Restriction map for
pF1KB4708
(Linear) (Six-base) MAPSORT of: KIBB4708.seq Check: 7194 from: 1 to: 1332
KIBB4708.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1096 1332
Size: 1096 236
AccI GT'mk_AC
Cuts at: 0 560 1332
Size: 560 772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1309 1332
Size: 45 1264 23
Fragments arranged by size:
1264 45 23
AflIII A'CryG_T
Cuts at: 0 389 710 1332
Size: 389 321 622
Fragments arranged by size:
622 389 321
ApaI G_GGCC'C
Cuts at: 0 345 1266 1332
Size: 345 921 66
Fragments arranged by size:
921 345 66
ApoI r'AATT_y
Cuts at: 0 238 595 1332
Size: 238 357 737
Fragments arranged by size:
737 357 238
AvaI C'yCGr_G
Cuts at: 0 159 1332
Size: 159 1173
BamHI G'GATC_C
Cuts at: 0 687 1332
Size: 687 645
BanI G'GyrC_C
Cuts at: 0 51 619 1332
Size: 51 568 713
Fragments arranged by size:
713 568 51
BanII G_rGCy'C
Cuts at: 0 296 345 957 1266 1332
Size: 296 49 612 309 66
Fragments arranged by size:
612 309 296 66 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 358 1332
Size: 358 974
BbvCI CC'TCA_GC
Cuts at: 0 359 1332
Size: 359 973
Bme1580I G_kGCm'C
Cuts at: 0 56 185 345 1266 1332
Size: 56 129 160 921 66
Fragments arranged by size:
921 160 129 66 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 885 1332
Size: 885 447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 448 1006 1332
Size: 212 236 558 326
Fragments arranged by size:
558 326 236 212
Bpu10I CC'TnA_GC
Cuts at: 0 359 1332
Size: 359 973
BsaAI yAC'GTr
Cuts at: 0 390 711 1332
Size: 390 321 621
Fragments arranged by size:
621 390 321
BsaHI Gr'CG_yC
Cuts at: 0 1316 1332
Size: 1316 16
BsaWI w'CCGG_w
Cuts at: 0 911 1332
Size: 911 421
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 304 391 394 1332
Size: 85 219 87 3 938
Fragments arranged by size:
938 219 87 85 3
BseYI C'CCAG_C
Cuts at: 0 65 295 1332
Size: 65 230 1037
Fragments arranged by size:
1037 230 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 1332
Size: 691 641
BsiHKAI G_wGCw'C
Cuts at: 0 1154 1332
Size: 1154 178
Bsp1286I G_dGCh'C
Cuts at: 0 56 185 296 345 957 1154 1266 1332
Size: 56 129 111 49 612 197 112 66
Fragments arranged by size:
612 197 129 112 111 66 56 49
BspHI T'CATG_A
Cuts at: 0 851 1332
Size: 851 481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 776 1096 1332
Size: 98 678 320 236
Fragments arranged by size:
678 320 236 98
BsrDI GCAATG_nn'
Cuts at: 0 434 471 1332
Size: 434 37 861
Fragments arranged by size:
861 434 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 208 1332
Size: 90 118 1124
Fragments arranged by size:
1124 118 90
BstYI r'GATC_y
Cuts at: 0 687 1332
Size: 687 645
Bsu36I CC'TnA_GG
Cuts at: 0 346 1332
Size: 346 986
BtgI C'CryG_G
Cuts at: 0 306 1332
Size: 306 1026
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 405 628 1332
Size: 405 223 704
Fragments arranged by size:
704 405 223
EaeI y'GGCC_r
Cuts at: 0 546 606 1332
Size: 546 60 726
Fragments arranged by size:
726 546 60
EarI CTCTTCn'nnn_
Cuts at: 0 1165 1332
Size: 1165 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 212 448 1006 1309 1332
Size: 45 167 236 558 303 23
Fragments arranged by size:
558 303 236 167 45 23
EcoO109I rG'GnC_Cy
Cuts at: 0 342 1262 1263 1283 1332
Size: 342 920 1 20 49
Fragments arranged by size:
920 342 49 20 1
EcoRI G'AATT_C
Cuts at: 0 238 1332
Size: 238 1094
FspI TGC'GCA
Cuts at: 0 94 1332
Size: 94 1238
MscI TGG'CCA
Cuts at: 0 548 608 1332
Size: 548 60 724
Fragments arranged by size:
724 548 60
MslI CAynn'nnrTG
Cuts at: 0 542 826 856 1015 1030 1332
Size: 542 284 30 159 15 302
Fragments arranged by size:
542 302 284 159 30 15
MspA1I CmG'CkG
Cuts at: 0 65 171 816 1077 1203 1332
Size: 65 106 645 261 126 129
Fragments arranged by size:
645 261 129 126 106 65
NspI r_CATG'y
Cuts at: 0 705 1332
Size: 705 627
PflMI CCAn_nnn'nTGG
Cuts at: 0 635 1332
Size: 635 697
PfoI T'CCnGG_A
Cuts at: 0 983 1332
Size: 983 349
PmlI CAC'GTG
Cuts at: 0 390 711 1332
Size: 390 321 621
Fragments arranged by size:
621 390 321
PpuMI rG'GwC_Cy
Cuts at: 0 1283 1332
Size: 1283 49
PspOMI G'GGCC_C
Cuts at: 0 341 1262 1332
Size: 341 921 70
Fragments arranged by size:
921 341 70
PvuII CAG'CTG
Cuts at: 0 65 1077 1332
Size: 65 1012 255
Fragments arranged by size:
1012 255 65
SfcI C'TryA_G
Cuts at: 0 561 1332
Size: 561 771
SmaI CCC'GGG
Cuts at: 0 161 1332
Size: 161 1171
StuI AGG'CCT
Cuts at: 0 1051 1332
Size: 1051 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 867 1332
Size: 867 465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1092 1332
Size: 1092 240
XmaI C'CCGG_G
Cuts at: 0 159 1332
Size: 159 1173
XmnI GAAnn'nnTTC
Cuts at: 0 373 1332
Size: 373 959
Enzymes that do cut:
AarI AccI AcuI AflIII ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI Bme1580I BmrI BpmI Bpu10I
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BstAPI BstYI Bsu36I BtgI BtgZI
EaeI EarI Eco57MI EcoO109I EcoRI FspI MscI MslI
MspA1I NspI PflMI PfoI PmlI PpuMI PspOMI PvuII
SfcI SmaI StuI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
TstI Tth111I XbaI XhoI ZraI