Restriction map for
pF1KB4823
(Linear) (Six-base) MAPSORT of: KIBB4823.seq Check: 5695 from: 1 to: 2223
KIBB4823.orf
With 182 enzymes: SgfI *
January 16, 2014 17:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 582 928 1327 1896 2223
Size: 220 362 346 399 569 327
Fragments arranged by size:
569 399 362 346 327 220
AflII C'TTAA_G
Cuts at: 0 1470 2223
Size: 1470 753
AflIII A'CryG_T
Cuts at: 0 71 2009 2087 2223
Size: 71 1938 78 136
Fragments arranged by size:
1938 136 78 71
AhdI GACnn_n'nnGTC
Cuts at: 0 72 2223
Size: 72 2151
AleI CACnn'nnGTG
Cuts at: 0 43 2223
Size: 43 2180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1066 2223
Size: 1066 1157
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 799 831 2223
Size: 799 32 1392
Fragments arranged by size:
1392 799 32
ApaI G_GGCC'C
Cuts at: 0 227 308 2223
Size: 227 81 1915
Fragments arranged by size:
1915 227 81
ApaLI G'TGCA_C
Cuts at: 0 310 2035 2223
Size: 310 1725 188
Fragments arranged by size:
1725 310 188
AseI AT'TA_AT
Cuts at: 0 1133 2223
Size: 1133 1090
AvaI C'yCGr_G
Cuts at: 0 690 828 857 1746 2223
Size: 690 138 29 889 477
Fragments arranged by size:
889 690 477 138 29
BamHI G'GATC_C
Cuts at: 0 1708 2223
Size: 1708 515
BanI G'GyrC_C
Cuts at: 0 636 2223
Size: 636 1587
BanII G_rGCy'C
Cuts at: 0 115 227 308 367 580 1469 1736 2223
Size: 115 112 81 59 213 889 267 487
Fragments arranged by size:
889 487 267 213 115 112 81 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 934 2223
Size: 934 1289
BbvCI CC'TCA_GC
Cuts at: 0 616 2223
Size: 616 1607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1578 1612 2223
Size: 1578 34 611
Fragments arranged by size:
1578 611 34
BclI T'GATC_A
Cuts at: 0 1257 2223
Size: 1257 966
BglI GCCn_nnn'nGGC
Cuts at: 0 448 457 2134 2223
Size: 448 9 1677 89
Fragments arranged by size:
1677 448 89 9
BlpI GC'TnA_GC
Cuts at: 0 1717 2223
Size: 1717 506
Bme1580I G_kGCm'C
Cuts at: 0 227 308 314 2039 2223
Size: 227 81 6 1725 184
Fragments arranged by size:
1725 227 184 81 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 926 1976 2223
Size: 311 615 1050 247
Fragments arranged by size:
1050 615 311 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1079 1439 2223
Size: 184 895 360 784
Fragments arranged by size:
895 784 360 184
Bpu10I CC'TnA_GC
Cuts at: 0 616 2223
Size: 616 1607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 724 1252 2223
Size: 435 289 528 971
Fragments arranged by size:
971 528 435 289
BsaI GGTCTCn'nnnn_
Cuts at: 0 1403 2223
Size: 1403 820
BsaAI yAC'GTr
Cuts at: 0 573 2223
Size: 573 1650
BsaHI Gr'CG_yC
Cuts at: 0 910 2223
Size: 910 1313
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 739 769 1009 1012 1039 1042
Size: 562 30 147 30 240 3 27 3
Cuts at: 1042 2223
Size: 1181
Fragments arranged by size:
1181 562 240 147 30 30 27 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 650 1017 2223
Size: 181 469 367 1206
Fragments arranged by size:
1206 469 367 181
BseYI C'CCAG_C
Cuts at: 0 653 1504 1980 2169 2223
Size: 653 851 476 189 54
Fragments arranged by size:
851 653 476 189 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 2223
Size: 1444 779
BsiHKAI G_wGCw'C
Cuts at: 0 314 2039 2223
Size: 314 1725 184
Fragments arranged by size:
1725 314 184
Bsp1286I G_dGCh'C
Cuts at: 0 115 227 308 314 367 580 1469 1736
Size: 115 112 81 6 53 213 889 267
Cuts at: 1736 2039 2223
Size: 303 184
Fragments arranged by size:
889 303 267 213 184 115 112 81
53 6
BspHI T'CATG_A
Cuts at: 0 1067 2223
Size: 1067 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 951 1835 2223
Size: 951 884 388
Fragments arranged by size:
951 884 388
BsrBI CCG'CTC
Cuts at: 0 764 1647 2223
Size: 764 883 576
Fragments arranged by size:
883 764 576
BsrDI GCAATG_nn'
Cuts at: 0 183 1881 2223
Size: 183 1698 342
Fragments arranged by size:
1698 342 183
BsrGI T'GTAC_A
Cuts at: 0 1331 2223
Size: 1331 892
BstEII G'GTnAC_C
Cuts at: 0 1006 2223
Size: 1006 1217
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1504 2223
Size: 1504 719
BstYI r'GATC_y
Cuts at: 0 1201 1267 1708 1808 2223
Size: 1201 66 441 100 415
Fragments arranged by size:
1201 441 415 100 66
BtgI C'CryG_G
Cuts at: 0 461 1773 2223
Size: 461 1312 450
Fragments arranged by size:
1312 461 450
BtsI GCAGTG_nn'
Cuts at: 0 1432 1444 1674 2223
Size: 1432 12 230 549
Fragments arranged by size:
1432 549 230 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 114 149 2223
Size: 114 35 2074
Fragments arranged by size:
2074 114 35
EaeI y'GGCC_r
Cuts at: 0 180 348 458 878 2223
Size: 180 168 110 420 1345
Fragments arranged by size:
1345 420 180 168 110
EarI CTCTTCn'nnn_
Cuts at: 0 532 2223
Size: 532 1691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 571 2223
Size: 571 1652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 220 582 928 1079 1327 1439 1896
Size: 184 36 362 346 151 248 112 457
Cuts at: 1896 2223
Size: 327
Fragments arranged by size:
457 362 346 327 248 184 151 112
36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 2223
Size: 166 2057
EcoO109I rG'GnC_Cy
Cuts at: 0 223 470 612 1824 1902 2174 2223
Size: 223 247 142 1212 78 272 49
Fragments arranged by size:
1212 272 247 223 142 78 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 765 1496 2223
Size: 765 731 727
Fragments arranged by size:
765 731 727
FspI TGC'GCA
Cuts at: 0 866 2223
Size: 866 1357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 481 513 739 771 900 932
Size: 58 32 391 32 226 32 129 32
Cuts at: 932 964 996 1009 1041 1193 1225 1700 1732
Size: 32 32 13 32 152 32 475 32
Cuts at: 1732 2194 2223
Size: 462 29
Fragments arranged by size:
475 462 391 226 152 129 58 32
32 32 32 32 32 32 32 32
29 13
HincII GTy'rAC
Cuts at: 0 483 1920 2223
Size: 483 1437 303
Fragments arranged by size:
1437 483 303
HindIII A'AGCT_T
Cuts at: 0 844 2223
Size: 844 1379
MluI A'CGCG_T
Cuts at: 0 2009 2223
Size: 2009 214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1838 2223
Size: 1838 385
MscI TGG'CCA
Cuts at: 0 182 350 460 2223
Size: 182 168 110 1763
Fragments arranged by size:
1763 182 168 110
MslI CAynn'nnrTG
Cuts at: 0 43 334 796 1726 2223
Size: 43 291 462 930 497
Fragments arranged by size:
930 497 462 291 43
MspA1I CmG'CkG
Cuts at: 0 255 608 948 1806 2223
Size: 255 353 340 858 417
Fragments arranged by size:
858 417 353 340 255
NcoI C'CATG_G
Cuts at: 0 461 2223
Size: 461 1762
PfoI T'CCnGG_A
Cuts at: 0 1388 2223
Size: 1388 835
PpuMI rG'GwC_Cy
Cuts at: 0 470 612 1824 2223
Size: 470 142 1212 399
Fragments arranged by size:
1212 470 399 142
PspOMI G'GGCC_C
Cuts at: 0 223 304 2223
Size: 223 81 1919
Fragments arranged by size:
1919 223 81
PstI C_TGCA'G
Cuts at: 0 1451 1669 2223
Size: 1451 218 554
Fragments arranged by size:
1451 554 218
PvuII CAG'CTG
Cuts at: 0 255 948 2223
Size: 255 693 1275
Fragments arranged by size:
1275 693 255
SapI GCTCTTCn'nnn_
Cuts at: 0 532 2223
Size: 532 1691
SfcI C'TryA_G
Cuts at: 0 1115 1447 1511 1665 2223
Size: 1115 332 64 154 558
Fragments arranged by size:
1115 558 332 154 64
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 457 2223
Size: 457 1766
SmaI CCC'GGG
Cuts at: 0 859 2223
Size: 859 1364
SmlI C'TyrA_G
Cuts at: 0 414 739 1231 1470 1746 2223
Size: 414 325 492 239 276 477
Fragments arranged by size:
492 477 414 325 276 239
SrfI GCCC'GGGC
Cuts at: 0 859 2223
Size: 859 1364
StuI AGG'CCT
Cuts at: 0 412 2223
Size: 412 1811
StyI C'CwwG_G
Cuts at: 0 80 461 851 1097 1497 2132 2223
Size: 80 381 390 246 400 635 91
Fragments arranged by size:
635 400 390 381 246 91 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1030 1426 1547 2169 2223
Size: 1030 396 121 622 54
Fragments arranged by size:
1030 622 396 121 54
TatI w'GTAC_w
Cuts at: 0 281 1331 2015 2223
Size: 281 1050 684 208
Fragments arranged by size:
1050 684 281 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 974 1006 2223
Size: 974 32 1217
Fragments arranged by size:
1217 974 32
Tth111I GACn'n_nGTC
Cuts at: 0 474 976 2223
Size: 474 502 1247
Fragments arranged by size:
1247 502 474
XhoI C'TCGA_G
Cuts at: 0 1746 2223
Size: 1746 477
XmaI C'CCGG_G
Cuts at: 0 857 2223
Size: 857 1366
Enzymes that do cut:
AcuI AflII AflIII AhdI AleI AlfI AloI ApaI
ApaLI AseI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstEII
BstXI BstYI BtgI BtsI CspCI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI FspI Hin4I HincII HindIII
MluI MmeI MscI MslI MspA1I NcoI PfoI PpuMI
PspOMI PstI PvuII SapI SfcI SfiI SmaI SmlI
SrfI StuI StyI TaqII TatI TstI Tth111I XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AlwNI
ApoI AscI AsiSI AvrII BaeI BbeI BciVI BfrBI
BglII BmgBI BmtI BplI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FseI FspAI HaeII HpaI KasI
KpnI MfeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI XbaI
XcmI XmnI ZraI