Restriction map for
pF1KB4806
(Linear) (Six-base) MAPSORT of: KIBB4806.seq Check: 9713 from: 1 to: 3411
KIBB4806.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1254 3411
Size: 1254 2157
AccI GT'mk_AC
Cuts at: 0 1227 3411
Size: 1227 2184
Acc65I G'GTAC_C
Cuts at: 0 167 466 3411
Size: 167 299 2945
Fragments arranged by size:
2945 299 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 2044 2896 3411
Size: 895 1149 852 515
Fragments arranged by size:
1149 895 852 515
AfeI AGC'GCT
Cuts at: 0 999 3411
Size: 999 2412
AflIII A'CryG_T
Cuts at: 0 2954 3411
Size: 2954 457
AgeI A'CCGG_T
Cuts at: 0 463 3411
Size: 463 2948
AlwNI CAG_nnn'CTG
Cuts at: 0 433 718 873 917 1119 1380 1551 1656
Size: 433 285 155 44 202 261 171 105
Cuts at: 1656 2001 2560 2997 3340 3411
Size: 345 559 437 343 71
Fragments arranged by size:
559 437 433 345 343 285 261 202
171 155 105 71 44
ApaI G_GGCC'C
Cuts at: 0 1048 1830 2369 3411
Size: 1048 782 539 1042
Fragments arranged by size:
1048 1042 782 539
ApoI r'AATT_y
Cuts at: 0 21 3411
Size: 21 3390
AvaI C'yCGr_G
Cuts at: 0 62 188 420 440 1084 3411
Size: 62 126 232 20 644 2327
Fragments arranged by size:
2327 644 232 126 62 20
AvrII C'CTAG_G
Cuts at: 0 2965 3411
Size: 2965 446
BamHI G'GATC_C
Cuts at: 0 851 1432 3411
Size: 851 581 1979
Fragments arranged by size:
1979 851 581
BanI G'GyrC_C
Cuts at: 0 167 209 466 627 3411
Size: 167 42 257 161 2784
Fragments arranged by size:
2784 257 167 161 42
BanII G_rGCy'C
Cuts at: 0 571 794 835 899 964 1048 1830 2369
Size: 571 223 41 64 65 84 782 539
Cuts at: 2369 3411
Size: 1042
Fragments arranged by size:
1042 782 571 539 223 84 65 64
41
BbsI GAAGACnn'nnnn_
Cuts at: 0 952 1790 2533 2548 2957 3411
Size: 952 838 743 15 409 454
Fragments arranged by size:
952 838 743 454 409 15
BbvCI CC'TCA_GC
Cuts at: 0 156 1792 3411
Size: 156 1636 1619
Fragments arranged by size:
1636 1619 156
BfrBI ATG'CAT
Cuts at: 0 1363 3411
Size: 1363 2048
BglI GCCn_nnn'nGGC
Cuts at: 0 443 2405 3411
Size: 443 1962 1006
Fragments arranged by size:
1962 1006 443
BglII A'GATC_T
Cuts at: 0 1479 3411
Size: 1479 1932
BlpI GC'TnA_GC
Cuts at: 0 834 844 1745 3411
Size: 834 10 901 1666
Fragments arranged by size:
1666 901 834 10
Bme1580I G_kGCm'C
Cuts at: 0 1048 1830 2369 3119 3411
Size: 1048 782 539 750 292
Fragments arranged by size:
1048 782 750 539 292
BmrI ACTGGGnnnn_n'
Cuts at: 0 1211 2223 2315 3411
Size: 1211 1012 92 1096
Fragments arranged by size:
1211 1096 1012 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 98 754 1983 3036 3411
Size: 98 656 1229 1053 375
Fragments arranged by size:
1229 1053 656 375 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 165 787 1357 1755 2071 2077 2086
Size: 86 79 622 570 398 316 6 9
Cuts at: 2086 2626 2704 2712 2841 3007 3019 3411
Size: 540 78 8 129 166 12 392
Fragments arranged by size:
622 570 540 398 392 316 166 129
86 79 78 12 9 8 6
Bpu10I CC'TnA_GC
Cuts at: 0 156 1792 3411
Size: 156 1636 1619
Fragments arranged by size:
1636 1619 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 224 2098 2583 3238 3411
Size: 184 40 1874 485 655 173
Fragments arranged by size:
1874 655 485 184 173 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 86 1290 2278 3100 3411
Size: 86 1204 988 822 311
Fragments arranged by size:
1204 988 822 311 86
BsaAI yAC'GTr
Cuts at: 0 2613 3411
Size: 2613 798
BsaBI GATnn'nnATC
Cuts at: 0 2815 3411
Size: 2815 596
BsaHI Gr'CG_yC
Cuts at: 0 335 446 529 3411
Size: 335 111 83 2882
Fragments arranged by size:
2882 335 111 83
BsaWI w'CCGG_w
Cuts at: 0 463 674 3092 3411
Size: 463 211 2418 319
Fragments arranged by size:
2418 463 319 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 2752 2782 3025 3055 3411
Size: 169 30 2553 30 243 30 356
Fragments arranged by size:
2553 356 243 169 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 736 739 985 2908 3411
Size: 187 549 3 246 1923 503
Fragments arranged by size:
1923 549 503 246 187 3
BseYI C'CCAG_C
Cuts at: 0 172 216 604 619 777 793 902 910
Size: 172 44 388 15 158 16 109 8
Cuts at: 910 1112 1275 1544 2035 2514 3411
Size: 202 163 269 491 479 897
Fragments arranged by size:
897 491 479 388 269 202 172 163
158 109 44 16 15 8
BsiHKAI G_wGCw'C
Cuts at: 0 197 412 835 2612 3411
Size: 197 215 423 1777 799
Fragments arranged by size:
1777 799 423 215 197
BsmI GAATG_Cn'
Cuts at: 0 1835 3411
Size: 1835 1576
BsmBI CGTCTCn'nnnn_
Cuts at: 0 326 3411
Size: 326 3085
Bsp1286I G_dGCh'C
Cuts at: 0 197 412 571 794 835 899 964 1048
Size: 197 215 159 223 41 64 65 84
Cuts at: 1048 1830 2369 2612 3119 3411
Size: 782 539 243 507 292
Fragments arranged by size:
782 539 507 292 243 223 215 197
159 84 65 64 41
BspEI T'CCGG_A
Cuts at: 0 674 3411
Size: 674 2737
BspHI T'CATG_A
Cuts at: 0 2486 3411
Size: 2486 925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 155 1254 2644 3411
Size: 68 87 1099 1390 767
Fragments arranged by size:
1390 1099 767 87 68
BsrBI CCG'CTC
Cuts at: 0 138 2079 3411
Size: 138 1941 1332
Fragments arranged by size:
1941 1332 138
BsrDI GCAATG_nn'
Cuts at: 0 2571 3411
Size: 2571 840
BsrFI r'CCGG_y
Cuts at: 0 206 463 761 975 1691 2450 3411
Size: 206 257 298 214 716 759 961
Fragments arranged by size:
961 759 716 298 257 214 206
BssHII G'CGCG_C
Cuts at: 0 2028 3411
Size: 2028 1383
BstEII G'GTnAC_C
Cuts at: 0 1318 3411
Size: 1318 2093
BstXI CCAn_nnnn'nTGG
Cuts at: 0 43 3411
Size: 43 3368
BstYI r'GATC_y
Cuts at: 0 243 851 1432 1479 2459 2917 3411
Size: 243 608 581 47 980 458 494
Fragments arranged by size:
980 608 581 494 458 243 47
Bsu36I CC'TnA_GG
Cuts at: 0 805 1595 3411
Size: 805 790 1816
Fragments arranged by size:
1816 805 790
BtgI C'CryG_G
Cuts at: 0 36 2591 3411
Size: 36 2555 820
Fragments arranged by size:
2555 820 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2111 3411
Size: 2111 1300
BtsI GCAGTG_nn'
Cuts at: 0 845 1074 1668 2286 2674 3411
Size: 845 229 594 618 388 737
Fragments arranged by size:
845 737 618 594 388 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1244 1279 3411
Size: 1244 35 2132
Fragments arranged by size:
2132 1244 35
DraIII CAC_nnn'GTG
Cuts at: 0 2745 3142 3411
Size: 2745 397 269
Fragments arranged by size:
2745 397 269
EaeI y'GGCC_r
Cuts at: 0 587 3411
Size: 587 2824
EarI CTCTTCn'nnn_
Cuts at: 0 1123 1395 1521 1678 1885 3411
Size: 1123 272 126 157 207 1526
Fragments arranged by size:
1526 1123 272 207 157 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3196 3411
Size: 3196 215
EcoICRI GAG'CTC
Cuts at: 0 833 3411
Size: 833 2578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 165 787 895 1357 1755 2044 2071
Size: 86 79 622 108 462 398 289 27
Cuts at: 2071 2077 2086 2626 2704 2712 2841 2896 3007
Size: 6 9 540 78 8 129 55 111
Cuts at: 3007 3019 3411
Size: 12 392
Fragments arranged by size:
622 540 462 398 392 289 129 111
108 86 79 78 55 27 12 9
8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 804 2437 3411
Size: 804 1633 974
Fragments arranged by size:
1633 974 804
EcoO109I rG'GnC_Cy
Cuts at: 0 669 689 870 1088 2365 2366 2430 3158
Size: 669 20 181 218 1277 1 64 728
Cuts at: 3158 3291 3411
Size: 133 120
Fragments arranged by size:
1277 728 669 218 181 133 120 64
20 1
EcoRI G'AATT_C
Cuts at: 0 21 3411
Size: 21 3390
EcoRV GAT'ATC
Cuts at: 0 503 3411
Size: 503 2908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1194 3411
Size: 1194 2217
FspI TGC'GCA
Cuts at: 0 1660 3411
Size: 1660 1751
HaeII r_GCGC'y
Cuts at: 0 739 800 1001 2001 2346 2451 2691 2902
Size: 739 61 201 1000 345 105 240 211
Cuts at: 2902 3411
Size: 509
Fragments arranged by size:
1000 739 509 345 240 211 201 105
61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 1348 1380 1575 1607 1798 1830
Size: 487 32 829 32 195 32 191 32
Cuts at: 1830 2895 2927 3025 3057 3411
Size: 1065 32 98 32 354
Fragments arranged by size:
1065 829 487 354 195 191 98 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1843 3411
Size: 1843 1568
KpnI G_GTAC'C
Cuts at: 0 171 470 3411
Size: 171 299 2941
Fragments arranged by size:
2941 299 171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 2958 3059 3068 3411
Size: 996 1962 101 9 343
Fragments arranged by size:
1962 996 343 101 9
MslI CAynn'nnrTG
Cuts at: 0 1721 2536 2701 3411
Size: 1721 815 165 710
Fragments arranged by size:
1721 815 710 165
MspA1I CmG'CkG
Cuts at: 0 433 917 1116 1147 1749 2467 2732 3411
Size: 433 484 199 31 602 718 265 679
Fragments arranged by size:
718 679 602 484 433 265 199 31
NaeI GCC'GGC
Cuts at: 0 977 2452 3411
Size: 977 1475 959
Fragments arranged by size:
1475 977 959
NcoI C'CATG_G
Cuts at: 0 2591 3411
Size: 2591 820
NdeI CA'TA_TG
Cuts at: 0 1500 3411
Size: 1500 1911
NgoMIV G'CCGG_C
Cuts at: 0 975 2450 3411
Size: 975 1475 961
Fragments arranged by size:
1475 975 961
NruI TCG'CGA
Cuts at: 0 2130 3411
Size: 2130 1281
NsiI A_TGCA'T
Cuts at: 0 1365 3411
Size: 1365 2046
NspI r_CATG'y
Cuts at: 0 549 1314 1734 3411
Size: 549 765 420 1677
Fragments arranged by size:
1677 765 549 420
PasI CC'CwG_GG
Cuts at: 0 2521 3411
Size: 2521 890
PflMI CCAn_nnn'nTGG
Cuts at: 0 433 483 2255 2560 2745 3411
Size: 433 50 1772 305 185 666
Fragments arranged by size:
1772 666 433 305 185 50
PfoI T'CCnGG_A
Cuts at: 0 491 746 3411
Size: 491 255 2665
Fragments arranged by size:
2665 491 255
PmlI CAC'GTG
Cuts at: 0 2613 3411
Size: 2613 798
PpuMI rG'GwC_Cy
Cuts at: 0 689 870 1088 2430 3411
Size: 689 181 218 1342 981
Fragments arranged by size:
1342 981 689 218 181
PspOMI G'GGCC_C
Cuts at: 0 1044 1826 2365 3411
Size: 1044 782 539 1046
Fragments arranged by size:
1046 1044 782 539
PstI C_TGCA'G
Cuts at: 0 166 643 2341 3411
Size: 166 477 1698 1070
Fragments arranged by size:
1698 1070 477 166
PvuII CAG'CTG
Cuts at: 0 433 917 1749 2732 3411
Size: 433 484 832 983 679
Fragments arranged by size:
983 832 679 484 433
SacI G_AGCT'C
Cuts at: 0 835 3411
Size: 835 2576
SanDI GG'GwC_CC
Cuts at: 0 1088 3411
Size: 1088 2323
SapI GCTCTTCn'nnn_
Cuts at: 0 1521 1678 3411
Size: 1521 157 1733
Fragments arranged by size:
1733 1521 157
SbfI CC_TGCA'GG
Cuts at: 0 166 3411
Size: 166 3245
ScaI AGT'ACT
Cuts at: 0 2095 3319 3411
Size: 2095 1224 92
Fragments arranged by size:
2095 1224 92
SfcI C'TryA_G
Cuts at: 0 162 639 921 2337 3411
Size: 162 477 282 1416 1074
Fragments arranged by size:
1416 1074 477 282 162
SgrAI Cr'CCGG_yG
Cuts at: 0 2450 3411
Size: 2450 961
SmaI CCC'GGG
Cuts at: 0 190 422 442 1086 3411
Size: 190 232 20 644 2325
Fragments arranged by size:
2325 644 232 190 20
SmlI C'TyrA_G
Cuts at: 0 199 239 2113 2598 3217 3411
Size: 199 40 1874 485 619 194
Fragments arranged by size:
1874 619 485 199 194 40
StuI AGG'CCT
Cuts at: 0 3175 3411
Size: 3175 236
StyI C'CwwG_G
Cuts at: 0 118 354 956 965 1487 1565 2249 2403
Size: 118 236 602 9 522 78 684 154
Cuts at: 2403 2426 2591 2965 3411
Size: 23 165 374 446
Fragments arranged by size:
684 602 522 446 374 236 165 154
118 78 23 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 282 1254 2192 2939 3411
Size: 49 233 972 938 747 472
Fragments arranged by size:
972 938 747 472 233 49
TatI w'GTAC_w
Cuts at: 0 1607 2093 2384 3317 3411
Size: 1607 486 291 933 94
Fragments arranged by size:
1607 933 486 291 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 1312 1344 2608 2640 2772 2804
Size: 169 32 1111 32 1264 32 132 32
Cuts at: 2804 3411
Size: 607
Fragments arranged by size:
1264 1111 607 169 132 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2818 3411
Size: 2818 593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 43 1426 2838 3411
Size: 43 1383 1412 573
Fragments arranged by size:
1412 1383 573 43
XmaI C'CCGG_G
Cuts at: 0 188 420 440 1084 3411
Size: 188 232 20 644 2327
Fragments arranged by size:
2327 644 232 188 20
XmnI GAAnn'nnTTC
Cuts at: 0 315 2545 3411
Size: 315 2230 866
Fragments arranged by size:
2230 866 315
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HindIII KpnI MmeI MslI
MspA1I NaeI NcoI NdeI NgoMIV NruI NsiI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SbfI ScaI SfcI SgrAI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AhdI AleI AlfI AloI ApaLI
AscI AseI AsiSI BaeI BbeI BcgI BciVI BclI
BmgBI BmtI BsgI BsiEI BsiWI BsrGI BssSI BstAPI
BstBI BstZ17I ClaI DraI DrdI EagI FseI FspAI
HincII HpaI KasI MfeI MluI MscI NarI NheI
NotI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SexAI SfiI SfoI
SgfI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI ZraI