Restriction map for
pF1KB4774
(Linear) (Six-base) MAPSORT of: KIBB4774.seq Check: 4087 from: 1 to: 2190
KIBB4774.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 370 2190
Size: 370 1820
AccI GT'mk_AC
Cuts at: 0 1289 2190
Size: 1289 901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 2190
Size: 769 1421
AleI CACnn'nnGTG
Cuts at: 0 485 2190
Size: 485 1705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1906 2190
Size: 1906 284
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 814 846 1078 1110 2190
Size: 814 32 232 32 1080
Fragments arranged by size:
1080 814 232 32 32
ApaI G_GGCC'C
Cuts at: 0 195 586 2190
Size: 195 391 1604
Fragments arranged by size:
1604 391 195
ApoI r'AATT_y
Cuts at: 0 1243 2155 2190
Size: 1243 912 35
Fragments arranged by size:
1243 912 35
AvaI C'yCGr_G
Cuts at: 0 31 1066 1726 1812 2011 2132 2190
Size: 31 1035 660 86 199 121 58
Fragments arranged by size:
1035 660 199 121 86 58 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 842 875 2190
Size: 842 33 1315
Fragments arranged by size:
1315 842 33
BamHI G'GATC_C
Cuts at: 0 1391 2190
Size: 1391 799
BanI G'GyrC_C
Cuts at: 0 1167 1685 2190
Size: 1167 518 505
Fragments arranged by size:
1167 518 505
BanII G_rGCy'C
Cuts at: 0 119 195 586 2190
Size: 119 76 391 1604
Fragments arranged by size:
1604 391 119 76
BbeI G_GCGC'C
Cuts at: 0 1171 2190
Size: 1171 1019
BbsI GAAGACnn'nnnn_
Cuts at: 0 441 2190
Size: 441 1749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 251 799 833 1444 1478 1497 1531
Size: 217 34 548 34 611 34 19 34
Cuts at: 1531 2113 2147 2190
Size: 582 34 43
Fragments arranged by size:
611 582 548 217 43 34 34 34
34 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 843 2190
Size: 843 1347
BfrBI ATG'CAT
Cuts at: 0 810 2190
Size: 810 1380
BlpI GC'TnA_GC
Cuts at: 0 913 2190
Size: 913 1277
Bme1580I G_kGCm'C
Cuts at: 0 195 586 958 1496 2190
Size: 195 391 372 538 694
Fragments arranged by size:
694 538 391 372 195
BmgBI CAC'GTC
Cuts at: 0 651 1094 1562 2190
Size: 651 443 468 628
Fragments arranged by size:
651 628 468 443
BmrI ACTGGGnnnn_n'
Cuts at: 0 480 1869 2022 2190
Size: 480 1389 153 168
Fragments arranged by size:
1389 480 168 153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 2135 2190
Size: 126 2009 55
Fragments arranged by size:
2009 126 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 520 816 1179 1498 2190
Size: 181 339 296 363 319 692
Fragments arranged by size:
692 363 339 319 296 181
Bpu10I CC'TnA_GC
Cuts at: 0 1205 2190
Size: 1205 985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 2190
Size: 1564 626
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 964 2190
Size: 778 186 1226
Fragments arranged by size:
1226 778 186
BsaAI yAC'GTr
Cuts at: 0 564 1416 2190
Size: 564 852 774
Fragments arranged by size:
852 774 564
BsaHI Gr'CG_yC
Cuts at: 0 367 1168 2139 2190
Size: 367 801 971 51
Fragments arranged by size:
971 801 367 51
BsaWI w'CCGG_w
Cuts at: 0 2177 2190
Size: 2177 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 757 787 814 844 985 1015 1870 1900
Size: 757 30 27 30 141 30 855 30
Cuts at: 1900 2041 2071 2190
Size: 141 30 119
Fragments arranged by size:
855 757 141 141 119 30 30 30
30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 990 2190
Size: 115 875 1200
Fragments arranged by size:
1200 875 115
BseYI C'CCAG_C
Cuts at: 0 283 642 957 1369 2190
Size: 283 359 315 412 821
Fragments arranged by size:
821 412 359 315 283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1734 2185 2190
Size: 1734 451 5
Fragments arranged by size:
1734 451 5
BsiEI CG_ry'CG
Cuts at: 0 60 64 2190
Size: 60 4 2126
Fragments arranged by size:
2126 60 4
BsiHKAI G_wGCw'C
Cuts at: 0 298 1561 1654 2190
Size: 298 1263 93 536
Fragments arranged by size:
1263 536 298 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1221 1593 2190
Size: 1221 372 597
Fragments arranged by size:
1221 597 372
Bsp1286I G_dGCh'C
Cuts at: 0 119 195 298 586 958 1496 1561 1654
Size: 119 76 103 288 372 538 65 93
Cuts at: 1654 2190
Size: 536
Fragments arranged by size:
538 536 372 288 119 103 93 76
65
BsrDI GCAATG_nn'
Cuts at: 0 164 926 1926 2190
Size: 164 762 1000 264
Fragments arranged by size:
1000 762 264 164
BsrFI r'CCGG_y
Cuts at: 0 59 353 1364 2190
Size: 59 294 1011 826
Fragments arranged by size:
1011 826 294 59
BssSI C'ACGA_G
Cuts at: 0 637 2190
Size: 637 1553
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1379 2190
Size: 1379 811
BstYI r'GATC_y
Cuts at: 0 216 343 1391 2190
Size: 216 127 1048 799
Fragments arranged by size:
1048 799 216 127
Bsu36I CC'TnA_GG
Cuts at: 0 187 761 2190
Size: 187 574 1429
Fragments arranged by size:
1429 574 187
BtgI C'CryG_G
Cuts at: 0 573 1665 2190
Size: 573 1092 525
Fragments arranged by size:
1092 573 525
BtsI GCAGTG_nn'
Cuts at: 0 1038 1962 2190
Size: 1038 924 228
Fragments arranged by size:
1038 924 228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1977 2012 2190
Size: 1977 35 178
Fragments arranged by size:
1977 178 35
DraIII CAC_nnn'GTG
Cuts at: 0 1044 2190
Size: 1044 1146
EaeI y'GGCC_r
Cuts at: 0 57 61 74 679 897 1329 2190
Size: 57 4 13 605 218 432 861
Fragments arranged by size:
861 605 432 218 57 13 4
EagI C'GGCC_G
Cuts at: 0 57 61 2190
Size: 57 4 2129
Fragments arranged by size:
2129 57 4
EarI CTCTTCn'nnn_
Cuts at: 0 173 2190
Size: 173 2017
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 1404 1790 2190
Size: 112 1292 386 400
Fragments arranged by size:
1292 400 386 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 520 769 816 1179 1498 2190
Size: 181 339 249 47 363 319 692
Fragments arranged by size:
692 363 339 319 249 181 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 2190
Size: 97 2093
EcoO109I rG'GnC_Cy
Cuts at: 0 183 191 192 582 583 2190
Size: 183 8 1 390 1 1607
Fragments arranged by size:
1607 390 183 8 1 1
EcoRI G'AATT_C
Cuts at: 0 1243 2190
Size: 1243 947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1296 1956 2112 2190
Size: 1296 660 156 78
Fragments arranged by size:
1296 660 156 78
FseI GG_CCGG'CC
Cuts at: 0 63 2190
Size: 63 2127
FspI TGC'GCA
Cuts at: 0 1954 2190
Size: 1954 236
HaeII r_GCGC'y
Cuts at: 0 1171 1452 1524 1755 2190
Size: 1171 281 72 231 435
Fragments arranged by size:
1171 435 281 231 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 744 757 776 789 1636 1668 2041 2073
Size: 744 13 19 13 847 32 373 32
Cuts at: 2073 2190
Size: 117
Fragments arranged by size:
847 744 373 117 32 32 19 13
13
HincII GTy'rAC
Cuts at: 0 1626 2190
Size: 1626 564
HindIII A'AGCT_T
Cuts at: 0 943 2190
Size: 943 1247
KasI G'GCGC_C
Cuts at: 0 1167 2190
Size: 1167 1023
MfeI C'AATT_G
Cuts at: 0 1260 2190
Size: 1260 930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 2084 2190
Size: 1248 836 106
Fragments arranged by size:
1248 836 106
MscI TGG'CCA
Cuts at: 0 899 1331 2190
Size: 899 432 859
Fragments arranged by size:
899 859 432
MslI CAynn'nnrTG
Cuts at: 0 485 2044 2190
Size: 485 1559 146
Fragments arranged by size:
1559 485 146
MspA1I CmG'CkG
Cuts at: 0 24 66 287 575 1460 1667 1683 1718
Size: 24 42 221 288 885 207 16 35
Cuts at: 1718 1757 1860 1969 2190
Size: 39 103 109 221
Fragments arranged by size:
885 288 221 221 207 109 103 42
39 35 24 16
NaeI GCC'GGC
Cuts at: 0 61 355 2190
Size: 61 294 1835
Fragments arranged by size:
1835 294 61
NarI GG'CG_CC
Cuts at: 0 1168 2190
Size: 1168 1022
NgoMIV G'CCGG_C
Cuts at: 0 59 353 2190
Size: 59 294 1837
Fragments arranged by size:
1837 294 59
NsiI A_TGCA'T
Cuts at: 0 812 2190
Size: 812 1378
NspI r_CATG'y
Cuts at: 0 1038 2043 2190
Size: 1038 1005 147
Fragments arranged by size:
1038 1005 147
PasI CC'CwG_GG
Cuts at: 0 777 1698 2190
Size: 777 921 492
Fragments arranged by size:
921 777 492
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 964 2190
Size: 642 322 1226
Fragments arranged by size:
1226 642 322
PfoI T'CCnGG_A
Cuts at: 0 264 497 845 2190
Size: 264 233 348 1345
Fragments arranged by size:
1345 348 264 233
PmlI CAC'GTG
Cuts at: 0 1416 2190
Size: 1416 774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 2190
Size: 814 32 1344
Fragments arranged by size:
1344 814 32
PpuMI rG'GwC_Cy
Cuts at: 0 183 2190
Size: 183 2007
PspOMI G'GGCC_C
Cuts at: 0 191 582 2190
Size: 191 391 1608
Fragments arranged by size:
1608 391 191
PstI C_TGCA'G
Cuts at: 0 261 1895 2126 2190
Size: 261 1634 231 64
Fragments arranged by size:
1634 261 231 64
PvuII CAG'CTG
Cuts at: 0 287 1460 1718 1860 1969 2190
Size: 287 1173 258 142 109 221
Fragments arranged by size:
1173 287 258 221 142 109
SacII CC_GC'GG
Cuts at: 0 576 1668 2190
Size: 576 1092 522
Fragments arranged by size:
1092 576 522
SfcI C'TryA_G
Cuts at: 0 257 1891 2122 2190
Size: 257 1634 231 68
Fragments arranged by size:
1634 257 231 68
SfoI GGC'GCC
Cuts at: 0 1169 2190
Size: 1169 1021
SmaI CCC'GGG
Cuts at: 0 1068 1728 1814 2190
Size: 1068 660 86 376
Fragments arranged by size:
1068 660 376 86
SmlI C'TyrA_G
Cuts at: 0 1579 2190
Size: 1579 611
SrfI GCCC'GGGC
Cuts at: 0 1814 2190
Size: 1814 376
StyI C'CwwG_G
Cuts at: 0 1823 2190
Size: 1823 367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1888 2190
Size: 1888 302
TatI w'GTAC_w
Cuts at: 0 2030 2190
Size: 2030 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 383 2190
Size: 383 1807
XmaI C'CCGG_G
Cuts at: 0 1066 1726 1812 2190
Size: 1066 660 86 378
Fragments arranged by size:
1066 660 378 86
XmnI GAAnn'nnTTC
Cuts at: 0 273 826 2190
Size: 273 553 1364
Fragments arranged by size:
1364 553 273
ZraI GAC'GTC
Cuts at: 0 368 2190
Size: 368 1822
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AloI ApaI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI
BssSI BstAPI BstYI Bsu36I BtgI BtsI CspCI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII SacII SfcI SfoI SmaI SmlI
SrfI StyI TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlwNI ApaLI AscI AseI AsiSI AvrII BbvCI BclI
BglI BglII BmtI BsaBI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I
BtgZI ClaI DraI DrdI EcoICRI EcoRV FspAI HpaI
KpnI MluI NcoI NdeI NheI NotI NruI PacI
PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
TstI Tth111I XbaI XhoI