Restriction map for
pF1KB4895
(Linear) (Six-base) MAPSORT of: KIBB4895.seq Check: 8625 from: 1 to: 1347
KIBB4895.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 587 1347
Size: 209 378 760
Fragments arranged by size:
760 378 209
AgeI A'CCGG_T
Cuts at: 0 982 1347
Size: 982 365
AhdI GACnn_n'nnGTC
Cuts at: 0 1332 1347
Size: 1332 15
AlwNI CAG_nnn'CTG
Cuts at: 0 1245 1347
Size: 1245 102
ApoI r'AATT_y
Cuts at: 0 1000 1083 1143 1347
Size: 1000 83 60 204
Fragments arranged by size:
1000 204 83 60
BanI G'GyrC_C
Cuts at: 0 700 1347
Size: 700 647
BanII G_rGCy'C
Cuts at: 0 1076 1347
Size: 1076 271
BclI T'GATC_A
Cuts at: 0 816 1347
Size: 816 531
BglII A'GATC_T
Cuts at: 0 545 1347
Size: 545 802
BlpI GC'TnA_GC
Cuts at: 0 7 66 1347
Size: 7 59 1281
Fragments arranged by size:
1281 59 7
Bme1580I G_kGCm'C
Cuts at: 0 533 866 1347
Size: 533 333 481
Fragments arranged by size:
533 481 333
BmtI G_CTAG'C
Cuts at: 0 1238 1347
Size: 1238 109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1305 1347
Size: 1305 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 779 1347
Size: 779 568
BsaWI w'CCGG_w
Cuts at: 0 982 1347
Size: 982 365
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 686 1347
Size: 686 661
BseYI C'CCAG_C
Cuts at: 0 676 1347
Size: 676 671
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1347
Size: 490 857
BsiHKAI G_wGCw'C
Cuts at: 0 13 856 1076 1347
Size: 13 843 220 271
Fragments arranged by size:
843 271 220 13
Bsp1286I G_dGCh'C
Cuts at: 0 13 533 856 866 1076 1347
Size: 13 520 323 10 210 271
Fragments arranged by size:
520 323 271 210 13 10
BspHI T'CATG_A
Cuts at: 0 1011 1347
Size: 1011 336
BsrFI r'CCGG_y
Cuts at: 0 982 1347
Size: 982 365
BsrGI T'GTAC_A
Cuts at: 0 870 1347
Size: 870 477
BstBI TT'CG_AA
Cuts at: 0 573 1347
Size: 573 774
BstEII G'GTnAC_C
Cuts at: 0 884 1347
Size: 884 463
BstYI r'GATC_y
Cuts at: 0 104 545 1347
Size: 104 441 802
Fragments arranged by size:
802 441 104
BtgI C'CryG_G
Cuts at: 0 230 1347
Size: 230 1117
BtsI GCAGTG_nn'
Cuts at: 0 424 1347
Size: 424 923
EaeI y'GGCC_r
Cuts at: 0 616 1347
Size: 616 731
EarI CTCTTCn'nnn_
Cuts at: 0 185 368 777 1081 1347
Size: 185 183 409 304 266
Fragments arranged by size:
409 304 266 185 183
EcoICRI GAG'CTC
Cuts at: 0 1074 1347
Size: 1074 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 587 1305 1347
Size: 209 378 718 42
Fragments arranged by size:
718 378 209 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 630 1347
Size: 630 717
EcoO109I rG'GnC_Cy
Cuts at: 0 48 622 1228 1347
Size: 48 574 606 119
Fragments arranged by size:
606 574 119 48
EcoRI G'AATT_C
Cuts at: 0 1143 1347
Size: 1143 204
HaeII r_GCGC'y
Cuts at: 0 1172 1347
Size: 1172 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1318 1347
Size: 823 32 463 29
Fragments arranged by size:
823 463 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1232 1347
Size: 380 852 115
Fragments arranged by size:
852 380 115
MslI CAynn'nnrTG
Cuts at: 0 607 1016 1064 1124 1347
Size: 607 409 48 60 223
Fragments arranged by size:
607 409 223 60 48
MspA1I CmG'CkG
Cuts at: 0 615 1245 1347
Size: 615 630 102
Fragments arranged by size:
630 615 102
NcoI C'CATG_G
Cuts at: 0 230 1347
Size: 230 1117
NheI G'CTAG_C
Cuts at: 0 1234 1347
Size: 1234 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 1188 1347
Size: 1188 159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 554 586 1347
Size: 554 32 761
Fragments arranged by size:
761 554 32
PpuMI rG'GwC_Cy
Cuts at: 0 622 1228 1347
Size: 622 606 119
Fragments arranged by size:
622 606 119
PsiI TTA'TAA
Cuts at: 0 917 1347
Size: 917 430
PstI C_TGCA'G
Cuts at: 0 1095 1313 1347
Size: 1095 218 34
Fragments arranged by size:
1095 218 34
PvuII CAG'CTG
Cuts at: 0 1245 1347
Size: 1245 102
SacI G_AGCT'C
Cuts at: 0 1076 1347
Size: 1076 271
SapI GCTCTTCn'nnn_
Cuts at: 0 1081 1347
Size: 1081 266
SfcI C'TryA_G
Cuts at: 0 363 1091 1148 1309 1347
Size: 363 728 57 161 38
Fragments arranged by size:
728 363 161 57 38
StuI AGG'CCT
Cuts at: 0 502 1347
Size: 502 845
StyI C'CwwG_G
Cuts at: 0 230 1347
Size: 230 1117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 1347
Size: 273 1074
TatI w'GTAC_w
Cuts at: 0 870 948 1347
Size: 870 78 399
Fragments arranged by size:
870 399 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 681 713 1347
Size: 681 32 634
Fragments arranged by size:
681 634 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1219 1246 1347
Size: 1219 27 101
Fragments arranged by size:
1219 101 27
Enzymes that do cut:
AcuI AgeI AhdI AlwNI ApoI BanI BanII BclI
BglII BlpI Bme1580I BmtI BpmI BsaI BsaWI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BsrFI BsrGI BstBI
BstEII BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I MmeI MslI MspA1I
NcoI NheI PflMI PpiI PpuMI PsiI PstI PvuII
SacI SapI SfcI StuI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BmgBI BmrI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI