Restriction map for
pF1KB4848
(Linear) (Six-base) MAPSORT of: KIBB4848.seq Check: 5635 from: 1 to: 1257
KIBB4848.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
Acc65I G'GTAC_C
Cuts at: 0 215 1257
Size: 215 1042
AfeI AGC'GCT
Cuts at: 0 154 1257
Size: 154 1103
AgeI A'CCGG_T
Cuts at: 0 212 1257
Size: 212 1045
AleI CACnn'nnGTG
Cuts at: 0 241 1257
Size: 241 1016
AlwNI CAG_nnn'CTG
Cuts at: 0 1085 1192 1257
Size: 1085 107 65
Fragments arranged by size:
1085 107 65
ApaLI G'TGCA_C
Cuts at: 0 184 1257
Size: 184 1073
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 207 240 962 995 1257
Size: 207 33 722 33 262
Fragments arranged by size:
722 262 207 33 33
BanI G'GyrC_C
Cuts at: 0 215 1257
Size: 215 1042
BanII G_rGCy'C
Cuts at: 0 526 853 961 1217 1252 1257
Size: 526 327 108 256 35 5
Fragments arranged by size:
526 327 256 108 35 5
BbsI GAAGACnn'nnnn_
Cuts at: 0 267 1257
Size: 267 990
BbvCI CC'TCA_GC
Cuts at: 0 1169 1257
Size: 1169 88
BciVI GTATCCnnnnn_n'
Cuts at: 0 963 1257
Size: 963 294
BclI T'GATC_A
Cuts at: 0 821 1257
Size: 821 436
BglI GCCn_nnn'nGGC
Cuts at: 0 1221 1257
Size: 1221 36
BlpI GC'TnA_GC
Cuts at: 0 875 1193 1257
Size: 875 318 64
Fragments arranged by size:
875 318 64
Bme1580I G_kGCm'C
Cuts at: 0 188 1257
Size: 188 1069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1257
Size: 1114 143
Bpu10I CC'TnA_GC
Cuts at: 0 1169 1257
Size: 1169 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1257
Size: 235 1022
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 427 586 1060 1257
Size: 199 228 159 474 197
Fragments arranged by size:
474 228 199 197 159
BsaWI w'CCGG_w
Cuts at: 0 212 1257
Size: 212 1045
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 806 1117 1257
Size: 490 316 311 140
Fragments arranged by size:
490 316 311 140
BseYI C'CCAG_C
Cuts at: 0 1078 1141 1155 1224 1257
Size: 1078 63 14 69 33
Fragments arranged by size:
1078 69 63 33 14
BsiEI CG_ry'CG
Cuts at: 0 798 1257
Size: 798 459
BsiHKAI G_wGCw'C
Cuts at: 0 188 961 983 1257
Size: 188 773 22 274
Fragments arranged by size:
773 274 188 22
BsiWI C'GTAC_G
Cuts at: 0 115 1257
Size: 115 1142
BsmI GAATG_Cn'
Cuts at: 0 24 1257
Size: 24 1233
Bsp1286I G_dGCh'C
Cuts at: 0 188 526 853 961 983 1217 1252 1257
Size: 188 338 327 108 22 234 35 5
Fragments arranged by size:
338 327 234 188 108 35 22 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 952 1257
Size: 952 305
BsrBI CCG'CTC
Cuts at: 0 170 589 1257
Size: 170 419 668
Fragments arranged by size:
668 419 170
BsrDI GCAATG_nn'
Cuts at: 0 101 329 1257
Size: 101 228 928
Fragments arranged by size:
928 228 101
BsrFI r'CCGG_y
Cuts at: 0 212 1121 1257
Size: 212 909 136
Fragments arranged by size:
909 212 136
BssSI C'ACGA_G
Cuts at: 0 832 1257
Size: 832 425
BstAPI GCAn_nnn'nTGC
Cuts at: 0 944 1257
Size: 944 313
BstEII G'GTnAC_C
Cuts at: 0 346 1257
Size: 346 911
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 1098 1224 1257
Size: 243 855 126 33
Fragments arranged by size:
855 243 126 33
BtgI C'CryG_G
Cuts at: 0 189 281 1257
Size: 189 92 976
Fragments arranged by size:
976 189 92
BtsI GCAGTG_nn'
Cuts at: 0 1150 1257
Size: 1150 107
DraIII CAC_nnn'GTG
Cuts at: 0 687 1257
Size: 687 570
EaeI y'GGCC_r
Cuts at: 0 128 1257
Size: 128 1129
EarI CTCTTCn'nnn_
Cuts at: 0 160 235 577 808 841 884 1105 1257
Size: 160 75 342 231 33 43 221 152
Fragments arranged by size:
342 231 221 160 152 75 43 33
EcoICRI GAG'CTC
Cuts at: 0 959 1257
Size: 959 298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1257
Size: 1114 143
EcoO109I rG'GnC_Cy
Cuts at: 0 60 1257
Size: 60 1197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 1257
Size: 276 981
HaeII r_GCGC'y
Cuts at: 0 156 261 1257
Size: 156 105 996
Fragments arranged by size:
996 156 105
KpnI G_GTAC'C
Cuts at: 0 219 1257
Size: 219 1038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1257
Size: 632 625
MslI CAynn'nnrTG
Cuts at: 0 241 625 772 937 1257
Size: 241 384 147 165 320
Fragments arranged by size:
384 320 241 165 147
MspA1I CmG'CkG
Cuts at: 0 879 1082 1257
Size: 879 203 175
Fragments arranged by size:
879 203 175
NaeI GCC'GGC
Cuts at: 0 1123 1257
Size: 1123 134
NcoI C'CATG_G
Cuts at: 0 281 1257
Size: 281 976
NgoMIV G'CCGG_C
Cuts at: 0 1121 1257
Size: 1121 136
NspI r_CATG'y
Cuts at: 0 558 1257
Size: 558 699
PasI CC'CwG_GG
Cuts at: 0 56 1257
Size: 56 1201
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 687 1257
Size: 36 651 570
Fragments arranged by size:
651 570 36
PfoI T'CCnGG_A
Cuts at: 0 200 1257
Size: 200 1057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 1257
Size: 727 32 498
Fragments arranged by size:
727 498 32
PpuMI rG'GwC_Cy
Cuts at: 0 60 1257
Size: 60 1197
PvuII CAG'CTG
Cuts at: 0 879 1082 1257
Size: 879 203 175
Fragments arranged by size:
879 203 175
SacI G_AGCT'C
Cuts at: 0 961 1257
Size: 961 296
SanDI GG'GwC_CC
Cuts at: 0 60 1257
Size: 60 1197
SapI GCTCTTCn'nnn_
Cuts at: 0 160 841 1257
Size: 160 681 416
Fragments arranged by size:
681 416 160
SmlI C'TyrA_G
Cuts at: 0 250 1257
Size: 250 1007
StuI AGG'CCT
Cuts at: 0 694 1257
Size: 694 563
StyI C'CwwG_G
Cuts at: 0 281 1013 1091 1217 1257
Size: 281 732 78 126 40
Fragments arranged by size:
732 281 126 78 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 1092 1216 1257
Size: 451 641 124 41
Fragments arranged by size:
641 451 124 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 369 401 1257
Size: 238 32 99 32 856
Fragments arranged by size:
856 238 99 32 32
XbaI T'CTAG_A
Cuts at: 0 912 1257
Size: 912 345
Enzymes that do cut:
Acc65I AfeI AgeI AleI AlwNI ApaLI BaeI BanI
BanII BbsI BbvCI BciVI BclI BglI BlpI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII KpnI MmeI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PvuII SacI SanDI SapI SmlI
StuI StyI TaqII TstI XbaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AflIII AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BcgI BfrBI BglII BmgBI BmrI
BmtI BplI BsaAI BsaBI BsaHI BsaXI BsgI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI MfeI MluI MscI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XcmI XhoI XmaI XmnI ZraI