Restriction map for
pF1KB4698
(Linear) (Six-base) MAPSORT of: KIBB4698.seq Check: 5333 from: 1 to: 2649
KIBB4698.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 659 883 2649
Size: 659 224 1766
Fragments arranged by size:
1766 659 224
Acc65I G'GTAC_C
Cuts at: 0 695 2649
Size: 695 1954
AclI AA'CG_TT
Cuts at: 0 2435 2649
Size: 2435 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 2649
Size: 944 1705
AfeI AGC'GCT
Cuts at: 0 1321 2649
Size: 1321 1328
AflIII A'CryG_T
Cuts at: 0 684 2649
Size: 684 1965
AhdI GACnn_n'nnGTC
Cuts at: 0 282 2649
Size: 282 2367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 542 2649
Size: 542 2107
ApaI G_GGCC'C
Cuts at: 0 1041 1148 2649
Size: 1041 107 1501
Fragments arranged by size:
1501 1041 107
ApoI r'AATT_y
Cuts at: 0 814 1436 1771 2583 2592 2649
Size: 814 622 335 812 9 57
Fragments arranged by size:
814 812 622 335 57 9
AvaI C'yCGr_G
Cuts at: 0 1555 2649
Size: 1555 1094
AvrII C'CTAG_G
Cuts at: 0 100 2649
Size: 100 2549
BamHI G'GATC_C
Cuts at: 0 2278 2649
Size: 2278 371
BanI G'GyrC_C
Cuts at: 0 106 695 1807 2649
Size: 106 589 1112 842
Fragments arranged by size:
1112 842 589 106
BanII G_rGCy'C
Cuts at: 0 405 864 1041 1133 1148 1205 2099 2649
Size: 405 459 177 92 15 57 894 550
Fragments arranged by size:
894 550 459 405 177 92 57 15
BbeI G_GCGC'C
Cuts at: 0 110 2649
Size: 110 2539
BbvCI CC'TCA_GC
Cuts at: 0 1970 2649
Size: 1970 679
BclI T'GATC_A
Cuts at: 0 1571 1892 2649
Size: 1571 321 757
Fragments arranged by size:
1571 757 321
BfrBI ATG'CAT
Cuts at: 0 15 2649
Size: 15 2634
BglII A'GATC_T
Cuts at: 0 591 1634 2044 2649
Size: 591 1043 410 605
Fragments arranged by size:
1043 605 591 410
Bme1580I G_kGCm'C
Cuts at: 0 1010 1041 1148 2421 2649
Size: 1010 31 107 1273 228
Fragments arranged by size:
1273 1010 228 107 31
BmgBI CAC'GTC
Cuts at: 0 324 2649
Size: 324 2325
BmrI ACTGGGnnnn_n'
Cuts at: 0 231 2290 2649
Size: 231 2059 359
Fragments arranged by size:
2059 359 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 878 1185 2462 2649
Size: 616 262 307 1277 187
Fragments arranged by size:
1277 616 307 262 187
Bpu10I CC'TnA_GC
Cuts at: 0 409 1970 2649
Size: 409 1561 679
Fragments arranged by size:
1561 679 409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1252 2649
Size: 1252 1397
BsaI GGTCTCn'nnnn_
Cuts at: 0 378 728 1069 2649
Size: 378 350 341 1580
Fragments arranged by size:
1580 378 350 341
BsaBI GATnn'nnATC
Cuts at: 0 1564 1897 2649
Size: 1564 333 752
Fragments arranged by size:
1564 752 333
BsaHI Gr'CG_yC
Cuts at: 0 107 2333 2649
Size: 107 2226 316
Fragments arranged by size:
2226 316 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1448 1478 2005 2035 2649
Size: 1448 30 527 30 614
Fragments arranged by size:
1448 614 527 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 263 1738 1893 2649
Size: 263 1475 155 756
Fragments arranged by size:
1475 756 263 155
BseYI C'CCAG_C
Cuts at: 0 340 999 2649
Size: 340 659 1650
Fragments arranged by size:
1650 659 340
BsiEI CG_ry'CG
Cuts at: 0 2123 2649
Size: 2123 526
BsiHKAI G_wGCw'C
Cuts at: 0 405 864 1133 1205 2649
Size: 405 459 269 72 1444
Fragments arranged by size:
1444 459 405 269 72
BsmI GAATG_Cn'
Cuts at: 0 981 2556 2649
Size: 981 1575 93
Fragments arranged by size:
1575 981 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 330 2649
Size: 330 2319
Bsp1286I G_dGCh'C
Cuts at: 0 405 864 1010 1041 1133 1148 1205 2099
Size: 405 459 146 31 92 15 57 894
Cuts at: 2099 2421 2649
Size: 322 228
Fragments arranged by size:
894 459 405 322 228 146 92 57
31 15
BspHI T'CATG_A
Cuts at: 0 1296 1547 1568 2649
Size: 1296 251 21 1081
Fragments arranged by size:
1296 1081 251 21
BsrDI GCAATG_nn'
Cuts at: 0 707 1320 2486 2649
Size: 707 613 1166 163
Fragments arranged by size:
1166 707 613 163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 489 2551 2649
Size: 489 2062 98
Fragments arranged by size:
2062 489 98
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1263 2649
Size: 1263 1386
BstYI r'GATC_y
Cuts at: 0 591 1634 1843 1974 2044 2278 2649
Size: 591 1043 209 131 70 234 371
Fragments arranged by size:
1043 591 371 234 209 131 70
BstZ17I GTA'TAC
Cuts at: 0 884 2649
Size: 884 1765
Bsu36I CC'TnA_GG
Cuts at: 0 940 2649
Size: 940 1709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2247 2649
Size: 2247 402
BtsI GCAGTG_nn'
Cuts at: 0 478 1010 2063 2649
Size: 478 532 1053 586
Fragments arranged by size:
1053 586 532 478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1350 1385 2468 2503 2649
Size: 1350 35 1083 35 146
Fragments arranged by size:
1350 1083 146 35 35
EaeI y'GGCC_r
Cuts at: 0 2120 2649
Size: 2120 529
EagI C'GGCC_G
Cuts at: 0 2120 2649
Size: 2120 529
EarI CTCTTCn'nnn_
Cuts at: 0 72 1514 1734 2649
Size: 72 1442 220 915
Fragments arranged by size:
1442 915 220 72
EcoICRI GAG'CTC
Cuts at: 0 403 862 1131 1203 2649
Size: 403 459 269 72 1446
Fragments arranged by size:
1446 459 403 269 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 878 944 1185 2462 2649
Size: 616 262 66 241 1277 187
Fragments arranged by size:
1277 616 262 241 187 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1045 2649
Size: 1045 1604
EcoO109I rG'GnC_Cy
Cuts at: 0 1037 1038 1145 2466 2649
Size: 1037 1 107 1321 183
Fragments arranged by size:
1321 1037 183 107 1
EcoRI G'AATT_C
Cuts at: 0 2583 2649
Size: 2583 66
EcoRV GAT'ATC
Cuts at: 0 1562 2649
Size: 1562 1087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2228 2649
Size: 2228 421
HaeII r_GCGC'y
Cuts at: 0 110 1323 2649
Size: 110 1213 1326
Fragments arranged by size:
1326 1213 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 377 409 1938 1970 2005 2037
Size: 268 32 77 32 1529 32 35 32
Cuts at: 2037 2649
Size: 612
Fragments arranged by size:
1529 612 268 77 35 32 32 32
32
HincII GTy'rAC
Cuts at: 0 833 1341 1388 2649
Size: 833 508 47 1261
Fragments arranged by size:
1261 833 508 47
KasI G'GCGC_C
Cuts at: 0 106 2649
Size: 106 2543
KpnI G_GTAC'C
Cuts at: 0 699 2649
Size: 699 1950
MfeI C'AATT_G
Cuts at: 0 1391 1487 2649
Size: 1391 96 1162
Fragments arranged by size:
1391 1162 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1434 1845 2153 2649
Size: 1434 411 308 496
Fragments arranged by size:
1434 496 411 308
MslI CAynn'nnrTG
Cuts at: 0 379 2649
Size: 379 2270
NarI GG'CG_CC
Cuts at: 0 107 2649
Size: 107 2542
NsiI A_TGCA'T
Cuts at: 0 17 2649
Size: 17 2632
NspI r_CATG'y
Cuts at: 0 2085 2649
Size: 2085 564
PasI CC'CwG_GG
Cuts at: 0 1010 2649
Size: 1010 1639
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 2649
Size: 355 2294
PsiI TTA'TAA
Cuts at: 0 2606 2649
Size: 2606 43
PspOMI G'GGCC_C
Cuts at: 0 1037 1144 2649
Size: 1037 107 1505
Fragments arranged by size:
1505 1037 107
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 128 160 2649
Size: 128 32 2489
Fragments arranged by size:
2489 128 32
PstI C_TGCA'G
Cuts at: 0 1353 2649
Size: 1353 1296
SacI G_AGCT'C
Cuts at: 0 405 864 1133 1205 2649
Size: 405 459 269 72 1444
Fragments arranged by size:
1444 459 405 269 72
SexAI A'CCwGG_T
Cuts at: 0 662 2649
Size: 662 1987
SfcI C'TryA_G
Cuts at: 0 1349 1937 2649
Size: 1349 588 712
Fragments arranged by size:
1349 712 588
SfoI GGC'GCC
Cuts at: 0 108 2649
Size: 108 2541
SmlI C'TyrA_G
Cuts at: 0 1231 2649
Size: 1231 1418
SspI AAT'ATT
Cuts at: 0 2562 2649
Size: 2562 87
StyI C'CwwG_G
Cuts at: 0 100 1802 2387 2469 2649
Size: 100 1702 585 82 180
Fragments arranged by size:
1702 585 180 100 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1795 2649
Size: 1795 854
TatI w'GTAC_w
Cuts at: 0 1379 2192 2649
Size: 1379 813 457
Fragments arranged by size:
1379 813 457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1007 2649
Size: 1007 1642
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AlfI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BclI BfrBI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI CspCI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII KasI KpnI MfeI MmeI
MslI NarI NsiI NspI PasI PflMI PsiI PspOMI
PsrI PstI SacI SexAI SfcI SfoI SmlI SspI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AflII AgeI AleI AloI AlwNI ApaLI
AscI AseI AsiSI BaeI BbsI BcgI BciVI BglI
BlpI BmtI BplI BsaAI BsaWI BsgI BsiWI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BtgI ClaI DraI DraIII DrdI EciI FseI FspI
FspAI HindIII HpaI MluI MscI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI