Restriction map for
pF1KB4699
(Linear) (Six-base) MAPSORT of: KIBB4699.seq Check: 7623 from: 1 to: 1110
KIBB4699.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 602 1110
Size: 602 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1110
Size: 519 591
AflIII A'CryG_T
Cuts at: 0 409 1110
Size: 409 701
AlwNI CAG_nnn'CTG
Cuts at: 0 970 1110
Size: 970 140
ApaI G_GGCC'C
Cuts at: 0 898 1110
Size: 898 212
BanII G_rGCy'C
Cuts at: 0 898 1110
Size: 898 212
BbvCI CC'TCA_GC
Cuts at: 0 1036 1110
Size: 1036 74
BglII A'GATC_T
Cuts at: 0 205 1110
Size: 205 905
Bme1580I G_kGCm'C
Cuts at: 0 484 898 1110
Size: 484 414 212
Fragments arranged by size:
484 414 212
BmgBI CAC'GTC
Cuts at: 0 410 477 1110
Size: 410 67 633
Fragments arranged by size:
633 410 67
BmtI G_CTAG'C
Cuts at: 0 733 1110
Size: 733 377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1110
Size: 719 391
Bpu10I CC'TnA_GC
Cuts at: 0 1036 1110
Size: 1036 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1110
Size: 592 518
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 1110
Size: 86 30 994
Fragments arranged by size:
994 86 30
BseYI C'CCAG_C
Cuts at: 0 768 1110
Size: 768 342
BsiEI CG_ry'CG
Cuts at: 0 633 1110
Size: 633 477
BsiHKAI G_wGCw'C
Cuts at: 0 162 1110
Size: 162 948
Bsp1286I G_dGCh'C
Cuts at: 0 162 484 898 1110
Size: 162 322 414 212
Fragments arranged by size:
414 322 212 162
BsrBI CCG'CTC
Cuts at: 0 530 1110
Size: 530 580
BstEII G'GTnAC_C
Cuts at: 0 1082 1110
Size: 1082 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 768 1110
Size: 768 342
BstYI r'GATC_y
Cuts at: 0 180 205 1110
Size: 180 25 905
Fragments arranged by size:
905 180 25
Bsu36I CC'TnA_GG
Cuts at: 0 552 1110
Size: 552 558
BtgI C'CryG_G
Cuts at: 0 452 1110
Size: 452 658
BtsI GCAGTG_nn'
Cuts at: 0 320 1110
Size: 320 790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 579 614 746 781 1110
Size: 579 35 132 35 329
Fragments arranged by size:
579 329 132 35 35
EaeI y'GGCC_r
Cuts at: 0 449 708 1110
Size: 449 259 402
Fragments arranged by size:
449 402 259
EarI CTCTTCn'nnn_
Cuts at: 0 340 1110
Size: 340 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 719 1110
Size: 519 200 391
Fragments arranged by size:
519 391 200
EcoO109I rG'GnC_Cy
Cuts at: 0 748 894 895 937 1110
Size: 748 146 1 42 173
Fragments arranged by size:
748 173 146 42 1
EcoRV GAT'ATC
Cuts at: 0 198 1110
Size: 198 912
HaeII r_GCGC'y
Cuts at: 0 1044 1110
Size: 1044 66
MfeI C'AATT_G
Cuts at: 0 723 1110
Size: 723 387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 546 1110
Size: 158 388 564
Fragments arranged by size:
564 388 158
MscI TGG'CCA
Cuts at: 0 451 710 1110
Size: 451 259 400
Fragments arranged by size:
451 400 259
MspA1I CmG'CkG
Cuts at: 0 22 718 967 1110
Size: 22 696 249 143
Fragments arranged by size:
696 249 143 22
NcoI C'CATG_G
Cuts at: 0 452 1110
Size: 452 658
NheI G'CTAG_C
Cuts at: 0 729 1110
Size: 729 381
PfoI T'CCnGG_A
Cuts at: 0 696 1110
Size: 696 414
PpuMI rG'GwC_Cy
Cuts at: 0 748 1110
Size: 748 362
PspOMI G'GGCC_C
Cuts at: 0 894 1110
Size: 894 216
PstI C_TGCA'G
Cuts at: 0 1072 1110
Size: 1072 38
PvuI CG_AT'CG
Cuts at: 0 633 1110
Size: 633 477
PvuII CAG'CTG
Cuts at: 0 22 718 967 1110
Size: 22 696 249 143
Fragments arranged by size:
696 249 143 22
SanDI GG'GwC_CC
Cuts at: 0 748 1110
Size: 748 362
SapI GCTCTTCn'nnn_
Cuts at: 0 340 1110
Size: 340 770
SexAI A'CCwGG_T
Cuts at: 0 902 1110
Size: 902 208
SfcI C'TryA_G
Cuts at: 0 9 941 1068 1110
Size: 9 932 127 42
Fragments arranged by size:
932 127 42 9
SmlI C'TyrA_G
Cuts at: 0 607 1110
Size: 607 503
StyI C'CwwG_G
Cuts at: 0 77 452 1087 1110
Size: 77 375 635 23
Fragments arranged by size:
635 375 77 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 774 1110
Size: 774 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 1110
Size: 86 32 992
Fragments arranged by size:
992 86 32
Tth111I GACn'n_nGTC
Cuts at: 0 441 1110
Size: 441 669
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI BanII BbvCI BglII
Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BsrBI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI EaeI EarI Eco57MI EcoO109I EcoRV
HaeII MfeI MmeI MscI MspA1I NcoI NheI PfoI
PpuMI PspOMI PstI PvuI PvuII SanDI SapI SexAI
SfcI SmlI StyI TaqII TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BlpI BmrI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MluI MslI NaeI NarI NdeI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XcmI XhoI XmaI XmnI ZraI