Restriction map for
pF1KB4959
(Linear) (Six-base) MAPSORT of: KIBB4959.seq Check: 3095 from: 1 to: 1704
KIBB4959.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 0 1518 1704
Size: 1518 186
AccI GT'mk_AC
Cuts at: 0 1373 1704
Size: 1373 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 808 1009 1704
Size: 322 486 201 695
Fragments arranged by size:
695 486 322 201
AfeI AGC'GCT
Cuts at: 0 706 1704
Size: 706 998
AlwNI CAG_nnn'CTG
Cuts at: 0 536 692 1173 1224 1704
Size: 536 156 481 51 480
Fragments arranged by size:
536 481 480 156 51
ApaLI G'TGCA_C
Cuts at: 0 656 1180 1704
Size: 656 524 524
Fragments arranged by size:
656 524 524
ApoI r'AATT_y
Cuts at: 0 326 1704
Size: 326 1378
BanI G'GyrC_C
Cuts at: 0 206 1704
Size: 206 1498
BanII G_rGCy'C
Cuts at: 0 247 1704
Size: 247 1457
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 584 1312 1704
Size: 139 445 728 392
Fragments arranged by size:
728 445 392 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 637 671 789 823 1105 1139 1704
Size: 637 34 118 34 282 34 565
Fragments arranged by size:
637 565 282 118 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1674 1704
Size: 1674 30
BfrBI ATG'CAT
Cuts at: 0 1531 1704
Size: 1531 173
BglI GCCn_nnn'nGGC
Cuts at: 0 754 1020 1704
Size: 754 266 684
Fragments arranged by size:
754 684 266
BglII A'GATC_T
Cuts at: 0 341 1704
Size: 341 1363
BlpI GC'TnA_GC
Cuts at: 0 241 1153 1704
Size: 241 912 551
Fragments arranged by size:
912 551 241
Bme1580I G_kGCm'C
Cuts at: 0 209 660 1184 1704
Size: 209 451 524 520
Fragments arranged by size:
524 520 451 209
BmgBI CAC'GTC
Cuts at: 0 777 1704
Size: 777 927
BmrI ACTGGGnnnn_n'
Cuts at: 0 1038 1597 1704
Size: 1038 559 107
Fragments arranged by size:
1038 559 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 777 1704
Size: 777 927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 1111 1162 1246 1595 1704
Size: 1054 57 51 84 349 109
Fragments arranged by size:
1054 349 109 84 57 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 1704
Size: 492 1212
BsaI GGTCTCn'nnnn_
Cuts at: 0 482 1222 1704
Size: 482 740 482
Fragments arranged by size:
740 482 482
BsaAI yAC'GTr
Cuts at: 0 495 1704
Size: 495 1209
BsaBI GATnn'nnATC
Cuts at: 0 1585 1704
Size: 1585 119
BsaHI Gr'CG_yC
Cuts at: 0 85 1515 1704
Size: 85 1430 189
Fragments arranged by size:
1430 189 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 490 520 1704
Size: 133 30 327 30 1184
Fragments arranged by size:
1184 327 133 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 657 1704
Size: 657 1047
BseYI C'CCAG_C
Cuts at: 0 79 387 685 1148 1704
Size: 79 308 298 463 556
Fragments arranged by size:
556 463 308 298 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 201 392 642 790 1704
Size: 46 155 191 250 148 914
Fragments arranged by size:
914 250 191 155 148 46
BsiHKAI G_wGCw'C
Cuts at: 0 247 660 1184 1253 1704
Size: 247 413 524 69 451
Fragments arranged by size:
524 451 413 247 69
BsmI GAATG_Cn'
Cuts at: 0 1531 1704
Size: 1531 173
Bsp1286I G_dGCh'C
Cuts at: 0 209 247 660 1184 1253 1704
Size: 209 38 413 524 69 451
Fragments arranged by size:
524 451 413 209 69 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 491 791 1217 1704
Size: 491 300 426 487
Fragments arranged by size:
491 487 426 300
BsrFI r'CCGG_y
Cuts at: 0 1157 1704
Size: 1157 547
BsrGI T'GTAC_A
Cuts at: 0 262 1704
Size: 262 1442
BssHII G'CGCG_C
Cuts at: 0 32 1704
Size: 32 1672
BssSI C'ACGA_G
Cuts at: 0 751 1704
Size: 751 953
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 536 1704
Size: 384 152 1168
Fragments arranged by size:
1168 384 152
BstBI TT'CG_AA
Cuts at: 0 927 1704
Size: 927 777
BstYI r'GATC_y
Cuts at: 0 341 1007 1689 1704
Size: 341 666 682 15
Fragments arranged by size:
682 666 341 15
Bsu36I CC'TnA_GG
Cuts at: 0 640 1704
Size: 640 1064
BtgI C'CryG_G
Cuts at: 0 137 1704
Size: 137 1567
BtsI GCAGTG_nn'
Cuts at: 0 81 534 1704
Size: 81 453 1170
Fragments arranged by size:
1170 453 81
DraI TTT'AAA
Cuts at: 0 410 1704
Size: 410 1294
DraIII CAC_nnn'GTG
Cuts at: 0 174 1704
Size: 174 1530
DrdI GACnn_nn'nnGTC
Cuts at: 0 1410 1704
Size: 1410 294
EaeI y'GGCC_r
Cuts at: 0 1462 1704
Size: 1462 242
EarI CTCTTCn'nnn_
Cuts at: 0 252 341 1045 1704
Size: 252 89 704 659
Fragments arranged by size:
704 659 252 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 753 1180 1296 1704
Size: 753 427 116 408
Fragments arranged by size:
753 427 408 116
EcoICRI GAG'CTC
Cuts at: 0 245 1704
Size: 245 1459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 808 1009 1054 1111 1162 1246 1595
Size: 322 486 201 45 57 51 84 349
Cuts at: 1595 1704
Size: 109
Fragments arranged by size:
486 349 322 201 109 84 57 51
45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 104 787 1704
Size: 104 683 917
Fragments arranged by size:
917 683 104
EcoO109I rG'GnC_Cy
Cuts at: 0 1221 1505 1704
Size: 1221 284 199
Fragments arranged by size:
1221 284 199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 840 1704
Size: 840 864
HaeII r_GCGC'y
Cuts at: 0 708 1704
Size: 708 996
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 333 365 1238 1270 1489 1521
Size: 9 32 292 32 873 32 219 32
Cuts at: 1521 1704
Size: 183
Fragments arranged by size:
873 292 219 183 32 32 32 32
9
HincII GTy'rAC
Cuts at: 0 780 1704
Size: 780 924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1704
Size: 331 1373
MslI CAynn'nnrTG
Cuts at: 0 1492 1704
Size: 1492 212
MspA1I CmG'CkG
Cuts at: 0 1152 1704
Size: 1152 552
NaeI GCC'GGC
Cuts at: 0 1159 1704
Size: 1159 545
NgoMIV G'CCGG_C
Cuts at: 0 1157 1704
Size: 1157 547
NruI TCG'CGA
Cuts at: 0 1268 1704
Size: 1268 436
NsiI A_TGCA'T
Cuts at: 0 1533 1704
Size: 1533 171
NspI r_CATG'y
Cuts at: 0 530 1704
Size: 530 1174
PflMI CCAn_nnn'nTGG
Cuts at: 0 1224 1704
Size: 1224 480
PfoI T'CCnGG_A
Cuts at: 0 1223 1704
Size: 1223 481
PmlI CAC'GTG
Cuts at: 0 495 1704
Size: 495 1209
PpuMI rG'GwC_Cy
Cuts at: 0 1221 1505 1704
Size: 1221 284 199
Fragments arranged by size:
1221 284 199
PstI C_TGCA'G
Cuts at: 0 41 569 1704
Size: 41 528 1135
Fragments arranged by size:
1135 528 41
PvuII CAG'CTG
Cuts at: 0 1152 1704
Size: 1152 552
SacI G_AGCT'C
Cuts at: 0 247 1704
Size: 247 1457
SapI GCTCTTCn'nnn_
Cuts at: 0 252 341 1045 1704
Size: 252 89 704 659
Fragments arranged by size:
704 659 252 89
SexAI A'CCwGG_T
Cuts at: 0 170 1704
Size: 170 1534
SfcI C'TryA_G
Cuts at: 0 37 565 693 1704
Size: 37 528 128 1011
Fragments arranged by size:
1011 528 128 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 754 1704
Size: 754 950
SmlI C'TyrA_G
Cuts at: 0 471 1704
Size: 471 1233
StuI AGG'CCT
Cuts at: 0 1136 1704
Size: 1136 568
StyI C'CwwG_G
Cuts at: 0 1459 1704
Size: 1459 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 1099 1704
Size: 211 888 605
Fragments arranged by size:
888 605 211
TatI w'GTAC_w
Cuts at: 0 149 262 1704
Size: 149 113 1442
Fragments arranged by size:
1442 149 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 490 522 573 605 1704
Size: 490 32 51 32 1099
Fragments arranged by size:
1099 490 51 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1311 1704
Size: 1311 393
XbaI T'CTAG_A
Cuts at: 0 1626 1704
Size: 1626 78
XmnI GAAnn'nnTTC
Cuts at: 0 434 923 1704
Size: 434 489 781
Fragments arranged by size:
781 489 434
ZraI GAC'GTC
Cuts at: 0 1516 1704
Size: 1516 188
Enzymes that do cut:
AatII AccI AcuI AfeI AlwNI ApaLI ApoI BanI
BanII BbsI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV NruI
NsiI NspI PflMI PfoI PmlI PpuMI PstI PvuII
SacI SapI SexAI SfcI SfiI SmlI StuI StyI
TaqII TatI TstI Tth111I XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BclI BmtI Bpu10I BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BstEII
BstXI BstZ17I BtgZI ClaI CspCI EagI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI PacI
PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XcmI XhoI XmaI