Restriction map for
pF1KB4714
(Linear) (Six-base) MAPSORT of: KIBB4714.seq Check: 9243 from: 1 to: 2622
KIBB4714.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 503 1456 2553 2622
Size: 503 953 1097 69
Fragments arranged by size:
1097 953 503 69
AccI GT'mk_AC
Cuts at: 0 2374 2597 2622
Size: 2374 223 25
Fragments arranged by size:
2374 223 25
AclI AA'CG_TT
Cuts at: 0 2362 2622
Size: 2362 260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1558 2622
Size: 520 1038 1064
Fragments arranged by size:
1064 1038 520
AfeI AGC'GCT
Cuts at: 0 424 526 2622
Size: 424 102 2096
Fragments arranged by size:
2096 424 102
AflII C'TTAA_G
Cuts at: 0 667 1045 2622
Size: 667 378 1577
Fragments arranged by size:
1577 667 378
AflIII A'CryG_T
Cuts at: 0 2477 2622
Size: 2477 145
AhdI GACnn_n'nnGTC
Cuts at: 0 1728 2622
Size: 1728 894
AleI CACnn'nnGTG
Cuts at: 0 1314 2125 2155 2435 2622
Size: 1314 811 30 280 187
Fragments arranged by size:
1314 811 280 187 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 2622
Size: 424 2198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1324 1356 2347 2379 2622
Size: 1324 32 991 32 243
Fragments arranged by size:
1324 991 243 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1471 2622
Size: 1471 1151
ApaI G_GGCC'C
Cuts at: 0 367 1391 1883 2622
Size: 367 1024 492 739
Fragments arranged by size:
1024 739 492 367
ApoI r'AATT_y
Cuts at: 0 10 1120 2344 2622
Size: 10 1110 1224 278
Fragments arranged by size:
1224 1110 278 10
AvaI C'yCGr_G
Cuts at: 0 2107 2622
Size: 2107 515
BanI G'GyrC_C
Cuts at: 0 453 1749 2622
Size: 453 1296 873
Fragments arranged by size:
1296 873 453
BanII G_rGCy'C
Cuts at: 0 367 381 596 1391 1500 1683 1883 1907
Size: 367 14 215 795 109 183 200 24
Cuts at: 1907 2048 2622
Size: 141 574
Fragments arranged by size:
795 574 367 215 200 183 141 109
24 14
BbeI G_GCGC'C
Cuts at: 0 1753 2622
Size: 1753 869
BbsI GAAGACnn'nnnn_
Cuts at: 0 356 476 2150 2622
Size: 356 120 1674 472
Fragments arranged by size:
1674 472 356 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 399 2622
Size: 365 34 2223
Fragments arranged by size:
2223 365 34
BclI T'GATC_A
Cuts at: 0 641 2283 2622
Size: 641 1642 339
Fragments arranged by size:
1642 641 339
BglI GCCn_nnn'nGGC
Cuts at: 0 814 1027 2622
Size: 814 213 1595
Fragments arranged by size:
1595 814 213
BglII A'GATC_T
Cuts at: 0 1088 1832 2262 2622
Size: 1088 744 430 360
Fragments arranged by size:
1088 744 430 360
Bme1580I G_kGCm'C
Cuts at: 0 135 367 458 1155 1391 1739 1883 2622
Size: 135 232 91 697 236 348 144 739
Fragments arranged by size:
739 697 348 236 232 144 135 91
BmgBI CAC'GTC
Cuts at: 0 516 2622
Size: 516 2106
BmrI ACTGGGnnnn_n'
Cuts at: 0 350 452 1514 2225 2622
Size: 350 102 1062 711 397
Fragments arranged by size:
1062 711 397 350 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 450 859 1006 1624 1693 2622
Size: 67 383 409 147 618 69 929
Fragments arranged by size:
929 618 409 383 147 69 67
Bpu10I CC'TnA_GC
Cuts at: 0 542 1901 2622
Size: 542 1359 721
Fragments arranged by size:
1359 721 542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1711 2622
Size: 85 1626 911
Fragments arranged by size:
1626 911 85
BsaAI yAC'GTr
Cuts at: 0 2076 2622
Size: 2076 546
BsaHI Gr'CG_yC
Cuts at: 0 1750 2622
Size: 1750 872
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 578 608 2347 2377 2622
Size: 578 30 1739 30 245
Fragments arranged by size:
1739 578 245 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 739 1083 1468 2599 2622
Size: 43 696 344 385 1131 23
Fragments arranged by size:
1131 696 385 344 43 23
BseYI C'CCAG_C
Cuts at: 0 527 2622
Size: 527 2095
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1932 1977 2056 2148 2622
Size: 525 1407 45 79 92 474
Fragments arranged by size:
1407 525 474 92 79 45
BsiEI CG_ry'CG
Cuts at: 0 200 371 1074 2622
Size: 200 171 703 1548
Fragments arranged by size:
1548 703 200 171
BsiHKAI G_wGCw'C
Cuts at: 0 179 875 1482 2069 2622
Size: 179 696 607 587 553
Fragments arranged by size:
696 607 587 553 179
BsmI GAATG_Cn'
Cuts at: 0 2432 2622
Size: 2432 190
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1921 2622
Size: 1921 701
Bsp1286I G_dGCh'C
Cuts at: 0 135 179 367 381 458 596 875 1155
Size: 135 44 188 14 77 138 279 280
Cuts at: 1155 1391 1482 1500 1683 1739 1883 1907 2048
Size: 236 91 18 183 56 144 24 141
Cuts at: 2048 2069 2622
Size: 21 553
Fragments arranged by size:
553 280 279 236 188 183 144 141
138 135 91 77 56 44 24 21
18 14
BspHI T'CATG_A
Cuts at: 0 1079 2300 2511 2622
Size: 1079 1221 211 111
Fragments arranged by size:
1221 1079 211 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 1456 2122 2553 2622
Size: 503 953 666 431 69
Fragments arranged by size:
953 666 503 431 69
BsrDI GCAATG_nn'
Cuts at: 0 290 1016 2622
Size: 290 726 1606
Fragments arranged by size:
1606 726 290
BssHII G'CGCG_C
Cuts at: 0 1242 2622
Size: 1242 1380
BssSI C'ACGA_G
Cuts at: 0 478 787 2622
Size: 478 309 1835
Fragments arranged by size:
1835 478 309
BstBI TT'CG_AA
Cuts at: 0 2315 2622
Size: 2315 307
BstXI CCAn_nnnn'nTGG
Cuts at: 0 852 1527 1974 2157 2580 2622
Size: 852 675 447 183 423 42
Fragments arranged by size:
852 675 447 423 183 42
BstYI r'GATC_y
Cuts at: 0 981 1088 1832 2262 2622
Size: 981 107 744 430 360
Fragments arranged by size:
981 744 430 360 107
BtgI C'CryG_G
Cuts at: 0 188 1745 1754 1869 1907 2622
Size: 188 1557 9 115 38 715
Fragments arranged by size:
1557 715 188 115 38 9
BtsI GCAGTG_nn'
Cuts at: 0 846 1862 2131 2622
Size: 846 1016 269 491
Fragments arranged by size:
1016 846 491 269
DrdI GACnn_nn'nnGTC
Cuts at: 0 1305 2622
Size: 1305 1317
EaeI y'GGCC_r
Cuts at: 0 368 704 815 1028 1352 1742 2622
Size: 368 336 111 213 324 390 880
Fragments arranged by size:
880 390 368 336 324 213 111
EagI C'GGCC_G
Cuts at: 0 368 2622
Size: 368 2254
EarI CTCTTCn'nnn_
Cuts at: 0 64 716 1214 1271 1502 1534 1891 2387
Size: 64 652 498 57 231 32 357 496
Cuts at: 2387 2622
Size: 235
Fragments arranged by size:
652 498 496 357 235 231 64 57
32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 450 520 859 1006 1558 1624 1693
Size: 67 383 70 339 147 552 66 69
Cuts at: 1693 2622
Size: 929
Fragments arranged by size:
929 552 383 339 147 70 69 67
66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 934 1378 2622
Size: 934 444 1244
Fragments arranged by size:
1244 934 444
EcoO109I rG'GnC_Cy
Cuts at: 0 363 1387 1620 1880 2111 2622
Size: 363 1024 233 260 231 511
Fragments arranged by size:
1024 511 363 260 233 231
EcoRI G'AATT_C
Cuts at: 0 1120 2622
Size: 1120 1502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 525 693 2622
Size: 525 168 1929
Fragments arranged by size:
1929 525 168
FspI TGC'GCA
Cuts at: 0 778 2622
Size: 778 1844
HaeII r_GCGC'y
Cuts at: 0 426 528 1753 2622
Size: 426 102 1225 869
Fragments arranged by size:
1225 869 426 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 141 173 538 570 1386 1418
Size: 16 32 93 32 365 32 816 32
Cuts at: 1418 1714 1746 2622
Size: 296 32 876
Fragments arranged by size:
876 816 365 296 93 32 32 32
32 32 16
KasI G'GCGC_C
Cuts at: 0 1749 2622
Size: 1749 873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 235 2622
Size: 71 164 2387
Fragments arranged by size:
2387 164 71
MscI TGG'CCA
Cuts at: 0 706 1030 1354 1744 2622
Size: 706 324 324 390 878
Fragments arranged by size:
878 706 390 324 324
MslI CAynn'nnrTG
Cuts at: 0 1314 2125 2155 2435 2521 2622
Size: 1314 811 30 280 86 101
Fragments arranged by size:
1314 811 280 101 86 30
MspA1I CmG'CkG
Cuts at: 0 717 961 1871 1946 2155 2174 2622
Size: 717 244 910 75 209 19 448
Fragments arranged by size:
910 717 448 244 209 75 19
NarI GG'CG_CC
Cuts at: 0 1750 2622
Size: 1750 872
NcoI C'CATG_G
Cuts at: 0 1754 2622
Size: 1754 868
PflMI CCAn_nnn'nTGG
Cuts at: 0 2019 2622
Size: 2019 603
PfoI T'CCnGG_A
Cuts at: 0 83 1601 1838 2622
Size: 83 1518 237 784
Fragments arranged by size:
1518 784 237 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2347 2379 2622
Size: 2347 32 243
Fragments arranged by size:
2347 243 32
PpuMI rG'GwC_Cy
Cuts at: 0 2111 2622
Size: 2111 511
PspOMI G'GGCC_C
Cuts at: 0 363 1387 1879 2622
Size: 363 1024 492 743
Fragments arranged by size:
1024 743 492 363
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1761 1793 2622
Size: 1761 32 829
Fragments arranged by size:
1761 829 32
PstI C_TGCA'G
Cuts at: 0 413 841 889 1688 2622
Size: 413 428 48 799 934
Fragments arranged by size:
934 799 428 413 48
PvuII CAG'CTG
Cuts at: 0 717 961 2155 2174 2622
Size: 717 244 1194 19 448
Fragments arranged by size:
1194 717 448 244 19
SacII CC_GC'GG
Cuts at: 0 1872 2622
Size: 1872 750
SexAI A'CCwGG_T
Cuts at: 0 992 2622
Size: 992 1630
SfcI C'TryA_G
Cuts at: 0 409 837 885 1684 2276 2622
Size: 409 428 48 799 592 346
Fragments arranged by size:
799 592 428 409 346 48
SfoI GGC'GCC
Cuts at: 0 1751 2622
Size: 1751 871
SmaI CCC'GGG
Cuts at: 0 2109 2622
Size: 2109 513
SmlI C'TyrA_G
Cuts at: 0 64 667 1045 1726 2622
Size: 64 603 378 681 896
Fragments arranged by size:
896 681 603 378 64
SspI AAT'ATT
Cuts at: 0 2409 2622
Size: 2409 213
StuI AGG'CCT
Cuts at: 0 205 2056 2622
Size: 205 1851 566
Fragments arranged by size:
1851 566 205
StyI C'CwwG_G
Cuts at: 0 896 1754 2018 2622
Size: 896 858 264 604
Fragments arranged by size:
896 858 604 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 519 909 1592 2488 2622
Size: 214 305 390 683 896 134
Fragments arranged by size:
896 683 390 305 214 134
TatI w'GTAC_w
Cuts at: 0 1412 1691 2622
Size: 1412 279 931
Fragments arranged by size:
1412 931 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 2622
Size: 578 32 2012
Fragments arranged by size:
2012 578 32
Tth111I GACn'n_nGTC
Cuts at: 0 517 1656 2622
Size: 517 1139 966
Fragments arranged by size:
1139 966 517
XbaI T'CTAG_A
Cuts at: 0 1698 2622
Size: 1698 924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 714 1668 1752 2622
Size: 714 954 84 870
Fragments arranged by size:
954 870 714 84
XmaI C'CCGG_G
Cuts at: 0 2107 2622
Size: 2107 515
XmnI GAAnn'nnTTC
Cuts at: 0 10 2622
Size: 10 2612
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BssHII BssSI BstBI BstXI BstYI
BtgI BtsI DrdI EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NarI NcoI PflMI PfoI PpiI
PpuMI PspOMI PsrI PstI PvuII SacII SexAI SfcI
SfoI SmaI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BlpI BmtI BplI
BsaI BsaBI BsaWI BsiWI BspEI BsrBI BsrFI BsrGI
BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EciI EcoICRI EcoRV FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI XhoI ZraI