Restriction map for
pF1KB4679
(Linear) (Six-base) MAPSORT of: KIBB4679.seq Check: 3282 from: 1 to: 603
KIBB4679.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 421 603
Size: 421 182
Acc65I G'GTAC_C
Cuts at: 0 242 603
Size: 242 361
AflIII A'CryG_T
Cuts at: 0 101 562 603
Size: 101 461 41
Fragments arranged by size:
461 101 41
AleI CACnn'nnGTG
Cuts at: 0 422 603
Size: 422 181
AvaI C'yCGr_G
Cuts at: 0 323 603
Size: 323 280
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 583 603
Size: 583 20
BanI G'GyrC_C
Cuts at: 0 242 603
Size: 242 361
BanII G_rGCy'C
Cuts at: 0 321 603
Size: 321 282
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 603
Size: 209 394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 456 490 603
Size: 456 34 113
Fragments arranged by size:
456 113 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 584 603
Size: 584 19
BglI GCCn_nnn'nGGC
Cuts at: 0 293 603
Size: 293 310
BglII A'GATC_T
Cuts at: 0 541 603
Size: 541 62
BmgBI CAC'GTC
Cuts at: 0 102 603
Size: 102 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 603
Size: 56 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 603
Size: 124 479
BsaAI yAC'GTr
Cuts at: 0 57 565 603
Size: 57 508 38
Fragments arranged by size:
508 57 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 603
Size: 29 574
BsiEI CG_ry'CG
Cuts at: 0 276 603
Size: 276 327
BsiHKAI G_wGCw'C
Cuts at: 0 321 603
Size: 321 282
BsmBI CGTCTCn'nnnn_
Cuts at: 0 188 603
Size: 188 415
Bsp1286I G_dGCh'C
Cuts at: 0 321 603
Size: 321 282
BspHI T'CATG_A
Cuts at: 0 546 603
Size: 546 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 421 603
Size: 421 182
BstBI TT'CG_AA
Cuts at: 0 516 603
Size: 516 87
BstYI r'GATC_y
Cuts at: 0 541 603
Size: 541 62
BtgI C'CryG_G
Cuts at: 0 150 276 603
Size: 150 126 327
Fragments arranged by size:
327 150 126
DraI TTT'AAA
Cuts at: 0 410 603
Size: 410 193
EaeI y'GGCC_r
Cuts at: 0 273 603
Size: 273 330
EagI C'GGCC_G
Cuts at: 0 273 603
Size: 273 330
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 329 603
Size: 329 274
EcoICRI GAG'CTC
Cuts at: 0 319 603
Size: 319 284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 603
Size: 56 547
EcoO109I rG'GnC_Cy
Cuts at: 0 186 603
Size: 186 417
HaeII r_GCGC'y
Cuts at: 0 122 603
Size: 122 481
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 356 388 603
Size: 356 32 215
Fragments arranged by size:
356 215 32
KpnI G_GTAC'C
Cuts at: 0 246 603
Size: 246 357
MslI CAynn'nnrTG
Cuts at: 0 55 422 603
Size: 55 367 181
Fragments arranged by size:
367 181 55
MspA1I CmG'CkG
Cuts at: 0 152 603
Size: 152 451
NruI TCG'CGA
Cuts at: 0 221 603
Size: 221 382
NspI r_CATG'y
Cuts at: 0 423 603
Size: 423 180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 603
Size: 88 32 483
Fragments arranged by size:
483 88 32
PpuMI rG'GwC_Cy
Cuts at: 0 186 603
Size: 186 417
SacI G_AGCT'C
Cuts at: 0 321 603
Size: 321 282
SacII CC_GC'GG
Cuts at: 0 153 603
Size: 153 450
SmlI C'TyrA_G
Cuts at: 0 139 603
Size: 139 464
StyI C'CwwG_G
Cuts at: 0 86 603
Size: 86 517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 603
Size: 31 572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 395 603
Size: 395 208
Enzymes that do cut:
AarI Acc65I AflIII AleI AvaI BaeI BanI BanII
BbsI BcgI BciVI BglI BglII BmgBI BpmI BpuEI
BsaAI BsaXI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BstBI BstYI BtgI DraI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KpnI MslI MspA1I NruI
NspI PpiI PpuMI SacI SacII SmlI StyI TstI
XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BbvCI BclI BfrBI BlpI
Bme1580I BmrI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiWI BsmI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI