Restriction map for
pF1KB4680
(Linear) (Six-base) MAPSORT of: KIBB4680.seq Check: 4839 from: 1 to: 1566
KIBB4680.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 801 1566
Size: 801 765
AccI GT'mk_AC
Cuts at: 0 21 1566
Size: 21 1545
AflIII A'CryG_T
Cuts at: 0 314 545 1566
Size: 314 231 1021
Fragments arranged by size:
1021 314 231
AleI CACnn'nnGTG
Cuts at: 0 685 1566
Size: 685 881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 1566
Size: 823 743
AlwNI CAG_nnn'CTG
Cuts at: 0 39 622 644 1529 1566
Size: 39 583 22 885 37
Fragments arranged by size:
885 583 39 37 22
ApaLI G'TGCA_C
Cuts at: 0 1375 1566
Size: 1375 191
ApoI r'AATT_y
Cuts at: 0 238 1566
Size: 238 1328
AvaI C'yCGr_G
Cuts at: 0 1296 1566
Size: 1296 270
BanII G_rGCy'C
Cuts at: 0 1093 1566
Size: 1093 473
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 1125 1255 1566
Size: 290 835 130 311
Fragments arranged by size:
835 311 290 130
BglI GCCn_nnn'nGGC
Cuts at: 0 940 1097 1566
Size: 940 157 469
Fragments arranged by size:
940 469 157
BglII A'GATC_T
Cuts at: 0 1103 1566
Size: 1103 463
Bme1580I G_kGCm'C
Cuts at: 0 1117 1229 1379 1566
Size: 1117 112 150 187
Fragments arranged by size:
1117 187 150 112
BmgBI CAC'GTC
Cuts at: 0 288 789 1566
Size: 288 501 777
Fragments arranged by size:
777 501 288
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 461 1317 1566
Size: 93 368 856 249
Fragments arranged by size:
856 368 249 93
BmtI G_CTAG'C
Cuts at: 0 295 1566
Size: 295 1271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 447 1566
Size: 447 1119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1566
Size: 244 1322
Bpu10I CC'TnA_GC
Cuts at: 0 1087 1165 1357 1566
Size: 1087 78 192 209
Fragments arranged by size:
1087 209 192 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1258 1535 1566
Size: 987 271 277 31
Fragments arranged by size:
987 277 271 31
BsaAI yAC'GTr
Cuts at: 0 1232 1566
Size: 1232 334
BseYI C'CCAG_C
Cuts at: 0 511 1138 1566
Size: 511 627 428
Fragments arranged by size:
627 511 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 829 1566
Size: 60 769 737
Fragments arranged by size:
769 737 60
BsiEI CG_ry'CG
Cuts at: 0 533 1566
Size: 533 1033
BsiHKAI G_wGCw'C
Cuts at: 0 907 1171 1237 1379 1566
Size: 907 264 66 142 187
Fragments arranged by size:
907 264 187 142 66
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1080 1566
Size: 1080 486
Bsp1286I G_dGCh'C
Cuts at: 0 907 1093 1117 1171 1229 1237 1379 1566
Size: 907 186 24 54 58 8 142 187
Fragments arranged by size:
907 187 186 142 58 54 24 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 801 1409 1566
Size: 801 608 157
Fragments arranged by size:
801 608 157
BsrFI r'CCGG_y
Cuts at: 0 930 1566
Size: 930 636
BsrGI T'GTAC_A
Cuts at: 0 1265 1442 1566
Size: 1265 177 124
Fragments arranged by size:
1265 177 124
BssSI C'ACGA_G
Cuts at: 0 1282 1566
Size: 1282 284
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1325 1409 1566
Size: 1325 84 157
Fragments arranged by size:
1325 157 84
BstYI r'GATC_y
Cuts at: 0 1103 1157 1566
Size: 1103 54 409
Fragments arranged by size:
1103 409 54
Bsu36I CC'TnA_GG
Cuts at: 0 949 1566
Size: 949 617
BtgI C'CryG_G
Cuts at: 0 365 1094 1370 1566
Size: 365 729 276 196
Fragments arranged by size:
729 365 276 196
DraIII CAC_nnn'GTG
Cuts at: 0 1325 1566
Size: 1325 241
DrdI GACnn_nn'nnGTC
Cuts at: 0 727 1566
Size: 727 839
EaeI y'GGCC_r
Cuts at: 0 530 1566
Size: 530 1036
EagI C'GGCC_G
Cuts at: 0 530 1566
Size: 530 1036
EarI CTCTTCn'nnn_
Cuts at: 0 783 1566
Size: 783 783
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 608 1566
Size: 608 958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1566
Size: 244 1322
EcoNI CCTnn'n_nnAGG
Cuts at: 0 687 808 952 1566
Size: 687 121 144 614
Fragments arranged by size:
687 614 144 121
EcoO109I rG'GnC_Cy
Cuts at: 0 1437 1566
Size: 1437 129
EcoRI G'AATT_C
Cuts at: 0 238 1566
Size: 238 1328
HaeII r_GCGC'y
Cuts at: 0 1281 1566
Size: 1281 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 649 681 1566
Size: 649 32 885
Fragments arranged by size:
885 649 32
HincII GTy'rAC
Cuts at: 0 708 1221 1566
Size: 708 513 345
Fragments arranged by size:
708 513 345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1566
Size: 1103 463
MslI CAynn'nnrTG
Cuts at: 0 317 550 685 1566
Size: 317 233 135 881
Fragments arranged by size:
881 317 233 135
MspA1I CmG'CkG
Cuts at: 0 168 298 622 1096 1566
Size: 168 130 324 474 470
Fragments arranged by size:
474 470 324 168 130
NaeI GCC'GGC
Cuts at: 0 932 1566
Size: 932 634
NgoMIV G'CCGG_C
Cuts at: 0 930 1566
Size: 930 636
NheI G'CTAG_C
Cuts at: 0 291 1566
Size: 291 1275
NspI r_CATG'y
Cuts at: 0 141 318 549 1266 1319 1566
Size: 141 177 231 717 53 247
Fragments arranged by size:
717 247 231 177 141 53
PciI A'CATG_T
Cuts at: 0 314 545 1566
Size: 314 231 1021
Fragments arranged by size:
1021 314 231
PflMI CCAn_nnn'nTGG
Cuts at: 0 1371 1566
Size: 1371 195
PmlI CAC'GTG
Cuts at: 0 1232 1566
Size: 1232 334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 170 202 1405 1437 1566
Size: 170 32 1203 32 129
Fragments arranged by size:
1203 170 129 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1437 1566
Size: 1437 129
PstI C_TGCA'G
Cuts at: 0 649 1157 1566
Size: 649 508 409
Fragments arranged by size:
649 508 409
PvuII CAG'CTG
Cuts at: 0 168 298 622 1566
Size: 168 130 324 944
Fragments arranged by size:
944 324 168 130
SacII CC_GC'GG
Cuts at: 0 1097 1566
Size: 1097 469
ScaI AGT'ACT
Cuts at: 0 1345 1566
Size: 1345 221
SexAI A'CCwGG_T
Cuts at: 0 803 1566
Size: 803 763
SfcI C'TryA_G
Cuts at: 0 31 645 1153 1566
Size: 31 614 508 413
Fragments arranged by size:
614 508 413 31
SmlI C'TyrA_G
Cuts at: 0 966 1273 1550 1566
Size: 966 307 277 16
Fragments arranged by size:
966 307 277 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 951 1336 1479 1566
Size: 951 385 143 87
Fragments arranged by size:
951 385 143 87
TatI w'GTAC_w
Cuts at: 0 120 1265 1343 1442 1566
Size: 120 1145 78 99 124
Fragments arranged by size:
1145 124 120 99 78
Tth111I GACn'n_nGTC
Cuts at: 0 325 418 961 1566
Size: 325 93 543 605
Fragments arranged by size:
605 543 325 93
XmnI GAAnn'nnTTC
Cuts at: 0 1252 1566
Size: 1252 314
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApaLI ApoI
AvaI BanII BbsI BglI BglII Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI BssSI
BstAPI BstYI Bsu36I BtgI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII MmeI MslI MspA1I NaeI NgoMIV NheI NspI
PciI PflMI PmlI PpiI PpuMI PstI PvuII SacII
ScaI SexAI SfcI SmlI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BssHII BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI EcoICRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NotI NruI
NsiI PacI PasI PfoI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI XbaI
XcmI XhoI XmaI ZraI