(Linear) (Six-base) MAPSORT of: KIBB4753.seq  Check: 1456  from: 1  to: 324



KIBB4753.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     324 
   Size:        229      95
 
AflIII A'CryG_T

Cuts at:      0      77     324 
   Size:         77     247
 
ApaLI G'TGCA_C

Cuts at:      0      91     324 
   Size:         91     233
 
Bme1580I G_kGCm'C

Cuts at:      0      85      95     324 
   Size:         85      10     229

  Fragments arranged by size: 

                 229      85      10
 
BsaAI yAC'GTr

Cuts at:      0      80      90     324 
   Size:         80      10     234

  Fragments arranged by size: 

                 234      80      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     324 
   Size:         58     266
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     324 
   Size:         95     229
 
Bsp1286I G_dGCh'C

Cuts at:      0      85      95     324 
   Size:         85      10     229

  Fragments arranged by size: 

                 229      85      10
 
BsrBI CCG'CTC

Cuts at:      0     254     324 
   Size:        254      70
 
BsrDI GCAATG_nn'

Cuts at:      0     300     324 
   Size:        300      24
 
BssSI C'ACGA_G

Cuts at:      0     224     324 
   Size:        224     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      80     324 
   Size:         80     244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     324 
   Size:        229      95
 
HincII GTy'rAC

Cuts at:      0     186     324 
   Size:        186     138
 
MslI CAynn'nnrTG

Cuts at:      0     224     324 
   Size:        224     100
 
MspA1I CmG'CkG

Cuts at:      0     169     314     324 
   Size:        169     145      10

  Fragments arranged by size: 

                 169     145      10
 
PmlI CAC'GTG

Cuts at:      0      80     324 
   Size:         80     244
 
PstI C_TGCA'G

Cuts at:      0     198     261     324 
   Size:        198      63      63

  Fragments arranged by size: 

                 198      63      63
 
PvuII CAG'CTG

Cuts at:      0     169     324 
   Size:        169     155
 
SfcI C'TryA_G

Cuts at:      0     194     257     324 
   Size:        194      63      67

  Fragments arranged by size: 

                 194      67      63
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI Bme1580I    BsaAI    BseRI  BsiHKAI Bsp1286I
    BsrBI    BsrDI    BssSI   BstAPI  Eco57MI   HincII     MslI   MspA1I
     PmlI     PstI    PvuII     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrFI
    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI