Restriction map for
pF1KB4689
(Linear) (Six-base) MAPSORT of: KIBB4689.seq Check: 1698 from: 1 to: 1320
KIBB4689.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 322 1320
Size: 130 192 998
Fragments arranged by size:
998 192 130
ApaLI G'TGCA_C
Cuts at: 0 590 1320
Size: 590 730
ApoI r'AATT_y
Cuts at: 0 52 296 1320
Size: 52 244 1024
Fragments arranged by size:
1024 244 52
AvaI C'yCGr_G
Cuts at: 0 250 1320
Size: 250 1070
BamHI G'GATC_C
Cuts at: 0 1213 1320
Size: 1213 107
BanII G_rGCy'C
Cuts at: 0 781 840 988 1320
Size: 781 59 148 332
Fragments arranged by size:
781 332 148 59
BglII A'GATC_T
Cuts at: 0 103 1320
Size: 103 1217
Bme1580I G_kGCm'C
Cuts at: 0 594 1320
Size: 594 726
BmgBI CAC'GTC
Cuts at: 0 589 1320
Size: 589 731
BmrI ACTGGGnnnn_n'
Cuts at: 0 670 1320
Size: 670 650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1320
Size: 935 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 875 903 911 1320
Size: 875 28 8 409
Fragments arranged by size:
875 409 28 8
BsaHI Gr'CG_yC
Cuts at: 0 672 738 1320
Size: 672 66 582
Fragments arranged by size:
672 582 66
BsaWI w'CCGG_w
Cuts at: 0 1027 1320
Size: 1027 293
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 794 1320
Size: 794 526
BseYI C'CCAG_C
Cuts at: 0 160 1320
Size: 160 1160
BsiEI CG_ry'CG
Cuts at: 0 822 1320
Size: 822 498
BsiHKAI G_wGCw'C
Cuts at: 0 594 840 1320
Size: 594 246 480
Fragments arranged by size:
594 480 246
BsmI GAATG_Cn'
Cuts at: 0 137 162 481 722 1269 1320
Size: 137 25 319 241 547 51
Fragments arranged by size:
547 319 241 137 51 25
Bsp1286I G_dGCh'C
Cuts at: 0 594 781 840 988 1320
Size: 594 187 59 148 332
Fragments arranged by size:
594 332 187 148 59
BspEI T'CCGG_A
Cuts at: 0 1027 1320
Size: 1027 293
BspHI T'CATG_A
Cuts at: 0 1170 1320
Size: 1170 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 995 1119 1320
Size: 995 124 201
Fragments arranged by size:
995 201 124
BstYI r'GATC_y
Cuts at: 0 103 1126 1213 1320
Size: 103 1023 87 107
Fragments arranged by size:
1023 107 103 87
BtsI GCAGTG_nn'
Cuts at: 0 173 249 1320
Size: 173 76 1071
Fragments arranged by size:
1071 173 76
DraI TTT'AAA
Cuts at: 0 99 1320
Size: 99 1221
DraIII CAC_nnn'GTG
Cuts at: 0 1021 1320
Size: 1021 299
EaeI y'GGCC_r
Cuts at: 0 819 1320
Size: 819 501
EagI C'GGCC_G
Cuts at: 0 819 1320
Size: 819 501
EarI CTCTTCn'nnn_
Cuts at: 0 77 320 693 753 857 897 1320
Size: 77 243 373 60 104 40 423
Fragments arranged by size:
423 373 243 104 77 60 40
EcoICRI GAG'CTC
Cuts at: 0 838 1320
Size: 838 482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 322 935 1320
Size: 130 192 613 385
Fragments arranged by size:
613 385 192 130
EcoNI CCTnn'n_nnAGG
Cuts at: 0 785 1208 1320
Size: 785 423 112
Fragments arranged by size:
785 423 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 768 800 1172 1204 1320
Size: 550 32 186 32 372 32 116
Fragments arranged by size:
550 372 186 116 32 32 32
HindIII A'AGCT_T
Cuts at: 0 463 1320
Size: 463 857
MfeI C'AATT_G
Cuts at: 0 287 1320
Size: 287 1033
MslI CAynn'nnrTG
Cuts at: 0 993 1320
Size: 993 327
MspA1I CmG'CkG
Cuts at: 0 1152 1320
Size: 1152 168
NdeI CA'TA_TG
Cuts at: 0 1065 1320
Size: 1065 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 1021 1320
Size: 1021 299
PfoI T'CCnGG_A
Cuts at: 0 470 807 1320
Size: 470 337 513
Fragments arranged by size:
513 470 337
PstI C_TGCA'G
Cuts at: 0 72 1283 1320
Size: 72 1211 37
Fragments arranged by size:
1211 72 37
SacI G_AGCT'C
Cuts at: 0 840 1320
Size: 840 480
SfcI C'TryA_G
Cuts at: 0 68 1279 1320
Size: 68 1211 41
Fragments arranged by size:
1211 68 41
SspI AAT'ATT
Cuts at: 0 192 1320
Size: 192 1128
StyI C'CwwG_G
Cuts at: 0 320 1320
Size: 320 1000
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 1320
Size: 662 32 626
Fragments arranged by size:
662 626 32
XbaI T'CTAG_A
Cuts at: 0 106 754 1320
Size: 106 648 566
Fragments arranged by size:
648 566 106
Enzymes that do cut:
AcuI ApaLI ApoI AvaI BamHI BanII BglII Bme1580I
BmgBI BmrI BpmI BsaI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BstXI BstYI
BtsI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI Hin4I HindIII MfeI MslI MspA1I NdeI PflMI
PfoI PstI SacI SfcI SspI StyI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaXI BsgI BsiWI BsmBI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DrdI EciI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MluI MmeI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TatI Tth111I XcmI
XhoI XmaI XmnI ZraI