Restriction map for
pF1KB4757
(Linear) (Six-base) MAPSORT of: KIBB4757.seq Check: 5482 from: 1 to: 1632
KIBB4757.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1351 1632
Size: 1351 281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1566 1632
Size: 41 1525 66
Fragments arranged by size:
1525 66 41
AflIII A'CryG_T
Cuts at: 0 346 550 1632
Size: 346 204 1082
Fragments arranged by size:
1082 346 204
AleI CACnn'nnGTG
Cuts at: 0 619 869 1457 1517 1541 1625 1632
Size: 619 250 588 60 24 84 7
Fragments arranged by size:
619 588 250 84 60 24 7
ApoI r'AATT_y
Cuts at: 0 608 692 735 776 860 944 1028 1112
Size: 608 84 43 41 84 84 84 84
Cuts at: 1112 1364 1532 1632
Size: 252 168 100
Fragments arranged by size:
608 252 168 100 84 84 84 84
84 43 41
AvrII C'CTAG_G
Cuts at: 0 205 1632
Size: 205 1427
BanI G'GyrC_C
Cuts at: 0 1271 1632
Size: 1271 361
BanII G_rGCy'C
Cuts at: 0 502 1090 1632
Size: 502 588 542
Fragments arranged by size:
588 542 502
BfrBI ATG'CAT
Cuts at: 0 178 1632
Size: 178 1454
BmgBI CAC'GTC
Cuts at: 0 349 1632
Size: 349 1283
BmrI ACTGGGnnnn_n'
Cuts at: 0 962 1632
Size: 962 670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1109 1632
Size: 1109 523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 554 638 722 806 1142 1310 1394
Size: 103 451 84 84 84 336 168 84
Cuts at: 1394 1632
Size: 238
Fragments arranged by size:
451 336 238 168 103 84 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1632
Size: 318 1314
BsaI GGTCTCn'nnnn_
Cuts at: 0 534 1206 1632
Size: 534 672 426
Fragments arranged by size:
672 534 426
BsaBI GATnn'nnATC
Cuts at: 0 666 1632
Size: 666 966
BseYI C'CCAG_C
Cuts at: 0 56 1632
Size: 56 1576
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 1632
Size: 429 1203
BsiHKAI G_wGCw'C
Cuts at: 0 502 1174 1632
Size: 502 672 458
Fragments arranged by size:
672 502 458
BsmI GAATG_Cn'
Cuts at: 0 823 1632
Size: 823 809
BsmBI CGTCTCn'nnnn_
Cuts at: 0 355 1632
Size: 355 1277
Bsp1286I G_dGCh'C
Cuts at: 0 502 1090 1174 1632
Size: 502 588 84 458
Fragments arranged by size:
588 502 458 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1351 1632
Size: 1351 281
BsrDI GCAATG_nn'
Cuts at: 0 729 1317 1632
Size: 729 588 315
Fragments arranged by size:
729 588 315
BstBI TT'CG_AA
Cuts at: 0 912 1632
Size: 912 720
BtsI GCAGTG_nn'
Cuts at: 0 649 897 1233 1632
Size: 649 248 336 399
Fragments arranged by size:
649 399 336 248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 711 746 795 830 1299 1334 1632
Size: 711 35 49 35 469 35 298
Fragments arranged by size:
711 469 298 49 35 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 17 33 362 909 1632
Size: 17 16 329 547 723
Fragments arranged by size:
723 547 329 17 16
EcoICRI GAG'CTC
Cuts at: 0 500 1632
Size: 500 1132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 103 554 638 722 806 1142 1310
Size: 41 62 451 84 84 84 336 168
Cuts at: 1310 1394 1566 1632
Size: 84 172 66
Fragments arranged by size:
451 336 172 168 84 84 84 84
66 62 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 1632
Size: 232 1400
EcoRI G'AATT_C
Cuts at: 0 608 692 735 776 860 944 1028 1112
Size: 608 84 43 41 84 84 84 84
Cuts at: 1112 1364 1532 1632
Size: 252 168 100
Fragments arranged by size:
608 252 168 100 84 84 84 84
84 43 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 62 1632
Size: 62 1570
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 363 395 1632
Size: 363 32 1237
Fragments arranged by size:
1237 363 32
HindIII A'AGCT_T
Cuts at: 0 318 1632
Size: 318 1314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1632
Size: 460 1172
MslI CAynn'nnrTG
Cuts at: 0 619 633 660 869 971 1137 1457 1517
Size: 619 14 27 209 102 166 320 60
Cuts at: 1517 1541 1625 1632
Size: 24 84 7
Fragments arranged by size:
619 320 209 166 102 84 60 27
24 14 7
NdeI CA'TA_TG
Cuts at: 0 630 886 1358 1632
Size: 630 256 472 274
Fragments arranged by size:
630 472 274 256
NsiI A_TGCA'T
Cuts at: 0 180 1632
Size: 180 1452
NspI r_CATG'y
Cuts at: 0 182 554 1632
Size: 182 372 1078
Fragments arranged by size:
1078 372 182
PciI A'CATG_T
Cuts at: 0 550 1632
Size: 550 1082
PfoI T'CCnGG_A
Cuts at: 0 233 1632
Size: 233 1399
PsiI TTA'TAA
Cuts at: 0 716 800 1632
Size: 716 84 832
Fragments arranged by size:
832 716 84
SacI G_AGCT'C
Cuts at: 0 502 1632
Size: 502 1130
SapI GCTCTTCn'nnn_
Cuts at: 0 909 1632
Size: 909 723
SfcI C'TryA_G
Cuts at: 0 362 1632
Size: 362 1270
SmlI C'TyrA_G
Cuts at: 0 333 1632
Size: 333 1299
StyI C'CwwG_G
Cuts at: 0 205 1632
Size: 205 1427
XmnI GAAnn'nnTTC
Cuts at: 0 739 823 991 1495 1632
Size: 739 84 168 504 137
Fragments arranged by size:
739 504 168 137 84
Enzymes that do cut:
AarI AcuI AflIII AleI ApoI AvrII BanI BanII
BfrBI BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BstBI BtsI CspCI EarI EcoICRI Eco57MI EcoNI EcoRI
FalI Hin4I HindIII MmeI MslI NdeI NsiI NspI
PciI PfoI PsiI SacI SapI SfcI SmlI StyI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmtI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII
DrdI EaeI EagI EciI EcoO109I EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI