Restriction map for
pF1KB4907
(Linear) (Six-base) MAPSORT of: KIBB4907.seq Check: 3872 from: 1 to: 1206
KIBB4907.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AatII G_ACGT'C
Cuts at: 0 274 1206
Size: 274 932
AccI GT'mk_AC
Cuts at: 0 587 1206
Size: 587 619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1021 1206
Size: 386 635 185
Fragments arranged by size:
635 386 185
AleI CACnn'nnGTG
Cuts at: 0 953 1206
Size: 953 253
AlwNI CAG_nnn'CTG
Cuts at: 0 316 1206
Size: 316 890
ApaI G_GGCC'C
Cuts at: 0 440 1206
Size: 440 766
ApoI r'AATT_y
Cuts at: 0 519 1206
Size: 519 687
AvaI C'yCGr_G
Cuts at: 0 683 1206
Size: 683 523
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 380 413 799 832 1029 1062 1206
Size: 380 33 386 33 197 33 144
Fragments arranged by size:
386 380 197 144 33 33 33
BanI G'GyrC_C
Cuts at: 0 576 1206
Size: 576 630
BanII G_rGCy'C
Cuts at: 0 194 440 1206
Size: 194 246 766
Fragments arranged by size:
766 246 194
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 1206
Size: 347 859
BciVI GTATCCnnnnn_n'
Cuts at: 0 955 1206
Size: 955 251
Bme1580I G_kGCm'C
Cuts at: 0 440 1206
Size: 440 766
BmrI ACTGGGnnnn_n'
Cuts at: 0 292 814 1206
Size: 292 522 392
Fragments arranged by size:
522 392 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 915 1206
Size: 416 499 291
Fragments arranged by size:
499 416 291
BsaI GGTCTCn'nnnn_
Cuts at: 0 128 1206
Size: 128 1078
BsaHI Gr'CG_yC
Cuts at: 0 271 903 1206
Size: 271 632 303
Fragments arranged by size:
632 303 271
BsaWI w'CCGG_w
Cuts at: 0 1010 1206
Size: 1010 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 1206
Size: 190 30 986
Fragments arranged by size:
986 190 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 312 1206
Size: 312 894
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1044 1165 1206
Size: 1044 121 41
Fragments arranged by size:
1044 121 41
BsiHKAI G_wGCw'C
Cuts at: 0 898 1206
Size: 898 308
BsmBI CGTCTCn'nnnn_
Cuts at: 0 903 1206
Size: 903 303
Bsp1286I G_dGCh'C
Cuts at: 0 194 440 898 1206
Size: 194 246 458 308
Fragments arranged by size:
458 308 246 194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 740 1206
Size: 740 466
BsrFI r'CCGG_y
Cuts at: 0 78 1206
Size: 78 1128
BsrGI T'GTAC_A
Cuts at: 0 980 1206
Size: 980 226
BstAPI GCAn_nnn'nTGC
Cuts at: 0 480 1206
Size: 480 726
BstEII G'GTnAC_C
Cuts at: 0 602 1206
Size: 602 604
BstYI r'GATC_y
Cuts at: 0 769 1206
Size: 769 437
BtgI C'CryG_G
Cuts at: 0 560 660 1206
Size: 560 100 546
Fragments arranged by size:
560 546 100
EarI CTCTTCn'nnn_
Cuts at: 0 10 351 1206
Size: 10 341 855
Fragments arranged by size:
855 341 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 416 915 1021 1206
Size: 386 30 499 106 185
Fragments arranged by size:
499 386 185 106 30
EcoO109I rG'GnC_Cy
Cuts at: 0 436 437 1206
Size: 436 1 769
Fragments arranged by size:
769 436 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 1206
Size: 193 32 981
Fragments arranged by size:
981 193 32
HincII GTy'rAC
Cuts at: 0 419 1206
Size: 419 787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 1206
Size: 444 762
MslI CAynn'nnrTG
Cuts at: 0 559 953 1131 1206
Size: 559 394 178 75
Fragments arranged by size:
559 394 178 75
MspA1I CmG'CkG
Cuts at: 0 595 1206
Size: 595 611
NaeI GCC'GGC
Cuts at: 0 80 1206
Size: 80 1126
NcoI C'CATG_G
Cuts at: 0 660 1206
Size: 660 546
NdeI CA'TA_TG
Cuts at: 0 11 1196 1206
Size: 11 1185 10
Fragments arranged by size:
1185 11 10
NgoMIV G'CCGG_C
Cuts at: 0 78 1206
Size: 78 1128
PspOMI G'GGCC_C
Cuts at: 0 436 1206
Size: 436 770
PvuII CAG'CTG
Cuts at: 0 595 1206
Size: 595 611
SfcI C'TryA_G
Cuts at: 0 212 1206
Size: 212 994
StuI AGG'CCT
Cuts at: 0 689 1206
Size: 689 517
StyI C'CwwG_G
Cuts at: 0 660 1206
Size: 660 546
TatI w'GTAC_w
Cuts at: 0 798 980 1206
Size: 798 182 226
Fragments arranged by size:
798 226 182
XmnI GAAnn'nnTTC
Cuts at: 0 158 1126 1206
Size: 158 968 80
Fragments arranged by size:
968 158 80
ZraI GAC'GTC
Cuts at: 0 272 1206
Size: 272 934
Enzymes that do cut:
AatII AccI AcuI AleI AlwNI ApaI ApoI AvaI
BaeI BanI BanII BbsI BciVI Bme1580I BmrI BpmI
BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BsrGI BstAPI BstEII BstYI BtgI
EarI Eco57MI EcoO109I Hin4I HincII MmeI MslI MspA1I
NaeI NcoI NdeI NgoMIV PspOMI PvuII SfcI StuI
StyI TatI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BseYI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII
BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI