Restriction map for
pF1KB4740
(Linear) (Six-base) MAPSORT of: KIBB4740.seq Check: 3994 from: 1 to: 2610
KIBB4740.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 777 2331 2518 2610
Size: 777 1554 187 92
Fragments arranged by size:
1554 777 187 92
AatII G_ACGT'C
Cuts at: 0 763 2610
Size: 763 1847
Acc65I G'GTAC_C
Cuts at: 0 2398 2610
Size: 2398 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 857 1140 2080 2610
Size: 581 276 283 940 530
Fragments arranged by size:
940 581 530 283 276
AflIII A'CryG_T
Cuts at: 0 942 2610
Size: 942 1668
AgeI A'CCGG_T
Cuts at: 0 2395 2610
Size: 2395 215
AhdI GACnn_n'nnGTC
Cuts at: 0 1932 2610
Size: 1932 678
AlwNI CAG_nnn'CTG
Cuts at: 0 727 883 2610
Size: 727 156 1727
Fragments arranged by size:
1727 727 156
ApaI G_GGCC'C
Cuts at: 0 1216 2610
Size: 1216 1394
AvaI C'yCGr_G
Cuts at: 0 1025 1428 2141 2610
Size: 1025 403 713 469
Fragments arranged by size:
1025 713 469 403
BamHI G'GATC_C
Cuts at: 0 1299 1419 2372 2610
Size: 1299 120 953 238
Fragments arranged by size:
1299 953 238 120
BanI G'GyrC_C
Cuts at: 0 289 533 650 2398 2610
Size: 289 244 117 1748 212
Fragments arranged by size:
1748 289 244 212 117
BanII G_rGCy'C
Cuts at: 0 187 1216 2610
Size: 187 1029 1394
Fragments arranged by size:
1394 1029 187
BbsI GAAGACnn'nnnn_
Cuts at: 0 169 1172 2610
Size: 169 1003 1438
Fragments arranged by size:
1438 1003 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1815 1849 2610
Size: 1815 34 761
Fragments arranged by size:
1815 761 34
BglI GCCn_nnn'nGGC
Cuts at: 0 683 832 2610
Size: 683 149 1778
Fragments arranged by size:
1778 683 149
Bme1580I G_kGCm'C
Cuts at: 0 468 1216 2610
Size: 468 748 1394
Fragments arranged by size:
1394 748 468
BmgBI CAC'GTC
Cuts at: 0 943 2610
Size: 943 1667
BmrI ACTGGGnnnn_n'
Cuts at: 0 143 176 1284 2044 2610
Size: 143 33 1108 760 566
Fragments arranged by size:
1108 760 566 143 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 486 506 983 1120 1951 2023 2221
Size: 276 210 20 477 137 831 72 198
Cuts at: 2221 2254 2610
Size: 33 356
Fragments arranged by size:
831 477 356 276 210 198 137 72
33 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 801 2610
Size: 291 510 1809
Fragments arranged by size:
1809 510 291
BsaI GGTCTCn'nnnn_
Cuts at: 0 148 1905 2581 2610
Size: 148 1757 676 29
Fragments arranged by size:
1757 676 148 29
BsaAI yAC'GTr
Cuts at: 0 123 1095 1266 1542 2610
Size: 123 972 171 276 1068
Fragments arranged by size:
1068 972 276 171 123
BsaHI Gr'CG_yC
Cuts at: 0 760 2610
Size: 760 1850
BsaWI w'CCGG_w
Cuts at: 0 2395 2610
Size: 2395 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 857 887 1765 1795 2610
Size: 857 30 878 30 815
Fragments arranged by size:
878 857 815 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1666 1773 2107 2599 2610
Size: 1666 107 334 492 11
Fragments arranged by size:
1666 492 334 107 11
BseYI C'CCAG_C
Cuts at: 0 544 612 822 850 876 1004 1229 2610
Size: 544 68 210 28 26 128 225 1381
Fragments arranged by size:
1381 544 225 210 128 68 28 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 2610
Size: 758 1852
BsiEI CG_ry'CG
Cuts at: 0 1085 2610
Size: 1085 1525
BsiHKAI G_wGCw'C
Cuts at: 0 187 642 1715 2290 2610
Size: 187 455 1073 575 320
Fragments arranged by size:
1073 575 455 320 187
Bsp1286I G_dGCh'C
Cuts at: 0 187 468 642 1216 1715 2290 2610
Size: 187 281 174 574 499 575 320
Fragments arranged by size:
575 574 499 320 281 187 174
BspHI T'CATG_A
Cuts at: 0 1595 2610
Size: 1595 1015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 777 1022 1625 2331 2410 2518 2610
Size: 374 403 245 603 706 79 108 92
Fragments arranged by size:
706 603 403 374 245 108 92 79
BsrBI CCG'CTC
Cuts at: 0 987 2610
Size: 987 1623
BsrFI r'CCGG_y
Cuts at: 0 530 619 647 831 1625 2297 2395 2610
Size: 530 89 28 184 794 672 98 215
Fragments arranged by size:
794 672 530 215 184 98 89 28
BssSI C'ACGA_G
Cuts at: 0 1705 2053 2610
Size: 1705 348 557
Fragments arranged by size:
1705 557 348
BstAPI GCAn_nnn'nTGC
Cuts at: 0 554 2610
Size: 554 2056
BstBI TT'CG_AA
Cuts at: 0 1574 2610
Size: 1574 1036
BstYI r'GATC_y
Cuts at: 0 568 1020 1299 1419 2324 2372 2526 2610
Size: 568 452 279 120 905 48 154 84
Fragments arranged by size:
905 568 452 279 154 120 84 48
BtgI C'CryG_G
Cuts at: 0 1085 1838 1851 2610
Size: 1085 753 13 759
Fragments arranged by size:
1085 759 753 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1659 1818 2610
Size: 1659 159 792
Fragments arranged by size:
1659 792 159
BtsI GCAGTG_nn'
Cuts at: 0 468 1117 2610
Size: 468 649 1493
Fragments arranged by size:
1493 649 468
DraI TTT'AAA
Cuts at: 0 248 2610
Size: 248 2362
DraIII CAC_nnn'GTG
Cuts at: 0 1710 2610
Size: 1710 900
EaeI y'GGCC_r
Cuts at: 0 689 2610
Size: 689 1921
EarI CTCTTCn'nnn_
Cuts at: 0 2099 2147 2278 2362 2610
Size: 2099 48 131 84 248
Fragments arranged by size:
2099 248 131 84 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 416 2610
Size: 416 2194
EcoICRI GAG'CTC
Cuts at: 0 185 2610
Size: 185 2425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 486 506 581 857 983 1120 1140
Size: 276 210 20 75 276 126 137 20
Cuts at: 1140 1951 2023 2080 2221 2254 2610
Size: 811 72 57 141 33 356
Fragments arranged by size:
811 356 276 276 210 141 137 126
75 72 57 33 20 20
EcoO109I rG'GnC_Cy
Cuts at: 0 891 1213 1656 2610
Size: 891 322 443 954
Fragments arranged by size:
954 891 443 322
FspI TGC'GCA
Cuts at: 0 1099 2610
Size: 1099 1511
HaeII r_GCGC'y
Cuts at: 0 1846 2610
Size: 1846 764
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 780 812 1918 1950 2610
Size: 780 32 1106 32 660
Fragments arranged by size:
1106 780 660 32 32
HincII GTy'rAC
Cuts at: 0 103 303 957 2610
Size: 103 200 654 1653
Fragments arranged by size:
1653 654 200 103
KpnI G_GTAC'C
Cuts at: 0 2402 2610
Size: 2402 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 1105 1611 2610
Size: 335 770 506 999
Fragments arranged by size:
999 770 506 335
MscI TGG'CCA
Cuts at: 0 691 2610
Size: 691 1919
MslI CAynn'nnrTG
Cuts at: 0 590 1523 1600 1639 1837 2610
Size: 590 933 77 39 198 773
Fragments arranged by size:
933 773 590 198 77 39
MspA1I CmG'CkG
Cuts at: 0 354 486 880 1087 1125 2199 2556 2610
Size: 354 132 394 207 38 1074 357 54
Fragments arranged by size:
1074 394 357 354 207 132 54 38
NaeI GCC'GGC
Cuts at: 0 649 1627 2610
Size: 649 978 983
Fragments arranged by size:
983 978 649
NcoI C'CATG_G
Cuts at: 0 1838 1851 2610
Size: 1838 13 759
Fragments arranged by size:
1838 759 13
NgoMIV G'CCGG_C
Cuts at: 0 647 1625 2610
Size: 647 978 985
Fragments arranged by size:
985 978 647
NspI r_CATG'y
Cuts at: 0 351 2256 2610
Size: 351 1905 354
Fragments arranged by size:
1905 354 351
PasI CC'CwG_GG
Cuts at: 0 1209 1311 2610
Size: 1209 102 1299
Fragments arranged by size:
1299 1209 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 980 1070 1685 1967 2025 2610
Size: 980 90 615 282 58 585
Fragments arranged by size:
980 615 585 282 90 58
PfoI T'CCnGG_A
Cuts at: 0 419 571 1322 2018 2171 2610
Size: 419 152 751 696 153 439
Fragments arranged by size:
751 696 439 419 153 152
PmlI CAC'GTG
Cuts at: 0 1542 2610
Size: 1542 1068
PspOMI G'GGCC_C
Cuts at: 0 1212 2610
Size: 1212 1398
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1486 1518 2610
Size: 1486 32 1092
Fragments arranged by size:
1486 1092 32
PstI C_TGCA'G
Cuts at: 0 371 1769 2387 2610
Size: 371 1398 618 223
Fragments arranged by size:
1398 618 371 223
PvuII CAG'CTG
Cuts at: 0 354 486 2556 2610
Size: 354 132 2070 54
Fragments arranged by size:
2070 354 132 54
SacI G_AGCT'C
Cuts at: 0 187 2610
Size: 187 2423
SacII CC_GC'GG
Cuts at: 0 1088 2610
Size: 1088 1522
SapI GCTCTTCn'nnn_
Cuts at: 0 2147 2278 2610
Size: 2147 131 332
Fragments arranged by size:
2147 332 131
SfcI C'TryA_G
Cuts at: 0 367 1765 2383 2610
Size: 367 1398 618 227
Fragments arranged by size:
1398 618 367 227
SmaI CCC'GGG
Cuts at: 0 1430 2610
Size: 1430 1180
SmlI C'TyrA_G
Cuts at: 0 270 780 2610
Size: 270 510 1830
Fragments arranged by size:
1830 510 270
SphI G_CATG'C
Cuts at: 0 2256 2610
Size: 2256 354
StyI C'CwwG_G
Cuts at: 0 86 1838 1851 2610
Size: 86 1752 13 759
Fragments arranged by size:
1752 759 86 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2605 2610
Size: 2605 5
TatI w'GTAC_w
Cuts at: 0 2009 2108 2610
Size: 2009 99 502
Fragments arranged by size:
2009 502 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 857 889 2610
Size: 857 32 1721
Fragments arranged by size:
1721 857 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2169 2610
Size: 2169 441
XmaI C'CCGG_G
Cuts at: 0 1428 2610
Size: 1428 1182
ZraI GAC'GTC
Cuts at: 0 761 2610
Size: 761 1849
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AgeI AhdI AlwNI
ApaI AvaI BamHI BanI BanII BbsI BcgI BglI
Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PspOMI PsrI PstI PvuII
SacI SacII SapI SfcI SmaI SmlI SphI StyI
TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AleI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I
BsaBI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KasI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PpiI PpuMI PshAI PsiI
PspXI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmnI