Restriction map for
pF1KB4782
(Linear) (Six-base) MAPSORT of: KIBB4782.seq Check: 1999 from: 1 to: 1041
KIBB4782.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 298 1041
Size: 298 743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 675 1041
Size: 340 335 366
Fragments arranged by size:
366 340 335
AflIII A'CryG_T
Cuts at: 0 632 740 1041
Size: 632 108 301
Fragments arranged by size:
632 301 108
AhdI GACnn_n'nnGTC
Cuts at: 0 792 1041
Size: 792 249
AlwNI CAG_nnn'CTG
Cuts at: 0 57 1041
Size: 57 984
ApaLI G'TGCA_C
Cuts at: 0 997 1041
Size: 997 44
AvaI C'yCGr_G
Cuts at: 0 68 910 1041
Size: 68 842 131
Fragments arranged by size:
842 131 68
BamHI G'GATC_C
Cuts at: 0 755 1041
Size: 755 286
BanII G_rGCy'C
Cuts at: 0 838 1041
Size: 838 203
BbsI GAAGACnn'nnnn_
Cuts at: 0 313 449 1041
Size: 313 136 592
Fragments arranged by size:
592 313 136
BciVI GTATCCnnnnn_n'
Cuts at: 0 985 1041
Size: 985 56
BclI T'GATC_A
Cuts at: 0 500 1041
Size: 500 541
BfrBI ATG'CAT
Cuts at: 0 591 1041
Size: 591 450
BglII A'GATC_T
Cuts at: 0 332 1041
Size: 332 709
Bme1580I G_kGCm'C
Cuts at: 0 81 1001 1041
Size: 81 920 40
Fragments arranged by size:
920 81 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 939 1041
Size: 939 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 814 1041
Size: 487 327 227
Fragments arranged by size:
487 327 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 694 972 1041
Size: 694 278 69
Fragments arranged by size:
694 278 69
BsaAI yAC'GTr
Cuts at: 0 996 1041
Size: 996 45
BsaBI GATnn'nnATC
Cuts at: 0 1021 1041
Size: 1021 20
BsaHI Gr'CG_yC
Cuts at: 0 350 653 730 1041
Size: 350 303 77 311
Fragments arranged by size:
350 311 303 77
BsaWI w'CCGG_w
Cuts at: 0 747 1041
Size: 747 294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1041
Size: 61 980
BseYI C'CCAG_C
Cuts at: 0 20 1041
Size: 20 1021
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 583 591 1041
Size: 349 234 8 450
Fragments arranged by size:
450 349 234 8
BsiHKAI G_wGCw'C
Cuts at: 0 587 920 1001 1041
Size: 587 333 81 40
Fragments arranged by size:
587 333 81 40
BsmI GAATG_Cn'
Cuts at: 0 591 1041
Size: 591 450
Bsp1286I G_dGCh'C
Cuts at: 0 81 587 838 920 1001 1041
Size: 81 506 251 82 81 40
Fragments arranged by size:
506 251 82 81 81 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 298 1041
Size: 298 743
BsrGI T'GTAC_A
Cuts at: 0 278 1041
Size: 278 763
BssSI C'ACGA_G
Cuts at: 0 82 1041
Size: 82 959
BstAPI GCAn_nnn'nTGC
Cuts at: 0 57 1041
Size: 57 984
BstEII G'GTnAC_C
Cuts at: 0 667 1041
Size: 667 374
BstYI r'GATC_y
Cuts at: 0 332 755 1041
Size: 332 423 286
Fragments arranged by size:
423 332 286
BtgI C'CryG_G
Cuts at: 0 129 578 647 1041
Size: 129 449 69 394
Fragments arranged by size:
449 394 129 69
BtsI GCAGTG_nn'
Cuts at: 0 895 1041
Size: 895 146
DraIII CAC_nnn'GTG
Cuts at: 0 1005 1041
Size: 1005 36
EaeI y'GGCC_r
Cuts at: 0 117 1007 1041
Size: 117 890 34
Fragments arranged by size:
890 117 34
EarI CTCTTCn'nnn_
Cuts at: 0 868 1041
Size: 868 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 1041
Size: 244 797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 487 675 814 1041
Size: 340 147 188 139 227
Fragments arranged by size:
340 227 188 147 139
EcoO109I rG'GnC_Cy
Cuts at: 0 166 486 1041
Size: 166 320 555
Fragments arranged by size:
555 320 166
HaeII r_GCGC'y
Cuts at: 0 578 1041
Size: 578 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 300 332 778 810 929 961 971 1003
Size: 300 32 446 32 119 32 10 32
Cuts at: 1003 1041
Size: 38
Fragments arranged by size:
446 300 119 38 32 32 32 32
10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 809 1041
Size: 167 642 232
Fragments arranged by size:
642 232 167
MscI TGG'CCA
Cuts at: 0 1009 1041
Size: 1009 32
MslI CAynn'nnrTG
Cuts at: 0 1027 1041
Size: 1027 14
MspA1I CmG'CkG
Cuts at: 0 567 1041
Size: 567 474
NcoI C'CATG_G
Cuts at: 0 129 1041
Size: 129 912
NsiI A_TGCA'T
Cuts at: 0 593 1041
Size: 593 448
NspI r_CATG'y
Cuts at: 0 98 126 591 744 954 1041
Size: 98 28 465 153 210 87
Fragments arranged by size:
465 210 153 98 87 28
PasI CC'CwG_GG
Cuts at: 0 162 1041
Size: 162 879
PciI A'CATG_T
Cuts at: 0 740 1041
Size: 740 301
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 1041
Size: 162 879
PfoI T'CCnGG_A
Cuts at: 0 533 1041
Size: 533 508
PpuMI rG'GwC_Cy
Cuts at: 0 486 1041
Size: 486 555
PshAI GACnn'nnGTC
Cuts at: 0 674 1041
Size: 674 367
PspXI vC'TCGA_Gb
Cuts at: 0 68 1041
Size: 68 973
PvuII CAG'CTG
Cuts at: 0 567 1041
Size: 567 474
ScaI AGT'ACT
Cuts at: 0 508 556 1041
Size: 508 48 485
Fragments arranged by size:
508 485 48
SexAI A'CCwGG_T
Cuts at: 0 1001 1041
Size: 1001 40
SmaI CCC'GGG
Cuts at: 0 912 1041
Size: 912 129
SmlI C'TyrA_G
Cuts at: 0 68 1041
Size: 68 973
SphI G_CATG'C
Cuts at: 0 98 1041
Size: 98 943
StuI AGG'CCT
Cuts at: 0 66 1041
Size: 66 975
StyI C'CwwG_G
Cuts at: 0 61 129 353 672 797 1041
Size: 61 68 224 319 125 244
Fragments arranged by size:
319 244 224 125 68 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 422 1041
Size: 422 619
TatI w'GTAC_w
Cuts at: 0 278 506 554 1041
Size: 278 228 48 487
Fragments arranged by size:
487 278 228 48
Tth111I GACn'n_nGTC
Cuts at: 0 370 1041
Size: 370 671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 127 294 1041
Size: 127 167 747
Fragments arranged by size:
747 167 127
XhoI C'TCGA_G
Cuts at: 0 68 1041
Size: 68 973
XmaI C'CCGG_G
Cuts at: 0 910 1041
Size: 910 131
XmnI GAAnn'nnTTC
Cuts at: 0 862 1041
Size: 862 179
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApaLI AvaI BamHI
BanII BbsI BciVI BclI BfrBI BglII Bme1580I BplI
BpmI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI BssSI BstAPI
BstEII BstYI BtgI BtsI DraIII EaeI EarI EciI
Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I
NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI
PshAI PspXI PvuII ScaI SexAI SmaI SmlI SphI
StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BglI BlpI BmgBI
BmrI BmtI Bpu10I BpuEI BsaXI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PsiI PspOMI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TstI XbaI ZraI