(Linear) (Six-base) MAPSORT of: KIBB4722.seq  Check: 1357  from: 1  to: 990



KIBB4722.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     716     990 
   Size:        716     274
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     835     990 
   Size:        382     453     155

  Fragments arranged by size: 

                 453     382     155
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      88     990 
   Size:         88     902
 
AlwNI CAG_nnn'CTG

Cuts at:      0     511     793     990 
   Size:        511     282     197

  Fragments arranged by size: 

                 511     282     197
 
ApoI r'AATT_y

Cuts at:      0     649     990 
   Size:        649     341
 
AvaI C'yCGr_G

Cuts at:      0     423     990 
   Size:        423     567
 
BanI G'GyrC_C

Cuts at:      0     728     990 
   Size:        728     262
 
BglII A'GATC_T

Cuts at:      0     337     990 
   Size:        337     653
 
Bme1580I G_kGCm'C

Cuts at:      0     371     990 
   Size:        371     619
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     499     990 
   Size:        499     491
 
BmtI G_CTAG'C

Cuts at:      0     169     990 
   Size:        169     821
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     990 
   Size:        463     527
 
BsaHI Gr'CG_yC

Cuts at:      0      23     990 
   Size:         23     967
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      36     990 
   Size:         36     954
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889     990 
   Size:        889     101
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      14     990 
   Size:         14     976
 
Bsp1286I G_dGCh'C

Cuts at:      0     371     990 
   Size:        371     619
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     716     990 
   Size:        716     274
 
BstYI r'GATC_y

Cuts at:      0     337     427     990 
   Size:        337      90     563

  Fragments arranged by size: 

                 563     337      90
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     918     990 
   Size:        918      72
 
BtsI GCAGTG_nn'

Cuts at:      0     134     677     990 
   Size:        134     543     313

  Fragments arranged by size: 

                 543     313     134
 
DraI TTT'AAA

Cuts at:      0     605     990 
   Size:        605     385
 
EaeI y'GGCC_r

Cuts at:      0      53     990 
   Size:         53     937
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     392     990 
   Size:        392     598
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     835     990 
   Size:        382     453     155

  Fragments arranged by size: 

                 453     382     155
 
EcoO109I rG'GnC_Cy

Cuts at:      0     536     990 
   Size:        536     454
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354     574     606     990 
   Size:        322      32     220      32     384

  Fragments arranged by size: 

                 384     322     220      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     990 
   Size:        434     556
 
MslI CAynn'nnrTG

Cuts at:      0     755     890     990 
   Size:        755     135     100

  Fragments arranged by size: 

                 755     135     100
 
NdeI CA'TA_TG

Cuts at:      0     210     990 
   Size:        210     780
 
NheI G'CTAG_C

Cuts at:      0     165     990 
   Size:        165     825
 
PpuMI rG'GwC_Cy

Cuts at:      0     536     990 
   Size:        536     454
 
PsiI TTA'TAA

Cuts at:      0     418     990 
   Size:        418     572
 
SexAI A'CCwGG_T

Cuts at:      0     830     990 
   Size:        830     160
 
SfcI C'TryA_G

Cuts at:      0     570     920     990 
   Size:        570     350      70

  Fragments arranged by size: 

                 570     350      70
 
SmlI C'TyrA_G

Cuts at:      0     478     990 
   Size:        478     512
 
SspI AAT'ATT

Cuts at:      0     766     990 
   Size:        766     224
 
StyI C'CwwG_G

Cuts at:      0       8     990 
   Size:          8     982
 
TatI w'GTAC_w

Cuts at:      0     103     990 
   Size:        103     887
 
Tth111I GACn'n_nGTC

Cuts at:      0     486     990 
   Size:        486     504
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      33     990 
   Size:         33     957
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApoI     AvaI     BanI    BglII
 Bme1580I     BmrI     BmtI    BpuEI    BsaHI    BseRI     BsgI    BsmBI
 Bsp1286I    BspMI    BstYI    BtgZI     BtsI     DraI     EaeI     EciI
  Eco57MI EcoO109I    Hin4I     MmeI     MslI     NdeI     NheI    PpuMI
     PsiI    SexAI     SfcI     SmlI     SspI     StyI     TatI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI   DraIII     DrdI     EagI     EarI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI