Restriction map for
pF1KB4688
(Linear) (Six-base) MAPSORT of: KIBB4688.seq Check: 9854 from: 1 to: 1731
KIBB4688.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 954 1731
Size: 954 777
AccI GT'mk_AC
Cuts at: 0 144 1731
Size: 144 1587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1415 1731
Size: 766 649 316
Fragments arranged by size:
766 649 316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1256 1731
Size: 1256 475
AlwNI CAG_nnn'CTG
Cuts at: 0 1236 1731
Size: 1236 495
ApoI r'AATT_y
Cuts at: 0 600 1247 1546 1731
Size: 600 647 299 185
Fragments arranged by size:
647 600 299 185
AvaI C'yCGr_G
Cuts at: 0 339 614 1264 1367 1731
Size: 339 275 650 103 364
Fragments arranged by size:
650 364 339 275 103
BamHI G'GATC_C
Cuts at: 0 473 1731
Size: 473 1258
BanI G'GyrC_C
Cuts at: 0 1000 1260 1387 1731
Size: 1000 260 127 344
Fragments arranged by size:
1000 344 260 127
BanII G_rGCy'C
Cuts at: 0 491 1731
Size: 491 1240
BbsI GAAGACnn'nnnn_
Cuts at: 0 1117 1731
Size: 1117 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 439 473 1731
Size: 439 34 1258
Fragments arranged by size:
1258 439 34
BclI T'GATC_A
Cuts at: 0 1586 1731
Size: 1586 145
BglI GCCn_nnn'nGGC
Cuts at: 0 501 1731
Size: 501 1230
Bme1580I G_kGCm'C
Cuts at: 0 1003 1265 1390 1731
Size: 1003 262 125 341
Fragments arranged by size:
1003 341 262 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1066 1731
Size: 526 540 665
Fragments arranged by size:
665 540 526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1081 1731
Size: 757 324 650
Fragments arranged by size:
757 650 324
BsaI GGTCTCn'nnnn_
Cuts at: 0 1399 1455 1731
Size: 1399 56 276
Fragments arranged by size:
1399 276 56
BsaAI yAC'GTr
Cuts at: 0 1440 1731
Size: 1440 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1731
Size: 895 30 806
Fragments arranged by size:
895 806 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 338 754 910 1120 1560 1731
Size: 338 416 156 210 440 171
Fragments arranged by size:
440 416 338 210 171 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1731
Size: 215 1516
Bsp1286I G_dGCh'C
Cuts at: 0 491 1003 1265 1390 1731
Size: 491 512 262 125 341
Fragments arranged by size:
512 491 341 262 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 130 954 1731
Size: 130 824 777
Fragments arranged by size:
824 777 130
BsrDI GCAATG_nn'
Cuts at: 0 1032 1731
Size: 1032 699
BssSI C'ACGA_G
Cuts at: 0 1458 1731
Size: 1458 273
BstYI r'GATC_y
Cuts at: 0 473 878 1049 1731
Size: 473 405 171 682
Fragments arranged by size:
682 473 405 171
BtgI C'CryG_G
Cuts at: 0 1256 1346 1446 1593 1731
Size: 1256 90 100 147 138
Fragments arranged by size:
1256 147 138 100 90
BtsI GCAGTG_nn'
Cuts at: 0 983 1731
Size: 983 748
DraI TTT'AAA
Cuts at: 0 1415 1731
Size: 1415 316
EaeI y'GGCC_r
Cuts at: 0 843 1016 1731
Size: 843 173 715
Fragments arranged by size:
843 715 173
EarI CTCTTCn'nnn_
Cuts at: 0 83 513 1434 1731
Size: 83 430 921 297
Fragments arranged by size:
921 430 297 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 766 1066 1415 1731
Size: 526 240 300 349 316
Fragments arranged by size:
526 349 316 300 240
EcoRI G'AATT_C
Cuts at: 0 1247 1731
Size: 1247 484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 406 1731
Size: 406 1325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 1203 1235 1600 1632 1731
Size: 271 32 900 32 365 32 99
Fragments arranged by size:
900 365 271 99 32 32 32
HincII GTy'rAC
Cuts at: 0 279 1731
Size: 279 1452
MfeI C'AATT_G
Cuts at: 0 1563 1731
Size: 1563 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 1646 1731
Size: 954 692 85
Fragments arranged by size:
954 692 85
MscI TGG'CCA
Cuts at: 0 845 1018 1731
Size: 845 173 713
Fragments arranged by size:
845 713 173
MspA1I CmG'CkG
Cuts at: 0 199 1236 1731
Size: 199 1037 495
Fragments arranged by size:
1037 495 199
NcoI C'CATG_G
Cuts at: 0 1256 1446 1593 1731
Size: 1256 190 147 138
Fragments arranged by size:
1256 190 147 138
NspI r_CATG'y
Cuts at: 0 1044 1731
Size: 1044 687
PflMI CCAn_nnn'nTGG
Cuts at: 0 841 1135 1629 1731
Size: 841 294 494 102
Fragments arranged by size:
841 494 294 102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1731
Size: 1189 32 510
Fragments arranged by size:
1189 510 32
PshAI GACnn'nnGTC
Cuts at: 0 1710 1731
Size: 1710 21
PstI C_TGCA'G
Cuts at: 0 204 482 771 990 1731
Size: 204 278 289 219 741
Fragments arranged by size:
741 289 278 219 204
PvuII CAG'CTG
Cuts at: 0 199 1236 1731
Size: 199 1037 495
Fragments arranged by size:
1037 495 199
SfcI C'TryA_G
Cuts at: 0 200 221 478 767 986 1280 1731
Size: 200 21 257 289 219 294 451
Fragments arranged by size:
451 294 289 257 219 200 21
SmaI CCC'GGG
Cuts at: 0 616 1731
Size: 616 1115
SmlI C'TyrA_G
Cuts at: 0 5 736 1096 1731
Size: 5 731 360 635
Fragments arranged by size:
731 635 360 5
SpeI A'CTAG_T
Cuts at: 0 1186 1731
Size: 1186 545
StuI AGG'CCT
Cuts at: 0 935 1731
Size: 935 796
StyI C'CwwG_G
Cuts at: 0 653 1053 1256 1340 1446 1593 1731
Size: 653 400 203 84 106 147 138
Fragments arranged by size:
653 400 203 147 138 106 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1131 1731
Size: 1131 600
TatI w'GTAC_w
Cuts at: 0 911 1678 1731
Size: 911 767 53
Fragments arranged by size:
911 767 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1011 1444 1731
Size: 1011 433 287
Fragments arranged by size:
1011 433 287
XmaI C'CCGG_G
Cuts at: 0 614 1731
Size: 614 1117
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BcgI BclI BglI Bme1580I BpmI
BpuEI BsaI BsaAI BsaXI BseRI BsgI Bsp1286I BspMI
BsrDI BssSI BstYI BtgI BtsI DraI EaeI EarI
Eco57MI EcoRI FalI Hin4I HincII MfeI MmeI MscI
MspA1I NcoI NspI PflMI PpiI PshAI PstI PvuII
SfcI SmaI SmlI SpeI StuI StyI TaqII TatI
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BciVI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
XmnI ZraI