(Linear) (Six-base) MAPSORT of: KIBB4796.seq Check: 6973 from: 1 to: 669 KIBB4796.orf With 182 enzymes: SgfI * July 18, 2007 13:37 .. Acc65I G'GTAC_C Cuts at: 0 589 669 Size: 589 80 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 669 Size: 484 185 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 377 669 Size: 377 292 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 329 361 669 Size: 329 32 308 Fragments arranged by size: 329 308 32 ApoI r'AATT_y Cuts at: 0 92 310 669 Size: 92 218 359 Fragments arranged by size: 359 218 92 AseI AT'TA_AT Cuts at: 0 470 669 Size: 470 199 BanI G'GyrC_C Cuts at: 0 589 669 Size: 589 80 BbsI GAAGACnn'nnnn_ Cuts at: 0 53 669 Size: 53 616 BfrBI ATG'CAT Cuts at: 0 185 669 Size: 185 484 BmgBI CAC'GTC Cuts at: 0 9 669 Size: 9 660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 357 541 669 Size: 37 320 184 128 Fragments arranged by size: 320 184 128 37 BsaHI Gr'CG_yC Cuts at: 0 50 669 Size: 50 619 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 329 359 669 Size: 329 30 310 Fragments arranged by size: 329 310 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 70 669 Size: 70 599 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 401 641 669 Size: 401 240 28 Fragments arranged by size: 401 240 28 BsmBI CGTCTCn'nnnn_ Cuts at: 0 69 669 Size: 69 600 BstXI CCAn_nnnn'nTGG Cuts at: 0 648 669 Size: 648 21 BstYI r'GATC_y Cuts at: 0 368 669 Size: 368 301 EarI CTCTTCn'nnn_ Cuts at: 0 601 617 669 Size: 601 16 52 Fragments arranged by size: 601 52 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 357 484 541 669 Size: 37 320 127 57 128 Fragments arranged by size: 320 128 127 57 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 233 265 669 Size: 233 32 404 Fragments arranged by size: 404 233 32 KpnI G_GTAC'C Cuts at: 0 593 669 Size: 593 76 MslI CAynn'nnrTG Cuts at: 0 359 646 669 Size: 359 287 23 Fragments arranged by size: 359 287 23 NsiI A_TGCA'T Cuts at: 0 187 669 Size: 187 482 PfoI T'CCnGG_A Cuts at: 0 251 669 Size: 251 418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 329 361 669 Size: 329 32 308 Fragments arranged by size: 329 308 32 SfcI C'TryA_G Cuts at: 0 154 669 Size: 154 515 SspI AAT'ATT Cuts at: 0 198 669 Size: 198 471 TatI w'GTAC_w Cuts at: 0 150 425 669 Size: 150 275 244 Fragments arranged by size: 275 244 150 Enzymes that do cut: Acc65I AcuI AlfI AloI ApoI AseI BanI BbsI BfrBI BmgBI BpmI BsaHI BsaXI BseRI BsgI BsmBI BstXI BstYI EarI Eco57MI Hin4I KpnI MslI NsiI PfoI PpiI SfcI SspI TatI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI