Restriction map for
pF1KB4899
(Linear) (Six-base) MAPSORT of: KIBB4899.seq Check: 3405 from: 1 to: 1407
KIBB4899.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 0 1300 1407
Size: 1300 107
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 1407
Size: 243 1164
AfeI AGC'GCT
Cuts at: 0 1309 1407
Size: 1309 98
AflIII A'CryG_T
Cuts at: 0 1313 1407
Size: 1313 94
AlwNI CAG_nnn'CTG
Cuts at: 0 414 1407
Size: 414 993
ApoI r'AATT_y
Cuts at: 0 540 789 1407
Size: 540 249 618
Fragments arranged by size:
618 540 249
AvaI C'yCGr_G
Cuts at: 0 1360 1407
Size: 1360 47
BanI G'GyrC_C
Cuts at: 0 284 450 606 1407
Size: 284 166 156 801
Fragments arranged by size:
801 284 166 156
BanII G_rGCy'C
Cuts at: 0 865 1407
Size: 865 542
BbeI G_GCGC'C
Cuts at: 0 288 610 1407
Size: 288 322 797
Fragments arranged by size:
797 322 288
BclI T'GATC_A
Cuts at: 0 497 1407
Size: 497 910
BglI GCCn_nnn'nGGC
Cuts at: 0 43 1407
Size: 43 1364
BlpI GC'TnA_GC
Cuts at: 0 730 1249 1407
Size: 730 519 158
Fragments arranged by size:
730 519 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 82 1407
Size: 82 1325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 508 862 1129 1175 1321 1407
Size: 463 45 354 267 46 146 86
Fragments arranged by size:
463 354 267 146 86 46 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1407
Size: 52 1355
BsaAI yAC'GTr
Cuts at: 0 1314 1407
Size: 1314 93
BsaHI Gr'CG_yC
Cuts at: 0 285 607 814 1297 1385 1407
Size: 285 322 207 483 88 22
Fragments arranged by size:
483 322 285 207 88 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 814 844 1407
Size: 538 30 246 30 563
Fragments arranged by size:
563 538 246 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 1026 1407
Size: 865 161 381
Fragments arranged by size:
865 381 161
BseYI C'CCAG_C
Cuts at: 0 679 1347 1407
Size: 679 668 60
Fragments arranged by size:
679 668 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1354 1407
Size: 240 1114 53
Fragments arranged by size:
1114 240 53
BsiHKAI G_wGCw'C
Cuts at: 0 865 1407
Size: 865 542
BsmI GAATG_Cn'
Cuts at: 0 293 623 1407
Size: 293 330 784
Fragments arranged by size:
784 330 293
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1151 1407
Size: 1151 256
Bsp1286I G_dGCh'C
Cuts at: 0 865 1407
Size: 865 542
BspHI T'CATG_A
Cuts at: 0 180 1407
Size: 180 1227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1056 1407
Size: 1056 351
BsrFI r'CCGG_y
Cuts at: 0 1063 1202 1407
Size: 1063 139 205
Fragments arranged by size:
1063 205 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 153 1407
Size: 144 9 1254
Fragments arranged by size:
1254 144 9
BstBI TT'CG_AA
Cuts at: 0 941 1407
Size: 941 466
BtsI GCAGTG_nn'
Cuts at: 0 142 1407
Size: 142 1265
EaeI y'GGCC_r
Cuts at: 0 405 1061 1407
Size: 405 656 346
Fragments arranged by size:
656 405 346
EarI CTCTTCn'nnn_
Cuts at: 0 128 1407
Size: 128 1279
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 177 1407
Size: 177 1230
EcoICRI GAG'CTC
Cuts at: 0 863 1407
Size: 863 544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 463 508 862 1129 1175 1321 1407
Size: 243 220 45 354 267 46 146 86
Fragments arranged by size:
354 267 243 220 146 86 46 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 1407
Size: 427 980
EcoO109I rG'GnC_Cy
Cuts at: 0 101 1407
Size: 101 1306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 453 1407
Size: 453 954
HaeII r_GCGC'y
Cuts at: 0 75 288 610 1311 1407
Size: 75 213 322 701 96
Fragments arranged by size:
701 322 213 96 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 814 846 1102 1134 1407
Size: 510 32 272 32 256 32 273
Fragments arranged by size:
510 273 272 256 32 32 32
HincII GTy'rAC
Cuts at: 0 212 1407
Size: 212 1195
HpaI GTT'AAC
Cuts at: 0 212 1407
Size: 212 1195
KasI G'GCGC_C
Cuts at: 0 284 606 1407
Size: 284 322 801
Fragments arranged by size:
801 322 284
MscI TGG'CCA
Cuts at: 0 407 1407
Size: 407 1000
MspA1I CmG'CkG
Cuts at: 0 282 1054 1092 1148 1407
Size: 282 772 38 56 259
Fragments arranged by size:
772 282 259 56 38
NaeI GCC'GGC
Cuts at: 0 1065 1407
Size: 1065 342
NarI GG'CG_CC
Cuts at: 0 285 607 1407
Size: 285 322 800
Fragments arranged by size:
800 322 285
NgoMIV G'CCGG_C
Cuts at: 0 1063 1407
Size: 1063 344
NspI r_CATG'y
Cuts at: 0 663 1407
Size: 663 744
PfoI T'CCnGG_A
Cuts at: 0 650 883 1106 1407
Size: 650 233 223 301
Fragments arranged by size:
650 301 233 223
PpuMI rG'GwC_Cy
Cuts at: 0 101 1407
Size: 101 1306
PstI C_TGCA'G
Cuts at: 0 389 955 1053 1407
Size: 389 566 98 354
Fragments arranged by size:
566 389 354 98
PvuII CAG'CTG
Cuts at: 0 1054 1092 1148 1407
Size: 1054 38 56 259
Fragments arranged by size:
1054 259 56 38
SacI G_AGCT'C
Cuts at: 0 865 1407
Size: 865 542
SanDI GG'GwC_CC
Cuts at: 0 101 1407
Size: 101 1306
SapI GCTCTTCn'nnn_
Cuts at: 0 128 1407
Size: 128 1279
SexAI A'CCwGG_T
Cuts at: 0 29 1407
Size: 29 1378
SfcI C'TryA_G
Cuts at: 0 385 951 1049 1407
Size: 385 566 98 358
Fragments arranged by size:
566 385 358 98
SfoI GGC'GCC
Cuts at: 0 286 608 1407
Size: 286 322 799
Fragments arranged by size:
799 322 286
SgrAI Cr'CCGG_yG
Cuts at: 0 1202 1407
Size: 1202 205
SmlI C'TyrA_G
Cuts at: 0 67 1407
Size: 67 1340
StyI C'CwwG_G
Cuts at: 0 423 1407
Size: 423 984
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 693 1407
Size: 454 239 714
Fragments arranged by size:
714 454 239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 543 570 575 646 678 1407
Size: 538 5 27 5 71 32 729
Fragments arranged by size:
729 538 71 32 27 5 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 305 1407
Size: 305 1102
XmnI GAAnn'nnTTC
Cuts at: 0 7 437 1407
Size: 7 430 970
Fragments arranged by size:
970 430 7
ZraI GAC'GTC
Cuts at: 0 1298 1407
Size: 1298 109
Enzymes that do cut:
AatII AcuI AfeI AflIII AlwNI ApoI AvaI BanI
BanII BbeI BclI BglI BlpI BplI BpmI BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrFI BstAPI BstBI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HpaI KasI MscI MspA1I NaeI
NarI NgoMIV NspI PfoI PpuMI PstI PvuII SacI
SanDI SapI SexAI SfcI SfoI SgrAI SmlI StyI
TaqII TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglII
Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaWI
BsiEI BsiWI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI
FspI FspAI HindIII KpnI MfeI MluI MmeI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XhoI XmaI