged by size:
566 208 197 52 33 9
EcoO109I rG'GnC_Cy
Cuts at: 0 258 334 816 1065
Size: 258 76 482 249
Fragments arranged by size:
482 258 249 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 588 1065
Size: 588 477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1065
Size: 706 359
MspA1I CmG'CkG
Cuts at: 0 84 173 340 496 686 901 915 1065
Size: 84 89 167 156 190 215 14 150
Fragments arranged by size:
215 190 167 156 150 89 84 14
PasI CC'CwG_GG
Cuts at: 0 403 1065
Size: 403 662
PfoI T'CCnGG_A
Cuts at: 0 46 1065
Size: 46 1019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 1065
Size: 550 32 483
Fragments arranged by size:
550 483 32
PpuMI rG'GwC_Cy
Cuts at: 0 816 1065
Size: 816 249
PshAI GACnn'nnGTC
Cuts at: 0 1024 1065
Size: 1024 41
PspOMI G'GGCC_C
Cuts at: 0 257 333 1065
Size: 257 76 732
Fragments arranged by size:
732 257 76
PvuII CAG'CTG
Cuts at: 0 496 1065
Size: 496 569
SacI G_AGCT'C
Cuts at: 0 584 683 1065
Size: 584 99 382
Fragments arranged by size:
584 382 99
SmaI CCC'GGG
Cuts at: 0 535 1065
Size: 535 530
SmlI C'TyrA_G
Cuts at: 0 1045 1065
Size: 1045 20
StuI AGG'CCT
Cuts at: 0 193 415 1065
Size: 193 222 650
Fragments arranged by size:
650 222 193
StyI C'CwwG_G
Cuts at: 0 950 1065
Size: 950 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 1065
Size: 364 701
TatI w'GTAC_w
Cuts at: 0 203 869 1049 1065
Size: 203 666 180 16
Fragments arranged by size:
666 203 180 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 1065
Size: 552 513
XmaI C'CCGG_G
Cuts at: 0 533 1065
Size: 533 532
XmnI GAAnn'nnTTC
Cuts at: 0 565 1065
Size: 565 500
ZraI GAC'GTC
Cuts at: 0 1026 1065
Size: 1026 39
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AloI ApaI
AvaI BamHI BanI BanII BbsI BglII BlpI Bme1580I
BmgBI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspMI BsrBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI MmeI
MspA1I PasI PfoI PpiI PpuMI PshAI PspOMI PvuII
SacI SmaI SmlI StuI StyI TaqII TatI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BmrI BmtI
Bpu10I BsaWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
ClaI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XhoI