(Linear) (Six-base) MAPSORT of: KIBB4944.seq  Check: 8000  from: 1  to: 1536



KIBB4944.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1354    1536 
   Size:       1354     182
 
AleI CACnn'nnGTG

Cuts at:      0    1457    1536 
   Size:       1457      79
 
ApoI r'AATT_y

Cuts at:      0     787    1536 
   Size:        787     749
 
AseI AT'TA_AT

Cuts at:      0     401    1418    1536 
   Size:        401    1017     118

  Fragments arranged by size: 

                1017     401     118
 
AvaI C'yCGr_G

Cuts at:      0    1343    1536 
   Size:       1343     193
 
BanII G_rGCy'C

Cuts at:      0     828    1536 
   Size:        828     708
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     220     532     721    1536 
   Size:        220     312     189     815

  Fragments arranged by size: 

                 815     312     220     189
 
BbvCI CC'TCA_GC

Cuts at:      0    1482    1536 
   Size:       1482      54
 
Bpu10I CC'TnA_GC

Cuts at:      0    1482    1536 
   Size:       1482      54
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1234    1536 
   Size:       1234     302
 
BsaHI Gr'CG_yC

Cuts at:      0    1286    1536 
   Size:       1286     250
 
BsaWI w'CCGG_w

Cuts at:      0    1382    1536 
   Size:       1382     154
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     633     663    1536 
   Size:        633      30     873

  Fragments arranged by size: 

                 873     633      30
 
BseYI C'CCAG_C

Cuts at:      0     844    1536 
   Size:        844     692
 
BsiEI CG_ry'CG

Cuts at:      0      36    1536 
   Size:         36    1500
 
BsiHKAI G_wGCw'C

Cuts at:      0     728     757    1094    1536 
   Size:        728      29     337     442

  Fragments arranged by size: 

                 728     442     337      29
 
BsmI GAATG_Cn'

Cuts at:      0     448    1433    1536 
   Size:        448     985     103

  Fragments arranged by size: 

                 985     448     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     728     757     828    1094    1536 
   Size:        728      29      71     266     442

  Fragments arranged by size: 

                 728     442     266      71      29
 
BspEI T'CCGG_A

Cuts at:      0    1382    1536 
   Size:       1382     154
 
BspHI T'CATG_A

Cuts at:      0     438     971    1536 
   Size:        438     533     565

  Fragments arranged by size: 

                 565     533     438
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     683    1536 
   Size:        683     853
 
BsrDI GCAATG_nn'

Cuts at:      0     714    1536 
   Size:        714     822
 
BsrGI T'GTAC_A

Cuts at:      0     579     857    1536 
   Size:        579     278     679

  Fragments arranged by size: 

                 679     579     278
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     844    1536 
   Size:        844     692
 
BstYI r'GATC_y

Cuts at:      0    1379    1536 
   Size:       1379     157
 
Bsu36I CC'TnA_GG

Cuts at:      0     202    1536 
   Size:        202    1334
 
DraI TTT'AAA

Cuts at:      0     125     395    1536 
   Size:        125     270    1141

  Fragments arranged by size: 

                1141     270     125
 
EarI CTCTTCn'nnn_

Cuts at:      0     152     859    1536 
   Size:        152     707     677

  Fragments arranged by size: 

                 707     677     152
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1354    1536 
   Size:       1354     182
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1254    1536 
   Size:       1254     282
 
FspI TGC'GCA

Cuts at:      0    1502    1536 
   Size:       1502      34
 
HaeII r_GCGC'y

Cuts at:      0      15    1536 
   Size:         15    1521
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     390     422    1536 
   Size:        390      32    1114

  Fragments arranged by size: 

                1114     390      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     279    1536 
   Size:         88     191    1257

  Fragments arranged by size: 

                1257     191      88
 
MslI CAynn'nnrTG

Cuts at:      0    1075    1457    1493    1536 
   Size:       1075     382      36      43

  Fragments arranged by size: 

                1075     382      43      36
 
NdeI CA'TA_TG

Cuts at:      0    1099    1536 
   Size:       1099     437
 
NspI r_CATG'y

Cuts at:      0    1498    1536 
   Size:       1498      38
 
PfoI T'CCnGG_A

Cuts at:      0       4    1536 
   Size:          4    1532
 
PstI C_TGCA'G

Cuts at:      0     780    1536 
   Size:        780     756
 
SapI GCTCTTCn'nnn_

Cuts at:      0     859    1536 
   Size:        859     677
 
SfcI C'TryA_G

Cuts at:      0     765     776    1148    1536 
   Size:        765      11     372     388

  Fragments arranged by size: 

                 765     388     372      11
 
SmaI CCC'GGG

Cuts at:      0    1345    1536 
   Size:       1345     191
 
SmlI C'TyrA_G

Cuts at:      0    1249    1536 
   Size:       1249     287
 
SspI AAT'ATT

Cuts at:      0     496    1536 
   Size:        496    1040
 
StyI C'CwwG_G

Cuts at:      0     719    1515    1536 
   Size:        719     796      21

  Fragments arranged by size: 

                 796     719      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     325    1427    1536 
   Size:        325    1102     109

  Fragments arranged by size: 

                1102     325     109
 
TatI w'GTAC_w

Cuts at:      0     579     857    1536 
   Size:        579     278     679

  Fragments arranged by size: 

                 679     579     278
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     867     899    1536 
   Size:        867      32     637

  Fragments arranged by size: 

                 867     637      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      37    1536 
   Size:         37    1499
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1007    1194    1536 
   Size:       1007     187     342

  Fragments arranged by size: 

                1007     342     187
 
XmaI C'CCGG_G

Cuts at:      0    1343    1536 
   Size:       1343     193
 

 Enzymes that do cut:

     AcuI     AleI     ApoI     AseI     AvaI    BanII     BbsI    BbvCI
   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI    BstXI
    BstYI   Bsu36I     DraI     EarI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I     MmeI     MslI     NdeI     NspI     PfoI     PstI     SapI
     SfcI     SmaI     SmlI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BseRI     BsgI
    BsiWI    BsmBI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     XbaI     XhoI     XmnI
     ZraI