Restriction map for
pF1KB4700
(Linear) (Six-base) MAPSORT of: KIBB4700.seq Check: 8641 from: 1 to: 2718
KIBB4700.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 1033 1187 2718
Size: 1033 154 1531
Fragments arranged by size:
1531 1033 154
Acc65I G'GTAC_C
Cuts at: 0 1537 2718
Size: 1537 1181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 131 388 2718
Size: 40 91 257 2330
Fragments arranged by size:
2330 257 91 40
AfeI AGC'GCT
Cuts at: 0 20 2718
Size: 20 2698
AflIII A'CryG_T
Cuts at: 0 956 2696 2718
Size: 956 1740 22
Fragments arranged by size:
1740 956 22
AgeI A'CCGG_T
Cuts at: 0 2164 2718
Size: 2164 554
AleI CACnn'nnGTG
Cuts at: 0 2131 2718
Size: 2131 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1851 2718
Size: 1851 867
AlwNI CAG_nnn'CTG
Cuts at: 0 604 829 2718
Size: 604 225 1889
Fragments arranged by size:
1889 604 225
ApoI r'AATT_y
Cuts at: 0 1418 1742 2005 2718
Size: 1418 324 263 713
Fragments arranged by size:
1418 713 324 263
AseI AT'TA_AT
Cuts at: 0 725 1793 1862 2507 2718
Size: 725 1068 69 645 211
Fragments arranged by size:
1068 725 645 211 69
AvaI C'yCGr_G
Cuts at: 0 645 2718
Size: 645 2073
BanI G'GyrC_C
Cuts at: 0 1537 2161 2167 2718
Size: 1537 624 6 551
Fragments arranged by size:
1537 624 551 6
BanII G_rGCy'C
Cuts at: 0 2453 2614 2718
Size: 2453 161 104
Fragments arranged by size:
2453 161 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 2348 2718
Size: 2348 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 116 126 150 160 874 908 2718
Size: 116 10 24 10 714 34 1810
Fragments arranged by size:
1810 714 116 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 2468 2718
Size: 2468 250
BglII A'GATC_T
Cuts at: 0 1775 2718
Size: 1775 943
BlpI GC'TnA_GC
Cuts at: 0 1985 2718
Size: 1985 733
Bme1580I G_kGCm'C
Cuts at: 0 711 2718
Size: 711 2007
BmrI ACTGGGnnnn_n'
Cuts at: 0 489 602 2448 2676 2718
Size: 489 113 1846 228 42
Fragments arranged by size:
1846 489 228 113 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 1121 1829 1916 2507 2718
Size: 498 623 708 87 591 211
Fragments arranged by size:
708 623 591 498 211 87
Bpu10I CC'TnA_GC
Cuts at: 0 1562 2718
Size: 1562 1156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1793 1805 2379 2610 2628 2718
Size: 515 1278 12 574 231 18 90
Fragments arranged by size:
1278 574 515 231 90 18 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 322 2718
Size: 322 2396
BsaBI GATnn'nnATC
Cuts at: 0 1223 2718
Size: 1223 1495
BsaHI Gr'CG_yC
Cuts at: 0 61 2718
Size: 61 2657
BsaWI w'CCGG_w
Cuts at: 0 2164 2460 2718
Size: 2164 296 258
Fragments arranged by size:
2164 296 258
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 2348 2718
Size: 400 1948 370
Fragments arranged by size:
1948 400 370
BseYI C'CCAG_C
Cuts at: 0 2599 2718
Size: 2599 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 2109 2718
Size: 146 1963 609
Fragments arranged by size:
1963 609 146
BsiEI CG_ry'CG
Cuts at: 0 1103 2718
Size: 1103 1615
BsiHKAI G_wGCw'C
Cuts at: 0 2614 2718
Size: 2614 104
Bsp1286I G_dGCh'C
Cuts at: 0 711 2453 2614 2718
Size: 711 1742 161 104
Fragments arranged by size:
1742 711 161 104
BsrDI GCAATG_nn'
Cuts at: 0 195 2174 2718
Size: 195 1979 544
Fragments arranged by size:
1979 544 195
BsrFI r'CCGG_y
Cuts at: 0 1534 2164 2718
Size: 1534 630 554
Fragments arranged by size:
1534 630 554
BsrGI T'GTAC_A
Cuts at: 0 1256 2718
Size: 1256 1462
BstAPI GCAn_nnn'nTGC
Cuts at: 0 877 2718
Size: 877 1841
BstYI r'GATC_y
Cuts at: 0 1275 1638 1775 2718
Size: 1275 363 137 943
Fragments arranged by size:
1275 943 363 137
BstZ17I GTA'TAC
Cuts at: 0 1034 2718
Size: 1034 1684
Bsu36I CC'TnA_GG
Cuts at: 0 830 2718
Size: 830 1888
BtgI C'CryG_G
Cuts at: 0 1097 1334 2674 2718
Size: 1097 237 1340 44
Fragments arranged by size:
1340 1097 237 44
BtsI GCAGTG_nn'
Cuts at: 0 225 574 1259 1556 2592 2594 2718
Size: 225 349 685 297 1036 2 124
Fragments arranged by size:
1036 685 349 297 225 124 2
ClaI AT'CG_AT
Cuts at: 0 1575 2718
Size: 1575 1143
DraI TTT'AAA
Cuts at: 0 1997 2312 2718
Size: 1997 315 406
Fragments arranged by size:
1997 406 315
DrdI GACnn_nn'nnGTC
Cuts at: 0 1327 2718
Size: 1327 1391
EaeI y'GGCC_r
Cuts at: 0 453 1100 2718
Size: 453 647 1618
Fragments arranged by size:
1618 647 453
EagI C'GGCC_G
Cuts at: 0 1100 2718
Size: 1100 1618
EarI CTCTTCn'nnn_
Cuts at: 0 167 291 466 2718
Size: 167 124 175 2252
Fragments arranged by size:
2252 175 167 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1286 2718
Size: 1286 1432
EcoICRI GAG'CTC
Cuts at: 0 2612 2718
Size: 2612 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 131 388 498 1121 1829 1916 2507
Size: 40 91 257 110 623 708 87 591
Cuts at: 2507 2718
Size: 211
Fragments arranged by size:
708 623 591 257 211 110 91 87
40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 2718
Size: 55 2663
EcoO109I rG'GnC_Cy
Cuts at: 0 520 1566 2514 2603 2718
Size: 520 1046 948 89 115
Fragments arranged by size:
1046 948 520 115 89
EcoRI G'AATT_C
Cuts at: 0 1418 1742 2718
Size: 1418 324 976
Fragments arranged by size:
1418 976 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 393 962 1543 2718
Size: 393 569 581 1175
Fragments arranged by size:
1175 581 569 393
HaeII r_GCGC'y
Cuts at: 0 22 2718
Size: 22 2696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 467 499 1669 1701 1947 1979 2718
Size: 467 32 1170 32 246 32 739
Fragments arranged by size:
1170 739 467 246 32 32 32
HincII GTy'rAC
Cuts at: 0 1439 1547 2718
Size: 1439 108 1171
Fragments arranged by size:
1439 1171 108
HindIII A'AGCT_T
Cuts at: 0 273 2718
Size: 273 2445
KpnI G_GTAC'C
Cuts at: 0 1541 2718
Size: 1541 1177
MfeI C'AATT_G
Cuts at: 0 872 1623 1664 2718
Size: 872 751 41 1054
Fragments arranged by size:
1054 872 751 41
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 2138 2442 2718
Size: 640 1498 304 276
Fragments arranged by size:
1498 640 304 276
MscI TGG'CCA
Cuts at: 0 455 2718
Size: 455 2263
MslI CAynn'nnrTG
Cuts at: 0 994 1111 2131 2718
Size: 994 117 1020 587
Fragments arranged by size:
1020 994 587 117
MspA1I CmG'CkG
Cuts at: 0 39 654 1003 2367 2718
Size: 39 615 349 1364 351
Fragments arranged by size:
1364 615 351 349 39
NdeI CA'TA_TG
Cuts at: 0 852 1077 2553 2718
Size: 852 225 1476 165
Fragments arranged by size:
1476 852 225 165
NruI TCG'CGA
Cuts at: 0 680 2718
Size: 680 2038
NspI r_CATG'y
Cuts at: 0 298 715 960 2700 2718
Size: 298 417 245 1740 18
Fragments arranged by size:
1740 417 298 245 18
PciI A'CATG_T
Cuts at: 0 956 2696 2718
Size: 956 1740 22
Fragments arranged by size:
1740 956 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 1900 2485 2718
Size: 1900 585 233
Fragments arranged by size:
1900 585 233
PpuMI rG'GwC_Cy
Cuts at: 0 520 1566 2603 2718
Size: 520 1046 1037 115
Fragments arranged by size:
1046 1037 520 115
PshAI GACnn'nnGTC
Cuts at: 0 1120 2718
Size: 1120 1598
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 2718
Size: 1240 32 1446
Fragments arranged by size:
1446 1240 32
PstI C_TGCA'G
Cuts at: 0 231 1392 1775 2372 2475 2718
Size: 231 1161 383 597 103 243
Fragments arranged by size:
1161 597 383 243 231 103
PvuII CAG'CTG
Cuts at: 0 654 1003 2367 2718
Size: 654 349 1364 351
Fragments arranged by size:
1364 654 351 349
RsrII CG'GwC_CG
Cuts at: 0 2463 2718
Size: 2463 255
SacI G_AGCT'C
Cuts at: 0 2614 2718
Size: 2614 104
SexAI A'CCwGG_T
Cuts at: 0 806 2718
Size: 806 1912
SfcI C'TryA_G
Cuts at: 0 65 227 1052 1388 1771 2368 2471 2718
Size: 65 162 825 336 383 597 103 247
Fragments arranged by size:
825 597 383 336 247 162 103 65
SgrAI Cr'CCGG_yG
Cuts at: 0 2164 2718
Size: 2164 554
SmlI C'TyrA_G
Cuts at: 0 530 1808 1820 2358 2607 2625 2718
Size: 530 1278 12 538 249 18 93
Fragments arranged by size:
1278 538 530 249 93 18 12
SphI G_CATG'C
Cuts at: 0 298 2718
Size: 298 2420
SspI AAT'ATT
Cuts at: 0 1593 1866 2076 2307 2718
Size: 1593 273 210 231 411
Fragments arranged by size:
1593 411 273 231 210
StyI C'CwwG_G
Cuts at: 0 1412 2718
Size: 1412 1306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 966 1231 1318 1694 2137 2718
Size: 966 265 87 376 443 581
Fragments arranged by size:
966 581 443 376 265 87
TatI w'GTAC_w
Cuts at: 0 204 1256 2718
Size: 204 1052 1462
Fragments arranged by size:
1462 1052 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 722 754 839 871 2133 2165 2718
Size: 722 32 85 32 1262 32 553
Fragments arranged by size:
1262 722 553 85 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 481 757 2718
Size: 481 276 1961
Fragments arranged by size:
1961 481 276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 616 814 2406 2718
Size: 616 198 1592 312
Fragments arranged by size:
1592 616 312 198
XmnI GAAnn'nnTTC
Cuts at: 0 1526 2718
Size: 1526 1192
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApoI AseI AvaI BanI BanII BbsI BcgI
BciVI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BsrDI BsrFI BsrGI BstAPI BstYI BstZ17I
Bsu36I BtgI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI MspA1I NdeI NruI NspI PciI PflMI PpuMI
PshAI PsrI PstI PvuII RsrII SacI SexAI SfcI
SgrAI SmlI SphI SspI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AhdI AloI ApaI ApaLI
AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BclI
BfrBI BglI BmgBI BmtI BplI BsaAI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI
BstBI BstEII BstXI BtgZI CspCI DraIII EcoRV FseI
FspI FspAI HpaI KasI MluI NaeI NarI NcoI
NgoMIV NheI NotI NsiI PacI PasI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PvuI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SmaI
SnaBI SpeI SrfI StuI SwaI XbaI XhoI XmaI
ZraI