Restriction map for
pF1KB4690
(Linear) (Six-base) MAPSORT of: KIBB4690.seq Check: 6133 from: 1 to: 2685
KIBB4690.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
Acc65I G'GTAC_C
Cuts at: 0 1991 2685
Size: 1991 694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 460 535 856 1139 1822 2510 2562
Size: 79 381 75 321 283 683 688 52
Cuts at: 2562 2685
Size: 123
Fragments arranged by size:
688 683 381 321 283 123 79 75
52
AflIII A'CryG_T
Cuts at: 0 2054 2420 2685
Size: 2054 366 265
Fragments arranged by size:
2054 366 265
AhdI GACnn_n'nnGTC
Cuts at: 0 945 2685
Size: 945 1740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 602 1546 2685
Size: 602 944 1139
Fragments arranged by size:
1139 944 602
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 710 742 2685
Size: 710 32 1943
Fragments arranged by size:
1943 710 32
AlwNI CAG_nnn'CTG
Cuts at: 0 931 1093 1710 2263 2380 2685
Size: 931 162 617 553 117 305
Fragments arranged by size:
931 617 553 305 162 117
ApaI G_GGCC'C
Cuts at: 0 386 685 694 799 2449 2685
Size: 386 299 9 105 1650 236
Fragments arranged by size:
1650 386 299 236 105 9
ApoI r'AATT_y
Cuts at: 0 266 375 861 1130 2077 2685
Size: 266 109 486 269 947 608
Fragments arranged by size:
947 608 486 269 266 109
AvaI C'yCGr_G
Cuts at: 0 16 662 2685
Size: 16 646 2023
Fragments arranged by size:
2023 646 16
BamHI G'GATC_C
Cuts at: 0 2505 2685
Size: 2505 180
BanI G'GyrC_C
Cuts at: 0 73 598 1991 2484 2685
Size: 73 525 1393 493 201
Fragments arranged by size:
1393 525 493 201 73
BanII G_rGCy'C
Cuts at: 0 123 386 530 685 694 723 799 1039
Size: 123 263 144 155 9 29 76 240
Cuts at: 1039 1142 2449 2685
Size: 103 1307 236
Fragments arranged by size:
1307 263 240 236 155 144 123 103
76 29 9
BbeI G_GCGC'C
Cuts at: 0 77 2488 2685
Size: 77 2411 197
Fragments arranged by size:
2411 197 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 1645 2192 2685
Size: 1645 547 493
Fragments arranged by size:
1645 547 493
BbvCI CC'TCA_GC
Cuts at: 0 2296 2342 2685
Size: 2296 46 343
Fragments arranged by size:
2296 343 46
BciVI GTATCCnnnnn_n'
Cuts at: 0 1943 2685
Size: 1943 742
BglI GCCn_nnn'nGGC
Cuts at: 0 71 106 182 689 2444 2654 2685
Size: 71 35 76 507 1755 210 31
Fragments arranged by size:
1755 507 210 76 71 35 31
BglII A'GATC_T
Cuts at: 0 1821 2685
Size: 1821 864
BlpI GC'TnA_GC
Cuts at: 0 449 2594 2633 2685
Size: 449 2145 39 52
Fragments arranged by size:
2145 449 52 39
Bme1580I G_kGCm'C
Cuts at: 0 386 601 685 694 799 2449 2685
Size: 386 215 84 9 105 1650 236
Fragments arranged by size:
1650 386 236 215 105 84 9
BmgBI CAC'GTC
Cuts at: 0 2055 2685
Size: 2055 630
BmrI ACTGGGnnnn_n'
Cuts at: 0 598 679 2042 2685
Size: 598 81 1363 643
Fragments arranged by size:
1363 643 598 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1074 2685
Size: 1074 1611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 583 659 779 1258 2003 2264 2685
Size: 44 539 76 120 479 745 261 421
Fragments arranged by size:
745 539 479 421 261 120 76 44
Bpu10I CC'TnA_GC
Cuts at: 0 1454 1837 2296 2342 2456 2685
Size: 1454 383 459 46 114 229
Fragments arranged by size:
1454 459 383 229 114 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 1456 2543 2685
Size: 1378 78 1087 142
Fragments arranged by size:
1378 1087 142 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 2269 2685
Size: 2269 416
BsaHI Gr'CG_yC
Cuts at: 0 74 2485 2685
Size: 74 2411 200
Fragments arranged by size:
2411 200 74
BsaWI w'CCGG_w
Cuts at: 0 971 2685
Size: 971 1714
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 710 740 2665 2685
Size: 710 30 1925 20
Fragments arranged by size:
1925 710 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 302 383 442 445 496 641 949
Size: 157 145 81 59 3 51 145 308
Cuts at: 949 1291 2329 2548 2685
Size: 342 1038 219 137
Fragments arranged by size:
1038 342 308 219 157 145 145 137
81 59 51 3
BseYI C'CCAG_C
Cuts at: 0 1007 1086 2006 2685
Size: 1007 79 920 679
Fragments arranged by size:
1007 920 679 79
BsiEI CG_ry'CG
Cuts at: 0 202 2685
Size: 202 2483
BsiHKAI G_wGCw'C
Cuts at: 0 123 530 1039 1283 2600 2685
Size: 123 407 509 244 1317 85
Fragments arranged by size:
1317 509 407 244 123 85
Bsp1286I G_dGCh'C
Cuts at: 0 123 386 530 601 685 694 723 799
Size: 123 263 144 71 84 9 29 76
Cuts at: 799 1039 1142 1283 2449 2600 2685
Size: 240 103 141 1166 151 85
Fragments arranged by size:
1166 263 240 151 144 141 123 103
85 84 76 71 29 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1538 2685
Size: 1538 1147
BsrBI CCG'CTC
Cuts at: 0 156 235 435 2043 2685
Size: 156 79 200 1608 642
Fragments arranged by size:
1608 642 200 156 79
BsrDI GCAATG_nn'
Cuts at: 0 463 2685
Size: 463 2222
BsrFI r'CCGG_y
Cuts at: 0 201 2685
Size: 201 2484
BssSI C'ACGA_G
Cuts at: 0 737 2685
Size: 737 1948
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1508 2002 2685
Size: 1508 494 683
Fragments arranged by size:
1508 683 494
BstYI r'GATC_y
Cuts at: 0 803 824 1163 1821 2505 2685
Size: 803 21 339 658 684 180
Fragments arranged by size:
803 684 658 339 180 21
BtgI C'CryG_G
Cuts at: 0 713 1071 1758 1891 2426 2649 2685
Size: 713 358 687 133 535 223 36
Fragments arranged by size:
713 687 535 358 223 133 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2335 2685
Size: 2335 350
BtsI GCAGTG_nn'
Cuts at: 0 1311 2685
Size: 1311 1374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 269 304 359 394 2685
Size: 269 35 55 35 2291
Fragments arranged by size:
2291 269 55 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1437 2685
Size: 1437 1248
DrdI GACnn_nn'nnGTC
Cuts at: 0 1647 2685
Size: 1647 1038
EaeI y'GGCC_r
Cuts at: 0 199 2513 2685
Size: 199 2314 172
Fragments arranged by size:
2314 199 172
EagI C'GGCC_G
Cuts at: 0 199 2685
Size: 199 2486
EarI CTCTTCn'nnn_
Cuts at: 0 848 872 1268 2096 2156 2162 2353 2362
Size: 848 24 396 828 60 6 191 9
Cuts at: 2362 2486 2685
Size: 124 199
Fragments arranged by size:
848 828 396 199 191 124 60 24
9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 305 2685
Size: 305 2380
EcoICRI GAG'CTC
Cuts at: 0 121 528 1037 2685
Size: 121 407 509 1648
Fragments arranged by size:
1648 509 407 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 79 460 535 583 659 779 856
Size: 44 35 381 75 48 76 120 77
Cuts at: 856 1139 1258 1822 2003 2264 2510 2562 2685
Size: 283 119 564 181 261 246 52 123
Fragments arranged by size:
564 381 283 261 246 181 123 120
119 77 76 75 52 48 44 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 335 2685
Size: 335 2350
EcoO109I rG'GnC_Cy
Cuts at: 0 382 383 658 691 796 1449 1614 1764
Size: 382 1 275 33 105 653 165 150
Cuts at: 1764 1881 2445 2685
Size: 117 564 240
Fragments arranged by size:
653 564 382 275 240 165 150 117
105 33 1
EcoRI G'AATT_C
Cuts at: 0 266 861 2685
Size: 266 595 1824
Fragments arranged by size:
1824 595 266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1182 1578 2592 2685
Size: 1182 396 1014 93
Fragments arranged by size:
1182 1014 396 93
FspI TGC'GCA
Cuts at: 0 646 2685
Size: 646 2039
FspAI rTGC'GCAy
Cuts at: 0 646 2685
Size: 646 2039
HaeII r_GCGC'y
Cuts at: 0 77 2482 2488 2685
Size: 77 2405 6 197
Fragments arranged by size:
2405 197 77 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 105 110 137 142 772 804 849
Size: 29 76 5 27 5 630 32 45
Cuts at: 849 881 886 918 931 963 2134 2166 2685
Size: 32 5 32 13 32 1171 32 519
Fragments arranged by size:
1171 630 519 76 45 32 32 32
32 32 29 27 13 5 5 5
KasI G'GCGC_C
Cuts at: 0 73 2484 2685
Size: 73 2411 201
Fragments arranged by size:
2411 201 73
KpnI G_GTAC'C
Cuts at: 0 1995 2685
Size: 1995 690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2364 2477 2685
Size: 2364 113 208
Fragments arranged by size:
2364 208 113
MscI TGG'CCA
Cuts at: 0 2515 2685
Size: 2515 170
MslI CAynn'nnrTG
Cuts at: 0 2000 2414 2431 2685
Size: 2000 414 17 254
Fragments arranged by size:
2000 414 254 17
MspA1I CmG'CkG
Cuts at: 0 42 553 834 1011 1093 1401 2685
Size: 42 511 281 177 82 308 1284
Fragments arranged by size:
1284 511 308 281 177 82 42
NarI GG'CG_CC
Cuts at: 0 74 2485 2685
Size: 74 2411 200
Fragments arranged by size:
2411 200 74
NcoI C'CATG_G
Cuts at: 0 713 1758 1891 2685
Size: 713 1045 133 794
Fragments arranged by size:
1045 794 713 133
NruI TCG'CGA
Cuts at: 0 140 2685
Size: 140 2545
NspI r_CATG'y
Cuts at: 0 2424 2542 2685
Size: 2424 118 143
Fragments arranged by size:
2424 143 118
PasI CC'CwG_GG
Cuts at: 0 909 1951 2685
Size: 909 1042 734
Fragments arranged by size:
1042 909 734
PciI A'CATG_T
Cuts at: 0 2420 2685
Size: 2420 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 343 816 2685
Size: 343 473 1869
Fragments arranged by size:
1869 473 343
PfoI T'CCnGG_A
Cuts at: 0 1598 2241 2685
Size: 1598 643 444
Fragments arranged by size:
1598 643 444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 512 544 710 742 1652 1684 2685
Size: 512 32 166 32 910 32 1001
Fragments arranged by size:
1001 910 512 166 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 658 1449 1764 1881 2685
Size: 658 791 315 117 804
Fragments arranged by size:
804 791 658 315 117
PspOMI G'GGCC_C
Cuts at: 0 382 681 690 795 2445 2685
Size: 382 299 9 105 1650 240
Fragments arranged by size:
1650 382 299 240 105 9
PspXI vC'TCGA_Gb
Cuts at: 0 662 2685
Size: 662 2023
PstI C_TGCA'G
Cuts at: 0 164 308 833 1251 1535 1622 2223 2685
Size: 164 144 525 418 284 87 601 462
Fragments arranged by size:
601 525 462 418 284 164 144 87
PvuII CAG'CTG
Cuts at: 0 42 553 834 1093 1401 2685
Size: 42 511 281 259 308 1284
Fragments arranged by size:
1284 511 308 281 259 42
SacI G_AGCT'C
Cuts at: 0 123 530 1039 2685
Size: 123 407 509 1646
Fragments arranged by size:
1646 509 407 123
SapI GCTCTTCn'nnn_
Cuts at: 0 2353 2685
Size: 2353 332
SbfI CC_TGCA'GG
Cuts at: 0 1535 1622 2685
Size: 1535 87 1063
Fragments arranged by size:
1535 1063 87
SfcI C'TryA_G
Cuts at: 0 160 304 701 829 1247 1531 1618 2219
Size: 160 144 397 128 418 284 87 601
Cuts at: 2219 2685
Size: 466
Fragments arranged by size:
601 466 418 397 284 160 144 128
87
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 689 2685
Size: 689 1996
SfoI GGC'GCC
Cuts at: 0 75 2486 2685
Size: 75 2411 199
Fragments arranged by size:
2411 199 75
SmlI C'TyrA_G
Cuts at: 0 662 1393 1471 2558 2685
Size: 662 731 78 1087 127
Fragments arranged by size:
1087 731 662 127 78
StuI AGG'CCT
Cuts at: 0 341 2685
Size: 341 2344
StyI C'CwwG_G
Cuts at: 0 342 348 713 1758 1847 1891 2119 2685
Size: 342 6 365 1045 89 44 228 566
Fragments arranged by size:
1045 566 365 342 228 89 44 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 383 394 2685
Size: 383 11 2291
Fragments arranged by size:
2291 383 11
TatI w'GTAC_w
Cuts at: 0 1769 2685
Size: 1769 916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2647 2685
Size: 2647 38
XhoI C'TCGA_G
Cuts at: 0 662 2685
Size: 662 2023
XmnI GAAnn'nnTTC
Cuts at: 0 1375 1786 2685
Size: 1375 411 899
Fragments arranged by size:
1375 899 411
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NruI
NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SapI SbfI SfcI SfiI
SfoI SmlI StuI StyI TaqII TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI
BfrBI BmtI BsaAI BsaBI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI DraI EcoRV FseI HincII HindIII HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NsiI
PacI PmeI PmlI PshAI PsiI PsrI PvuI RsrII
SacII SalI SanDI ScaI SexAI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XmaI ZraI