Restriction map for
pF1KB4964
(Linear) (Six-base) MAPSORT of: KIBB4964.seq Check: 2605 from: 1 to: 4392
KIBB4964.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AatII G_ACGT'C
Cuts at: 0 424 4392
Size: 424 3968
AccI GT'mk_AC
Cuts at: 0 424 3797 3887 4392
Size: 424 3373 90 505
Fragments arranged by size:
3373 505 424 90
AclI AA'CG_TT
Cuts at: 0 1551 4392
Size: 1551 2841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 676 1411 1577 1719 2278 2323 3745
Size: 496 180 735 166 142 559 45 1422
Cuts at: 3745 4392
Size: 647
Fragments arranged by size:
1422 735 647 559 496 180 166 142
45
AflIII A'CryG_T
Cuts at: 0 254 1282 2678 4353 4392
Size: 254 1028 1396 1675 39
Fragments arranged by size:
1675 1396 1028 254 39
AgeI A'CCGG_T
Cuts at: 0 2275 4392
Size: 2275 2117
AhdI GACnn_n'nnGTC
Cuts at: 0 267 2838 4392
Size: 267 2571 1554
Fragments arranged by size:
2571 1554 267
AleI CACnn'nnGTG
Cuts at: 0 253 2551 4392
Size: 253 2298 1841
Fragments arranged by size:
2298 1841 253
AlwNI CAG_nnn'CTG
Cuts at: 0 336 3647 4392
Size: 336 3311 745
Fragments arranged by size:
3311 745 336
ApaI G_GGCC'C
Cuts at: 0 169 4392
Size: 169 4223
ApaLI G'TGCA_C
Cuts at: 0 1069 2620 4392
Size: 1069 1551 1772
Fragments arranged by size:
1772 1551 1069
ApoI r'AATT_y
Cuts at: 0 544 1969 2119 2817 3966 4335 4392
Size: 544 1425 150 698 1149 369 57
Fragments arranged by size:
1425 1149 698 544 369 150 57
AvaI C'yCGr_G
Cuts at: 0 169 940 3424 4008 4392
Size: 169 771 2484 584 384
Fragments arranged by size:
2484 771 584 384 169
AvrII C'CTAG_G
Cuts at: 0 3145 3895 4392
Size: 3145 750 497
Fragments arranged by size:
3145 750 497
BamHI G'GATC_C
Cuts at: 0 414 2665 3456 4392
Size: 414 2251 791 936
Fragments arranged by size:
2251 936 791 414
BanI G'GyrC_C
Cuts at: 0 63 1624 4392
Size: 63 1561 2768
Fragments arranged by size:
2768 1561 63
BanII G_rGCy'C
Cuts at: 0 169 800 3145 4392
Size: 169 631 2345 1247
Fragments arranged by size:
2345 1247 631 169
BbeI G_GCGC'C
Cuts at: 0 67 4392
Size: 67 4325
BbsI GAAGACnn'nnnn_
Cuts at: 0 498 1265 3755 4392
Size: 498 767 2490 637
Fragments arranged by size:
2490 767 637 498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1511 1545 4392
Size: 1511 34 2847
Fragments arranged by size:
2847 1511 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1741 4392
Size: 1741 2651
BclI T'GATC_A
Cuts at: 0 398 608 4392
Size: 398 210 3784
Fragments arranged by size:
3784 398 210
BfrBI ATG'CAT
Cuts at: 0 2103 4392
Size: 2103 2289
BglI GCCn_nnn'nGGC
Cuts at: 0 1945 2482 4392
Size: 1945 537 1910
Fragments arranged by size:
1945 1910 537
BglII A'GATC_T
Cuts at: 0 3347 4392
Size: 3347 1045
BlpI GC'TnA_GC
Cuts at: 0 1147 4392
Size: 1147 3245
Bme1580I G_kGCm'C
Cuts at: 0 169 279 1073 1580 2624 3816 4392
Size: 169 110 794 507 1044 1192 576
Fragments arranged by size:
1192 1044 794 576 507 169 110
BmgBI CAC'GTC
Cuts at: 0 135 198 1283 4392
Size: 135 63 1085 3109
Fragments arranged by size:
3109 1085 135 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 858 2399 3393 3455 3819 4392
Size: 858 1541 994 62 364 573
Fragments arranged by size:
1541 994 858 573 364 62
BmtI G_CTAG'C
Cuts at: 0 1931 4392
Size: 1931 2461
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 747 945 2803 4082 4392
Size: 747 198 1858 1279 310
Fragments arranged by size:
1858 1279 747 310 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 940 1252 2392 2630 3979 4063 4392
Size: 883 57 312 1140 238 1349 84 329
Fragments arranged by size:
1349 1140 883 329 312 238 84 57
Bpu10I CC'TnA_GC
Cuts at: 0 2488 3850 3910 4392
Size: 2488 1362 60 482
Fragments arranged by size:
2488 1362 482 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2721 3922 3981 4233 4392
Size: 2721 1201 59 252 159
Fragments arranged by size:
2721 1201 252 159 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 786 2007 2368 3598 4392
Size: 786 1221 361 1230 794
Fragments arranged by size:
1230 1221 794 786 361
BsaAI yAC'GTr
Cuts at: 0 255 3206 4392
Size: 255 2951 1186
Fragments arranged by size:
2951 1186 255
BsaHI Gr'CG_yC
Cuts at: 0 64 421 2144 4392
Size: 64 357 1723 2248
Fragments arranged by size:
2248 1723 357 64
BsaWI w'CCGG_w
Cuts at: 0 2275 2693 3503 4392
Size: 2275 418 810 889
Fragments arranged by size:
2275 889 810 418
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2365 2395 2716 2746 4069 4099 4392
Size: 2365 30 321 30 1323 30 293
Fragments arranged by size:
2365 1323 321 293 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 339 1249 2380 2680 2724 4071 4392
Size: 339 910 1131 300 44 1347 321
Fragments arranged by size:
1347 1131 910 339 321 300 44
BseYI C'CCAG_C
Cuts at: 0 117 590 772 2215 3777 4392
Size: 117 473 182 1443 1562 615
Fragments arranged by size:
1562 1443 615 473 182 117
BsiEI CG_ry'CG
Cuts at: 0 2565 4207 4392
Size: 2565 1642 185
Fragments arranged by size:
2565 1642 185
BsiHKAI G_wGCw'C
Cuts at: 0 800 1073 2516 2560 2624 3004 4392
Size: 800 273 1443 44 64 380 1388
Fragments arranged by size:
1443 1388 800 380 273 64 44
BsmI GAATG_Cn'
Cuts at: 0 377 2609 3867 4392
Size: 377 2232 1258 525
Fragments arranged by size:
2232 1258 525 377
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1273 1635 4392
Size: 1273 362 2757
Fragments arranged by size:
2757 1273 362
Bsp1286I G_dGCh'C
Cuts at: 0 169 279 800 1073 1580 2516 2560 2624
Size: 169 110 521 273 507 936 44 64
Cuts at: 2624 3004 3145 3816 4392
Size: 380 141 671 576
Fragments arranged by size:
936 671 576 521 507 380 273 169
141 110 64 44
BspHI T'CATG_A
Cuts at: 0 1955 2019 4392
Size: 1955 64 2373
Fragments arranged by size:
2373 1955 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3783 4392
Size: 3783 609
BsrBI CCG'CTC
Cuts at: 0 731 4392
Size: 731 3661
BsrFI r'CCGG_y
Cuts at: 0 36 1987 2275 2564 2906 3620 4392
Size: 36 1951 288 289 342 714 772
Fragments arranged by size:
1951 772 714 342 289 288 36
BsrGI T'GTAC_A
Cuts at: 0 4358 4392
Size: 4358 34
BssSI C'ACGA_G
Cuts at: 0 292 4392
Size: 292 4100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 999 3647 4284 4392
Size: 999 2648 637 108
Fragments arranged by size:
2648 999 637 108
BstBI TT'CG_AA
Cuts at: 0 153 4392
Size: 153 4239
BstEII G'GTnAC_C
Cuts at: 0 516 1437 2241 3772 4392
Size: 516 921 804 1531 620
Fragments arranged by size:
1531 921 804 620 516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1083 4392
Size: 1083 3309
BstYI r'GATC_y
Cuts at: 0 414 662 2393 2665 3347 3390 3456 4392
Size: 414 248 1731 272 682 43 66 936
Fragments arranged by size:
1731 936 682 414 272 248 66 43
Bsu36I CC'TnA_GG
Cuts at: 0 1153 4392
Size: 1153 3239
BtgI C'CryG_G
Cuts at: 0 285 464 1782 2480 3050 4392
Size: 285 179 1318 698 570 1342
Fragments arranged by size:
1342 1318 698 570 285 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3722 4392
Size: 3722 670
BtsI GCAGTG_nn'
Cuts at: 0 3636 4392
Size: 3636 756
ClaI AT'CG_AT
Cuts at: 0 3743 4392
Size: 3743 649
DraI TTT'AAA
Cuts at: 0 3258 4334 4392
Size: 3258 1076 58
Fragments arranged by size:
3258 1076 58
DraIII CAC_nnn'GTG
Cuts at: 0 135 1164 4392
Size: 135 1029 3228
Fragments arranged by size:
3228 1029 135
DrdI GACnn_nn'nnGTC
Cuts at: 0 502 2149 4392
Size: 502 1647 2243
Fragments arranged by size:
2243 1647 502
EaeI y'GGCC_r
Cuts at: 0 2900 2993 4392
Size: 2900 93 1399
Fragments arranged by size:
2900 1399 93
EarI CTCTTCn'nnn_
Cuts at: 0 675 2308 2524 2584 3155 3518 4392
Size: 675 1633 216 60 571 363 874
Fragments arranged by size:
1633 874 675 571 363 216 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 4392
Size: 94 4298
EcoICRI GAG'CTC
Cuts at: 0 798 4392
Size: 798 3594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 676 883 940 1252 1411 1577 1719
Size: 496 180 207 57 312 159 166 142
Cuts at: 1719 2278 2323 2392 2630 3745 3979 4063 4392
Size: 559 45 69 238 1115 234 84 329
Fragments arranged by size:
1115 559 496 329 312 238 234 207
180 166 159 142 84 69 57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1234 4392
Size: 1234 3158
EcoO109I rG'GnC_Cy
Cuts at: 0 88 165 166 984 1239 1827 2840 3125
Size: 88 77 1 818 255 588 1013 285
Cuts at: 3125 3692 3917 4392
Size: 567 225 475
Fragments arranged by size:
1013 818 588 567 475 285 255 225
88 77 1
EcoRI G'AATT_C
Cuts at: 0 544 4392
Size: 544 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2553 3270 3547 4086 4392
Size: 2553 717 277 539 306
Fragments arranged by size:
2553 717 539 306 277
HaeII r_GCGC'y
Cuts at: 0 67 1664 4392
Size: 67 1597 2728
Fragments arranged by size:
2728 1597 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 253 285 331 363 406 434
Size: 105 32 116 32 46 32 43 28
Cuts at: 434 438 466 802 834 2824 2856 3061 3093
Size: 4 28 336 32 1990 32 205 32
Cuts at: 3093 3332 3364 3846 3878 4069 4101 4392
Size: 239 32 482 32 191 32 291
Fragments arranged by size:
1990 482 336 291 239 205 191 116
105 46 43 32 32 32 32 32
32 32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 0 1467 3888 4220 4392
Size: 1467 2421 332 172
Fragments arranged by size:
2421 1467 332 172
HindIII A'AGCT_T
Cuts at: 0 1396 4392
Size: 1396 2996
HpaI GTT'AAC
Cuts at: 0 1467 4392
Size: 1467 2925
KasI G'GCGC_C
Cuts at: 0 63 4392
Size: 63 4329
MfeI C'AATT_G
Cuts at: 0 1799 4392
Size: 1799 2593
MluI A'CGCG_T
Cuts at: 0 4353 4392
Size: 4353 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1214 1288 1725 2585 2747 2996 3560
Size: 442 772 74 437 860 162 249 564
Cuts at: 3560 3680 4392
Size: 120 712
Fragments arranged by size:
860 772 712 564 442 437 249 162
120 74
MscI TGG'CCA
Cuts at: 0 2902 4392
Size: 2902 1490
MslI CAynn'nnrTG
Cuts at: 0 253 469 2551 2797 4115 4259 4392
Size: 253 216 2082 246 1318 144 133
Fragments arranged by size:
2082 1318 253 246 216 144 133
MspA1I CmG'CkG
Cuts at: 0 654 904 956 2433 2606 3036 4392
Size: 654 250 52 1477 173 430 1356
Fragments arranged by size:
1477 1356 654 430 250 173 52
NaeI GCC'GGC
Cuts at: 0 38 4392
Size: 38 4354
NarI GG'CG_CC
Cuts at: 0 64 4392
Size: 64 4328
NcoI C'CATG_G
Cuts at: 0 1782 2480 4392
Size: 1782 698 1912
Fragments arranged by size:
1912 1782 698
NgoMIV G'CCGG_C
Cuts at: 0 36 4392
Size: 36 4356
NheI G'CTAG_C
Cuts at: 0 1927 4392
Size: 1927 2465
NsiI A_TGCA'T
Cuts at: 0 2105 4392
Size: 2105 2287
NspI r_CATG'y
Cuts at: 0 505 600 2103 2469 2682 2721 3663 4282
Size: 505 95 1503 366 213 39 942 619
Cuts at: 4282 4392
Size: 110
Fragments arranged by size:
1503 942 619 505 366 213 110 95
39
PasI CC'CwG_GG
Cuts at: 0 4325 4392
Size: 4325 67
PciI A'CATG_T
Cuts at: 0 2678 4392
Size: 2678 1714
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 4041 4128 4392
Size: 336 3705 87 264
Fragments arranged by size:
3705 336 264 87
PfoI T'CCnGG_A
Cuts at: 0 3440 3922 4392
Size: 3440 482 470
Fragments arranged by size:
3440 482 470
PmlI CAC'GTG
Cuts at: 0 255 3206 4392
Size: 255 2951 1186
Fragments arranged by size:
2951 1186 255
PpuMI rG'GwC_Cy
Cuts at: 0 88 984 2840 3125 3692 4392
Size: 88 896 1856 285 567 700
Fragments arranged by size:
1856 896 700 567 285 88
PshAI GACnn'nnGTC
Cuts at: 0 646 4392
Size: 646 3746
PspOMI G'GGCC_C
Cuts at: 0 165 4392
Size: 165 4227
PstI C_TGCA'G
Cuts at: 0 3643 4345 4392
Size: 3643 702 47
Fragments arranged by size:
3643 702 47
PvuII CAG'CTG
Cuts at: 0 654 956 2433 2606 3036 4392
Size: 654 302 1477 173 430 1356
Fragments arranged by size:
1477 1356 654 430 302 173
RsrII CG'GwC_CG
Cuts at: 0 58 4392
Size: 58 4334
SacI G_AGCT'C
Cuts at: 0 800 4392
Size: 800 3592
SalI G'TCGA_C
Cuts at: 0 3886 4392
Size: 3886 506
SanDI GG'GwC_CC
Cuts at: 0 88 4392
Size: 88 4304
SapI GCTCTTCn'nnn_
Cuts at: 0 2584 4392
Size: 2584 1808
SexAI A'CCwGG_T
Cuts at: 0 1064 4392
Size: 1064 3328
SfcI C'TryA_G
Cuts at: 0 537 855 2601 3291 3356 3639 4341 4392
Size: 537 318 1746 690 65 283 702 51
Fragments arranged by size:
1746 702 690 537 318 283 65 51
SfoI GGC'GCC
Cuts at: 0 65 4392
Size: 65 4327
SmlI C'TyrA_G
Cuts at: 0 2736 3937 3996 4212 4392
Size: 2736 1201 59 216 180
Fragments arranged by size:
2736 1201 216 180 59
SspI AAT'ATT
Cuts at: 0 105 4392
Size: 105 4287
StyI C'CwwG_G
Cuts at: 0 735 947 1782 2147 2308 2480 3145 3245
Size: 735 212 835 365 161 172 665 100
Cuts at: 3245 3284 3895 4035 4392
Size: 39 611 140 357
Fragments arranged by size:
835 735 665 611 365 357 212 172
161 140 100 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1019 1662 2338 2766 4193 4392
Size: 1019 643 676 428 1427 199
Fragments arranged by size:
1427 1019 676 643 428 199
TatI w'GTAC_w
Cuts at: 0 2108 2825 4313 4358 4392
Size: 2108 717 1488 45 34
Fragments arranged by size:
2108 1488 717 45 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2365 2397 4392
Size: 2365 32 1995
Fragments arranged by size:
2365 1995 32
XbaI T'CTAG_A
Cuts at: 0 1647 3022 4392
Size: 1647 1375 1370
Fragments arranged by size:
1647 1375 1370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1884 4392
Size: 1884 2508
XmnI GAAnn'nnTTC
Cuts at: 0 3953 4392
Size: 3953 439
ZraI GAC'GTC
Cuts at: 0 422 4392
Size: 422 3970
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII RsrII
SacI SalI SanDI SapI SexAI SfcI SfoI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AlfI AloI AscI AseI
AsiSI BaeI BbvCI BsaBI BsgI BsiWI BspEI BsrDI
BssHII BstZ17I CspCI EagI EcoRV FseI FspI FspAI
KpnI NdeI NotI NruI PacI PmeI PpiI PsiI
PspXI PsrI PvuI SacII SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XhoI XmaI