Restriction map for
pF1KB4835
(Linear) (Six-base) MAPSORT of: KIBB4835.seq Check: 1747 from: 1 to: 1434
KIBB4835.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 683 744 1139 1434
Size: 683 61 395 295
Fragments arranged by size:
683 395 295 61
Acc65I G'GTAC_C
Cuts at: 0 526 1434
Size: 526 908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 851 1339 1434
Size: 526 325 488 95
Fragments arranged by size:
526 488 325 95
AflIII A'CryG_T
Cuts at: 0 725 1434
Size: 725 709
AleI CACnn'nnGTG
Cuts at: 0 46 1434
Size: 46 1388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1434
Size: 1216 32 186
Fragments arranged by size:
1216 186 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1227 1434
Size: 1227 207
ApaI G_GGCC'C
Cuts at: 0 164 1434
Size: 164 1270
ApoI r'AATT_y
Cuts at: 0 403 1434
Size: 403 1031
AvaI C'yCGr_G
Cuts at: 0 353 657 1196 1434
Size: 353 304 539 238
Fragments arranged by size:
539 353 304 238
BanI G'GyrC_C
Cuts at: 0 37 526 1127 1434
Size: 37 489 601 307
Fragments arranged by size:
601 489 307 37
BanII G_rGCy'C
Cuts at: 0 164 353 658 1203 1434
Size: 164 189 305 545 231
Fragments arranged by size:
545 305 231 189 164
BbvCI CC'TCA_GC
Cuts at: 0 64 1434
Size: 64 1370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 233 574 608 1434
Size: 199 34 341 34 826
Fragments arranged by size:
826 341 199 34 34
BfrBI ATG'CAT
Cuts at: 0 279 1434
Size: 279 1155
BglI GCCn_nnn'nGGC
Cuts at: 0 168 1434
Size: 168 1266
BglII A'GATC_T
Cuts at: 0 1017 1434
Size: 1017 417
Bme1580I G_kGCm'C
Cuts at: 0 40 117 164 1190 1434
Size: 40 77 47 1026 244
Fragments arranged by size:
1026 244 77 47 40
BmgBI CAC'GTC
Cuts at: 0 1314 1434
Size: 1314 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 901 1434
Size: 901 533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1048 1240 1434
Size: 875 173 192 194
Fragments arranged by size:
875 194 192 173
Bpu10I CC'TnA_GC
Cuts at: 0 64 1434
Size: 64 1370
BsaAI yAC'GTr
Cuts at: 0 377 1191 1434
Size: 377 814 243
Fragments arranged by size:
814 377 243
BsaWI w'CCGG_w
Cuts at: 0 242 1406 1434
Size: 242 1164 28
Fragments arranged by size:
1164 242 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 224 254 645 675 1216 1246 1434
Size: 224 30 391 30 541 30 188
Fragments arranged by size:
541 391 224 188 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 666 1155 1158 1434
Size: 666 489 3 276
Fragments arranged by size:
666 489 276 3
BseYI C'CCAG_C
Cuts at: 0 236 422 602 1116 1434
Size: 236 186 180 514 318
Fragments arranged by size:
514 318 236 186 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1096 1213 1351 1434
Size: 866 230 117 138 83
Fragments arranged by size:
866 230 138 117 83
BsiEI CG_ry'CG
Cuts at: 0 13 143 1434
Size: 13 130 1291
Fragments arranged by size:
1291 130 13
BsiHKAI G_wGCw'C
Cuts at: 0 658 1434
Size: 658 776
BsmI GAATG_Cn'
Cuts at: 0 597 1434
Size: 597 837
Bsp1286I G_dGCh'C
Cuts at: 0 40 117 164 353 658 1190 1203 1434
Size: 40 77 47 189 305 532 13 231
Fragments arranged by size:
532 305 231 189 77 47 40 13
BspEI T'CCGG_A
Cuts at: 0 242 1406 1434
Size: 242 1164 28
Fragments arranged by size:
1164 242 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 744 1139 1235 1434
Size: 683 61 395 96 199
Fragments arranged by size:
683 395 199 96 61
BsrDI GCAATG_nn'
Cuts at: 0 978 1434
Size: 978 456
BsrFI r'CCGG_y
Cuts at: 0 523 1434
Size: 523 911
BssSI C'ACGA_G
Cuts at: 0 208 1434
Size: 208 1226
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1236 1329 1434
Size: 1236 93 105
Fragments arranged by size:
1236 105 93
BstXI CCAn_nnnn'nTGG
Cuts at: 0 280 1434
Size: 280 1154
BstYI r'GATC_y
Cuts at: 0 1017 1434
Size: 1017 417
BtgI C'CryG_G
Cuts at: 0 449 1434
Size: 449 985
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 672 1434
Size: 672 762
BtsI GCAGTG_nn'
Cuts at: 0 104 708 1293 1434
Size: 104 604 585 141
Fragments arranged by size:
604 585 141 104
EaeI y'GGCC_r
Cuts at: 0 10 155 387 446 950 980 1434
Size: 10 145 232 59 504 30 454
Fragments arranged by size:
504 454 232 145 59 30 10
EagI C'GGCC_G
Cuts at: 0 10 1434
Size: 10 1424
EcoICRI GAG'CTC
Cuts at: 0 656 1434
Size: 656 778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 851 875 1048 1240 1339 1434
Size: 526 325 24 173 192 99 95
Fragments arranged by size:
526 325 192 173 99 95 24
EcoO109I rG'GnC_Cy
Cuts at: 0 161 935 1434
Size: 161 774 499
Fragments arranged by size:
774 499 161
EcoRI G'AATT_C
Cuts at: 0 403 1434
Size: 403 1031
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 224 256 1002 1034 1185 1217
Size: 70 32 122 32 746 32 151 32
Cuts at: 1217 1434
Size: 217
Fragments arranged by size:
746 217 151 122 70 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 530 1434
Size: 530 904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1196 1434
Size: 608 588 238
Fragments arranged by size:
608 588 238
MscI TGG'CCA
Cuts at: 0 448 982 1434
Size: 448 534 452
Fragments arranged by size:
534 452 448
MslI CAynn'nnrTG
Cuts at: 0 46 262 440 539 1434
Size: 46 216 178 99 895
Fragments arranged by size:
895 216 178 99 46
MspA1I CmG'CkG
Cuts at: 0 602 849 1072 1143 1381 1434
Size: 602 247 223 71 238 53
Fragments arranged by size:
602 247 238 223 71 53
NsiI A_TGCA'T
Cuts at: 0 281 1434
Size: 281 1153
NspI r_CATG'y
Cuts at: 0 729 999 1434
Size: 729 270 435
Fragments arranged by size:
729 435 270
PasI CC'CwG_GG
Cuts at: 0 165 1182 1434
Size: 165 1017 252
Fragments arranged by size:
1017 252 165
PciI A'CATG_T
Cuts at: 0 725 1434
Size: 725 709
PflMI CCAn_nnn'nTGG
Cuts at: 0 476 999 1434
Size: 476 523 435
Fragments arranged by size:
523 476 435
PfoI T'CCnGG_A
Cuts at: 0 1256 1303 1434
Size: 1256 47 131
Fragments arranged by size:
1256 131 47
PmlI CAC'GTG
Cuts at: 0 377 1191 1434
Size: 377 814 243
Fragments arranged by size:
814 377 243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1202 1216 1234 1248 1434
Size: 1202 14 18 14 186
Fragments arranged by size:
1202 186 18 14 14
PpuMI rG'GwC_Cy
Cuts at: 0 935 1434
Size: 935 499
PspOMI G'GGCC_C
Cuts at: 0 160 1434
Size: 160 1274
PvuII CAG'CTG
Cuts at: 0 602 849 1072 1143 1381 1434
Size: 602 247 223 71 238 53
Fragments arranged by size:
602 247 238 223 71 53
SacI G_AGCT'C
Cuts at: 0 658 1434
Size: 658 776
SexAI A'CCwGG_T
Cuts at: 0 368 1434
Size: 368 1066
SmaI CCC'GGG
Cuts at: 0 1198 1434
Size: 1198 236
StuI AGG'CCT
Cuts at: 0 784 1025 1434
Size: 784 241 409
Fragments arranged by size:
784 409 241
StyI C'CwwG_G
Cuts at: 0 306 767 1434
Size: 306 461 667
Fragments arranged by size:
667 461 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 760 1434
Size: 760 674
XmaI C'CCGG_G
Cuts at: 0 1196 1434
Size: 1196 238
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbvCI BcgI BfrBI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI EaeI EagI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I KpnI MmeI MscI MslI MspA1I NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PvuII SacI SexAI SmaI StuI StyI TaqII
XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BciVI BclI BlpI BmtI BplI BpuEI BsaI
BsaBI BsaHI BsiWI BsmBI BspHI BsrBI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EarI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PmeI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI