Restriction map for
pF1KB4697
(Linear) (Six-base) MAPSORT of: KIBB4697.seq Check: 1117 from: 1 to: 981
KIBB4697.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 894 981
Size: 894 87
AccI GT'mk_AC
Cuts at: 0 473 981
Size: 473 508
Acc65I G'GTAC_C
Cuts at: 0 710 981
Size: 710 271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 952 981
Size: 808 144 29
Fragments arranged by size:
808 144 29
AflII C'TTAA_G
Cuts at: 0 664 981
Size: 664 317
AflIII A'CryG_T
Cuts at: 0 770 981
Size: 770 211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 981
Size: 364 617
AlwNI CAG_nnn'CTG
Cuts at: 0 30 930 981
Size: 30 900 51
Fragments arranged by size:
900 51 30
ApaI G_GGCC'C
Cuts at: 0 155 981
Size: 155 826
ApoI r'AATT_y
Cuts at: 0 658 981
Size: 658 323
AvaI C'yCGr_G
Cuts at: 0 498 726 981
Size: 498 228 255
Fragments arranged by size:
498 255 228
BanI G'GyrC_C
Cuts at: 0 85 127 710 981
Size: 85 42 583 271
Fragments arranged by size:
583 271 85 42
BanII G_rGCy'C
Cuts at: 0 155 567 848 981
Size: 155 412 281 133
Fragments arranged by size:
412 281 155 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 524 665 981
Size: 318 206 141 316
Fragments arranged by size:
318 316 206 141
BbvCI CC'TCA_GC
Cuts at: 0 337 981
Size: 337 644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 414 448 981
Size: 414 34 533
Fragments arranged by size:
533 414 34
Bme1580I G_kGCm'C
Cuts at: 0 155 236 981
Size: 155 81 745
Fragments arranged by size:
745 155 81
BmgBI CAC'GTC
Cuts at: 0 771 948 981
Size: 771 177 33
Fragments arranged by size:
771 177 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 303 747 981
Size: 90 213 444 234
Fragments arranged by size:
444 234 213 90
Bpu10I CC'TnA_GC
Cuts at: 0 337 981
Size: 337 644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 835 981
Size: 723 112 146
Fragments arranged by size:
723 146 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 981
Size: 580 401
BsaHI Gr'CG_yC
Cuts at: 0 596 670 981
Size: 596 74 311
Fragments arranged by size:
596 311 74
BsaWI w'CCGG_w
Cuts at: 0 242 981
Size: 242 739
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 981
Size: 289 692
BseYI C'CCAG_C
Cuts at: 0 357 981
Size: 357 624
BsiEI CG_ry'CG
Cuts at: 0 15 44 981
Size: 15 29 937
Fragments arranged by size:
937 29 15
BsiHKAI G_wGCw'C
Cuts at: 0 567 981
Size: 567 414
Bsp1286I G_dGCh'C
Cuts at: 0 155 236 567 848 981
Size: 155 81 331 281 133
Fragments arranged by size:
331 281 155 133 81
BspEI T'CCGG_A
Cuts at: 0 242 981
Size: 242 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 894 981
Size: 894 87
BsrFI r'CCGG_y
Cuts at: 0 421 713 981
Size: 421 292 268
Fragments arranged by size:
421 292 268
BsrGI T'GTAC_A
Cuts at: 0 827 981
Size: 827 154
BssHII G'CGCG_C
Cuts at: 0 110 981
Size: 110 871
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 981
Size: 30 951
BstEII G'GTnAC_C
Cuts at: 0 493 855 981
Size: 493 362 126
Fragments arranged by size:
493 362 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 810 981
Size: 810 171
BstYI r'GATC_y
Cuts at: 0 221 386 981
Size: 221 165 595
Fragments arranged by size:
595 221 165
BtgI C'CryG_G
Cuts at: 0 15 38 140 171 590 779 809 981
Size: 15 23 102 31 419 189 30 172
Fragments arranged by size:
419 189 172 102 31 30 23 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 21 60 981
Size: 21 39 921
Fragments arranged by size:
921 39 21
BtsI GCAGTG_nn'
Cuts at: 0 234 981
Size: 234 747
EaeI y'GGCC_r
Cuts at: 0 41 71 981
Size: 41 30 910
Fragments arranged by size:
910 41 30
EagI C'GGCC_G
Cuts at: 0 41 981
Size: 41 940
EarI CTCTTCn'nnn_
Cuts at: 0 436 684 981
Size: 436 248 297
Fragments arranged by size:
436 297 248
EcoICRI GAG'CTC
Cuts at: 0 565 981
Size: 565 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 303 747 808 952 981
Size: 90 213 444 61 144 29
Fragments arranged by size:
444 213 144 90 61 29
EcoO109I rG'GnC_Cy
Cuts at: 0 152 927 981
Size: 152 775 54
Fragments arranged by size:
775 152 54
EcoRI G'AATT_C
Cuts at: 0 658 981
Size: 658 323
HaeII r_GCGC'y
Cuts at: 0 726 981
Size: 726 255
KpnI G_GTAC'C
Cuts at: 0 714 981
Size: 714 267
MscI TGG'CCA
Cuts at: 0 73 981
Size: 73 908
MslI CAynn'nnrTG
Cuts at: 0 808 981
Size: 808 173
MspA1I CmG'CkG
Cuts at: 0 17 173 981
Size: 17 156 808
Fragments arranged by size:
808 156 17
NaeI GCC'GGC
Cuts at: 0 423 981
Size: 423 558
NcoI C'CATG_G
Cuts at: 0 140 779 809 981
Size: 140 639 30 172
Fragments arranged by size:
639 172 140 30
NgoMIV G'CCGG_C
Cuts at: 0 421 981
Size: 421 560
NspI r_CATG'y
Cuts at: 0 957 981
Size: 957 24
PpuMI rG'GwC_Cy
Cuts at: 0 927 981
Size: 927 54
PspOMI G'GGCC_C
Cuts at: 0 151 981
Size: 151 830
PspXI vC'TCGA_Gb
Cuts at: 0 726 981
Size: 726 255
PstI C_TGCA'G
Cuts at: 0 26 444 905 981
Size: 26 418 461 76
Fragments arranged by size:
461 418 76 26
SacI G_AGCT'C
Cuts at: 0 567 981
Size: 567 414
SacII CC_GC'GG
Cuts at: 0 18 174 981
Size: 18 156 807
Fragments arranged by size:
807 156 18
SbfI CC_TGCA'GG
Cuts at: 0 905 981
Size: 905 76
SfcI C'TryA_G
Cuts at: 0 22 390 440 705 873 901 981
Size: 22 368 50 265 168 28 80
Fragments arranged by size:
368 265 168 80 50 28 22
SmlI C'TyrA_G
Cuts at: 0 664 726 738 850 981
Size: 664 62 12 112 131
Fragments arranged by size:
664 131 112 62 12
SphI G_CATG'C
Cuts at: 0 957 981
Size: 957 24
StuI AGG'CCT
Cuts at: 0 446 754 981
Size: 446 308 227
Fragments arranged by size:
446 308 227
StyI C'CwwG_G
Cuts at: 0 140 779 809 981
Size: 140 639 30 172
Fragments arranged by size:
639 172 140 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 349 981
Size: 199 150 632
Fragments arranged by size:
632 199 150
TatI w'GTAC_w
Cuts at: 0 101 827 981
Size: 101 726 154
Fragments arranged by size:
726 154 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 981
Size: 138 843
XhoI C'TCGA_G
Cuts at: 0 726 981
Size: 726 255
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI
HaeII KpnI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PpuMI PspOMI PspXI PstI SacI SacII SbfI
SfcI SmlI SphI StuI StyI TaqII TatI XcmI
XhoI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
BsaAI BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspHI
BsrBI BsrDI BssSI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EciI EcoNI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI PvuII RsrII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI
XmaI XmnI ZraI