Restriction map for
pF1KB4784
(Linear) (Six-base) MAPSORT of: KIBB4784.seq Check: 2700 from: 1 to: 813
KIBB4784.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AatII G_ACGT'C
Cuts at: 0 782 813
Size: 782 31
AccI GT'mk_AC
Cuts at: 0 782 813
Size: 782 31
AgeI A'CCGG_T
Cuts at: 0 533 813
Size: 533 280
AlwNI CAG_nnn'CTG
Cuts at: 0 694 727 813
Size: 694 33 86
Fragments arranged by size:
694 86 33
ApaI G_GGCC'C
Cuts at: 0 555 810 813
Size: 555 255 3
Fragments arranged by size:
555 255 3
AvaI C'yCGr_G
Cuts at: 0 679 813
Size: 679 134
BanII G_rGCy'C
Cuts at: 0 176 555 810 813
Size: 176 379 255 3
Fragments arranged by size:
379 255 176 3
BbsI GAAGACnn'nnnn_
Cuts at: 0 44 813
Size: 44 769
BbvCI CC'TCA_GC
Cuts at: 0 607 813
Size: 607 206
BglI GCCn_nnn'nGGC
Cuts at: 0 496 813
Size: 496 317
BlpI GC'TnA_GC
Cuts at: 0 763 813
Size: 763 50
Bme1580I G_kGCm'C
Cuts at: 0 555 810 813
Size: 555 255 3
Fragments arranged by size:
555 255 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 217 813
Size: 208 9 596
Fragments arranged by size:
596 208 9
Bpu10I CC'TnA_GC
Cuts at: 0 607 813
Size: 607 206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 813
Size: 406 407
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 813
Size: 75 738
BsaBI GATnn'nnATC
Cuts at: 0 748 813
Size: 748 65
BsaHI Gr'CG_yC
Cuts at: 0 41 779 813
Size: 41 738 34
Fragments arranged by size:
738 41 34
BsaWI w'CCGG_w
Cuts at: 0 533 813
Size: 533 280
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 813
Size: 772 41
BseYI C'CCAG_C
Cuts at: 0 140 813
Size: 140 673
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 813
Size: 718 95
BsiHKAI G_wGCw'C
Cuts at: 0 53 176 422 813
Size: 53 123 246 391
Fragments arranged by size:
391 246 123 53
BsmI GAATG_Cn'
Cuts at: 0 690 813
Size: 690 123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 444 813
Size: 444 369
Bsp1286I G_dGCh'C
Cuts at: 0 53 176 422 555 810 813
Size: 53 123 246 133 255 3
Fragments arranged by size:
255 246 133 123 53 3
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 271 533 813
Size: 271 262 280
Fragments arranged by size:
280 271 262
BsrBI CCG'CTC
Cuts at: 0 767 813
Size: 767 46
BsrDI GCAATG_nn'
Cuts at: 0 203 813
Size: 203 610
BsrFI r'CCGG_y
Cuts at: 0 533 813
Size: 533 280
BssSI C'ACGA_G
Cuts at: 0 95 813
Size: 95 718
BstXI CCAn_nnnn'nTGG
Cuts at: 0 148 813
Size: 148 665
BstYI r'GATC_y
Cuts at: 0 216 251 302 813
Size: 216 35 51 511
Fragments arranged by size:
511 216 51 35
BtsI GCAGTG_nn'
Cuts at: 0 485 695 813
Size: 485 210 118
Fragments arranged by size:
485 210 118
DraIII CAC_nnn'GTG
Cuts at: 0 411 813
Size: 411 402
EaeI y'GGCC_r
Cuts at: 0 150 497 813
Size: 150 347 316
Fragments arranged by size:
347 316 150
EarI CTCTTCn'nnn_
Cuts at: 0 487 813
Size: 487 326
EcoICRI GAG'CTC
Cuts at: 0 174 813
Size: 174 639
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 217 813
Size: 208 9 596
Fragments arranged by size:
596 208 9
EcoO109I rG'GnC_Cy
Cuts at: 0 117 260 603 624 807 813
Size: 117 143 343 21 183 6
Fragments arranged by size:
343 183 143 117 21 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 813
Size: 92 32 689
Fragments arranged by size:
689 92 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 813
Size: 194 619
MscI TGG'CCA
Cuts at: 0 152 499 813
Size: 152 347 314
Fragments arranged by size:
347 314 152
MspA1I CmG'CkG
Cuts at: 0 801 813
Size: 801 12
NspI r_CATG'y
Cuts at: 0 148 813
Size: 148 665
PasI CC'CwG_GG
Cuts at: 0 256 813
Size: 256 557
PfoI T'CCnGG_A
Cuts at: 0 644 813
Size: 644 169
PpuMI rG'GwC_Cy
Cuts at: 0 260 603 624 813
Size: 260 343 21 189
Fragments arranged by size:
343 260 189 21
PspOMI G'GGCC_C
Cuts at: 0 551 806 813
Size: 551 255 7
Fragments arranged by size:
551 255 7
PvuII CAG'CTG
Cuts at: 0 801 813
Size: 801 12
SacI G_AGCT'C
Cuts at: 0 176 813
Size: 176 637
SexAI A'CCwGG_T
Cuts at: 0 407 813
Size: 407 406
SfcI C'TryA_G
Cuts at: 0 383 813
Size: 383 430
SmlI C'TyrA_G
Cuts at: 0 421 813
Size: 421 392
StuI AGG'CCT
Cuts at: 0 65 429 813
Size: 65 364 384
Fragments arranged by size:
384 364 65
StyI C'CwwG_G
Cuts at: 0 792 813
Size: 792 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 813
Size: 37 776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 813
Size: 63 750
XmnI GAAnn'nnTTC
Cuts at: 0 476 813
Size: 476 337
ZraI GAC'GTC
Cuts at: 0 780 813
Size: 780 33
Enzymes that do cut:
AatII AccI AgeI AlwNI ApaI AvaI BanII BbsI
BbvCI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI
BstYI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
Hin4I MmeI MscI MspA1I NspI PasI PfoI PpuMI
PspOMI PvuII SacI SexAI SfcI SmlI StuI StyI
TaqII XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI BsaAI BsaXI
BsiEI BsiWI BspEI BspHI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI