Restriction map for
pF1KB4674
(Linear) (Six-base) MAPSORT of: KIBB4674.seq Check: 61 from: 1 to: 1176
KIBB4674.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 138 1176
Size: 138 1038
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1176
Size: 620 556
AlwNI CAG_nnn'CTG
Cuts at: 0 55 1176
Size: 55 1121
ApoI r'AATT_y
Cuts at: 0 437 1176
Size: 437 739
AvaI C'yCGr_G
Cuts at: 0 185 1176
Size: 185 991
BanII G_rGCy'C
Cuts at: 0 185 579 1176
Size: 185 394 597
Fragments arranged by size:
597 394 185
BbvCI CC'TCA_GC
Cuts at: 0 56 1176
Size: 56 1120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 86 1176
Size: 52 34 1090
Fragments arranged by size:
1090 52 34
BclI T'GATC_A
Cuts at: 0 471 956 1166 1176
Size: 471 485 210 10
Fragments arranged by size:
485 471 210 10
BfrBI ATG'CAT
Cuts at: 0 683 1176
Size: 683 493
Bme1580I G_kGCm'C
Cuts at: 0 65 835 1176
Size: 65 770 341
Fragments arranged by size:
770 341 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1176
Size: 437 739
Bpu10I CC'TnA_GC
Cuts at: 0 40 56 1176
Size: 40 16 1120
Fragments arranged by size:
1120 40 16
BsaBI GATnn'nnATC
Cuts at: 0 695 1176
Size: 695 481
BsaHI Gr'CG_yC
Cuts at: 0 69 1176
Size: 69 1107
BsaWI w'CCGG_w
Cuts at: 0 177 545 1176
Size: 177 368 631
Fragments arranged by size:
631 368 177
Bsp1286I G_dGCh'C
Cuts at: 0 65 185 579 835 1176
Size: 65 120 394 256 341
Fragments arranged by size:
394 341 256 120 65
BspEI T'CCGG_A
Cuts at: 0 177 1176
Size: 177 999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 1176
Size: 138 1038
BsrDI GCAATG_nn'
Cuts at: 0 428 615 1176
Size: 428 187 561
Fragments arranged by size:
561 428 187
BsrFI r'CCGG_y
Cuts at: 0 22 1176
Size: 22 1154
BssSI C'ACGA_G
Cuts at: 0 985 1176
Size: 985 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 610 1176
Size: 610 566
BstBI TT'CG_AA
Cuts at: 0 1158 1176
Size: 1158 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1136 1176
Size: 1136 40
BstYI r'GATC_y
Cuts at: 0 174 261 516 1176
Size: 174 87 255 660
Fragments arranged by size:
660 255 174 87
BtgI C'CryG_G
Cuts at: 0 866 1176
Size: 866 310
EaeI y'GGCC_r
Cuts at: 0 254 1176
Size: 254 922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 620 1176
Size: 437 183 556
Fragments arranged by size:
556 437 183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 688 898 1176
Size: 688 210 278
Fragments arranged by size:
688 278 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 962 994 1176
Size: 383 32 547 32 182
Fragments arranged by size:
547 383 182 32 32
HindIII A'AGCT_T
Cuts at: 0 880 1176
Size: 880 296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 1176
Size: 369 807
MscI TGG'CCA
Cuts at: 0 256 1176
Size: 256 920
MspA1I CmG'CkG
Cuts at: 0 742 1176
Size: 742 434
NaeI GCC'GGC
Cuts at: 0 24 1176
Size: 24 1152
NcoI C'CATG_G
Cuts at: 0 866 1176
Size: 866 310
NgoMIV G'CCGG_C
Cuts at: 0 22 1176
Size: 22 1154
NsiI A_TGCA'T
Cuts at: 0 685 1176
Size: 685 491
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 707 739 1176
Size: 707 32 437
Fragments arranged by size:
707 437 32
PstI C_TGCA'G
Cuts at: 0 273 1176
Size: 273 903
SfcI C'TryA_G
Cuts at: 0 269 755 1028 1176
Size: 269 486 273 148
Fragments arranged by size:
486 273 269 148
SmaI CCC'GGG
Cuts at: 0 187 1176
Size: 187 989
SspI AAT'ATT
Cuts at: 0 278 1176
Size: 278 898
StyI C'CwwG_G
Cuts at: 0 239 553 866 925 1176
Size: 239 314 313 59 251
Fragments arranged by size:
314 313 251 239 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 617 649 1083 1115 1176
Size: 617 32 434 32 61
Fragments arranged by size:
617 434 61 32 32
XmaI C'CCGG_G
Cuts at: 0 185 1176
Size: 185 991
XmnI GAAnn'nnTTC
Cuts at: 0 217 1176
Size: 217 959
Enzymes that do cut:
AarI AcuI AlwNI ApoI AvaI BanII BbvCI BcgI
BclI BfrBI Bme1580I BpmI Bpu10I BsaBI BsaHI BsaWI
Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI EaeI Eco57MI FalI Hin4I HindIII
MmeI MscI MspA1I NaeI NcoI NgoMIV NsiI PsrI
PstI SfcI SmaI SspI StyI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BciVI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MslI NarI NdeI NheI NotI
NruI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI ZraI