Restriction map for
pF1KB4702
(Linear) (Six-base) MAPSORT of: KIBB4702.seq Check: 673 from: 1 to: 1722
KIBB4702.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 38 1722
Size: 38 1684
AflIII A'CryG_T
Cuts at: 0 825 1722
Size: 825 897
AleI CACnn'nnGTG
Cuts at: 0 600 1722
Size: 600 1122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 228 1722
Size: 228 1494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 1722
Size: 116 32 1574
Fragments arranged by size:
1574 116 32
AlwNI CAG_nnn'CTG
Cuts at: 0 838 1722
Size: 838 884
ApoI r'AATT_y
Cuts at: 0 273 1211 1282 1345 1722
Size: 273 938 71 63 377
Fragments arranged by size:
938 377 273 71 63
AvrII C'CTAG_G
Cuts at: 0 240 1722
Size: 240 1482
BglI GCCn_nnn'nGGC
Cuts at: 0 53 1722
Size: 53 1669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 747 1722
Size: 747 975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1574 1722
Size: 380 1194 148
Fragments arranged by size:
1194 380 148
Bpu10I CC'TnA_GC
Cuts at: 0 961 1722
Size: 961 761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1400 1722
Size: 437 963 322
Fragments arranged by size:
963 437 322
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 20 50 116 146 1577 1607 1722
Size: 20 30 66 30 1431 30 115
Fragments arranged by size:
1431 115 66 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 794 1722
Size: 794 928
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1722
Size: 784 938
BsiHKAI G_wGCw'C
Cuts at: 0 442 535 1560 1722
Size: 442 93 1025 162
Fragments arranged by size:
1025 442 162 93
BsmI GAATG_Cn'
Cuts at: 0 645 852 1722
Size: 645 207 870
Fragments arranged by size:
870 645 207
Bsp1286I G_dGCh'C
Cuts at: 0 442 535 1560 1722
Size: 442 93 1025 162
Fragments arranged by size:
1025 442 162 93
BspHI T'CATG_A
Cuts at: 0 168 516 1722
Size: 168 348 1206
Fragments arranged by size:
1206 348 168
BsrBI CCG'CTC
Cuts at: 0 31 1722
Size: 31 1691
BsrDI GCAATG_nn'
Cuts at: 0 1096 1722
Size: 1096 626
BsrFI r'CCGG_y
Cuts at: 0 47 1722
Size: 47 1675
BstAPI GCAn_nnn'nTGC
Cuts at: 0 546 847 1722
Size: 546 301 875
Fragments arranged by size:
875 546 301
BstYI r'GATC_y
Cuts at: 0 674 1722
Size: 674 1048
BtsI GCAGTG_nn'
Cuts at: 0 702 1722
Size: 702 1020
DraIII CAC_nnn'GTG
Cuts at: 0 422 546 1722
Size: 422 124 1176
Fragments arranged by size:
1176 422 124
EaeI y'GGCC_r
Cuts at: 0 45 153 1611 1722
Size: 45 108 1458 111
Fragments arranged by size:
1458 111 108 45
EarI CTCTTCn'nnn_
Cuts at: 0 195 197 1722
Size: 195 2 1525
Fragments arranged by size:
1525 195 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1574 1722
Size: 380 1194 148
Fragments arranged by size:
1194 380 148
EcoRI G'AATT_C
Cuts at: 0 1282 1722
Size: 1282 440
EcoRV GAT'ATC
Cuts at: 0 356 1722
Size: 356 1366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 415 1722
Size: 415 1307
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 773 805 1666 1698 1722
Size: 353 32 388 32 861 32 24
Fragments arranged by size:
861 388 353 32 32 32 24
HincII GTy'rAC
Cuts at: 0 1493 1722
Size: 1493 229
HpaI GTT'AAC
Cuts at: 0 1493 1722
Size: 1493 229
MfeI C'AATT_G
Cuts at: 0 1304 1722
Size: 1304 418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 1127 1324 1652 1722
Size: 735 392 197 328 70
Fragments arranged by size:
735 392 328 197 70
MscI TGG'CCA
Cuts at: 0 155 1722
Size: 155 1567
MslI CAynn'nnrTG
Cuts at: 0 600 941 1722
Size: 600 341 781
Fragments arranged by size:
781 600 341
MspA1I CmG'CkG
Cuts at: 0 70 1722
Size: 70 1652
NaeI GCC'GGC
Cuts at: 0 49 1722
Size: 49 1673
NgoMIV G'CCGG_C
Cuts at: 0 47 1722
Size: 47 1675
NspI r_CATG'y
Cuts at: 0 553 829 1722
Size: 553 276 893
Fragments arranged by size:
893 553 276
PciI A'CATG_T
Cuts at: 0 825 1722
Size: 825 897
PflMI CCAn_nnn'nTGG
Cuts at: 0 1306 1722
Size: 1306 416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 1722
Size: 116 32 1574
Fragments arranged by size:
1574 116 32
PstI C_TGCA'G
Cuts at: 0 308 339 843 1011 1722
Size: 308 31 504 168 711
Fragments arranged by size:
711 504 308 168 31
PvuII CAG'CTG
Cuts at: 0 70 1722
Size: 70 1652
SapI GCTCTTCn'nnn_
Cuts at: 0 195 1722
Size: 195 1527
SexAI A'CCwGG_T
Cuts at: 0 542 1722
Size: 542 1180
SfcI C'TryA_G
Cuts at: 0 71 304 335 839 1007 1325 1722
Size: 71 233 31 504 168 318 397
Fragments arranged by size:
504 397 318 233 168 71 31
SmlI C'TyrA_G
Cuts at: 0 452 1415 1722
Size: 452 963 307
Fragments arranged by size:
963 452 307
SphI G_CATG'C
Cuts at: 0 553 1722
Size: 553 1169
StyI C'CwwG_G
Cuts at: 0 240 1722
Size: 240 1482
Enzymes that do cut:
AccI AflIII AleI AlfI AloI AlwNI ApoI AvrII
BglI BplI BpmI Bpu10I BpuEI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI
BstYI BtsI DraIII EaeI EarI Eco57MI EcoRI EcoRV
FalI Hin4I HincII HpaI MfeI MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PciI PflMI PpiI PstI
PvuII SapI SexAI SfcI SmlI SphI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI
BsmBI BspEI BspMI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI
FspAI HaeII HindIII KasI KpnI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI