Restriction map for
pF1KB4718
(Linear) (Six-base) MAPSORT of: KIBB4718.seq Check: 414 from: 1 to: 633
KIBB4718.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 0 182 633
Size: 182 451
Acc65I G'GTAC_C
Cuts at: 0 581 633
Size: 581 52
AflII C'TTAA_G
Cuts at: 0 277 633
Size: 277 356
AlwNI CAG_nnn'CTG
Cuts at: 0 306 633
Size: 306 327
ApaI G_GGCC'C
Cuts at: 0 167 597 633
Size: 167 430 36
Fragments arranged by size:
430 167 36
ApaLI G'TGCA_C
Cuts at: 0 69 633
Size: 69 564
AvaI C'yCGr_G
Cuts at: 0 13 633
Size: 13 620
BanI G'GyrC_C
Cuts at: 0 58 581 633
Size: 58 523 52
Fragments arranged by size:
523 58 52
BanII G_rGCy'C
Cuts at: 0 20 167 597 633
Size: 20 147 430 36
Fragments arranged by size:
430 147 36 20
BbeI G_GCGC'C
Cuts at: 0 62 633
Size: 62 571
Bme1580I G_kGCm'C
Cuts at: 0 73 167 597 633
Size: 73 94 430 36
Fragments arranged by size:
430 94 73 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 633
Size: 63 570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 304 633
Size: 60 244 329
Fragments arranged by size:
329 244 60
BsaHI Gr'CG_yC
Cuts at: 0 59 98 633
Size: 59 39 535
Fragments arranged by size:
535 59 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 633
Size: 490 30 113
Fragments arranged by size:
490 113 30
BseYI C'CCAG_C
Cuts at: 0 82 389 633
Size: 82 307 244
Fragments arranged by size:
307 244 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 633
Size: 256 377
BsiHKAI G_wGCw'C
Cuts at: 0 29 73 633
Size: 29 44 560
Fragments arranged by size:
560 44 29
Bsp1286I G_dGCh'C
Cuts at: 0 20 29 73 167 597 633
Size: 20 9 44 94 430 36
Fragments arranged by size:
430 94 44 36 20 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 464 633
Size: 314 150 169
Fragments arranged by size:
314 169 150
BsrGI T'GTAC_A
Cuts at: 0 338 633
Size: 338 295
BstEII G'GTnAC_C
Cuts at: 0 517 633
Size: 517 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 633
Size: 138 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 304 633
Size: 60 244 329
Fragments arranged by size:
329 244 60
EcoO109I rG'GnC_Cy
Cuts at: 0 163 164 594 633
Size: 163 1 430 39
Fragments arranged by size:
430 163 39 1
HaeII r_GCGC'y
Cuts at: 0 62 633
Size: 62 571
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 633
Size: 490 32 111
Fragments arranged by size:
490 111 32
HincII GTy'rAC
Cuts at: 0 291 633
Size: 291 342
HindIII A'AGCT_T
Cuts at: 0 254 633
Size: 254 379
KasI G'GCGC_C
Cuts at: 0 58 633
Size: 58 575
KpnI G_GTAC'C
Cuts at: 0 585 633
Size: 585 48
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 633
Size: 137 496
MslI CAynn'nnrTG
Cuts at: 0 223 633
Size: 223 410
MspA1I CmG'CkG
Cuts at: 0 38 578 633
Size: 38 540 55
Fragments arranged by size:
540 55 38
NarI GG'CG_CC
Cuts at: 0 59 633
Size: 59 574
NspI r_CATG'y
Cuts at: 0 77 504 633
Size: 77 427 129
Fragments arranged by size:
427 129 77
PspOMI G'GGCC_C
Cuts at: 0 163 593 633
Size: 163 430 40
Fragments arranged by size:
430 163 40
PstI C_TGCA'G
Cuts at: 0 577 633
Size: 577 56
PvuII CAG'CTG
Cuts at: 0 38 578 633
Size: 38 540 55
Fragments arranged by size:
540 55 38
SfcI C'TryA_G
Cuts at: 0 573 633
Size: 573 60
SfoI GGC'GCC
Cuts at: 0 60 633
Size: 60 573
SmlI C'TyrA_G
Cuts at: 0 277 633
Size: 277 356
StyI C'CwwG_G
Cuts at: 0 7 131 633
Size: 7 124 502
Fragments arranged by size:
502 124 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 511 633
Size: 511 122
TatI w'GTAC_w
Cuts at: 0 239 338 633
Size: 239 99 295
Fragments arranged by size:
295 239 99
Enzymes that do cut:
AccI Acc65I AflII AlwNI ApaI ApaLI AvaI BanI
BanII BbeI Bme1580I BmrI BpmI BsaHI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrGI BstEII BstXI Eco57MI
EcoO109I HaeII Hin4I HincII HindIII KasI KpnI MmeI
MslI MspA1I NarI NspI PspOMI PstI PvuII SfcI
SfoI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HpaI MfeI MluI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI