Restriction map for
pF1KB4919
(Linear) (Six-base) MAPSORT of: KIBB4919.seq Check: 1583 from: 1 to: 5499
KIBB4919.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 753 1499 1563 5499
Size: 753 746 64 3936
Fragments arranged by size:
3936 753 746 64
AccI GT'mk_AC
Cuts at: 0 5280 5499
Size: 5280 219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 479 644 1401 1534 2840 3052 4019
Size: 49 430 165 757 133 1306 212 967
Cuts at: 4019 4232 4695 4776 5499
Size: 213 463 81 723
Fragments arranged by size:
1306 967 757 723 463 430 213 212
165 133 81 49
AflIII A'CryG_T
Cuts at: 0 5256 5499
Size: 5256 243
AhdI GACnn_n'nnGTC
Cuts at: 0 516 5499
Size: 516 4983
AleI CACnn'nnGTG
Cuts at: 0 319 5499
Size: 319 5180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1511 2497 2803 5499
Size: 1511 986 306 2696
Fragments arranged by size:
2696 1511 986 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2144 2145 2176 2177 5499
Size: 2144 1 31 1 3322
Fragments arranged by size:
3322 2144 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 2343 3769 5499
Size: 2343 1426 1730
Fragments arranged by size:
2343 1730 1426
ApaLI G'TGCA_C
Cuts at: 0 5252 5499
Size: 5252 247
ApoI r'AATT_y
Cuts at: 0 910 3269 3783 5499
Size: 910 2359 514 1716
Fragments arranged by size:
2359 1716 910 514
AvaI C'yCGr_G
Cuts at: 0 1602 2801 5197 5499
Size: 1602 1199 2396 302
Fragments arranged by size:
2396 1602 1199 302
AvrII C'CTAG_G
Cuts at: 0 525 4393 5499
Size: 525 3868 1106
Fragments arranged by size:
3868 1106 525
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2168 2201 3600 3633 5499
Size: 2168 33 1399 33 1866
Fragments arranged by size:
2168 1866 1399 33 33
BamHI G'GATC_C
Cuts at: 0 186 2171 3457 4859 5499
Size: 186 1985 1286 1402 640
Fragments arranged by size:
1985 1402 1286 640 186
BanI G'GyrC_C
Cuts at: 0 195 1517 2805 4012 4845 5499
Size: 195 1322 1288 1207 833 654
Fragments arranged by size:
1322 1288 1207 833 654 195
BanII G_rGCy'C
Cuts at: 0 714 1603 2510 2905 4833 5231 5499
Size: 714 889 907 395 1928 398 268
Fragments arranged by size:
1928 907 889 714 398 395 268
BbeI G_GCGC'C
Cuts at: 0 4016 5499
Size: 4016 1483
BbsI GAAGACnn'nnnn_
Cuts at: 0 2747 4782 5102 5499
Size: 2747 2035 320 397
Fragments arranged by size:
2747 2035 397 320
BbvCI CC'TCA_GC
Cuts at: 0 3860 3974 5307 5499
Size: 3860 114 1333 192
Fragments arranged by size:
3860 1333 192 114
BciVI GTATCCnnnnn_n'
Cuts at: 0 1799 5499
Size: 1799 3700
BclI T'GATC_A
Cuts at: 0 3840 4064 5499
Size: 3840 224 1435
Fragments arranged by size:
3840 1435 224
BfrBI ATG'CAT
Cuts at: 0 3291 5499
Size: 3291 2208
BglII A'GATC_T
Cuts at: 0 4401 5499
Size: 4401 1098
BlpI GC'TnA_GC
Cuts at: 0 1597 1820 1865 5499
Size: 1597 223 45 3634
Fragments arranged by size:
3634 1597 223 45
Bme1580I G_kGCm'C
Cuts at: 0 2808 4850 5256 5499
Size: 2808 2042 406 243
Fragments arranged by size:
2808 2042 406 243
BmgBI CAC'GTC
Cuts at: 0 3541 5499
Size: 3541 1958
BmrI ACTGGGnnnn_n'
Cuts at: 0 688 3071 4112 5499
Size: 688 2383 1041 1387
Fragments arranged by size:
2383 1387 1041 688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 4275 4989 5499
Size: 1026 3249 714 510
Fragments arranged by size:
3249 1026 714 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1074 1504 2474 4286 4740 5499
Size: 1074 430 970 1812 454 759
Fragments arranged by size:
1812 1074 970 759 454 430
Bpu10I CC'TnA_GC
Cuts at: 0 1145 3860 3974 5285 5307 5499
Size: 1145 2715 114 1311 22 192
Fragments arranged by size:
2715 1311 1145 192 114 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 2714 4269 5499
Size: 171 2543 1555 1230
Fragments arranged by size:
2543 1555 1230 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 928 4535 4718 5499
Size: 928 3607 183 781
Fragments arranged by size:
3607 928 781 183
BsaAI yAC'GTr
Cuts at: 0 2184 5499
Size: 2184 3315
BsaHI Gr'CG_yC
Cuts at: 0 4013 5499
Size: 4013 1486
BsaWI w'CCGG_w
Cuts at: 0 1505 2794 5168 5499
Size: 1505 1289 2374 331
Fragments arranged by size:
2374 1505 1289 331
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 859 999 1535 2587 2697 2712 3981
Size: 499 360 140 536 1052 110 15 1269
Cuts at: 3981 4246 4251 4957 5049 5054 5499
Size: 265 5 706 92 5 445
Fragments arranged by size:
1269 1052 706 536 499 445 360 265
140 110 92 15 5 5
BseYI C'CCAG_C
Cuts at: 0 944 1060 1278 1622 2128 2343 2639 2776
Size: 944 116 218 344 506 215 296 137
Cuts at: 2776 3004 5499
Size: 228 2495
Fragments arranged by size:
2495 944 506 344 296 228 218 215
137 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 787 1121 1597 5242 5499
Size: 749 38 334 476 3645 257
Fragments arranged by size:
3645 749 476 334 257 38
BsiHKAI G_wGCw'C
Cuts at: 0 1007 1490 1603 1949 2510 5089 5256 5499
Size: 1007 483 113 346 561 2579 167 243
Fragments arranged by size:
2579 1007 561 483 346 243 167 113
BsmI GAATG_Cn'
Cuts at: 0 1167 1773 2486 5499
Size: 1167 606 713 3013
Fragments arranged by size:
3013 1167 713 606
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2872 5499
Size: 2872 2627
Bsp1286I G_dGCh'C
Cuts at: 0 714 1007 1490 1603 1949 2510 2808 2905
Size: 714 293 483 113 346 561 298 97
Cuts at: 2905 4833 4850 5089 5231 5256 5499
Size: 1928 17 239 142 25 243
Fragments arranged by size:
1928 714 561 483 346 298 293 243
239 142 113 97 25 17
BspEI T'CCGG_A
Cuts at: 0 5168 5499
Size: 5168 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 753 1499 1563 1971 2085 5499
Size: 753 746 64 408 114 3414
Fragments arranged by size:
3414 753 746 408 114 64
BsrBI CCG'CTC
Cuts at: 0 5118 5499
Size: 5118 381
BsrDI GCAATG_nn'
Cuts at: 0 199 2330 4915 5156 5499
Size: 199 2131 2585 241 343
Fragments arranged by size:
2585 2131 343 241 199
BsrFI r'CCGG_y
Cuts at: 0 3304 5499
Size: 3304 2195
BsrGI T'GTAC_A
Cuts at: 0 2207 5146 5499
Size: 2207 2939 353
Fragments arranged by size:
2939 2207 353
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4824 5499
Size: 4824 675
BstEII G'GTnAC_C
Cuts at: 0 2295 5499
Size: 2295 3204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2502 5499
Size: 2502 2997
BstYI r'GATC_y
Cuts at: 0 186 2171 3457 4401 4859 5469 5499
Size: 186 1985 1286 944 458 610 30
Fragments arranged by size:
1985 1286 944 610 458 186 30
Bsu36I CC'TnA_GG
Cuts at: 0 1011 4697 4769 5499
Size: 1011 3686 72 730
Fragments arranged by size:
3686 1011 730 72
BtgI C'CryG_G
Cuts at: 0 191 389 953 1361 1521 4023 4631 4841
Size: 191 198 564 408 160 2502 608 210
Cuts at: 4841 5177 5499
Size: 336 322
Fragments arranged by size:
2502 608 564 408 336 322 210 198
191 160
BtsI GCAGTG_nn'
Cuts at: 0 1609 3260 5499
Size: 1609 1651 2239
Fragments arranged by size:
2239 1651 1609
ClaI AT'CG_AT
Cuts at: 0 2873 5499
Size: 2873 2626
DraI TTT'AAA
Cuts at: 0 161 5499
Size: 161 5338
DraIII CAC_nnn'GTG
Cuts at: 0 4120 5499
Size: 4120 1379
DrdI GACnn_nn'nnGTC
Cuts at: 0 76 5499
Size: 76 5423
EaeI y'GGCC_r
Cuts at: 0 2456 3543 5499
Size: 2456 1087 1956
Fragments arranged by size:
2456 1956 1087
EarI CTCTTCn'nnn_
Cuts at: 0 256 272 1553 1873 2515 2939 3035 3242
Size: 256 16 1281 320 642 424 96 207
Cuts at: 3242 4446 4533 4551 4680 5177 5499
Size: 1204 87 18 129 497 322
Fragments arranged by size:
1281 1204 642 497 424 322 320 256
207 129 96 87 18 16
EcoICRI GAG'CTC
Cuts at: 0 1601 2508 5499
Size: 1601 907 2991
Fragments arranged by size:
2991 1601 907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 479 644 1074 1401 1504 1534 2474
Size: 49 430 165 430 327 103 30 940
Cuts at: 2474 2840 3052 4019 4232 4286 4695 4740 4776
Size: 366 212 967 213 54 409 45 36
Cuts at: 4776 5499
Size: 723
Fragments arranged by size:
967 940 723 430 430 409 366 327
213 212 165 103 54 49 45 36
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4266 5288 5499
Size: 4266 1022 211
Fragments arranged by size:
4266 1022 211
EcoO109I rG'GnC_Cy
Cuts at: 0 1560 1582 2616 4669 4774 4980 5017 5499
Size: 1560 22 1034 2053 105 206 37 482
Fragments arranged by size:
2053 1560 1034 482 206 105 37 22
EcoRV GAT'ATC
Cuts at: 0 2871 4527 5499
Size: 2871 1656 972
Fragments arranged by size:
2871 1656 972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1410 5499
Size: 1410 4089
HaeII r_GCGC'y
Cuts at: 0 4016 4591 5499
Size: 4016 575 908
Fragments arranged by size:
4016 908 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 1254 1286 2101 2133 2869 2901
Size: 502 32 720 32 815 32 736 32
Cuts at: 2901 3442 3474 4820 4852 5461 5493 5499
Size: 541 32 1346 32 609 32 6
Fragments arranged by size:
1346 815 736 720 609 541 502 32
32 32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 0 2075 5499
Size: 2075 3424
HindIII A'AGCT_T
Cuts at: 0 1129 1409 3310 5499
Size: 1129 280 1901 2189
Fragments arranged by size:
2189 1901 1129 280
KasI G'GCGC_C
Cuts at: 0 4012 5499
Size: 4012 1487
MfeI C'AATT_G
Cuts at: 0 4658 5499
Size: 4658 841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1701 5399 5499
Size: 64 1637 3698 100
Fragments arranged by size:
3698 1637 100 64
MscI TGG'CCA
Cuts at: 0 2458 5499
Size: 2458 3041
MslI CAynn'nnrTG
Cuts at: 0 289 319 1493 1639 2896 3043 3626 5333
Size: 289 30 1174 146 1257 147 583 1707
Cuts at: 5333 5499
Size: 166
Fragments arranged by size:
1707 1257 1174 583 289 166 147 146
30
MspA1I CmG'CkG
Cuts at: 0 955 1282 1482 1596 2643 2940 3008 5499
Size: 955 327 200 114 1047 297 68 2491
Fragments arranged by size:
2491 1047 955 327 297 200 114 68
NarI GG'CG_CC
Cuts at: 0 4013 5499
Size: 4013 1486
NcoI C'CATG_G
Cuts at: 0 389 1521 4631 4841 5499
Size: 389 1132 3110 210 658
Fragments arranged by size:
3110 1132 658 389 210
NdeI CA'TA_TG
Cuts at: 0 2257 3695 5499
Size: 2257 1438 1804
Fragments arranged by size:
2257 1804 1438
NsiI A_TGCA'T
Cuts at: 0 3293 5499
Size: 3293 2206
NspI r_CATG'y
Cuts at: 0 222 1498 1939 2356 2982 3600 3825 4242
Size: 222 1276 441 417 626 618 225 417
Cuts at: 4242 5260 5499
Size: 1018 239
Fragments arranged by size:
1276 1018 626 618 441 417 417 239
225 222
PasI CC'CwG_GG
Cuts at: 0 1440 1564 2463 4047 4268 5244 5499
Size: 1440 124 899 1584 221 976 255
Fragments arranged by size:
1584 1440 976 899 255 221 124
PciI A'CATG_T
Cuts at: 0 5256 5499
Size: 5256 243
PflMI CCAn_nnn'nTGG
Cuts at: 0 2194 2343 2677 5499
Size: 2194 149 334 2822
Fragments arranged by size:
2822 2194 334 149
PfoI T'CCnGG_A
Cuts at: 0 543 4302 5499
Size: 543 3759 1197
Fragments arranged by size:
3759 1197 543
PpuMI rG'GwC_Cy
Cuts at: 0 1560 1582 2616 4774 4980 5017 5499
Size: 1560 22 1034 2158 206 37 482
Fragments arranged by size:
2158 1560 1034 482 206 37 22
PshAI GACnn'nnGTC
Cuts at: 0 440 2293 5499
Size: 440 1853 3206
Fragments arranged by size:
3206 1853 440
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3 35 5499
Size: 3 32 5464
Fragments arranged by size:
5464 32 3
PstI C_TGCA'G
Cuts at: 0 301 359 458 3267 5499
Size: 301 58 99 2809 2232
Fragments arranged by size:
2809 2232 301 99 58
PvuII CAG'CTG
Cuts at: 0 1482 2643 2940 5499
Size: 1482 1161 297 2559
Fragments arranged by size:
2559 1482 1161 297
RsrII CG'GwC_CG
Cuts at: 0 1508 5499
Size: 1508 3991
SacI G_AGCT'C
Cuts at: 0 1603 2510 5499
Size: 1603 907 2989
Fragments arranged by size:
2989 1603 907
SacII CC_GC'GG
Cuts at: 0 956 5499
Size: 956 4543
SapI GCTCTTCn'nnn_
Cuts at: 0 256 1873 2515 3242 5499
Size: 256 1617 642 727 2257
Fragments arranged by size:
2257 1617 727 642 256
SfcI C'TryA_G
Cuts at: 0 132 297 355 454 1793 3263 5499
Size: 132 165 58 99 1339 1470 2236
Fragments arranged by size:
2236 1470 1339 165 132 99 58
SfoI GGC'GCC
Cuts at: 0 4014 5499
Size: 4014 1485
SmaI CCC'GGG
Cuts at: 0 2803 5499
Size: 2803 2696
SmlI C'TyrA_G
Cuts at: 0 150 2729 4284 5499
Size: 150 2579 1555 1215
Fragments arranged by size:
2579 1555 1215 150
SpeI A'CTAG_T
Cuts at: 0 3396 5499
Size: 3396 2103
SphI G_CATG'C
Cuts at: 0 1498 2356 2982 3600 4242 5499
Size: 1498 858 626 618 642 1257
Fragments arranged by size:
1498 1257 858 642 626 618
SspI AAT'ATT
Cuts at: 0 178 3491 5499
Size: 178 3313 2008
Fragments arranged by size:
3313 2008 178
StuI AGG'CCT
Cuts at: 0 1046 3297 5499
Size: 1046 2251 2202
Fragments arranged by size:
2251 2202 1046
StyI C'CwwG_G
Cuts at: 0 389 525 1521 4393 4498 4631 4841 4976
Size: 389 136 996 2872 105 133 210 135
Cuts at: 4976 5499
Size: 523
Fragments arranged by size:
2872 996 523 389 210 136 135 133
105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 856 984 1292 2042 4131 4425 4926 5329
Size: 856 128 308 750 2089 294 501 403
Cuts at: 5329 5499
Size: 170
Fragments arranged by size:
2089 856 750 501 403 308 294 170
128
TatI w'GTAC_w
Cuts at: 0 2207 3554 5146 5499
Size: 2207 1347 1592 353
Fragments arranged by size:
2207 1592 1347 353
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2173 2205 2552 2584 4219 4251 5499
Size: 2173 32 347 32 1635 32 1248
Fragments arranged by size:
2173 1635 1248 347 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4786 5499
Size: 4786 713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 457 1447 3012 3228 5499
Size: 457 990 1565 216 2271
Fragments arranged by size:
2271 1565 990 457 216
XmaI C'CCGG_G
Cuts at: 0 2801 5499
Size: 2801 2698
XmnI GAAnn'nnTTC
Cuts at: 0 911 2484 5499
Size: 911 1573 3015
Fragments arranged by size:
3015 1573 911
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
ClaI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MscI MslI MspA1I NarI NcoI
NdeI NsiI NspI PasI PciI PflMI PfoI PpuMI
PshAI PsrI PstI PvuII RsrII SacI SacII SapI
SfcI SfoI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI ApaI AscI
AseI AsiSI BcgI BglI BmtI BsaBI BsaXI BsiEI
BsiWI BspHI BssHII BssSI BstBI BstZ17I BtgZI CspCI
EagI EciI EcoRI FseI FspI FspAI HpaI KpnI
MluI NaeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PsiI PspOMI PspXI PvuI SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SrfI
SwaI XbaI XhoI ZraI