Restriction map for
pF1KB4707
(Linear) (Six-base) MAPSORT of: KIBB4707.seq Check: 4509 from: 1 to: 1086
KIBB4707.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 179 1086
Size: 179 907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 590 943 1086
Size: 571 19 353 143
Fragments arranged by size:
571 353 143 19
AflIII A'CryG_T
Cuts at: 0 414 1086
Size: 414 672
AleI CACnn'nnGTG
Cuts at: 0 28 507 1086
Size: 28 479 579
Fragments arranged by size:
579 479 28
AlwNI CAG_nnn'CTG
Cuts at: 0 276 1086
Size: 276 810
AvaI C'yCGr_G
Cuts at: 0 429 1086
Size: 429 657
BanII G_rGCy'C
Cuts at: 0 362 650 1086
Size: 362 288 436
Fragments arranged by size:
436 362 288
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 1086
Size: 211 875
BbvCI CC'TCA_GC
Cuts at: 0 630 1086
Size: 630 456
BciVI GTATCCnnnnn_n'
Cuts at: 0 625 1086
Size: 625 461
BfrBI ATG'CAT
Cuts at: 0 124 1086
Size: 124 962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1086
Size: 664 422
Bpu10I CC'TnA_GC
Cuts at: 0 630 1086
Size: 630 456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 821 977 1086
Size: 120 701 156 109
Fragments arranged by size:
701 156 120 109
BsaI GGTCTCn'nnnn_
Cuts at: 0 813 1086
Size: 813 273
BsaAI yAC'GTr
Cuts at: 0 415 1086
Size: 415 671
BsaHI Gr'CG_yC
Cuts at: 0 176 1086
Size: 176 910
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 905 1086
Size: 772 133 181
Fragments arranged by size:
772 181 133
BsiHKAI G_wGCw'C
Cuts at: 0 650 1086
Size: 650 436
BsmI GAATG_Cn'
Cuts at: 0 837 1086
Size: 837 249
Bsp1286I G_dGCh'C
Cuts at: 0 362 650 1086
Size: 362 288 436
Fragments arranged by size:
436 362 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 831 1086
Size: 831 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 642 1086
Size: 642 444
BstYI r'GATC_y
Cuts at: 0 1030 1086
Size: 1030 56
BtgI C'CryG_G
Cuts at: 0 354 674 1086
Size: 354 320 412
Fragments arranged by size:
412 354 320
DrdI GACnn_nn'nnGTC
Cuts at: 0 442 1086
Size: 442 644
EarI CTCTTCn'nnn_
Cuts at: 0 725 1086
Size: 725 361
EcoICRI GAG'CTC
Cuts at: 0 648 1086
Size: 648 438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 590 664 943 1086
Size: 571 19 74 279 143
Fragments arranged by size:
571 279 143 74 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 892 924 973 1005 1015 1047
Size: 510 32 350 32 49 32 10 32
Cuts at: 1047 1086
Size: 39
Fragments arranged by size:
510 350 49 39 32 32 32 32
10
HindIII A'AGCT_T
Cuts at: 0 138 1086
Size: 138 948
MfeI C'AATT_G
Cuts at: 0 209 1086
Size: 209 877
MslI CAynn'nnrTG
Cuts at: 0 28 167 507 1086
Size: 28 139 340 579
Fragments arranged by size:
579 340 139 28
MspA1I CmG'CkG
Cuts at: 0 52 1083 1086
Size: 52 1031 3
Fragments arranged by size:
1031 52 3
NcoI C'CATG_G
Cuts at: 0 674 1086
Size: 674 412
NdeI CA'TA_TG
Cuts at: 0 1068 1086
Size: 1068 18
NsiI A_TGCA'T
Cuts at: 0 126 1086
Size: 126 960
PstI C_TGCA'G
Cuts at: 0 1038 1086
Size: 1038 48
PvuII CAG'CTG
Cuts at: 0 52 1083 1086
Size: 52 1031 3
Fragments arranged by size:
1031 52 3
SacI G_AGCT'C
Cuts at: 0 650 1086
Size: 650 436
SfcI C'TryA_G
Cuts at: 0 1034 1086
Size: 1034 52
SmlI C'TyrA_G
Cuts at: 0 135 836 992 1086
Size: 135 701 156 94
Fragments arranged by size:
701 156 135 94
StuI AGG'CCT
Cuts at: 0 595 1086
Size: 595 491
StyI C'CwwG_G
Cuts at: 0 674 1086
Size: 674 412
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1086
Size: 790 296
ZraI GAC'GTC
Cuts at: 0 177 1086
Size: 177 909
Enzymes that do cut:
AatII AcuI AflIII AleI AlwNI AvaI BanII BbsI
BbvCI BciVI BfrBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BseRI BsiHKAI BsmI Bsp1286I BspMI BstXI BstYI
BtgI DrdI EarI EcoICRI Eco57MI Hin4I HindIII MfeI
MslI MspA1I NcoI NdeI NsiI PstI PvuII SacI
SfcI SmlI StuI StyI TaqII ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BsaBI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI MmeI MscI NaeI NarI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI