Restriction map for
pF1KB4675
(Linear) (Six-base) MAPSORT of: KIBB4675.seq Check: 3247 from: 1 to: 1482
KIBB4675.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 400 1482
Size: 400 1082
AclI AA'CG_TT
Cuts at: 0 910 1482
Size: 910 572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 425 800 802 865 1280 1482
Size: 410 15 375 2 63 415 202
Fragments arranged by size:
415 410 375 202 63 15 2
AflII C'TTAA_G
Cuts at: 0 1030 1482
Size: 1030 452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 938 1482
Size: 938 544
AlwNI CAG_nnn'CTG
Cuts at: 0 778 1482
Size: 778 704
ApoI r'AATT_y
Cuts at: 0 40 1042 1117 1482
Size: 40 1002 75 365
Fragments arranged by size:
1002 365 75 40
BanI G'GyrC_C
Cuts at: 0 412 1482
Size: 412 1070
BbsI GAAGACnn'nnnn_
Cuts at: 0 252 275 1482
Size: 252 23 1207
Fragments arranged by size:
1207 252 23
BbvCI CC'TCA_GC
Cuts at: 0 246 1482
Size: 246 1236
BciVI GTATCCnnnnn_n'
Cuts at: 0 605 1240 1482
Size: 605 635 242
Fragments arranged by size:
635 605 242
BlpI GC'TnA_GC
Cuts at: 0 92 823 1482
Size: 92 731 659
Fragments arranged by size:
731 659 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 696 1482
Size: 696 786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1482
Size: 131 1351
Bpu10I CC'TnA_GC
Cuts at: 0 246 1482
Size: 246 1236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1482
Size: 438 1044
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 672 1482
Size: 672 810
BsmI GAATG_Cn'
Cuts at: 0 354 1023 1482
Size: 354 669 459
Fragments arranged by size:
669 459 354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 400 1482
Size: 400 1082
BsrBI CCG'CTC
Cuts at: 0 6 1482
Size: 6 1476
BsrFI r'CCGG_y
Cuts at: 0 953 1482
Size: 953 529
BsrGI T'GTAC_A
Cuts at: 0 769 1075 1482
Size: 769 306 407
Fragments arranged by size:
769 407 306
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 1482
Size: 534 948
BstXI CCAn_nnnn'nTGG
Cuts at: 0 981 1482
Size: 981 501
BstYI r'GATC_y
Cuts at: 0 1125 1482
Size: 1125 357
BtgI C'CryG_G
Cuts at: 0 485 1482
Size: 485 997
ClaI AT'CG_AT
Cuts at: 0 728 1482
Size: 728 754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1224 1259 1482
Size: 1224 35 223
Fragments arranged by size:
1224 223 35
EarI CTCTTCn'nnn_
Cuts at: 0 236 1460 1482
Size: 236 1224 22
Fragments arranged by size:
1224 236 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 410 425 800 802 865 1280 1482
Size: 131 279 15 375 2 63 415 202
Fragments arranged by size:
415 375 279 202 131 63 15 2
EcoRV GAT'ATC
Cuts at: 0 1386 1482
Size: 1386 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 412 1482
Size: 412 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 994 1026 1216 1248 1482
Size: 670 32 292 32 190 32 234
Fragments arranged by size:
670 292 234 190 32 32 32
HincII GTy'rAC
Cuts at: 0 107 1482
Size: 107 1375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 931 1037 1482
Size: 589 342 106 445
Fragments arranged by size:
589 445 342 106
MslI CAynn'nnrTG
Cuts at: 0 979 1482
Size: 979 503
MspA1I CmG'CkG
Cuts at: 0 96 1482
Size: 96 1386
NaeI GCC'GGC
Cuts at: 0 955 1482
Size: 955 527
NcoI C'CATG_G
Cuts at: 0 485 1482
Size: 485 997
NgoMIV G'CCGG_C
Cuts at: 0 953 1482
Size: 953 529
PshAI GACnn'nnGTC
Cuts at: 0 112 1482
Size: 112 1370
PsiI TTA'TAA
Cuts at: 0 998 1482
Size: 998 484
PstI C_TGCA'G
Cuts at: 0 462 530 1482
Size: 462 68 952
Fragments arranged by size:
952 462 68
PvuII CAG'CTG
Cuts at: 0 96 1482
Size: 96 1386
SapI GCTCTTCn'nnn_
Cuts at: 0 236 1482
Size: 236 1246
ScaI AGT'ACT
Cuts at: 0 1268 1482
Size: 1268 214
SfcI C'TryA_G
Cuts at: 0 458 526 1482
Size: 458 68 956
Fragments arranged by size:
956 458 68
SmlI C'TyrA_G
Cuts at: 0 417 1030 1482
Size: 417 613 452
Fragments arranged by size:
613 452 417
StyI C'CwwG_G
Cuts at: 0 327 485 1482
Size: 327 158 997
Fragments arranged by size:
997 327 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1305 1482
Size: 1305 177
TatI w'GTAC_w
Cuts at: 0 769 1075 1266 1482
Size: 769 306 191 216
Fragments arranged by size:
769 306 216 191
Tth111I GACn'n_nGTC
Cuts at: 0 1360 1482
Size: 1360 122
XbaI T'CTAG_A
Cuts at: 0 1128 1482
Size: 1128 354
Enzymes that do cut:
AarI AclI AcuI AflII AlfI AlwNI ApoI BanI
BbsI BbvCI BciVI BlpI BplI BpmI Bpu10I BpuEI
BseRI BsmI BspMI BsrBI BsrFI BsrGI BstAPI BstXI
BstYI BtgI ClaI CspCI EarI Eco57MI EcoRV FalI
Hin4I HincII MmeI MslI MspA1I NaeI NcoI NgoMIV
PshAI PsiI PstI PvuII SapI ScaI SfcI SmlI
StyI TaqII TatI Tth111I XbaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BcgI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BmtI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BsrDI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XcmI XhoI XmaI XmnI ZraI