Restriction map for
pF1KB4879
(Linear) (Six-base) MAPSORT of: KIBB4879.seq Check: 9675 from: 1 to: 2430
KIBB4879.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1692 2430
Size: 1692 738
AatII G_ACGT'C
Cuts at: 0 951 2102 2430
Size: 951 1151 328
Fragments arranged by size:
1151 951 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 904 1333 1802 2430
Size: 149 755 429 469 628
Fragments arranged by size:
755 628 469 429 149
AflIII A'CryG_T
Cuts at: 0 1451 2278 2378 2430
Size: 1451 827 100 52
Fragments arranged by size:
1451 827 100 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1577 2430
Size: 1577 853
AlwNI CAG_nnn'CTG
Cuts at: 0 127 156 403 1642 2430
Size: 127 29 247 1239 788
Fragments arranged by size:
1239 788 247 127 29
ApoI r'AATT_y
Cuts at: 0 36 349 840 1495 1516 2430
Size: 36 313 491 655 21 914
Fragments arranged by size:
914 655 491 313 36 21
AvrII C'CTAG_G
Cuts at: 0 1236 2430
Size: 1236 1194
BamHI G'GATC_C
Cuts at: 0 1510 2430
Size: 1510 920
BanII G_rGCy'C
Cuts at: 0 1829 2430
Size: 1829 601
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 749 899 910 1802 2041 2430
Size: 197 552 150 11 892 239 389
Fragments arranged by size:
892 552 389 239 197 150 11
BbvCI CC'TCA_GC
Cuts at: 0 98 2430
Size: 98 2332
BclI T'GATC_A
Cuts at: 0 2300 2328 2430
Size: 2300 28 102
Fragments arranged by size:
2300 102 28
BlpI GC'TnA_GC
Cuts at: 0 1817 2430
Size: 1817 613
BmrI ACTGGGnnnn_n'
Cuts at: 0 1018 1397 2430
Size: 1018 379 1033
Fragments arranged by size:
1033 1018 379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1379 2430
Size: 1118 261 1051
Fragments arranged by size:
1118 1051 261
Bpu10I CC'TnA_GC
Cuts at: 0 98 473 689 972 1139 1808 2430
Size: 98 375 216 283 167 669 622
Fragments arranged by size:
669 622 375 283 216 167 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1523 2430
Size: 1180 343 907
Fragments arranged by size:
1180 907 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 2113 2209 2404 2430
Size: 2113 96 195 26
Fragments arranged by size:
2113 195 96 26
BsaAI yAC'GTr
Cuts at: 0 2281 2430
Size: 2281 149
BsaBI GATnn'nnATC
Cuts at: 0 1467 2430
Size: 1467 963
BsaHI Gr'CG_yC
Cuts at: 0 948 1615 1718 2099 2430
Size: 948 667 103 381 331
Fragments arranged by size:
948 667 381 331 103
BsaWI w'CCGG_w
Cuts at: 0 408 2430
Size: 408 2022
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 240 1879 2430
Size: 240 1639 551
Fragments arranged by size:
1639 551 240
BseYI C'CCAG_C
Cuts at: 0 876 2430
Size: 876 1554
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1664 2430
Size: 476 1188 766
Fragments arranged by size:
1188 766 476
BsiHKAI G_wGCw'C
Cuts at: 0 538 1566 2430
Size: 538 1028 864
Fragments arranged by size:
1028 864 538
BsmI GAATG_Cn'
Cuts at: 0 1170 2430
Size: 1170 1260
Bsp1286I G_dGCh'C
Cuts at: 0 538 1566 1829 2430
Size: 538 1028 263 601
Fragments arranged by size:
1028 601 538 263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 810 1112 1692 2430
Size: 810 302 580 738
Fragments arranged by size:
810 738 580 302
BsrBI CCG'CTC
Cuts at: 0 213 2430
Size: 213 2217
BsrGI T'GTAC_A
Cuts at: 0 2340 2430
Size: 2340 90
BstYI r'GATC_y
Cuts at: 0 435 492 1510 2430
Size: 435 57 1018 920
Fragments arranged by size:
1018 920 435 57
BtgI C'CryG_G
Cuts at: 0 1032 1933 2430
Size: 1032 901 497
Fragments arranged by size:
1032 901 497
BtsI GCAGTG_nn'
Cuts at: 0 459 630 2430
Size: 459 171 1800
Fragments arranged by size:
1800 459 171
DraI TTT'AAA
Cuts at: 0 383 1059 2430
Size: 383 676 1371
Fragments arranged by size:
1371 676 383
DrdI GACnn_nn'nnGTC
Cuts at: 0 2182 2430
Size: 2182 248
EaeI y'GGCC_r
Cuts at: 0 1530 2430
Size: 1530 900
EarI CTCTTCn'nnn_
Cuts at: 0 7 53 365 524 1085 2430
Size: 7 46 312 159 561 1345
Fragments arranged by size:
1345 561 312 159 46 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 789 2430
Size: 789 1641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 904 1118 1333 1379 1802 2430
Size: 149 755 214 215 46 423 628
Fragments arranged by size:
755 628 423 215 214 149 46
EcoO109I rG'GnC_Cy
Cuts at: 0 387 2020 2334 2430
Size: 387 1633 314 96
Fragments arranged by size:
1633 387 314 96
EcoRV GAT'ATC
Cuts at: 0 1230 2430
Size: 1230 1200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 721 2430
Size: 721 1709
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 1122 1154 1214 1246 1527 1559
Size: 202 32 888 32 60 32 281 32
Cuts at: 1559 2430
Size: 871
Fragments arranged by size:
888 871 281 202 60 32 32 32
32
HincII GTy'rAC
Cuts at: 0 887 2430
Size: 887 1543
HindIII A'AGCT_T
Cuts at: 0 161 593 2430
Size: 161 432 1837
Fragments arranged by size:
1837 432 161
HpaI GTT'AAC
Cuts at: 0 887 2430
Size: 887 1543
MfeI C'AATT_G
Cuts at: 0 392 2430
Size: 392 2038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 2430
Size: 629 1801
MslI CAynn'nnrTG
Cuts at: 0 2383 2430
Size: 2383 47
MspA1I CmG'CkG
Cuts at: 0 127 1642 2430
Size: 127 1515 788
Fragments arranged by size:
1515 788 127
NcoI C'CATG_G
Cuts at: 0 1032 2430
Size: 1032 1398
NspI r_CATG'y
Cuts at: 0 630 688 2382 2430
Size: 630 58 1694 48
Fragments arranged by size:
1694 630 58 48
PciI A'CATG_T
Cuts at: 0 2378 2430
Size: 2378 52
PflMI CCAn_nnn'nTGG
Cuts at: 0 1038 2430
Size: 1038 1392
PfoI T'CCnGG_A
Cuts at: 0 1618 2430
Size: 1618 812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 398 430 2430
Size: 398 32 2000
Fragments arranged by size:
2000 398 32
PpuMI rG'GwC_Cy
Cuts at: 0 2020 2334 2430
Size: 2020 314 96
Fragments arranged by size:
2020 314 96
PshAI GACnn'nnGTC
Cuts at: 0 1661 1856 2430
Size: 1661 195 574
Fragments arranged by size:
1661 574 195
PsiI TTA'TAA
Cuts at: 0 1325 2430
Size: 1325 1105
PvuII CAG'CTG
Cuts at: 0 127 1642 2430
Size: 127 1515 788
Fragments arranged by size:
1515 788 127
SfcI C'TryA_G
Cuts at: 0 1472 2430
Size: 1472 958
SmlI C'TyrA_G
Cuts at: 0 1159 1538 2430
Size: 1159 379 892
Fragments arranged by size:
1159 892 379
StuI AGG'CCT
Cuts at: 0 1578 2430
Size: 1578 852
StyI C'CwwG_G
Cuts at: 0 835 1032 1079 1236 1297 2390 2430
Size: 835 197 47 157 61 1093 40
Fragments arranged by size:
1093 835 197 157 61 47 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1673 2387 2428 2430
Size: 1673 714 41 2
Fragments arranged by size:
1673 714 41 2
TatI w'GTAC_w
Cuts at: 0 2340 2430
Size: 2340 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1422 1454 2430
Size: 1422 32 976
Fragments arranged by size:
1422 976 32
ZraI GAC'GTC
Cuts at: 0 949 2100 2430
Size: 949 1151 330
Fragments arranged by size:
1151 949 330
Enzymes that do cut:
AarI AatII AcuI AflIII AlfI AlwNI ApoI AvrII
BamHI BanII BbsI BbvCI BclI BlpI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrGI
BstYI BtgI BtsI DraI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII HpaI
MfeI MmeI MslI MspA1I NcoI NspI PciI PflMI
PfoI PpiI PpuMI PshAI PsiI PvuII SfcI SmlI
StuI StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BanI BbeI BcgI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmtI BplI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI
EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII KasI
KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI