Restriction map for
pF1KB4865
(Linear) (Six-base) MAPSORT of: KIBB4865.seq Check: 6578 from: 1 to: 2769
KIBB4865.orf
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 0 983 1262 2769
Size: 983 279 1507
Fragments arranged by size:
1507 983 279
Acc65I G'GTAC_C
Cuts at: 0 110 2769
Size: 110 2659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 2769
Size: 326 2443
AfeI AGC'GCT
Cuts at: 0 913 2769
Size: 913 1856
AflIII A'CryG_T
Cuts at: 0 2180 2769
Size: 2180 589
AleI CACnn'nnGTG
Cuts at: 0 149 1873 2276 2769
Size: 149 1724 403 493
Fragments arranged by size:
1724 493 403 149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1891 2769
Size: 1891 878
AlwNI CAG_nnn'CTG
Cuts at: 0 242 1367 2769
Size: 242 1125 1402
Fragments arranged by size:
1402 1125 242
ApoI r'AATT_y
Cuts at: 0 364 416 562 854 1951 2769
Size: 364 52 146 292 1097 818
Fragments arranged by size:
1097 818 364 292 146 52
AseI AT'TA_AT
Cuts at: 0 2399 2769
Size: 2399 370
AvaI C'yCGr_G
Cuts at: 0 106 1696 2769
Size: 106 1590 1073
Fragments arranged by size:
1590 1073 106
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2487 2520 2769
Size: 2487 33 249
Fragments arranged by size:
2487 249 33
BanI G'GyrC_C
Cuts at: 0 110 1027 1867 2769
Size: 110 917 840 902
Fragments arranged by size:
917 902 840 110
BanII G_rGCy'C
Cuts at: 0 1727 1966 2131 2769
Size: 1727 239 165 638
Fragments arranged by size:
1727 638 239 165
BbeI G_GCGC'C
Cuts at: 0 1031 2769
Size: 1031 1738
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 332 1106 2560 2769
Size: 258 74 774 1454 209
Fragments arranged by size:
1454 774 258 209 74
BciVI GTATCCnnnnn_n'
Cuts at: 0 968 2488 2769
Size: 968 1520 281
Fragments arranged by size:
1520 968 281
BclI T'GATC_A
Cuts at: 0 203 1583 2769
Size: 203 1380 1186
Fragments arranged by size:
1380 1186 203
Bme1580I G_kGCm'C
Cuts at: 0 1416 2598 2769
Size: 1416 1182 171
Fragments arranged by size:
1416 1182 171
BmgBI CAC'GTC
Cuts at: 0 2196 2769
Size: 2196 573
BmrI ACTGGGnnnn_n'
Cuts at: 0 879 1729 1995 2769
Size: 879 850 266 774
Fragments arranged by size:
879 850 774 266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 606 1724 2769
Size: 606 1118 1045
Fragments arranged by size:
1118 1045 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 601 2514 2707 2769
Size: 584 17 1913 193 62
Fragments arranged by size:
1913 584 193 62 17
Bpu10I CC'TnA_GC
Cuts at: 0 1646 2769
Size: 1646 1123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1996 2769
Size: 1996 773
BsaAI yAC'GTr
Cuts at: 0 258 2004 2769
Size: 258 1746 765
Fragments arranged by size:
1746 765 258
BsaBI GATnn'nnATC
Cuts at: 0 714 2769
Size: 714 2055
BsaHI Gr'CG_yC
Cuts at: 0 1028 2769
Size: 1028 1741
BsaWI w'CCGG_w
Cuts at: 0 180 2769
Size: 180 2589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1072 1102 2769
Size: 1072 30 1667
Fragments arranged by size:
1667 1072 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 317 1486 2251 2769
Size: 10 307 1169 765 518
Fragments arranged by size:
1169 765 518 307 10
BseYI C'CCAG_C
Cuts at: 0 1409 1740 1958 2769
Size: 1409 331 218 811
Fragments arranged by size:
1409 811 331 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 2026 2769
Size: 413 1613 743
Fragments arranged by size:
1613 743 413
BsiEI CG_ry'CG
Cuts at: 0 56 627 2769
Size: 56 571 2142
Fragments arranged by size:
2142 571 56
BsiHKAI G_wGCw'C
Cuts at: 0 35 2488 2769
Size: 35 2453 281
Fragments arranged by size:
2453 281 35
BsmI GAATG_Cn'
Cuts at: 0 520 722 2769
Size: 520 202 2047
Fragments arranged by size:
2047 520 202
Bsp1286I G_dGCh'C
Cuts at: 0 35 1416 1727 1966 2131 2488 2598 2769
Size: 35 1381 311 239 165 357 110 171
Fragments arranged by size:
1381 357 311 239 171 165 110 35
BspEI T'CCGG_A
Cuts at: 0 180 2769
Size: 180 2589
BspHI T'CATG_A
Cuts at: 0 1532 2769
Size: 1532 1237
BsrBI CCG'CTC
Cuts at: 0 18 2769
Size: 18 2751
BsrDI GCAATG_nn'
Cuts at: 0 2550 2769
Size: 2550 219
BsrFI r'CCGG_y
Cuts at: 0 55 626 2769
Size: 55 571 2143
Fragments arranged by size:
2143 571 55
BstEII G'GTnAC_C
Cuts at: 0 1392 2278 2769
Size: 1392 886 491
Fragments arranged by size:
1392 886 491
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1878 2769
Size: 1878 891
BstYI r'GATC_y
Cuts at: 0 1688 2769
Size: 1688 1081
BstZ17I GTA'TAC
Cuts at: 0 1263 2769
Size: 1263 1506
BtgI C'CryG_G
Cuts at: 0 731 1895 2769
Size: 731 1164 874
Fragments arranged by size:
1164 874 731
BtsI GCAGTG_nn'
Cuts at: 0 807 2157 2769
Size: 807 1350 612
Fragments arranged by size:
1350 807 612
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1149 1184 2769
Size: 1149 35 1585
Fragments arranged by size:
1585 1149 35
DraIII CAC_nnn'GTG
Cuts at: 0 391 463 2769
Size: 391 72 2306
Fragments arranged by size:
2306 391 72
EaeI y'GGCC_r
Cuts at: 0 53 1880 2261 2769
Size: 53 1827 381 508
Fragments arranged by size:
1827 508 381 53
EagI C'GGCC_G
Cuts at: 0 53 2769
Size: 53 2716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1375 1730 2769
Size: 1375 355 1039
Fragments arranged by size:
1375 1039 355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 584 601 2514 2707 2769
Size: 326 258 17 1913 193 62
Fragments arranged by size:
1913 326 258 193 62 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1468 2769
Size: 1468 1301
EcoO109I rG'GnC_Cy
Cuts at: 0 430 2041 2189 2604 2769
Size: 430 1611 148 415 165
Fragments arranged by size:
1611 430 415 165 148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2383 2769
Size: 2383 386
HaeII r_GCGC'y
Cuts at: 0 62 915 1031 2769
Size: 62 853 116 1738
Fragments arranged by size:
1738 853 116 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 309 341 600 632 1072 1104
Size: 162 32 115 32 259 32 440 32
Cuts at: 1104 1709 1741 2769
Size: 605 32 1028
Fragments arranged by size:
1028 605 440 259 162 115 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2027 2769
Size: 2027 742
HindIII A'AGCT_T
Cuts at: 0 570 2769
Size: 570 2199
KasI G'GCGC_C
Cuts at: 0 1027 2769
Size: 1027 1742
KpnI G_GTAC'C
Cuts at: 0 114 2769
Size: 114 2655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1373 1954 2769
Size: 1115 258 581 815
Fragments arranged by size:
1115 815 581 258
MscI TGG'CCA
Cuts at: 0 1882 2263 2769
Size: 1882 381 506
Fragments arranged by size:
1882 506 381
MslI CAynn'nnrTG
Cuts at: 0 149 1246 1873 2276 2587 2769
Size: 149 1097 627 403 311 182
Fragments arranged by size:
1097 627 403 311 182 149
MspA1I CmG'CkG
Cuts at: 0 1768 2250 2769
Size: 1768 482 519
Fragments arranged by size:
1768 519 482
NaeI GCC'GGC
Cuts at: 0 57 2769
Size: 57 2712
NarI GG'CG_CC
Cuts at: 0 1028 2769
Size: 1028 1741
NcoI C'CATG_G
Cuts at: 0 731 2769
Size: 731 2038
NdeI CA'TA_TG
Cuts at: 0 1935 2769
Size: 1935 834
NgoMIV G'CCGG_C
Cuts at: 0 55 2769
Size: 55 2714
NspI r_CATG'y
Cuts at: 0 2184 2769
Size: 2184 585
PasI CC'CwG_GG
Cuts at: 0 2598 2769
Size: 2598 171
PciI A'CATG_T
Cuts at: 0 2180 2769
Size: 2180 589
PflMI CCAn_nnn'nTGG
Cuts at: 0 1936 2769
Size: 1936 833
PfoI T'CCnGG_A
Cuts at: 0 557 948 2530 2769
Size: 557 391 1582 239
Fragments arranged by size:
1582 557 391 239
PmlI CAC'GTG
Cuts at: 0 2004 2769
Size: 2004 765
PpuMI rG'GwC_Cy
Cuts at: 0 430 2041 2189 2604 2769
Size: 430 1611 148 415 165
Fragments arranged by size:
1611 430 415 165 148
PstI C_TGCA'G
Cuts at: 0 909 2769
Size: 909 1860
PvuII CAG'CTG
Cuts at: 0 1768 2250 2769
Size: 1768 482 519
Fragments arranged by size:
1768 519 482
SanDI GG'GwC_CC
Cuts at: 0 2041 2189 2769
Size: 2041 148 580
Fragments arranged by size:
2041 580 148
ScaI AGT'ACT
Cuts at: 0 2754 2769
Size: 2754 15
SexAI A'CCwGG_T
Cuts at: 0 696 2769
Size: 696 2073
SfcI C'TryA_G
Cuts at: 0 783 905 1763 2769
Size: 783 122 858 1006
Fragments arranged by size:
1006 858 783 122
SfoI GGC'GCC
Cuts at: 0 1029 2769
Size: 1029 1740
SmaI CCC'GGG
Cuts at: 0 108 2769
Size: 108 2661
SmlI C'TyrA_G
Cuts at: 0 2011 2769
Size: 2011 758
SspI AAT'ATT
Cuts at: 0 1057 2769
Size: 1057 1712
StyI C'CwwG_G
Cuts at: 0 731 1353 2274 2367 2769
Size: 731 622 921 93 402
Fragments arranged by size:
921 731 622 402 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 161 929 1788 2769
Size: 161 768 859 981
Fragments arranged by size:
981 859 768 161
TatI w'GTAC_w
Cuts at: 0 2636 2752 2769
Size: 2636 116 17
Fragments arranged by size:
2636 116 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1167 1791 1861 2595 2769
Size: 1167 624 70 734 174
Fragments arranged by size:
1167 734 624 174 70
XmaI C'CCGG_G
Cuts at: 0 106 2769
Size: 106 2663
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApoI AseI AvaI BaeI BanI BanII BbeI BbsI
BciVI BclI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BstEII BstXI BstYI BstZ17I BtgI BtsI
CspCI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmlI PpuMI PstI
PvuII SanDI ScaI SexAI SfcI SfoI SmaI SmlI
SspI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AloI ApaI
ApaLI AscI AsiSI AvrII BamHI BbvCI BcgI BfrBI
BglI BglII BlpI BmtI BsaI BsiWI BsmBI BspMI
BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgZI ClaI
DraI DrdI EarI EcoICRI EcoRI EcoRV FseI FspI
FspAI HpaI MfeI MluI NheI NotI NruI NsiI
PacI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI