Restriction map for
pF1KB4783
(Linear) (Six-base) MAPSORT of: KIBB4783.seq Check: 179 from: 1 to: 1956
KIBB4783.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 537 1956
Size: 537 1419
AatII G_ACGT'C
Cuts at: 0 675 1956
Size: 675 1281
Acc65I G'GTAC_C
Cuts at: 0 1309 1956
Size: 1309 647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1466 1956
Size: 1466 490
AleI CACnn'nnGTG
Cuts at: 0 822 1956
Size: 822 1134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 761 793 1956
Size: 761 32 1163
Fragments arranged by size:
1163 761 32
AlwNI CAG_nnn'CTG
Cuts at: 0 819 1842 1956
Size: 819 1023 114
Fragments arranged by size:
1023 819 114
ApaLI G'TGCA_C
Cuts at: 0 1270 1956
Size: 1270 686
ApoI r'AATT_y
Cuts at: 0 88 159 1635 1956
Size: 88 71 1476 321
Fragments arranged by size:
1476 321 88 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1293 1326 1956
Size: 1293 33 630
Fragments arranged by size:
1293 630 33
BanI G'GyrC_C
Cuts at: 0 1167 1309 1681 1705 1956
Size: 1167 142 372 24 251
Fragments arranged by size:
1167 372 251 142 24
BanII G_rGCy'C
Cuts at: 0 143 1792 1956
Size: 143 1649 164
Fragments arranged by size:
1649 164 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 430 1956
Size: 430 1526
BciVI GTATCCnnnnn_n'
Cuts at: 0 1114 1956
Size: 1114 842
BclI T'GATC_A
Cuts at: 0 1232 1956
Size: 1232 724
BglI GCCn_nnn'nGGC
Cuts at: 0 810 1387 1956
Size: 810 577 569
Fragments arranged by size:
810 577 569
BglII A'GATC_T
Cuts at: 0 650 1956
Size: 650 1306
Bme1580I G_kGCm'C
Cuts at: 0 1274 1956
Size: 1274 682
BmrI ACTGGGnnnn_n'
Cuts at: 0 1781 1956
Size: 1781 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 919 928 1956
Size: 709 210 9 1028
Fragments arranged by size:
1028 709 210 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 1956
Size: 1709 247
BsaAI yAC'GTr
Cuts at: 0 1951 1956
Size: 1951 5
BsaHI Gr'CG_yC
Cuts at: 0 672 1956
Size: 672 1284
BsaWI w'CCGG_w
Cuts at: 0 177 1469 1956
Size: 177 1292 487
Fragments arranged by size:
1292 487 177
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 1442 1550 1913 1916 1919 1928 1931
Size: 23 1419 108 363 3 3 9 3
Cuts at: 1931 1956
Size: 25
Fragments arranged by size:
1419 363 108 25 23 9 3 3
3
BseYI C'CCAG_C
Cuts at: 0 533 1956
Size: 533 1423
BsiHKAI G_wGCw'C
Cuts at: 0 143 609 1274 1653 1792 1956
Size: 143 466 665 379 139 164
Fragments arranged by size:
665 466 379 164 143 139
BsmI GAATG_Cn'
Cuts at: 0 755 1956
Size: 755 1201
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1041 1956
Size: 1041 915
Bsp1286I G_dGCh'C
Cuts at: 0 143 609 1274 1653 1792 1956
Size: 143 466 665 379 139 164
Fragments arranged by size:
665 466 379 164 143 139
BspEI T'CCGG_A
Cuts at: 0 177 1956
Size: 177 1779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 537 1956
Size: 537 1419
BsrDI GCAATG_nn'
Cuts at: 0 401 1956
Size: 401 1555
BsrFI r'CCGG_y
Cuts at: 0 1817 1956
Size: 1817 139
BstYI r'GATC_y
Cuts at: 0 650 776 1956
Size: 650 126 1180
Fragments arranged by size:
1180 650 126
BtgI C'CryG_G
Cuts at: 0 42 1107 1200 1956
Size: 42 1065 93 756
Fragments arranged by size:
1065 756 93 42
BtsI GCAGTG_nn'
Cuts at: 0 1623 1956
Size: 1623 333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1530 1565 1956
Size: 1530 35 391
Fragments arranged by size:
1530 391 35
EaeI y'GGCC_r
Cuts at: 0 384 551 838 1090 1281 1551 1956
Size: 384 167 287 252 191 270 405
Fragments arranged by size:
405 384 287 270 252 191 167
EarI CTCTTCn'nnn_
Cuts at: 0 577 1370 1956
Size: 577 793 586
Fragments arranged by size:
793 586 577
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1451 1956
Size: 1451 505
EcoICRI GAG'CTC
Cuts at: 0 141 1790 1956
Size: 141 1649 166
Fragments arranged by size:
1649 166 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 919 928 1466 1956
Size: 709 210 9 538 490
Fragments arranged by size:
709 538 490 210 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1345 1956
Size: 1345 611
EcoO109I rG'GnC_Cy
Cuts at: 0 616 1956
Size: 616 1340
FspI TGC'GCA
Cuts at: 0 1078 1956
Size: 1078 878
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1180 1212 1346 1378 1956
Size: 1180 32 134 32 578
Fragments arranged by size:
1180 578 134 32 32
HincII GTy'rAC
Cuts at: 0 792 1123 1956
Size: 792 331 833
Fragments arranged by size:
833 792 331
KpnI G_GTAC'C
Cuts at: 0 1313 1956
Size: 1313 643
MfeI C'AATT_G
Cuts at: 0 449 1956
Size: 449 1507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1956
Size: 1008 948
MscI TGG'CCA
Cuts at: 0 840 1092 1956
Size: 840 252 864
Fragments arranged by size:
864 840 252
MslI CAynn'nnrTG
Cuts at: 0 822 1956
Size: 822 1134
MspA1I CmG'CkG
Cuts at: 0 1696 1956
Size: 1696 260
NcoI C'CATG_G
Cuts at: 0 1107 1956
Size: 1107 849
NdeI CA'TA_TG
Cuts at: 0 201 1956
Size: 201 1755
PasI CC'CwG_GG
Cuts at: 0 1333 1956
Size: 1333 623
PflMI CCAn_nnn'nTGG
Cuts at: 0 942 990 1956
Size: 942 48 966
Fragments arranged by size:
966 942 48
PfoI T'CCnGG_A
Cuts at: 0 666 686 1869 1956
Size: 666 20 1183 87
Fragments arranged by size:
1183 666 87 20
PpuMI rG'GwC_Cy
Cuts at: 0 616 1956
Size: 616 1340
PshAI GACnn'nnGTC
Cuts at: 0 1118 1956
Size: 1118 838
PvuII CAG'CTG
Cuts at: 0 1696 1956
Size: 1696 260
SacI G_AGCT'C
Cuts at: 0 143 1792 1956
Size: 143 1649 164
Fragments arranged by size:
1649 164 143
ScaI AGT'ACT
Cuts at: 0 588 1956
Size: 588 1368
SfcI C'TryA_G
Cuts at: 0 1184 1956
Size: 1184 772
SmlI C'TyrA_G
Cuts at: 0 1724 1956
Size: 1724 232
SspI AAT'ATT
Cuts at: 0 1031 1956
Size: 1031 925
StuI AGG'CCT
Cuts at: 0 804 1956
Size: 804 1152
StyI C'CwwG_G
Cuts at: 0 1107 1956
Size: 1107 849
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 901 1185 1538 1956
Size: 901 284 353 418
Fragments arranged by size:
901 418 353 284
TatI w'GTAC_w
Cuts at: 0 540 586 718 1956
Size: 540 46 132 1238
Fragments arranged by size:
1238 540 132 46
XbaI T'CTAG_A
Cuts at: 0 574 1956
Size: 574 1382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1133 1669 1804 1956
Size: 1133 536 135 152
Fragments arranged by size:
1133 536 152 135
ZraI GAC'GTC
Cuts at: 0 673 1956
Size: 673 1283
Enzymes that do cut:
AarI AatII Acc65I AcuI AleI AloI AlwNI ApaLI
ApoI BaeI BanI BanII BbsI BciVI BclI BglI
BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaWI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BstYI BtgI BtsI CspCI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I HincII
KpnI MfeI MmeI MscI MslI MspA1I NcoI NdeI
PasI PflMI PfoI PpuMI PshAI PvuII SacI ScaI
SfcI SmlI SspI StuI StyI TaqII TatI XbaI
XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AlfI
ApaI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BbvCI BcgI BfrBI BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaBI BsaXI BsgI BsiEI BsiWI BspHI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoRI
EcoRV FalI FseI FspAI HaeII HindIII HpaI KasI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TstI Tth111I XhoI
XmaI XmnI